scholarly journals Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection

2015 ◽  
Vol 99 (22) ◽  
pp. 9685-9698 ◽  
Author(s):  
Ying Wang ◽  
Lulu Si ◽  
Yayan Luo ◽  
Xiaolan Guo ◽  
Junmei Zhou ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Reverse Genetics ◽  
Encephalitis Virus ◽  
Antibody Dependent Enhancement

scholarly journals Human MxB Inhibits the Replication of Hepatitis C Virus

2018 ◽  
Vol 93 (1) ◽  
Author(s):  
Dong-Rong Yi ◽  
Ni An ◽  
Zhen-Long Liu ◽  
Feng-Wen Xu ◽  
Kavita Raniga ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dengue Virus ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Rna Replication ◽  
Encephalitis Virus ◽  
Common Mechanism ◽  
Type I ◽  
Dependent Manner

ABSTRACTType I interferon (IFN) inhibits viruses by inducing the expression of antiviral proteins. The IFN-induced myxovirus resistance B (MxB) protein has been reported to inhibit a limited number of viruses, including HIV-1 and herpesviruses, but its antiviral coverage remains to be explored further. Here we show that MxB interferes with RNA replication of hepatitis C virus (HCV) and significantly inhibits viral replication in a cyclophilin A (CypA)-dependent manner. Our data further show that MxB interacts with the HCV protein NS5A, thereby impairing NS5A interaction with CypA and NS5A localization to the endoplasmic reticulum, two events essential for HCV RNA replication. Interestingly, we found that MxB significantly inhibits two additional CypA-dependent viruses of theFlaviviridaefamily, namely, Japanese encephalitis virus and dengue virus, suggesting a potential link between virus dependence on CypA and virus susceptibility to MxB inhibition. Collectively, these data have identified MxB as a key factor behind IFN-mediated suppression of HCV infection, and they suggest that other CypA-dependent viruses may also be subjected to MxB restriction.IMPORTANCEViruses of theFlaviviridaefamily cause major illness and death around the world and thus pose a great threat to human health. Here we show that IFN-inducible MxB restricts several members of theFlaviviridae, including HCV, Japanese encephalitis virus, and dengue virus. This finding not only suggests an active role of MxB in combating these major pathogenic human viruses but also significantly expands the antiviral spectrum of MxB. Our study further strengthens the link between virus dependence on CypA and susceptibility to MxB restriction and also suggests that MxB may employ a common mechanism to inhibit different viruses. Elucidating the antiviral functions of MxB advances our understanding of IFN-mediated host antiviral defense and may open new avenues to the development of novel antiviral therapeutics.

scholarly journals A dataset of distribution and diversity of mosquito-associated viruses and their mosquito vectors in China

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Evans Atoni ◽  
Lu Zhao ◽  
Cheng Hu ◽  
Nanjie Ren ◽  
Xiaoyu Wang ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Mosquito Species ◽  
Encephalitis Virus ◽  
Health Concern ◽  
Mosquito Vector ◽  
Mosquito Vectors ◽  
Culex Flavivirus ◽  
Conference Papers

Abstract Mosquito-borne viruses such as Zika virus, Japanese Encephalitis virus and Dengue virus present an increasing global health concern. However, in-depth knowledge of the distribution and diversity of mosquito-associated viruses and their related vectors remains limited, especially for China. To promote their understanding, we present the first comprehensive dataset of the distribution and diversity of these viruses and their related vectors in China (including Taiwan, Hong Kong and Macau). Data was drawn from peer-reviewed journal articles, conference papers and thesis publications in both English and Chinese. Geographical data on mosquito-associated viruses’ occurrence and related mosquito vector species was extracted, and quality-control processes employed. This dataset contains 2,428 accounts of mosquito-associated viruses’ and mosquito species geo-referenced occurrences at various administrative levels in China. The prevalent mosquito-associated virus includes Japanese encephalitis virus, Dengue virus, Banna virus and Culex flavivirus, whereas the abundant mosquito vectors are Culex tritaeryohynchus, Aedes albopictus and Culex pipiens pallens. This geographical dataset delivers a distribution and diversity outline of mosquito-associated viruses in China, and also applicable in various spatial and risk-assessment analysis.

scholarly journals Development of a reverse genetics system for Japanese encephalitis virus strain SA14-14-2

Virus Genes ◽  
2019 ◽  
Vol 55 (4) ◽  
pp. 550-556
Author(s):  
Guohua Li ◽  
Hongli Jin ◽  
Xin Nie ◽  
Yongkun Zhao ◽  
Na Feng ◽  
...  
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Virus Strain ◽  
Reverse Genetics ◽  
Encephalitis Virus

scholarly journals Discordant Activity of Kaempferol Towards Dengue Virus and Japanese Encephalitis Virus

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1246
Author(s):  
Chit Care ◽  
Wannapa Sornjai ◽  
Janejira Jaratsittisin ◽  
Atitaya Hitakarun ◽  
Nitwara Wikan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Protein Expression ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Denv Infection ◽  
Significant Inhibition ◽  
Encephalitis Virus ◽  
Host Protein ◽  
Cell Protein ◽  
Infected Cells

Kaempferol, a plant-derived flavonoid, has been reported to have activity against Japanese encephalitis virus (JEV) in BHK-21 cells. To determine the broader utility of this compound, we initially evaluated the activity of kaempferol against JEV and dengue virus (DENV) in HEK293T/17 cells. Results showed no significant antiviral activity against either virus. We subsequently investigated the activity of kaempferol against both JEV and DENV in BHK-21 cells. Results showed a significant inhibition of JEV infection but, surprisingly, a significant enhancement of DENV infection. The effect of kaempferol on both host protein expression and transcription was investigated and both transcriptional and translational inhibitory effects were observed, although a more marked effect was observed on host cell protein expression. Markedly, while GRP78 was increased in DENV infected cells treated with kaempferol, it was not increased in JEV infected cells treated with kaempferol. These results show that cellular alteration induced by one compound can have opposite effects on viruses from the same family, suggesting the presence of distinct replication strategies for these two viruses.

scholarly journals Development and Application of a Reverse Genetics System for Japanese Encephalitis Virus

2003 ◽  
Vol 77 (11) ◽  
pp. 6450-6465 ◽  
Author(s):  
Sang-Im Yun ◽  
Seok-Yong Kim ◽  
Charles M. Rice ◽  
Young-Min Lee
Keyword(s):  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Reverse Genetics ◽  
Heterologous Gene Expression ◽  
High Titer ◽  
Single Point ◽  
Encephalitis Virus ◽  
Full Length ◽  
Plaque Morphology ◽  
Single Point Mutation

ABSTRACT Japanese encephalitis virus (JEV) is a common agent of viral encephalitis that causes high mortality and morbidity among children. Molecular genetic studies of JEV are hampered by the lack of a genetically stable full-length infectious JEV cDNA clone. We describe here the development of such a clone. A JEV isolate was fully sequenced, and then its full-length cDNA was cloned into a bacterial artificial chromosome. This was then further engineered so that transcription of the cDNA in vitro would generate synthetic RNAs with authentic 5′ and 3′ ends. The synthetic RNAs thus produced were highly infectious in susceptible cells (>106 PFU/μg), and these cells rapidly generated a high titer of synthetic viruses (>5 × 106 PFU/ml). The recovered viruses were indistinguishable from the parental virus in terms of plaque morphology, growth kinetics, RNA accumulation, protein expression, and cytopathogenicity. Significantly, the structural and functional integrity of the cDNA was maintained even after 180 generations of growth in Escherichia coli. A single point mutation acting as a genetic marker was introduced into the cDNA and was found in the genome of the recovered virus, indicating that the cDNA can be manipulated. Furthermore, we showed that JEV is an attractive vector for the expression of heterologous genes in a wide variety of cell types. This novel reverse genetics system for JEV will greatly facilitate research into JEV biology. It will also be useful as a heterologous gene expression vector and will aid the development of a vaccine against JEV.

scholarly journals Evaluation of a New Commercially Available Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Japanese Encephalitis Infections

1999 ◽  
Vol 37 (11) ◽  
pp. 3738-3741 ◽  
Author(s):  
Andrea J. Cuzzubbo ◽  
Timothy P. Endy ◽  
David W. Vaughn ◽  
Tom Solomon ◽  
Ananda Nisalak ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Dengue Virus ◽  
Japanese Encephalitis Virus ◽  
Japanese Encephalitis ◽  
Immunosorbent Assay ◽  
Encephalitis Virus ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Commercial Enzyme

A new commercial enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Japanese encephalitis virus infections showed a sensitivity of 88% with sera and 81% with cerebrospinal fluid and a specificity of 97% with sera from patients with primary and secondary dengue virus infections. Specificity was 100% when samples from nonflavivirus infections were tested.

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