scholarly journals Unnoticed arrival of two dipteran species in Austria: the synanthropic moth fly Clogmia albipunctata (Williston, 1893) and the parasitic bird louse fly Ornithoica turdi (Olivier in Latreille, 1811)

2019 ◽  
Vol 119 (2) ◽  
pp. 737-740 ◽  
Author(s):  
Carina Zittra ◽  
Ellen R. Schoener ◽  
Rüdiger Wagner ◽  
Mike Heddergott ◽  
Georg G. Duscher ◽  
...  
Keyword(s):  
Monitoring Program ◽  
Species Level ◽  
Reference Sequence ◽  
Single Specimen ◽  
Veterinary Importance ◽  
Clogmia Albipunctata ◽  
Dipteran Species ◽  
Mosquito Monitoring

AbstractIn the framework of a mosquito-monitoring program conducted from 2014 to 2018, non-culicid dipteran bycatch was identified to species-level with a focus on Diptera of medical and veterinary importance as part of a biodiversity initiative and barcoding project (“Austrian Barcode of Life”). Two species hitherto not known from Austria, the regularly sampled synanthropic moth fly Clogmia albipunctata (Psychodidae) and a single specimen of the louse fly Ornithoica turdi (Hippoboscidae), were collected in Vienna and Lower Austria. We confirmed identification results using a barcoding approach and provide the first reference sequence for O. turdi.

Mosquito-only flaviviruses, isolated from Aedes albopictus in Slovenia: results of a pilot mosquito monitoring program

Biologia ◽  
2018 ◽  
Vol 73 (12) ◽  
pp. 1277-1282 ◽  
Author(s):  
Katja Kalan ◽  
Tatjana Avšič-Županc ◽  
Katarina Resman Rus ◽  
Elena Bužan ◽  
Darja Duh
Keyword(s):  
Aedes Albopictus ◽  
Monitoring Program ◽  
Mosquito Monitoring

scholarly journals The InBIO Barcoding Initiative Database: DNA barcodes of Portuguese Diptera 01

2020 ◽  
Vol 8 ◽  
Author(s):  
Sonia Ferreira ◽  
Rui Andrade ◽  
Ana Gonçalves ◽  
Pedro Sousa ◽  
Joana Paupério ◽  
...  
Keyword(s):  
Species Level ◽  
Distribution Data ◽  
Dna Barcodes ◽  
Online Database ◽  
Global Biodiversity Information Facility ◽  
Life Data ◽  
Tagus River ◽  
Global Biodiversity ◽  
Biodiversity Information ◽  
Dipteran Species

The InBIO Barcoding Initiative (IBI) Diptera 01 dataset contains records of 203 specimens of Diptera. All specimens have been morphologically identified to species level, and belong to 154 species in total. The species represented in this dataset correspond to about 10% of continental Portugal dipteran species diversity. All specimens were collected north of the Tagus river in Portugal. Sampling took place from 2014 to 2018, and specimens are deposited in the IBI collection at CIBIO, Research Center in Biodiversity and Genetic Resources. This dataset contributes to the knowledge on the DNA barcodes and distribution of 154 species of Diptera from Portugal and is the first of the planned IBI database public releases, which will make available genetic and distribution data for a series of taxa. All specimens have their DNA barcodes made publicly available in the Barcode of Life Data System (BOLD) online database and the distribution dataset can be freely accessed through the Global Biodiversity Information Facility (GBIF).

scholarly journals Metabarcoding free‐living marine nematodes using curated 18S and CO1 reference sequence databases for species‐level taxonomic assignments

2019 ◽  
Vol 9 (3) ◽  
pp. 1211-1226 ◽  
Author(s):  
Lara Macheriotou ◽  
Katja Guilini ◽  
Tania Nara Bezerra ◽  
Bjorn Tytgat ◽  
Dinh Tu Nguyen ◽  
...  
Keyword(s):  
Species Level ◽  
Reference Sequence ◽  
Marine Nematodes ◽  
Free Living ◽  
Sequence Databases ◽  
Taxonomic Assignments

scholarly journals Two practical approaches to monitoring the zooplanktonic community at Lago Grande do Curuai, Pará, Brazil

2015 ◽  
Vol 45 (3) ◽  
pp. 293-298 ◽  
Author(s):  
Leonardo Fernandes GOMES ◽  
Ludgero Cardoso Galli VIEIRA ◽  
Marie Paule BONNET
Keyword(s):  
Spatial Distribution ◽  
Monitoring Program ◽  
Zooplankton Community ◽  
Species Level ◽  
Genus Level ◽  
Time Costs ◽  
Financial Costs ◽  
Long Term Monitoring ◽  
Term Monitoring

The use of substitute groups in biomonitoring programs has been proposed to minimize the high financial costs and time for samples processing. The objectives of this study were to evaluate the correlation between (i) the spatial distribution among the major zooplankton groups (cladocerans, copepods, rotifers, and testaceans protozoa), (ii) the data of density and presence/absence of species, and (iii) the data of species, genera, and families from samples collected in the Lago Grande do Curuai, Pará, Brazil. A total of 55 sample of the zooplanktonic community was collected, with 28 samples obtained in March and 27 in September, 2013. The agreement between the different sets of data was assessed using Mantel and Procrustes tests. Our results indicated high correlations between genus level and species level and high correlations between presence/absence of species and abundance, regardless of the seasonal period. These results suggest that zooplankton community could be incorporated in a long-term monitoring program at relatively low financial and time costs.

scholarly journals Electrophoretic Resolution of Species Boundaries in Australian Microchiroptera. IV. The Molossidae (Chiroptera)

1988 ◽  
Vol 41 (3) ◽  
pp. 315 ◽  
Author(s):  
M Adams ◽  
TR Reardon ◽  
PR Baverstock ◽  
CHS Watts
Keyword(s):  
Species Level ◽  
Species Boundaries ◽  
Allozyme Electrophoresis ◽  
Single Specimen ◽  
Data Support ◽  
Electrophoretic Data ◽  
Current Species ◽  
Electrophoretic Resolution

Allozyme electrophoresis of 35 loci in 156 specimens of Australian bats belonging to the Molossidae was used to help elucidate the species-level taxonomy of the group in Australia. The electrophoretic data support the current species-level taxonomy of Tadarida australis and Chaerephon jobensis. However, for specimens currently allocated to the genus Mormopterus, the electrophoretic data fail to support any previous species-level account. On the electrophoretic data, a minimum of five species of the genus Mormopterus occur in Australia. A single specimen of a sixth species, whose generic affinities are undetermined, was also found.

scholarly journals First confirmed record of Trichobilharzia franki Müller & Kimmig, 1994, from Radix auricularia (Linnaeus, 1758) for Austria

2020 ◽  
Vol 119 (12) ◽  
pp. 4135-4141
Author(s):  
Susanne Reier ◽  
Elisabeth Haring ◽  
Florian Billinger ◽  
Hubert Blatterer ◽  
Michael Duda ◽  
...  
Keyword(s):  
Causative Agent ◽  
First Record ◽  
Species Level ◽  
Water Bodies ◽  
Intraspecific Diversity ◽  
Dna Barcodes ◽  
Cercarial Dermatitis ◽  
Avian Schistosomes ◽  
Veterinary Importance ◽  
Radix Auricularia

AbstractAvian schistosomes are of medical and veterinary importance as they are responsible for the annually occurring cercarial dermatitis outbreaks. For Austria, so far, only Trichobilharzia szidati Neuhaus 1952 was confirmed on species level as causative agent of cercarial dermatitis. Here we present the first record of Trichobilharzia franki Müller & Kimmig 1994 in Austria. The species was detected during a survey of digenean trematodes in Upper Austrian water bodies. Furthermore, we provide DNA barcodes of T. franki as well as measurements of several parasite individuals to indicate the intraspecific diversity. We also recommend the usage of an alternative primer pair, since the “standard COI primer pair” previously used for Schistosomatidae amplified an aberrant fragment in the sequence of T. franki. Overall, our study shows how limited our knowledge about occurrence and distribution of avian schistosomes in Austria is and how important it is to acquire such a knowledge to estimate ecological and epidemiological risks in the future.

scholarly journals Variant calling for cpn60 barcode sequence-based microbiome profiling

2019 ◽  
Author(s):  
Sarah J. Vancuren ◽  
Scott J. Dos Santos ◽  
Janet E. Hill ◽  
Keyword(s):  
De Novo ◽  
Sequence Data ◽  
Variant Calling ◽  
Taxonomic Composition ◽  
Species Level ◽  
Reference Sequence ◽  
Sequence Length ◽  
Sequence Variant ◽  
Operational Taxonomic Units ◽  
Microbiome Profiling

AbstractAmplification and sequencing of conserved genetic barcodes such as the cpn60 gene is a common approach to determining the taxonomic composition of microbiomes. Exact sequence variant calling has been proposed as an alternative to previously established methods for aggregation of sequence reads into operational taxonomic units (OTU). We investigated the utility of variant calling for cpn60 barcode sequences and determined the minimum sequence length required to provide species-level resolution. Sequence data from the 5’ region of the cpn60 barcode amplified from the human vaginal microbiome (n=45), and a mock community were used to compare variant calling to de novo assembly of reads, and mapping to a reference sequence database in terms of number of OTU formed, and overall community composition. Variant calling resulted in microbiome profiles that were consistent in apparent composition to those generated with the other methods but with significant logistical advantages. Variant calling is rapid, achieves high resolution of taxa, and does not require reference sequence data. Our results further demonstrate that 150 bp from the 5’ end of the cpn60 barcode sequence is sufficient to provide species-level resolution of microbiota.

scholarly journals Combining the 5.8S and ITS2 gene regions to improve classification of fungi

2019 ◽  
Author(s):  
Felix Heeger ◽  
Christian Wurzbacher ◽  
Elizabeth C. Bourne ◽  
Camila J. Mazzoni ◽  
Michael T. Monaghan
Keyword(s):  
High Variability ◽  
Species Level ◽  
Reference Sequence ◽  
Reference Database ◽  
List Type ◽  
Dna Metabarcoding ◽  
Automated Pipeline ◽  
Reference Sequences ◽  
Significant Underestimation

SummaryThe internal transcribed spacer (ITS) is used in DNA metabarcoding of fungi. One disadvantage of its high variability may be a failure to classify OTUs when no similar reference sequence exists. We tested whether the 5.8S region, often sequenced with ITS2 but discarded before analysis, could provide OUT classifications when ITS fails.We usedin silicoevaluation to compare classification success of 5.8S and ITS from the UNITE database when reference sequences of the same species, genus, or family were removed. We then developed an automated pipeline for a combined 5.8S - ITS2 analysis and applied it to mixed environmental samples containing many lineages that are underrepresented in databases.ITS was clearlysuperior for species-level classifications with a complete reference database, but 5.8S outperformed ITS at higher level classifications with an incomplete database. Our combined 5.8S-ITS2 pipeline classified 3x more fungal OTUs compared to ITS2 alone, particularly within Chytridiomycota (10x) and Rozellamycota (3x).Missing reference sequences led to the failure of ITS to classify many fungal OTUs at all, and to a significant underestimation of environmental fungal diversity. Using 5.8S to complement ITS classification will likely provide better estimates of diversity in lineages for which database coverage is poor.

On the distribution and ecology of Culiseta (Culicella) ochroptera (Peus) (Diptera: Culicidae) in Germany

Zootaxa ◽  
2019 ◽  
Vol 4576 (3) ◽  
pp. 544 ◽  
Author(s):  
CORNELIUS KUHLISCH ◽  
HELGE KAMPEN ◽  
DOREEN WERNER
Keyword(s):  
Mosquito Species ◽  
Monitoring Program ◽  
Ecological Data ◽  
The Past ◽  
Different Types ◽  
Human Use ◽  
Distribution And Ecology ◽  
Morphological Variants ◽  
Seasonal Population ◽  
Mosquito Monitoring

Culiseta ochroptera is a rare mosquito species in Germany, for which little ecological data are available. It is a species of peatlands where it occurs in a variety of habitats in different mire types. These landscape elements, strongly reduced and degraded by human use, are now often protected areas. Within the scope of a Germany-wide mosquito monitoring program, Cs. ochroptera was documented several times from 2011 to 2017, complementing historical data about its distribution and ecology. The records in northern and eastern Germany represent different types of peatlands and aquatic sites. Highest seasonal population densities were recorded in July and August, caused by the second of at least two annual generations. The morphological variants of Cs. ochroptera as described in the past were assigned to three forms (light, normal, dark), and the previously noted but neglected presence of postspiracular scales is recognized as a useful character supporting species identification. 

Revision of the unusual Carboniferous ophiuroid Cholaster (Echinodermata) and remarks on skeletal differentiation within the Asterozoa

2019 ◽  
Vol 93 (04) ◽  
pp. 753-763
Author(s):  
Daniel B. Blake ◽  
Merlynd K. Nestell
Keyword(s):  
Small Area ◽  
Skeletal Remains ◽  
Species Level ◽  
Single Specimen ◽  
Cedar Creek ◽  
Early Ordovician ◽  
Dorsal Aspect

AbstractNewly discovered, relatively well-preserved specimens ofCholaster whitein. sp. (Ophiuroidea, Echinodermata) are described from a small area of extensive outcrop of the Bangor Limestone (Mississippian, Chesterian) exposed on the edge of Cedar Creek Reservoir in northern Alabama, USA. The only other known species of the genus,C.peculiarisWorthen and Miller, is based on a single specimen exposed in dorsal aspect and collected from strata of similar age from southwestern Illinois. Incomplete preservation of the singleC.peculiarisspecimen limits comparisons, but differences between the two occurrences support separation at the species level.Skeletal remains of both asteroids and ophiuroids are first recognized from Early Ordovician sediments, and representatives of the two classes have retained plesiomorphies or converged morphologically since that time, thereby suggesting important evolutionary potentials and limitations.Cholasteris asteroid-like and unusual among ophiuroids in that the arms are comparatively broad and strap-like, and lateral ossicles are similar to asteroid adambulacrals and marginals, whereas the “vertebrae” (i.e., fused axial pair) and oral frame configurations ofC. whitein. sp. are typical of the Ophiuroidea. The oral frame ofC. peculiarisis unknown.A poorly preserved specimen of the asteroidDelicaster? also was recovered from nearby strata associated with theC. whitein. sp.UUID:http://zoobank.org/e0eea445-58e5-4096-80c1-a65964832ef6

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