scholarly journals Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics

2011 ◽  
Vol 135 (3) ◽  
pp. 293-304 ◽  
Author(s):  
Wanakee J. Carr ◽  
Rebecca E. Oberley-Deegan ◽  
Yuping Zhang ◽  
Christopher C. Oberley ◽  
Larry W. Oberley ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cell ◽  
Side Population ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidant Proteins

scholarly journals A reactive oxygen species-generating, cyclooxygenase-2 inhibiting, cancer stem cell-potent tetranuclear copper(ii) cluster

2017 ◽  
Vol 46 (38) ◽  
pp. 12785-12789 ◽  
Author(s):  
C. Lu ◽  
K. Laws ◽  
A. Eskandari ◽  
K. Suntharalingam
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cells ◽  
Schiff Base ◽  
Stem Cell ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Cancer Cells ◽  
Reactive Oxygen ◽  
Cyclooxygenase 2 ◽  
Oxygen Species

Tetranuclear copper(ii) complexes containing multiple diclofenac and Schiff base moieties,1–4, are shown to kill bulk cancer cells and cancer stem cells (CSCs) with low micromolar potency.

scholarly journals A reactive oxygen species-generating, cancer stem cell-potent manganese(ii) complex and its encapsulation into polymeric nanoparticles

2019 ◽  
Vol 10 (33) ◽  
pp. 7792-7800 ◽  
Author(s):  
Arvin Eskandari ◽  
Kogularamanan Suntharalingam
Keyword(s):  
Breast Cancer ◽  
Reactive Oxygen Species ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Polymeric Nanoparticles ◽  
Reactive Oxygen ◽  
Breast Cancer Stem Cell ◽  
Oxygen Species ◽  
Inflammatory Drug ◽  
Phenanthroline Complex

Here we report the breast cancer stem cell (CSC) potency of a reactive oxygen species (ROS)-generating manganese(ii)-phenanthroline complex bearing diclofenac, a nonsteriodial anti-inflammatory drug.

scholarly journals Root meristem growth factor 1 controls root meristem size through reactive oxygen species signaling

2018 ◽  
Author(s):  
Masashi Yamada ◽  
Xinwei Han ◽  
Philip. N. Benfey
Keyword(s):  
Reactive Oxygen Species ◽  
Transcription Factor ◽  
Stem Cell ◽  
Growth Factor ◽  
Root Meristem ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Meristem Size ◽  
Essential Question ◽  
Meristem Development

AbstractStem cell niche and root meristem size are maintained by intercellular interactions and signaling networks of a plant peptide hormone, Root Meristem Growth Factor 1 (RGF1). How RGF1 regulates root meristem development is an essential question to understand stem cell function. Although five receptors of RGF1 have recently been identified, the downstream signaling mechanism remains unknown. Here, we report a series of signaling events following RGF1 action. The RGF1-receptor pathway controls distribution of reactive oxygen species (ROS) along the developmental zones of the Arabidopsis root. We identify a novel transcription factor, RGF1 INDUCIBLE TRANSCRIPTION FACTOR 1 (RITF1), which plays a central role in mediating RGF1 signaling. Manipulating RITF1 expression leads to redistribution of ROS along the root developmental zones. Changes in ROS distribution, in turn, enhance the stability of the PLETHORA2 (PLT2) protein, a master regulator of root stem cells. Taken together, our study clearly depicts a signaling cascade initiated by RGF1 and links the RGF1 peptide to ROS regulatory mechanisms.

scholarly journals Proteome and Secretome Dynamics of Stem Cell-Derived Retinal Pigmented Epithelium in Response to Acute and Chronic ROS

2019 ◽  
Author(s):  
Jesse G. Meyer ◽  
Thelma Garcia ◽  
Birgit Schilling ◽  
Bradford W. Gibson ◽  
Deepak A. Lamba
Keyword(s):  
Reactive Oxygen Species ◽  
Stem Cell ◽  
Molecular Mechanisms ◽  
Reactive Oxygen ◽  
Age Related Macular Degeneration ◽  
Oxygen Species ◽  
Urotensin Ii ◽  
Retinal Pigmented Epithelium ◽  
Age Related ◽  
Pigmented Epithelium

AbstractAge-related macular degeneration (AMD) is the leading cause of blindness in developed countries, and is characterized by slow retinal degeneration linked to chronic oxidative stress in the retinal pigmented epithelium (RPE). The exact molecular mechanisms that lead to RPE death and dysfunction in response to chronic reactive oxygen species (ROS) are still unclear. In this work, human stem cell-derived RPE samples were treated with a low dose of paraquat (PQ) for 1 week or 3 weeks to induce chronic reactive oxygen species (ROS) stress. Cells were then harvested and both the intracellular and secreted RPE proteomes were quantified by mass spectrometry. Inside the RPE, chronic ROS caused concerted increase of glycolytic proteins but decreased mitochondrial proteins, as well as decreased extracellular matrix proteins and membrane proteins required for endocytosis. From the secreted proteins, we found that stressed RPE secrete over 1,000 detectable proteins, and the composition of the proteins secreted from RPE changes due to chronic ROS. Notably, secreted APOE is decreased 4-fold due to 3 weeks of chronic ROS stress, and urotensin-II, the strongest known vasoconstrictor, doubles. Further, secreted TGF-beta is increased, and its cognate signaler BMP1 decreased in the secretome. Together, these alterations of the RPE proteome and protein secretome paint a detailed molecular picture of the retinal stress response in space and time.

scholarly journals Structural & biochemical study of peroxiredoxin from Candidatus liberibacter asiaticus

2014 ◽  
Vol 70 (a1) ◽  
pp. C840-C840
Author(s):  
Anamika Singh ◽  
Pravindra Kumar ◽  
Ashwani Kumar Sharma
Keyword(s):  
Reactive Oxygen Species ◽  
Peroxidase Activity ◽  
Reactive Oxygen ◽  
Site Directed Mutagenesis ◽  
Mutant Form ◽  
Oxygen Species ◽  
Candidatus Liberibacter Asiaticus ◽  
Candidatus Liberibacter ◽  
Antioxidant Proteins ◽  
Liberibacter Asiaticus

Candidatus Liberibacter asiaticus is a fastidious prokaryotic α-proteobacterium that has not been cultured as yet. It is the causative agent of Citrus Huanglongbing (HLB) is a disease that infects citrus plants causing huge losses to the citrus industry. The plants employ a variety of defense mechanisms to combat the pathogen attack which mainly involves the formation of reactive oxygen species (ROS) mainly hydrogen peroxide, peroxynitrite and organic hydroperoxides. The microbe on the other hand has antioxidant proteins that counter the reactive oxygen species. Peroxiredoxin (Prx) which is known to play a crucial role in peroxide detoxification is a super family of one of such antioxidant proteins. In this work, we have determined the crystal structure of Bacterioferritin Comigratory Protein (BCP) that belongs to 1-Cys Prx enzyme having peroxidatic/sulfenic acid cysteine (C-47) but lacks the resolving cysteine. Furthermore, we are investigating structural aspects to gain insight into the binding of different peroxide substrates at the active site. Site directed mutagenesis has been done to introduce the non-conserved resolving cysteine to study its effect on peroxidase activity in both wild type and mutant form. Biochemically, both BCP 1-cys and BCP 2-cys have shown peroxidase activity. DNA protection activity against oxidative damage and cell-line based assays has implicated its protective role towards the hypoxic condition of cells. Hence, the protein becomes a lucrative target for ligands which might be potential antimicrobials.

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