TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

2018 ◽  
Vol 41 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Felipe Vázquez-Flota ◽  
Jorge Rubio-Piña ◽  
Jorge Xool-Tamayo ◽  
Mariela Vergara-Olivares ◽  
Yahaira Tamayo-Ordoñez ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Transport Mechanism ◽  
Gene Products ◽  
Argemone Mexicana ◽  
Benzylisoquinoline Alkaloid ◽  
Stems And Leaves ◽  
Specific Reactions

The distribution of berberine and sanguinarine was analyzed in roots, stems and leaves of mature Argemone mexicana plants, along with that of transcripts corresponding to selected genes involved in both early biosynthetic reactions, which are common to both alkaloids, and in the late specific reactions conducting to the formation of each of them. Roots were the main sites of alkaloid accumulation, though they showed the lowest accumulation of the analyzed transcripts. Results are discussed in terms of the operation of a possible transport mechanism of alkaloids between the aerial tissues and the roots, or the occurrence of different biosynthetic alternative reactions in both parts, aerial and underground tissues, involving different gene products, yet with similar catalytic capacities.

scholarly journals Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2226
Author(s):  
Jorge Xool-Tamayo ◽  
Yahaira Tamayo-Ordoñez ◽  
Miriam Monforte-González ◽  
José Armando Muñoz-Sánchez ◽  
Felipe Vázquez-Flota
Keyword(s):  
Gene Expression ◽  
Tissue Distribution ◽  
Early Development ◽  
Transcriptional Activity ◽  
Seedling Development ◽  
Transport Systems ◽  
Alkaloid Biosynthesis ◽  
Argemone Mexicana ◽  
Early Stages ◽  
Low Levels

The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems.

scholarly journals Tissue Distribution of Murine Muc19/Smgc Gene Products

2009 ◽  
Vol 58 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Biswadip Das ◽  
Melanie N. Cash ◽  
Arthur R. Hand ◽  
Armin Shivazad ◽  
Scott S. Grieshaber ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Gene Products

scholarly journals Tissue Distribution of the Four Gene Products of the Plasma Membrane Ca Pump

1995 ◽  
Vol 270 (20) ◽  
pp. 12184-12190 ◽  
Author(s):  
Thomas P. Stauffer ◽  
Danilo Guerini ◽  
Ernesto Carafoli
Keyword(s):  
Plasma Membrane ◽  
Tissue Distribution ◽  
Gene Products ◽  
Ca Pump

scholarly journals In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis

2020 ◽  
Vol 58 (2) ◽  
pp. 135-145 ◽  
Author(s):  
Joel Horacio Elizondo-Luevano ◽  
Julia Verde-Star ◽  
Azucena González-Horta ◽  
Rocío Castro-Ríos ◽  
Magda Elizabeth Hernández-García ◽  
...  
Keyword(s):  
Trichomonas Vaginalis ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Argemone Mexicana ◽  
Antiparasitic Activity ◽  
Sexually Transmitted ◽  
Methanolic Extracts ◽  
Stems And Leaves ◽  
Vitro Effect

Infections caused by <i>Trichomonas vaginalis</i> in humans are one of the main public health problems caused by sexually transmitted diseases. Objective of this study was to evaluate potential biological activity of the medicinal plant <i>Argemone mexicana</i> (Mexican poppy) on <i>T. vaginalis</i>. Methanolic extracts of the stems and leaves of <i>A. mexicana</i>, and different fractions were prepared with solvents of different polarities. The extracts and functional groups were detected containing sterols, triterpenes, quinones, flavonoids and, alkaloids. Extracts from both the stems and leaves of <i>A. mexicana</i> inhibited the growth of <i>T. vaginalis</i> with half-maximal inhibitory concentration value of 70.6 and 67.2 μg/ml, respectively. In the active fractions, the most abundant compounds were berberine and jatrorrhizine, with presumed antiparasitic activity.

Effects of Vincristine on Endothelial Microtubules in the Spinal Cord

1976 ◽  
Vol 34 ◽  
pp. 58-59
Author(s):  
John L. Beggs ◽  
John D. Waggener ◽  
Wanda Miller
Keyword(s):  
Spinal Cord ◽  
Biological Activity ◽  
Horseradish Peroxidase ◽  
Transport Mechanism ◽  
Vasogenic Edema ◽  
Vesicular Transport ◽  
Close Proximity

Microtubules (MT) are versatile organelles participating in a wide variety of biological activity. MT involvement in the movement and transport of cytoplasmic components has been well documented. In the course of our study on trauma-induced vasogenic edema in the spinal cord we have concluded that endothelial vesicles contribute to the edema process. Using horseradish peroxidase as a vascular tracer, labeled endothelial vesicles were present in all situations expected if a vesicular transport mechanism was in operation. Frequently,labeled vesicles coalesced to form channels that appeared to traverse the endothelium. The presence of MT in close proximity to labeled vesicles sugg ested that MT may play a role in vesicular activity.

A microscopic marker experiment study on high temperature oxidation of Ni-Al alloy

1986 ◽  
Vol 44 ◽  
pp. 514-515
Author(s):  
Shou-kong Fan
Keyword(s):  
Transport Mechanism ◽  
High Temperature Oxidation ◽  
Transverse Section ◽  
Al Alloy ◽  
Metal Interface ◽  
Electron Microscopic ◽  
Temperature Oxidation ◽  
Analytical Electron ◽  
Microscopic Studies ◽  
First Time

Transmission and analytical electron microscopic studies of scale microstructures and microscopic marker experiments have been carried out in order to determine the transport mechanism in the oxidation of Ni-Al alloy. According to the classical theory, the oxidation of nickel takes place by transport of Ni cations across the scale forming new oxide at the scale/gas interface. Any markers deposited on the Ni surface are expected to remain at the scale/metal interface after oxidation. This investigation using TEM transverse section techniques and deposited microscopic markers shows a different result,which indicates that a considerable amount of oxygen was transported inward. This is the first time that such fine-scale markers have been coupled with high resolution characterization instruments such as TEM/STEM to provide detailed information about evolution of oxide scale microstructure.

Identification of a baculovirus polyhedron formation mutant with a novel phenotype

1996 ◽  
Vol 54 ◽  
pp. 796-797
Author(s):  
James M. Slavicek ◽  
Melissa J. Mercer ◽  
Mary Ellen Kelly
Keyword(s):  
Viral Gene ◽  
Gene Products ◽  
Type Number ◽  
Infection Cycle ◽  
Wild Type ◽  
Protein Matrix ◽  
Insect Midgut ◽  
Viral Particles ◽  
Budded Virus ◽  
Viral Form

Nucleopolyhedroviruses (NPV, family Baculoviridae) produce two morphological forms, a budded virus form and a viral form that is occluded into a paracrystalline protein matrix. This structure is termed a polyhedron and is composed primarily of the protein polyhedrin. Insects are infected by NPVs after ingestion of the polyhedron and release of the occluded virions through dissolution of the polyhedron in the alkaline environment of the insect midgut. Early after infection the budded virus form is produced. It buds through the plasma membrane and then infects other cells. Later in the infection cycle the occluded form of the virus is generated (reviewed by Blissard and Rohrmann, 1990).The processes of polyhedron formation and virion occlusion are likely to involve a number of viral gene products. However, only two genes, the polyhedrin gene and 25K FP gene, have been identified to date that are necessary for the wild type number of polyhedra to be formed and viral particles occluded.

New insights into heparan sulphate biosynthesis from the study of mutant mice

2002 ◽  
Vol 69 ◽  
pp. 47-57 ◽  
Author(s):  
Catherine L. R. Merry ◽  
John T. Gallagher
Keyword(s):  
Growth Factors ◽  
Biosynthetic Pathway ◽  
Heparan Sulphate ◽  
Mutant Mice ◽  
Gene Products ◽  
Multiple Gene ◽  
Adhesion Proteins ◽  
Ligand Interactions

Heparan sulphate (HS) is an essential co-receptor for a number of growth factors, morphogens and adhesion proteins. The biosynthetic modifications involved in the generation of a mature HS chain may determine the strength and outcome of HS–ligand interactions. These modifications are catalysed by a complex family of enzymes, some of which occur as multiple gene products. Various mutant mice have now been generated, which lack the function of isolated components of the HS biosynthetic pathway. In this discussion, we outline the key findings of these studies, and use them to put into context our own work concerning the structure of the HS generated by the Hs2st-/- mice.

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