Differential Expression Profiles of Circular RNAs During Osteogenic Differentiation of Mouse Adipose-Derived Stromal Cells

Abstract Background Bone disease causes short-term or long-term physical pain and disability. It is necessary to explore new drug for bone-related disease. This study aimed to explore the role and mechanism of Salidroside in promoting osteogenic differentiation of adipose-derived stromal cells (ADSCs). Methods ADSCs were isolated and treated with different dose of Salidroside. Cell count kit-8 (CCK-8) assay was performed to assess the cell viability of ADSCs. Then, ALP and ARS staining were conducted to assess the early and late osteogenic capacity of ADSCs, respectively. Then, differentially expressed genes were obtained by R software. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially expressed genes were further analyzed. The expression of OCN, COL1A1, RUNX2, WNT3A, and β-catenin were measured by real-time PCR and Western blot analysis. Last, β-catenin was silenced by small interfering RNA. Results Salidroside significantly increased the ADSCs viability at a dose-response manner. Moreover, Salidroside enhanced osteogenic capacity of ADSCs, which are identified by enhanced ALP activity and calcium deposition. A total of 543 differentially expressed genes were identified between normal and Salidroside-treated ADSCs. Among these differentially expressed genes, 345 genes were upregulated and 198 genes were downregulated. Differentially expressed genes enriched in the Wnt/β-catenin signaling pathway. Western blot assay indicated that Salidroside enhanced the WNT3A and β-catenin expression. Silencing β-catenin partially reversed the promotion effects of Salidroside. PCR and Western blot results further confirmed these results. Conclusion Salidroside promoted osteogenic differentiation of ADSCs through Wnt/β-catenin signaling pathway.

Download Full-text

10:57 OPA 2. Osteogenic Differentiation of Adipose-Derived Stromal Cells Treated With GDF-5 Cultured on a Novel Three-Dimensional Sintered Microsphere Matrix

The Spine Journal ◽

10.1016/j.spinee.2006.06.149 ◽

2006 ◽

Vol 6 (5) ◽

pp. 58S-59S

Author(s):

Francis H. Shen ◽

Qing Zeng ◽

Qing Lv ◽

Luke Choi ◽

Gary Balian ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Stromal Cells ◽

Three Dimensional ◽

Adipose Derived Stromal Cells

Download Full-text

Characterization of Human Knee and Chin Adipose-Derived Stromal Cells

Stem Cells International ◽

10.1155/2015/592090 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 12

Author(s):

Magali Kouidhi ◽

Phi Villageois ◽

Carine M. Mounier ◽

Corinne Ménigot ◽

Yves Rival ◽

...

Keyword(s):

Stromal Cells ◽

Expression Profiles ◽

Donor Site ◽

Subcutaneous Fat ◽

Gene Expression Profiles ◽

Fat Grafting ◽

Human Knee ◽

Fat Depots ◽

Adipose Derived Stromal Cells ◽

Molecular Matching

Animal study findings have revealed that individual fat depots are not functionally equivalent and have different embryonic origins depending on the anatomic location. Mouse bone regeneration studies have also shown that it is essential to match theHoxcode of transplanted cells and host tissues to achieve correct repair. However, subcutaneous fat depots from any donor site are often used in autologous fat grafting. Our study was thus carried out to determine the embryonic origins of human facial (chin) and limb (knee) fat depots and whether they had similar features and molecular matching patterns. Paired chin and knee fat depots were harvested from 11 subjects and gene expression profiles were determined by DNA microarray analyses. Adipose-derived stromal cells (ASCs) from both sites were isolated and analyzed for their capacity to proliferate, form clones, and differentiate. Chin and knee fat depots expressed a differentHOXcode and could have different embryonic origins. ASCs displayed a different phenotype, with chin-ASCs having the potential to differentiate into brown-like adipocytes, whereas knee-ASCs differentiated into white adipocytes. These results highlighted different features for these two fat sites and indicated that donor site selection might be an important factor to be considered when applying adipose tissue in cell-based therapies.

Download Full-text

Differential expression profiles and functional analysis of circular RNAs in children with fulminant myocarditis

Epigenomics ◽

10.2217/epi-2019-0101 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1129-1141 ◽

Cited By ~ 5

Author(s):

Li Zhang ◽

Bo Han ◽

Jing Wang ◽

Qingqing Liu ◽

Yaru Kong ◽

...

Keyword(s):

Functional Analysis ◽

Healthy Volunteers ◽

Differential Expression ◽

Expression Profiles ◽

Interaction Network ◽

Fulminant Myocarditis ◽

Circular Rnas ◽

Biological Functions ◽

Microarray Experiments ◽

Network Building

Aim: To assess differential expression profiles of circular RNAs (circRNAs) and explore their possible functions in children with fulminant myocarditis. Materials & methods: circRNA microarray experiments were carried out for determining differential expression profiles of circRNAs in three children with fulminant myocarditis and three healthy volunteers. Functional analysis and circRNA–miRNA–mRNA interaction network building were conducted to study biological functions. Results: This work identified 2281 upregulated and 892 downregulated circRNAs. Further assessment confirmed hsa_circ_0071542 upregulation (2.5-fold) in fulminant myocarditis. Functional analysis demonstrated the differentially expressed circRNAs mainly contributed to inflammation and immunity. Conclusion: circRNAs might have substantial roles in pediatric fulminant myocarditis, and hsa_circ_0071542 could serve as a promising biomarker.

Download Full-text