AbstractObjectivesGheeis widely considered as the Indian name for clarified butterfat and processing ofgheewith therapeutic herbs i.e.,ghritais renowned for augmenting their medicinal properties. The wound is considered as a challenging clinical problem with early and late complications. To reduce the burden of wounds with the shortest period and minimum scaring, an attempt was made to prepare and evaluate the wound healing potential ofgheebased polyherbal formulation.MethodsBased on local ethnic tribal claims,Semecarpus anacardiumL.,Argemone mexicanaL.,Cocculus hirsutusL., andWoodfordia fruticosaK. were collected from Western Ghats of India. The polyherbalBhallatakadi Ghrita(BG) formulation was prepared as perAyurvedicprocedure and assessed for its wound healing potential using incision and excision wound animal models.ResultsBG treated group showed a complete contraction of wounds (99.82 ± 0.10%) (p<0.001) with 15.17 ± 0.40 days re-epithelization time and breaking strength (531.50 ± 5.89) (p<0.05). The hydroxyproline content of BG was found to be significantly higher i.e., 4.23 ± 0.21 (p<0.05). Quantitative estimation of BG exhibited 54.7 ± 3.7 mg100 g−1of polyphenols and 42.3 ± 5.4 mg.100 g−1flavonoids in terms of gallic acid and quercetin respectively. HPLC analysis revealed the presence of gallic acid and quercetin whereas the presence of fatty acids was confirmed by GC-MS analysis.ConclusionsIt may conclude that the presence of quercetin, gallic acid, and fatty acids could have accelerated the healing rate of theghritaformulation, as they have already been known for their potential wound healing properties.
Biogenic synthesis of gold nanoparticles using Argemone mexicana L. and their cytotoxic and genotoxic effects on human colon cancer cell line (HCT-15)
