The Oral Bacterium Fusobacterium nucleatum Binds Staphylococcus aureus and Alters Expression of the Staphylococcal Accessory Regulator sarA

2018 ◽  
Vol 78 (2) ◽  
pp. 336-347 ◽  
Author(s):  
Bruno P. Lima ◽  
Linda I. Hu ◽  
Gerrit W. Vreeman ◽  
Douglas B. Weibel ◽  
Renate Lux
Keyword(s):  
Staphylococcus Aureus ◽  
Fusobacterium Nucleatum ◽  
Oral Bacterium

scholarly journals β-Glucanase Activity of the Oral Bacterium Tannerella forsythia Contributes to the Growth of a Partner Species, Fusobacterium nucleatum, in Cobiofilms

2017 ◽  
Vol 84 (1) ◽  
Author(s):  
Kiyonobu Honma ◽  
Angela Ruscitto ◽  
Ashu Sharma
Keyword(s):  
Periodontal Disease ◽  
Sigma Factor ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Disease Pathogenesis ◽  
Glucanase Activity ◽  
Tannerella Forsythia ◽  
Content Type ◽  
Plaque Development ◽  
Oral Bacterium

ABSTRACTTannerella forsythiaandFusobacterium nucleatumare dental plaque bacteria implicated in the development of periodontitis. These two species have been shown to form synergistic biofilms and have been found to be closely associated in dental plaque biofilms. A number of genetic loci for TonB-dependent membrane receptors (TDR) for glycan acquisition, with many existing in association with genes coding for enzymes involved in the breakdown of complex glycans, have been identified inT. forsythia. In this study, we focused on a locus, BFO_0186-BFO_0188, that codes for a predicted TDR-SusD transporter along with a putative β-glucan hydrolyzing enzyme (BFO_0186). This operon is located immediately downstream of a 2-gene operon that codes for a putative stress-responsive extracytoplasmic function (ECF) sigma factor and an anti-sigma factor. Here, we show that BFO_0186 expresses a β-glucanase that cleaves glucans with β-1,6 and β-1,3 linkages. Furthermore, the BFO_0186-BFO_0188 locus is upregulated, with an induction of β-glucanase activity, in cobiofilms ofT. forsythiaandF. nucleatum. The β-glucanase activity in mixed biofilms in turn leads to an enhanced hydrolysis of β-glucans and release of glucose monomers and oligomers as nutrients forF. nucleatum. In summary, our study highlights the role ofT. forsythiaβ-glucanase expressed by the asaccharolytic oral bacteriumT. forsythiain the development ofT. forsythia-F. nucleatummixed species biofilms, and suggest that dietary β-glucans might contribute in plaque development and periodontal disease pathogenesis.IMPORTANCEThe development of dental plaque biofilm is a complex process in which metabolic, chemical and physical interactions between bacteria take a central role. Previous studies have shown that the dental pathogensT. forsythiaandF. nucleatumform synergistic biofilms and are closely associated in human dental plaque. In this study, we show that β-glucanase from the periodontal pathogenT. forsythiaplays a role in the formation ofT. forsythia-F. nucleatumcobiofilms by hydrolyzing β-glucans to glucose as a nutrient. We also unveiled that the expression ofT. forsythiaβ-glucanase is induced in response toF. nucleatumsensing. This study highlights the involvement of β-glucanase activity in the development ofT. forsythia-F. nucleatumbiofilms and suggests that intake of dietary β-glucans might be a contributing risk factor in plaque development and periodontal disease pathogenesis.

scholarly journals Staphylococcus aureus Interferes with Streptococci Spatial Distribution and with Protein Expression of Species within a Polymicrobial Oral Biofilm

Antibiotics ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 116
Author(s):  
Etyene Schnurr ◽  
Pune N. Paqué ◽  
Thomas Attin ◽  
Paolo Nanni ◽  
Jonas Grossmann ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Laser Scanning ◽  
Bacterial Species ◽  
Fusobacterium Nucleatum ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Oral Diseases ◽  
Streptococcus Oralis ◽  
Oral Environment ◽  
Associated Species

We asked whether transient Staphylococcus aureus in the oral environment synergistically interacts with orally associated bacterial species such as Actinomyces oris, Candida albicans, Fusobacterium nucleatum, Streptococcus oralis, Streptococcus mutans, and Veillonella dispar (six-species control biofilm 6S). For this purpose, four modified biofilms with seven species that contain either the wild type strain of the S. aureus genotype (USA300-MRSA WT), its isogenic mutant with MSCRAMM deficiency (USA300-MRSA ΔMSCRAMM), a methicillin-sensitive S. aureus (ST72-MSSA-) or a methicillin-resistant S. aureus (USA800-MRSA) grown on hydroxyapatite disks were examined. Culture analyses, confocal-laser-scanning microscopy and proteome analyses were performed. S. aureus strains affected the amount of supragingival biofilm-associated species differently. The deletion of MSCRAMM genes disrupted the growth of S. aureus and the distribution of S. mutans and S. oralis within the biofilms. In addition, S. aureus caused shifts in the number of detectable proteins of other species in the 6S biofilm. S. aureus (USA300-MRSA WT), aggregated together with early colonizers such as Actinomyces and streptococci, influenced the number of secondary colonizers such as Fusobacterium nucleatum and was involved in structuring the biofilm architecture that triggered the change from a homeostatic biofilm to a dysbiotic biofilm to the development of oral diseases.

scholarly journals NEW OPPORTUNITIES LOCAL MEDICAL TREATMENT OF PERIODONTAL DISEASES (MICROBIOLOGICAL RATIONALE)

2018 ◽  
Vol 22 (4) ◽  
pp. 180-183
Author(s):  
A. N Kalinina ◽  
I. S Lasko ◽  
V. N Tsarev ◽  
Egor Evgen'evich Olesov ◽  
A. F Stepanov ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stationary Phase ◽  
Periodontal Diseases ◽  
Fusobacterium Nucleatum ◽  
Lag Phase ◽  
Periodontal Pathogens ◽  
Streptococcus Constellatus ◽  
Comparison Criteria ◽  
Peak Phase ◽  
Geometric Growth

The article presents the results of a microbiological experiment to study the sensitivity of periodontal pathogens to coniferous polyprenols in the preparation of “Solagift”. The optica density of clinical isolates Streptococcus constellatus; Staphylococcus aureus; Fusobacterium nucleatum; Aggregatibacter actinomycetemcomitans during cultivation with the addition of polyprenol concentrate 1:5 was measured during 3-7 days. Comparison criteria: the change in the phase of adaptation (lag-phase), the phase change of geometric growth, the amplitude of the peak phase of geometric growth, the duration of the stationary phase, the period of the withering away of culture. In comparison with the parameters of periodontal pathogens in the control, the presence of coniferous polyprenols led to a significant decrease in the activity of all microbes according to all criteria, especially Staphylococcus aureus, whose growth was completely suppressed.

scholarly journals Induction of Apoptotic Cell Death in Peripheral Blood Mononuclear and Polymorphonuclear Cells by an Oral Bacterium,Fusobacterium nucleatum

2000 ◽  
Vol 68 (4) ◽  
pp. 1893-1898 ◽  
Author(s):  
Anahid Jewett ◽  
Wyatt R. Hume ◽  
Ho Le ◽  
Tri N. Huynh ◽  
Yiping W. Han ◽  
...  
Keyword(s):  
Cell Death ◽  
Periodontal Disease ◽  
Peripheral Blood ◽  
Cell Apoptosis ◽  
Apoptotic Cell ◽  
Fusobacterium Nucleatum ◽  
Polymorphonuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Oral Bacterium

ABSTRACT It is largely unknown why a variety of bacteria present in the oral cavity are capable of establishing themselves in the periodontal pockets of nonimmunocompromised individuals in the presence of competent immune effector cells. In this paper we present evidence for the immunosuppressive role of Fusobacterium nucleatum, a gram-negative oral bacterium which plays an important role in the generation of periodontal disease. Our studies indicate that the immunosuppressive role of F. nucleatum is largely due to the ability of this organism to induce apoptotic cell death in peripheral blood mononuclear cells (PBMCs) and in polymorphonuclear cells (PMNs). F. nucleatum treatment induced apoptosis of PBMCs and PMNs as assessed by an increase in subdiploid DNA content determined by DNA fragmentation and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling assays. The ability of F. nucleatum to induce apoptosis was abolished by either heat treatment or proteinase digestion but was retained after formaldehyde treatment, suggesting that a heat-labile surface protein component is responsible for bacterium-mediated cell apoptosis. The data also indicated that F. nucleatum-induced cell apoptosis requires activation of caspases and is protected by NF-κB. Possible mechanisms of F. nucleatum's role in the pathogenesis of periodontal disease are discussed.

scholarly journals PARVIMONAS MICRA AND FUSOBACTERIUM NUCLEATUM SEPTIC ARTHRITIS: A RARE ANAEROBIC DOUBLE TROUBLE

2018 ◽  
Vol 11 (12) ◽  
pp. 5
Author(s):  
Padmaja Ananth Shenoy ◽  
Rohit Gupta ◽  
Shashidhar Vishwanath ◽  
Monappa A Naik ◽  
Seema Shetty ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Septic Arthritis ◽  
Fusobacterium Nucleatum ◽  
Medical Attention ◽  
Aerobic Bacteria ◽  
Gram Stain ◽  
Anaerobic Culture ◽  
Aerobic Culture ◽  
Parvimonas Micra ◽  
Hematogenous Route

Septic arthritis is a condition initiated by pathogenic inoculation of joints either by direct or hematogenous route, necessitating immediate medical attention. Among aerobic bacteria, Staphylococcus aureus and Streptococcus spp. are commonly found in association with septic joints. Anaerobes are very rarely involved in the causation of septic arthritis with an estimated rate of <1%. We are presenting a case of septic arthritis of knee joint by Parvimonas micra and Fusobacterium nucleatum, both being constituents of microbial flora in the oral cavity and gastrointestinal tract. Gram stain and anaerobic culture incorporated along with the aerobic culture of synovial fluid have played an important role in the preliminary diagnosis of anaerobic septic arthritis in this case.

scholarly journals LDOC1 Suppresses Microbe-Induced Production of IL-1β in Human Normal and Cancerous Oral Cells through the PI3K/Akt/GSK-3β Axis

Cancers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3148
Author(s):  
Chia-Huei Lee ◽  
Pin-Feng Hung ◽  
Ko-Jiunn Liu ◽  
Hsuan-Lien Chung ◽  
Wen-Chan Yang ◽  
...  
Keyword(s):  
Fusobacterium Nucleatum ◽  
Poor Oral Hygiene ◽  
Pathogenic Role ◽  
Tobacco Carcinogen ◽  
Phosphorylated Akt ◽  
Oral Bacterium ◽  
Oral Microbes ◽  
Gsk 3Β ◽  
Oral Cells

Poor oral hygiene (POH) is associated with oral squamous cell carcinoma (OSCC). Oral microbes often proliferate due to POH. Array data show that LDOC1 plays a role in immunity against pathogens. We investigated whether LDOC1 regulates the production of oral microbe-induced IL-1β, an oncogenic proinflammatory cytokine in OSCC. We demonstrated the presence of Candida albicans (CA) in 11.3% of OSCC tissues (n = 80). CA and the oral bacterium Fusobacterium nucleatum stimulate higher levels of IL-1β secretion by LDOC1-deficient OSCC cells than by LDOC1-expressing oral cells. CA SC5314 increased OSCC incidence in 4-NQO (a synthetic tobacco carcinogen) and arecoline-cotreated mice. Loss and gain of LDOC1 function significantly increased and decreased, respectively, CA SC5314-induced IL-1β production in oral and OSCC cell lines. Mechanistic studies showed that LDOC1 deficiency increased active phosphorylated Akt upon CA SC5314 stimulation and subsequent inhibitory phosphorylation of GSK-3βS9 by activated Akt. PI3K and Akt inhibitors and expression of the constitutively active mutant GSK-3βS9A significantly reduced the CA SC5314-stimulated IL-1β production in LDOC1-deficient cells. These results indicate that the PI3K/Akt/pGSK-3β signaling pathway contributes to LDOC1-mediated inhibition of oral microbe-induced IL-1β production, suggesting that LDOC1 may determine the pathogenic role of oral microbes in POH-associated OSCC.

scholarly journals Genome Sequence and Analysis of the Oral Bacterium Fusobacterium nucleatum Strain ATCC 25586

2002 ◽  
Vol 184 (7) ◽  
pp. 2005-2018 ◽  
Author(s):  
Vinayak Kapatral ◽  
Iain Anderson ◽  
Natalia Ivanova ◽  
Gary Reznik ◽  
Tamara Los ◽  
...  
Keyword(s):  
Amino Acids ◽  
Outer Membrane Proteins ◽  
Gc Content ◽  
Fusobacterium Nucleatum ◽  
Open Reading Frames ◽  
Strain Atcc ◽  
Circular Chromosome ◽  
Oral Bacterium ◽  
Gingival Epithelium ◽  
Key Aspects

ABSTRACT We present a complete DNA sequence and metabolic analysis of the dominant oral bacterium Fusobacterium nucleatum. Although not considered a major dental pathogen on its own, this anaerobe facilitates the aggregation and establishment of several other species including the dental pathogens Porphyromonas gingivalis and Bacteroides forsythus. The F. nucleatum strain ATCC 25586 genome was assembled from shotgun sequences and analyzed using the ERGO bioinformatics suite (http://www.integratedgenomics.com ). The genome contains 2.17 Mb encoding 2,067 open reading frames, organized on a single circular chromosome with 27% GC content. Despite its taxonomic position among the gram-negative bacteria, several features of its core metabolism are similar to that of gram-positive Clostridium spp., Enterococcus spp., and Lactococcus spp. The genome analysis has revealed several key aspects of the pathways of organic acid, amino acid, carbohydrate, and lipid metabolism. Nine very-high-molecular-weight outer membrane proteins are predicted from the sequence, none of which has been reported in the literature. More than 137 transporters for the uptake of a variety of substrates such as peptides, sugars, metal ions, and cofactors have been identified. Biosynthetic pathways exist for only three amino acids: glutamate, aspartate, and asparagine. The remaining amino acids are imported as such or as di- or oligopeptides that are subsequently degraded in the cytoplasm. A principal source of energy appears to be the fermentation of glutamate to butyrate. Additionally, desulfuration of cysteine and methionine yields ammonia, H2S, methyl mercaptan, and butyrate, which are capable of arresting fibroblast growth, thus preventing wound healing and aiding penetration of the gingival epithelium. The metabolic capabilities of F. nucleatum revealed by its genome are therefore consistent with its specialized niche in the mouth.

scholarly journals Estudio microbiológico en implantes inmediatos postexodoncia en alvéolos con lesiones periapicales

2015 ◽  
Author(s):  
◽  
César Gabriel Luchetti
Keyword(s):  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Fusobacterium Nucleatum ◽  
Los Grupos

La colocación inmediata de implantes dentales postexodoncia en alvéolos infectados sigue siendo un tema controvertido en implantología oral. Objetivos: Evaluar los resultados de la colocación inmediata de implantes dentales en alvéolos infectados con respecto al nivel del hueso marginal y la estabilidad del implante en comparación con implantes colocados en los alvéolos sanos y evaluar un procedimiento de limpieza a fin de eliminar los microorganismos presentes en estas situaciones. Material y Métodos: Fueron seleccionados cincuenta (50) pacientes con dientes con infecciones crónicas que requieren extracción y recibieron 50 implantes dentales. (Grupo E). Todos los casos fueron dientes unirradiculares en el maxilar superior. Se evaluaron los aspectos microbiológicos y el procedimiento de limpieza tomando una secuencia de muestras para cultivos de la siguiente manera: 1-Muestra del fluido crevicular. 2- Muestra luego de la extracción del diente. 3- Muestra después del desbridamiento utilizando curetas manuales y 4- Muestra después de aplicar ácido cítrico al 2% durante 1 minuto. Después de esto, los implantes fueron colocados y se tomaron Valores Periotest (VPT) iniciales y el nivel de hueso marginal. A los 4 meses (segunda fase), antes de iniciar la parte protésica, se registraron nuevos valores Periotest y nivel de hueso marginal. Otros datos se obtuvieron a los 12 y 24 meses. Cincuenta implantes colocados en alvéolos sanos en cincuenta pacientes sirvieron como control. (Grupo C) Resultados: Se produjo un fracaso en cada grupo durante el período de evaluación. Los valores Periotest (VPT) promedio en el grupo E fueron: Inicial: -3,19 (0,66), 2da Fase: -3,77 (0,29), 12 meses: -3.89 (0,15) y 24 meses: -3,95 (0,12). Mientras que los valores Periotest promedio en el grupo E fueron: Inicial: -3,25 (0,90), 2da Fase: -3,91 (0,72), 12 meses: -3,99 (0,23) y 24 meses: -4,03 (0,27). No se encontraron diferencias estadísticamente significativas entre los grupos. El nivel óseo marginal fue considerado cero al inicio, con el fin de evaluar luego los cambios. El nivel óseo promedio, en milímetros, en el grupo E fue: 2da Fase: -0,21 (0,12), 12 meses: -0,52 (0,35) y 24 meses: -0,61 (0,24). El nivel óseo promedio, en milímetros, en el grupo C fue: 2da Fase: -0,18 (0,07), 12 meses: -0,55 (0,17) y 24 meses: -0,67 (0,23). No se encontraron diferencias estadísticamente significativas entre los grupos. Los microorganismos más comúnmente encontrados fueron Streptococos grupos C, H (S. sanguis) y K (S. salivarius), Staphylococcus aureus, Bacteroides forsythus and Fusobacterium nucleatum. También se observó Candida albicans en algunas muestras. Los antimicrobianos más eficaces fueron ciprofloxacina, amoxicilina más ácido clavulánico y metronidazol. Fluconazol fue el antimicótico más eficaz. El desbridamiento manual con curetas no fue capaz de producir una limpieza adecuada del alveolo. Sin embargo, la misma mejoró después de la aplicación de ácido cítrico al 2% como se demostró en los cultivos. Conclusiones: Dentro de las limitaciones del presente estudio, la colocación de implantes inmediatos en alvéolos infectados podría ser considerado un procedimiento predecible. No hubo diferencias estadísticamente significativas en comparación con los implantes colocados en los alvéolos sanos. El desbridamiento manual por sí solo no fue suficiente para realizar una limpieza adecuada. En este estudio, el ácido cítrico al 2% mostró resultados interesantes en cuanto a control microbiológico. Los Valores Periotest mejoraron durante los 2 años de seguimiento. El nivel del hueso marginal mostró una cierta pérdida, pero esta fue similar en ambos grupos.

scholarly journals Antimicrobial activity of different concentrations of NaOCl and chlorhexidine using a contact test

2003 ◽  
Vol 14 (2) ◽  
pp. 99-102 ◽  
Author(s):  
Luciana Moura Sassone ◽  
Rivail Antonio Sergio Fidel ◽  
Sandra Rivera Fidel ◽  
Marina Dias ◽  
Raphael Hirata Junior
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Fusobacterium Nucleatum ◽  
Time Interval ◽  
Time Intervals ◽  
Contact Test

The purpose of this study was to analyze the in vitro antimicrobial activity of sodium hypochlorite (1% and 5%) and chlorhexidine (0.12%, 0.5% and 1%). Bacterial samples (ATCC) of Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Porphyromonas gingivalis and Fusobacterium nucleatum were submitted to a contact test. Solutions were evaluated at different time intervals: immediately, 5 min, 15 min, and 30 min after contact and repeated 10 times. The results of the contact test showed that 0.12% chlorhexidine did not eliminate E. faecalis at any time interval, while 0.5% and 1% chlorhexidine and 1% and 5% sodium hypochlorite did. These results permit us to conclude that to obtain better antimicrobial activity, chlorhexidine in a concentration greater than 0.12% should be used.

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