scholarly journals Effect of roasting parameters on the physicochemical characteristics of high-molecular-weight Maillard reaction products isolated from cocoa beans of different Theobroma cacao L. groups

2018 ◽  
Vol 245 (1) ◽  
pp. 111-128 ◽  
Author(s):  
Joanna Oracz ◽  
Ewa Nebesny
2010 ◽  
Vol 120 (2) ◽  
pp. 505-511 ◽  
Author(s):  
Feng-Lin Gu ◽  
Jin Moon Kim ◽  
Shabbar Abbas ◽  
Xiao-Ming Zhang ◽  
Shu-Qin Xia ◽  
...  

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1901
Author(s):  
Hong-Ting Victor Lin ◽  
Der-Sheng Chan ◽  
Ling-Yu Kao ◽  
Wen-Chieh Sung

The aim of this research was to investigate the effects of the addition of 0.5% hydroxymethylfurfural (HMF) and low molecular chitosan on acrylamide and HMF formation in a food model system, which contains 0.5% glucose, asparagine, and HMF within 30 min of heating at 180 °C. At an interval of 10 min, all solutions were evaluated in the following aspects: reducing sugar, asparagine, acrylamide, HMF content, pH, Maillard reaction products, kinematic viscosity, and color. After heating for 10 min, the kinematic viscosity of solutions containing chitosan reduced significantly. The values of the acrylamide, HMF, and absorbance increased at OD294 and OD420 (optical density measured at 294 nm and 420 nm) of solutions. Experimental results showed that low-molecular-weight chitosan might be hydrolyzed into much lower molecular weight, followed by the decrease in kinematic viscosity of the solution at pH lower than 6 and the increase in the formation of acrylamide after heating for 30 min.


2010 ◽  
Vol 8 (1) ◽  
pp. 104-110 ◽  
Author(s):  
Ratna Agung Samsumaharto

Lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) was extracted and purified from acetone dry powder of cocoa (Theobroma cacao. L.) of clone PBC 159 extract. The Lipase  from AcDP of cocoa beans was used for purification using 40-60 and 60-80% ammonium sulphate precipitation. The resulted indicated 44.73 and 60.51-fold purification with 26.74 and 33.31% recovery lipase activity (yield), respectively. The crude lipase enzyme from both precipitation were eluted, producing a single peak after applying through Sephacryl S-200 chromatography. The purified enzyme had a uniform specific activity throughout the final chromatography peak. The results from SDS-PAGE analysis showed that the molecular weight of the lipase enzyme was in between 45-66 kDa.   Keywords: isolation, purification, lipase, cocoa beans


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