Ultrastructure of spherites in the midgut diverticula and Malpighian tubules of the harvestman Amilenus aurantiacus during the winter diapause

1971 ◽

Vol 29 ◽

pp. 402-403

Author(s):

Brendan Clifford

Keyword(s):

Fine Structure ◽

Culex Pipiens ◽

Stellate Cell ◽

Malpighian Tubule ◽

Cell Types ◽

Instar Larva ◽

Malpighian Tubules ◽

Calliphora Erythrocephala ◽

Distinct Cell ◽

Basal Plasma

An ultrastructural investigation of the Malpighian tubules of the fourth instar larva of Culex pipiens was undertaken as part of a continuing study of the fine structure of transport epithelia.Each of the five Malpighian tubules was found to be morphologically identical and regionally undifferentiated. Two distinct cell types, the primary and stellate, were found intermingled along the length of each tubule. The ultrastructure of the stellate cell was previously described in the Malpighian tubule of the blowfly, Calliphora erythrocephala by Berridge and Oschman.The basal plasma membrane of the primary cell is extremely irregular, giving rise to a complex interconnecting network of basal channels. The compartments of cytoplasm entrapped within this system of basal infoldings contain mitochondria, free ribosomes, and small amounts of rough endoplasmic reticulum. The mitochondria are distinctive in that the cristae run parallel to the long axis of the organelle.

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Ultrastructural Characterization of Malpighian Tubules of Rhodnius prolixus During the Insect's Feeding Cycle and Under Hyperosmotic Shock

Entomological News ◽

10.3157/021.129.0208 ◽

2020 ◽

Vol 129 (2) ◽

pp. 189

Author(s):

Jacenir Reis dos Santos-Mallet ◽

Simone Patrícia Carneiro Freitas ◽

Maria Luiza Ribeiro de Oliveira ◽

Alice Helena Ricardo-Silva ◽

Aníbal Gil Lopes ◽

...

Keyword(s):

Rhodnius Prolixus ◽

Malpighian Tubules ◽

Hyperosmotic Shock ◽

Ultrastructural Characterization ◽

Feeding Cycle

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Faculty Opinions recommendation of The adult Drosophila malpighian tubules are maintained by multipotent stem cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1089501.544753 ◽

2007 ◽

Author(s):

Yukiko Yamashita

Keyword(s):

Stem Cells ◽

Malpighian Tubules ◽

Multipotent Stem Cells ◽

Adult Drosophila

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Progression of Watermelon Bud Necrosis Virus Infection in Its Vector, Thrips palmi

Cells ◽

10.3390/cells10020392 ◽

2021 ◽

Vol 10 (2) ◽

pp. 392

Author(s):

Amalendu Ghosh ◽

Priti ◽

Bikash Mandal ◽

Ralf G. Dietzgen

Keyword(s):

Virus Infection ◽

Salivary Glands ◽

Frankliniella Occidentalis ◽

Western Flower Thrips ◽

Malpighian Tubules ◽

Necrosis Virus ◽

Posterior Portion ◽

Thrips Palmi ◽

Thrips Species ◽

Anterior Midgut

Thrips are important pests of agricultural, horticultural, and forest crops worldwide. In addition to direct damages caused by feeding, several thrips species can transmit diverse tospoviruses. The present understanding of thrips–tospovirus relationships is largely based on studies of tomato spotted wilt virus (TSWV) and Western flower thrips (Frankliniella occidentalis). Little is known about other predominant tospoviruses and their thrips vectors. In this study, we report the progression of watermelon bud necrosis virus (WBNV) infection in its vector, melon thrips (Thrips palmi). Virus infection was visualized in different life stages of thrips using WBNV-nucleocapsid protein antibodies detected with FITC-conjugated secondary antibodies. The anterior midgut was the first to be infected with WBNV in the first instar larvae. The midgut of T. palmi was connected to the principal salivary glands (PSG) via ligaments and the tubular salivary glands (TSG). The infection progressed to the PSG primarily through the connecting ligaments during early larval instars. The TSG may also have an ancillary role in disseminating WBNV from the midgut to PSG in older instars of T. palmi. Infection of WBNV was also spread to the Malpighian tubules, hindgut, and posterior portion of the foregut during the adult stage. Maximum virus-specific fluorescence in the anterior midgut and PSG indicated the primary sites for WBNV replication. These findings will help to better understand the thrips–tospovirus molecular relationships and identify novel potential targets for their management. To our knowledge, this is the first report of the WBNV dissemination path in its vector, T. palmi.

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Malpighian tubules in harvestmen

PROTOPLASMA ◽

10.1007/s00709-021-01634-0 ◽

2021 ◽

Author(s):

Saška Lipovšek ◽

Peter Kozel ◽

Gerd Leitinger ◽

Tone Novak

Keyword(s):

Malpighian Tubules

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The Genes raw and ribbon Are Required for Proper Shape of Tubular Epithelial Tissues in Drosophila

Genetics ◽

10.1093/genetics/147.1.243 ◽

1997 ◽

Vol 147 (1) ◽

pp. 243-253 ◽

Cited By ~ 1

Author(s):

Joseph Jack ◽

Guy Myette

Keyword(s):

Embryonic Development ◽

Salivary Glands ◽

Cell Shape ◽

Malpighian Tubules ◽

Dorsal Closure ◽

Epithelial Tissues ◽

Cell Rearrangement

Abstract The products of two genes, raw and ribbon (rib), are required for the proper morphogenesis of a variety of tissues. Malpighian tubules mutant for raw or rib are wider and shorter than normal tubules, which are only two cells in circumference when they are fully formed. The mutations alter the shape of the tubules beginning early in their formation and block cell rearrangement late in development, which normally lengthens and narrows the tubes. Mutations of both genes affect a number of other tissues as well. Both genes are required for dorsal closure and retraction of the CNS during embryonic development. In addition, rib mutations block head involution, and broaden and shorten other tubular epithelia (salivary glands, tracheae, and hindgut) in much same manner as they alter the shape of the Malpighian tubules. In tissues in which the shape of cells can be observed readily, rib mutations alter cell shape, which probably causes the change in shape of the organs that are affected. In double mutants raw enhances the phenotypes of all the tissues that are affected by rib but unaffected by raw alone, indicating that raw is also active in these tissues.

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Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules

AJP Cell Physiology ◽

10.1152/ajpcell.1991.261.3.c521 ◽

1991 ◽

Vol 261 (3) ◽

pp. C521-C529 ◽

Cited By ~ 66

Author(s):

J. L. Hegarty ◽

B. Zhang ◽

T. L. Pannabecker ◽

D. H. Petzel ◽

M. D. Baustian ◽

...

Keyword(s):

Yellow Fever ◽

Apical Membrane ◽

Basolateral Membrane ◽

Fluid Secretion ◽

Ringer Solution ◽

Malpighian Tubules ◽

Membrane Voltage ◽

Dibutyryl Camp ◽

K Secretion ◽

Transepithelial Voltage

The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10(-4) M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (VT) and depolarized the basolateral membrane voltage (Vbl) with no effect on apical membrane voltage (Va). Total transepithelial resistance (RT) and the fractional resistance of the basolateral membrane (fRbl) significantly decreased. Bumetanide also hyperpolarized VT and depolarized Vbl, however without significantly affecting RT and fRbl. Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.

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