Lousicidal activity of synthesized silver nanoparticles using Lawsonia inermis leaf aqueous extract against Pediculus humanus capitis and Bovicola ovis

In this study, an attempt was made to synthesize silver nanoparticles (Ag-NPs) using Grewia optiva leaf extract and isolated compounds. The bioreductant capacity of Grewia optiva leaf extract for the synthesis of Ag-NPs was assessed using various confirmatory techniques like thermogravimetric analysis (TGA), particle size analysis (PSA), energy-dispersive X-ray (EDX), X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and UV-Visible spectroscopy. The presence of various bioactive compounds in leaf aqueous extract was confirmed through HPLC analysis, and 8 compounds were identified among the different peaks present in the chromatogram. Biopotencies like antioxidant, antibacterial, and effect on hair growth were determined for extract and NPs. Antioxidant capacities were assessed through standard ABTS and DPPH methods. The antibacterial potential was evaluated in terms of zone of inhibition, minimum bactericidal concentration, and minimum inhibitory concentration of the Ag-NPs and the leaf extract against selected strains of bacteria, whereas the effect on growth of rabbit hair was studied through topical treatment for a specific period of time. Better antibacterial and DPPH and ABTS free radical inhibition was observed for the formulated Ag-NPs as compared to leaf extract. The previously isolated eight compounds from this plant’s chloroform and ethyl acetate extracts were also tested for their bioreductant capacities. Out of them, the highest amount of precipitates was obtained with compound VII ((2,5-dihydroxyphenyl)-3 ′ ,6 ′ ,8 ′ -trihydroxyl-4H-chromen-4 ′ -one). The study implies that the biogenically engineered nanoscale particles could have promising biological activities in comparison to parental extract and they need to be investigated further as potential therapeutic agents to be used as antibacterial and antioxidant agents and for hair growth enhancement.

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Evaluation of leaf aqueous extract and synthesized silver nanoparticles using Nerium oleander against Anopheles stephensi (Diptera: Culicidae)

Parasitology Research ◽

10.1007/s00436-012-3220-3 ◽

2012 ◽

Vol 112 (3) ◽

pp. 981-990 ◽

Cited By ~ 60

Author(s):

Mathath Roni ◽

Kadarkarai Murugan ◽

Chellasamy Panneerselvam ◽

Jayapal Subramaniam ◽

Jiang-Shiou Hwang

Keyword(s):

Silver Nanoparticles ◽

Aqueous Extract ◽

Anopheles Stephensi ◽

Nerium Oleander ◽

Leaf Aqueous Extract

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GREEN SYNTHESIS OF SILVER NANOPARTICLES USING GRAVIOLA LEAF AQUEOUS EXTRACT AT ROOM TEMPERATURE

Kongunadu Research Journal ◽

10.26524/krj86 ◽

2015 ◽

Vol 2 (2) ◽

pp. 6-10

Author(s):

Ranjithkumar R ◽

Chandar Shekar B ◽

Senthil Kumaran C.K ◽

Sharmila C ◽

Simi V

Keyword(s):

Silver Nanoparticles ◽

Infrared Spectroscopy ◽

Aqueous Extract ◽

Sem Analysis ◽

X Ray Diffraction ◽

Electron Microscopic ◽

Green Route ◽

Leaf Aqueous Extract ◽

Fourier Transformation Infrared Spectroscopy ◽

Ft Ir

Improvement of green route for the synthesis of silver nanoparticles with plant extracts plays a very important role in nanotechnology without any harmful chemicals. The present investigation demonstrates the synthesis of silver nanoparticles by treating silver nitrate with Graviola leaf extract at room temperature.The effect of the extract on the formation of silver nanoparticles was characterized by UV-Vis spectrum, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and Scanning electron microscopic (SEM) analysis. The UV-Vis spectra results show a strong resonance centered on the surface of silvernanoparticles (AgNPs) at 400-450 nm. The Fourier transformation infrared spectroscopy spectral study demonstrates Graviola leaf aqueous extract acted as the reducing and stabilizing agent during the synthesis of silver nanoparticles. XRD and SEM studies revealed that the synthesized silver nanoparticles shows spherical in shape with average particles size around 30-70 nm.

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Novel Curcumae Kwangsiensis mediated synthesis of silver nanoparticles for anti-lung cancer activity: a preclinical trial study

Archives of Medical Science ◽

10.5114/aoms/134059 ◽

2021 ◽

Author(s):

Zhihong Liu ◽

Zhuohong Zhang ◽

Xiaomei Du ◽

Ying Liu ◽

Abdullah Alarfaj ◽

...

Keyword(s):

Lung Cancer ◽

Silver Nanoparticles ◽

Cell Lines ◽

Cancer Cell ◽

Aqueous Extract ◽

Ag Nanoparticles ◽

Human Lung ◽

Human Lung Cancer ◽

Lung Cancer Cell ◽

Leaf Aqueous Extract

IntroductionThe present work indicated the green synthesis and characterization and cytotoxicity, antioxidant, and anti-human lung cancer activities of silver nanoparticles containing Curcumae Kwangsiensis Folium leaf aqueous extract.Material and methodsAg nanoparticles have been produced by mixing the AgNO3 solution with aqueous Curcumae Kwangsiensis Folium leaf extract. Characterization of Ag nanoparticles was done by FE‐SEM, FT‐IR, TEM, and UV-Vis. FE-SEM and TEM images revealed an average diameter of 15-21 nm for the nanoparticles. MTT assay was used on common human lung cancer cell lines i.e., lung well-differentiated bronchogenic adenocarcinoma (HLC-1), lung moderately differentiated adenocarcinoma (LC-2/ad), and lung poorly differentiated adenocarcinoma (PC-14) cell lines to survey the cytotoxicity and anti-human lung cancer effects of Ag nanoparticles.ResultsThey had very low cell viability and high anti-human lung cancer activities dose-dependently against HLC-1, LC-2/ad, and PC-14 cell lines without any cytotoxicity on the normal cell line (HUVEC). The IC50 of Ag nanoparticles were 249, 187, and 152 µg/mL against HLC-1, LC-2/ad, and PC-14 cell lines, respectively. The best results of cytotoxicity and anti-human lung cancer properties were seen in the concentration of 1000 µg/mL. Ag nanoparticles inhibited half of the DPPH molecules in the concentration of 135 µg/mL.ConclusionsMaybe significant anti-human lung cancer potentials of Ag nanoparticles synthesized by Curcumae Kwangsiensis Folium leaf aqueous extract against common human lung cancer cell lines are linked to their antioxidant activities. After confirming the above results in the clinical trial researches, this formulation can be administrated to treat human lung cancers in humans.

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