Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with improved stability and selective toxicity to bacteria over mammalian cells

2017 ◽  
Vol 207 (1) ◽  
pp. 39-53 ◽  
Author(s):  
Swati Jaiswal ◽  
Prashant Mishra
Keyword(s):  
Silver Nanoparticles ◽  
Mammalian Cells ◽  
Antibiofilm Activity ◽  
Selective Toxicity ◽  
Improved Stability

scholarly journals Silver Nanoparticles Induce Neutrophil Extracellular Traps Via Activation of PAD and Neutrophil Elastase

Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 317
Author(s):  
HanGoo Kang ◽  
Jinwon Seo ◽  
Eun-Jeong Yang ◽  
In-Hong Choi
Keyword(s):  
Silver Nanoparticles ◽  
Neutrophil Elastase ◽  
Mammalian Cells ◽  
Antimicrobial Properties ◽  
Neutrophil Extracellular Traps ◽  
Arginine Deiminase ◽  
Human Neutrophils ◽  
Extracellular Traps ◽  
Peptidyl Arginine Deiminase ◽  
Key Factor

Silver nanoparticles (AgNPs) are widely used in various fields because of their antimicrobial properties. However, many studies have reported that AgNPs can be harmful to both microorganisms and humans. Reactive oxygen species (ROS) are a key factor of cytotoxicity of AgNPs in mammalian cells and an important factor in the immune reaction of neutrophils. The immune reactions of neutrophils include the expulsion of webs of DNA surrounded by histones and granular proteins. These webs of DNA are termed neutrophil extracellular traps (NETs). NETs allow neutrophils to catch and destroy pathogens in extracellular spaces. In this study, we investigated how AgNPs stimulate neutrophils, specifically focusing on NETs. Freshly isolated human neutrophils were treated with 5 or 100 nm AgNPs. The 5 nm AgNPs induced NET formation, but the 100 nm AgNPs did not. Subsequently, we investigated the mechanism of AgNP-induced NETs using known inhibitors related to NET formation. AgNP-induced NETs were dependent on ROS, peptidyl arginine deiminase, and neutrophil elastase. The result in this study indicates that treatment of 5 nm AgNPs induce NET formation through histone citrullination by peptidyl arginine deiminase and histone cleavage by neutrophil elastase.

scholarly journals Enhanced Genotoxicity of Silver Nanoparticles in DNA Repair Deficient Mammalian Cells

2012 ◽  
Vol 3 ◽  
Author(s):  
Hui Kheng Lim ◽  
P. V. Asharani ◽  
M. Prakash Hande
Keyword(s):  
Silver Nanoparticles ◽  
Dna Repair ◽  
Mammalian Cells

scholarly journals Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials

Chemistry ◽  
2021 ◽  
Vol 3 (4) ◽  
pp. 1271-1285
Author(s):  
Patricia Zimet ◽  
Ruby Valadez ◽  
Sofía Raffaelli ◽  
María Belén Estevez ◽  
Helena Pardo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Value Added ◽  
Green Technology ◽  
Natural Food ◽  
Antibiofilm Activity ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Biogenic Silver Nanoparticles

Microbial technology offers a green alternative for the synthesis of value-added nanomaterials. In particular, fungal compounds can improve silver nanoparticle production, stabilizing colloidal nanoparticles. Based on a previous study by our group, silver nanoparticles obtained using the extracellular cell-free extracts of Phanerochaete chrysosporium (PchNPs) have shown antimicrobial and antibiofilm activity against Gram-negative bacteria. Moreover, nisin—a bacteriocin widely used as a natural food preservative—has recently gained much attention due its antimicrobial action against Gram-positive bacteria in biomedical applications. Therefore, the aim of this work was to conjugate biogenic silver nanoparticles (PchNPs) with nisin to obtain nanoconjugates (PchNPs@nis) with enhanced antimicrobial properties. Characterization assays were conducted to determine physicochemical properties of PchNPs@nis, and also their antibacterial and antibiofilm activities were studied. The formation of PchNPs@nis was confirmed by UV-Vis, TEM, and Raman spectroscopy analysis. Different PchNPs@nis nanobioconjugates showed diameter values in the range of 60–130 nm by DLS and surface charge values between −20 and −13 mV. Nisin showed an excellent affinity to PchNPs, with binding efficiencies higher than 75%. Stable synthesized PchNPs@nis nanobioconjugates were not only able to inhibit biofilm formation by S. aureus, but also showed inhibition of the planktonic cell growth of Staphyloccocus aureus and Escherichia coli, broadening the spectrum of action of the unconjugated antimicrobials against Gram-positive and Gram-negative bacteria. In conclusion, these results show the promising application of PchNPs@nis, prepared via green technology, as potential antimicrobial nanomaterials.

Silver Nanoparticles

2020 ◽  
pp. 432-449
Author(s):  
Boguslaw Buszewski ◽  
Viorica Railean Plugaru ◽  
Pawel Pomastowski ◽  
Anatoli Sidorenco
Keyword(s):  
Silver Nanoparticles ◽  
Growth And Development ◽  
Antimicrobial Properties ◽  
Future Application ◽  
Synthesis Methods ◽  
Selective Toxicity ◽  
Gram Negative ◽  
Cancerous Cells ◽  
Nano Size ◽  
Intense Research

Silver nanoparticles (AgNPs), as a material with attractive properties, has received interest worldwide by the implementation of synthesis particles at nano-size. AgNPs has triggered intense research on the production of nanoparticles using different synthesis methods due actual and future application. The increasing attention of AgNPs resulted in the growth and development of nanoparticles synthesis methods. Recently, various approaches, including chemical and biological, have been used for them preparation. In order to obtain information about size, shape, or optical properties of nanoparticles, a broad range of analytical methods—microscopy, spectroscopy, and spectrometry techniques—have been used. AgNPs exhibit attractive antimicrobial properties against bacteria (Gram-positive and Gram-negative), fungi, and show also selective toxicity toward normal and cancerous cells. This chapter reviewed the methods of AgNPs synthesis, their characteristics, antimicrobial activity, and cytotoxicity against normal and tumor cells.

scholarly journals Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant Klebsiella pneumoniae

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Muhammad Hussnain Siddique ◽  
Bilal Aslam ◽  
Muhammad Imran ◽  
Asma Ashraf ◽  
Habibullah Nadeem ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biofilm Formation ◽  
Extracellular Polymeric Substances ◽  
Microtiter Plate ◽  
Cellular Protein ◽  
Antibiofilm Activity ◽  
Biofilm Inhibition ◽  
Microtiter Plate Assay ◽  
Protein Leakage ◽  
Hela Cell Lines

Antibiotic resistance against present antibiotics is rising at an alarming rate with need for discovery of advanced methods to treat infections caused by resistant pathogens. Silver nanoparticles are known to exhibit satisfactory antibacterial and antibiofilm activity against different pathogens. In the present study, the AgNPs were synthesized chemically and characterized by UV-Visible spectroscopy, scanning electron microscopy, and X-ray diffraction. Antibacterial activity against MDR K. pneumoniae strains was evaluated by agar diffusion and broth microdilution assay. Cellular protein leakage was determined by the Bradford assay. The effect of AgNPs on production on extracellular polymeric substances was evaluated. Biofilm formation was assessed by tube method qualitatively and quantitatively by the microtiter plate assay. The cytotoxic potential of AgNPs on HeLa cell lines was also determined. AgNPs exhibited an MIC of 62.5 and 125 μg/ml, while their MBC is 250 and 500 μg/ml. The production of extracellular polymeric substance decreased after AgNP treatment while cellular protein leakage increased due to higher rates of cellular membrane disruption by AgNPs. The percentage biofilm inhibition was evaluated to be 64% for K. pneumoniae strain MF953600 and 86% for MF953599 at AgNP concentration of 100 μg/ml. AgNPs were evaluated to be minimally cytotoxic and safe at concentrations of 15-120 μg/ml. The data evaluated by this study provided evidence of AgNPs being safe antibacterial and antibiofilm compounds against MDR K. pneumoniae.

Carbon-bonded silver nanoparticles: alkyne-functionalized ligands for SERS imaging of mammalian cells

2011 ◽  
Vol 47 (11) ◽  
pp. 3156 ◽  
Author(s):  
David C. Kennedy ◽  
Craig S. McKay ◽  
Li-lin Tay ◽  
Yanouchka Rouleau ◽  
John P. Pezacki
Keyword(s):  
Silver Nanoparticles ◽  
Mammalian Cells ◽  
Sers Imaging ◽  
Functionalized Ligands

Antibiofilm activity of synthesized electrospun core-shell nanofiber composites of PLA and PVA with silver nanoparticles

2018 ◽  
Vol 5 (9) ◽  
pp. 095001 ◽  
Author(s):  
Hamad F Alharbi ◽  
Monis Luqman ◽  
Shams Tabrez Khan
Keyword(s):  
Silver Nanoparticles ◽  
Core Shell ◽  
Antibiofilm Activity
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