Comparison of biomass production and total lipid content of freshwater green microalgae cultivated under various culture conditions

Wastewater treatment High Rate Algal Ponds with CO2 addition could provide cost-effective and efficient tertiary-level wastewater treatment with the co-benefit of algal biomass production for biofuel use. Wastewater grown algal biomass can have a lipid content of 10–30% of dry weight, which could be used to make biodiesel. This research investigated algal biomass and total lipid production by two pilot-scale wastewater treatment HRAPs (4-day HRT) with and without CO2 addition under New Zealand mid summer (Nov–Jan) conditions. The influence of CO2 addition on wastewater treatment performance was also determined. CO2 was added to one of the HRAPs (the HRAPE) by maintaining the maximum pH of the pond below 8. Measurements of HRAP influent and effluent water qualities, total lipid content and algal biomass production were made twice a week over the experimental period. Both HRAPs achieved high levels of organic compound and nutrient removal, with >85% SBOD5, >92 NH4+-N and >70% DRP removal. Algal/bacterial biomass production in the HRAPE (15.2 g/m2/d) was improved by CO2 addition by ∼30% compared with that of the control HRAPW (10.6 g/m2/d). Total lipid content of the biomass grown on both HRAPs was slightly reduced (from 25% to 20%) with CO2 addition and the maximum total lipid content of ∼40% was observed in the HRAPW when low NH4+-N concentration (<0.5 mg/L) and high maximum pH (>10.0) occurred. Total lipid content of the biomass increased by ∼15% under nitrogen limiting conditions, however, overall algal/bacterial biomass production was reduced by half during the period of nitrogen limitation. More research is required to maintain algal production under near nitrogenlimiting conditions.

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Impact of Seasonal Changes on Composition of Total Lipid Content in Muscle, Liver and Testes of Freshwater Male Fish Mystus Cavasius (Ham) From Bhadra Reservoir, Shivamogga, Karnataka, India

International Journal of Scientific Research ◽

10.15373/22778179/feb2014/181 ◽

2012 ◽

Vol 3 (2) ◽

pp. 540-541

Author(s):

Ashashree H M Ashashree H M ◽

◽

B.Venkateshwarlu B B.Venkateshwarlu B ◽

Sayeswara HA Sayeswara HA

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Seasonal Changes ◽

Total Lipid Content ◽

Male Fish

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Aspergillus flavusgrowth and aflatoxin production as influenced by total lipid content during growth and development of cottonseed

Journal of Crop Improvement ◽

10.1080/15427528.2016.1263811 ◽

2017 ◽

Vol 31 (1) ◽

pp. 91-99 ◽

Cited By ~ 2

Author(s):

Kanniah Rajasekaran ◽

Greg Ford ◽

Kandan Sethumadhavan ◽

Carol Carter-Wientjes ◽

John Bland ◽

...

Keyword(s):

Total Lipid ◽

Lipid Content ◽

Growth And Development ◽

Total Lipid Content ◽

Aflatoxin Production

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Adipophilin and Perilipin 3 Correlate with Total Lipid Content in Human Breast Milk

10.20944/preprints201810.0008.v1 ◽

2018 ◽

Author(s):

Tereza Pavlova ◽

Zdenek Spacil ◽

Veronika Vidova ◽

Filip Zlamal ◽

Eliska Cechova ◽

...

Keyword(s):

Mass Spectrometry ◽

Breast Milk ◽

Total Lipid ◽

Lipid Content ◽

Gravimetric Method ◽

Total Lipid Content ◽

Milk Lipid ◽

Human Breast Milk ◽

Human Breast

Objective: Lipids are secreted into milk as bilayer-coated structures: milk lipid globules (MLGs). Adipophilin (ADRP) and perilipin 3 (TIP47) are associated with MLGs in human breast milk; however, the role of these proteins in milk lipid secretion is not fully understood. The aim of the study was to investigate levels of ADRP, TIP47 and total lipid content in human breast milk, their mutual correlations and dynamics during lactation. Research Methods & Procedures: Milk samples from 22 healthy lactating women (Caucasian, Central European) were collected at five time points during lactation (1–3, 12–14, 29–30, 88–90 and 178–180 days postpartum). Mass spectrometry-based method was used for quantification of ADRP and TIP47 in the samples. The gravimetric method was used to determine milk total lipid content. Results: We observed distinctive trends in ADRP, TIP47 levels and lipid content in human breast milk during the first 6 months of lactation. We also found a significant association between lipid content and ADRP, lipid content and TIP47, and ADRP and TIP47 concentrations in breast milk at all sampling points. Moreover, we derived an equation for estimating the mean lipid content of milk based on ADRP concentration in human breast milk. Conclusions: A mass spectrometry-based method was developed for quantifying ADRP and TIP47 in human breast milk. Strong mutual correlations were found between ADRP, TIP47 and total lipid content in human breast milk.

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