Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid

2019 ◽  
Vol 43 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Miloš Radosavljević ◽  
Steva Lević ◽  
Miona Belović ◽  
Jelena Pejin ◽  
Aleksandra Djukić-Vuković ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Polyvinyl Alcohol ◽  
Calcium Alginate ◽  
Lactobacillus Rhamnosus ◽  
Alginate Matrix

Comparative study on polyvinyl alcohol and alginate for cell immobilization in biosorption

2000 ◽  
Vol 42 (5-6) ◽  
pp. 85-90 ◽  
Author(s):  
Y.P. Ting ◽  
G. Sun
Keyword(s):  
Polyvinyl Alcohol ◽  
Calcium Alginate ◽  
Dilute Hydrochloric Acid ◽  
Cell Immobilization ◽  
Chemical Properties ◽  
Yeast Cells ◽  
Lower Mass ◽  
Transfer Resistance ◽  
Alginate Matrix ◽  
And Chemical Properties

In this work, we compared the performance of a new method of cell immobilization in a novel matrix for biosorption. Yeast cells were entrapped in a polyvinyl alcohol (PVA) matrix, based on an adapted iterative freeze-thaw-freeze process. Spherical and uniform beads were produced, and SEM micrographs confirmed that the cells were uniformly dispersed within the PVA matrix. Further experiments revealed that the use of PVA as the immobilization matrix conferred better mechanical and chemical properties than the commonly used calcium alginate matrix. Experiments also showed that the PVA matrix gave rise to a lower mass transfer resistance than the alginate matrix. Finally, it was established that PVA-yeast biosorbent beads could be regenerated using dilute hydrochloric acid (10mM) and reused for at least five biosorption cycles with virtually no decrease in its bisorption capacity. Different metal/biosorbent in PVA-immobilized systems are currently being investigated.

Encapsulation of Lactobacillus rhamnosus in Polyvinyl Alcohol for the production of L-(+)-Lactic Acid

2021 ◽  
Vol 100 ◽  
pp. 149-160
Author(s):  
Miloš Radosavljević ◽  
Steva Lević ◽  
Miona Belović ◽  
Jelena Pejin ◽  
Aleksandra Djukić-Vuković ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Polyvinyl Alcohol ◽  
Lactobacillus Rhamnosus

Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

2021 ◽  
Author(s):  
Rosetânia Correia Neves da Conceição ◽  
Rayssa Dias Batista ◽  
Fernanda Munhoz dos Anjos Leal Zimmer ◽  
Ianna Kelly Martins Trindade ◽  
Alex Fernando de Almeida ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Lactobacillus Paracasei ◽  
Gelatin Coating ◽  
Alginate Matrix

scholarly journals Biotransformation of Polyphenols in Apple Pomace Fermented by β-Glucosidase-Producing Lactobacillus rhamnosus L08

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1343
Author(s):  
Lihua Liu ◽  
Chenyi Zhang ◽  
Huimin Zhang ◽  
Guoqiang Qu ◽  
Chun Li ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Food Industry ◽  
Antioxidant Activities ◽  
Lactobacillus Rhamnosus ◽  
Economic Benefits ◽  
Apple Pomace ◽  
High Cell ◽  
Bioactive Components ◽  
Glucosidase Activity ◽  
Phenolic Profiles

Apple pomace, the main by-product in apple processing, is a cheap source of bioactive compounds that could be used in the food industry. However, the value of this by-product is still far from being fully realized. In this study, 11 strains of Lactobacillus strains were assayed for β-glucosidase activity, and only Lactobacillus rhamnosus L08 (L. rhamnosus L08) showed high cell-membrane associated β-glucosidase activity. We then evaluated the effects of fermentation of apple pomace using the selected strain, focusing on the biotransformation of polyphenols and antioxidant capacity. We found that L. rhamnosus L08 fermentation significantly reduced the contents of quercitrin and phlorizin in apple pomace, while increasing the contents of quercetin and phloretin. The contents of gallic acid, epicatechin acid, caffeic acid, and ferulic acid were also increased in apple pomace after fermentation. In addition, the antioxidant activities of apple pomace were enhanced during fermentation, based on the bioconversion of phenolic profiles. Our results demonstrate that lactic acid bacteria fermentation is a promising approach to enhance the bioactivity of phenolic compounds in apple pomace. Moreover, this study demonstrates that, as a valuable processing by-product with bioactive components, apple pomace can be used in the food industry to provide economic benefits.

Immobilization of Rhizopus oryzae in a modified polyvinyl alcohol gel for L(+)-lactic acid production

2012 ◽  
Vol 63 (3) ◽  
pp. 957-964 ◽  
Author(s):  
Peng Wang ◽  
Zhen Chen ◽  
Juan Li ◽  
Li Wang ◽  
Guohong Gong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Polyvinyl Alcohol ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Lactic Acid Production

scholarly journals pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

2005 ◽  
Vol 71 (10) ◽  
pp. 6008-6013 ◽  
Author(s):  
Domitille Fayol-Messaoudi ◽  
Cédric N. Berger ◽  
Marie-Hélène Coconnier-Polter ◽  
Vanessa Liévin-Le Moal ◽  
Alain L. Servin
Keyword(s):  
Lactic Acid ◽  
Growth Phase ◽  
Salmonella Enterica ◽  
Lactobacillus Rhamnosus ◽  
Stationary Growth Phase ◽  
Exponential Growth Phase ◽  
Killing Activity ◽  
Serovar Typhimurium ◽  
Probiotic Strains ◽  
Probiotic Lactobacilli

ABSTRACT The mechanism(s) underlying the antibacterial activity of probiotic Lactobacillus strains appears to be multifactorial and includes lowering of the pH and the production of lactic acid and of antibacterial compounds, including bacteriocins and nonbacteriocin, non-lactic acid molecules. Addition of Dulbecco's modified Eagle's minimum essential medium to the incubating medium delays the killing activity of lactic acid. We found that the probiotic strains Lactobacillus johnsonii La1, Lactobacillus rhamnosus GG, Lactobacillus casei Shirota YIT9029, L. casei DN-114 001, and L. rhamnosus GR1 induced a dramatic decrease in the viability of Salmonella enterica serovar Typhimurium SL1344 mainly attributable to non-lactic acid molecule(s) present in the cell-free culture supernatant (CFCS). These molecules were more active against serovar Typhimurium SL1344 in the exponential growth phase than in the stationary growth phase. We also showed that the production of the non-lactic acid substance(s) responsible for the killing activity was dependent on growth temperature and that both unstable and stable substances with killing activity were present in the CFCSs. We found that the complete inhibition of serovar Typhimurium SL1344 growth results from a pH-lowering effect.

scholarly journals Swelling behavior of polyvinyl alcohol and lactic acid hydrogel films

2016 ◽  
Vol 11 (1) ◽  
pp. 102-103 ◽  
Author(s):  
Jirapornchai Suksaeree ◽  
Chitradee Luprasong ◽  
Chaowalit Monton
Keyword(s):  
Lactic Acid ◽  
Polyvinyl Alcohol ◽  
Swelling Behavior ◽  
Hydrogel Films

scholarly journals The effect of bacterial inoculant supplementation on the fermentation process of crushed maize ears in laboratory silos

2005 ◽  
Vol 53 (4) ◽  
pp. 33-42
Author(s):  
Petr Doležal ◽  
Dušan Kořínek ◽  
Jan Doležal ◽  
Václav Pyrochta
Keyword(s):  
Lactic Acid ◽  
Ph Value ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Brevis ◽  
Water Soluble ◽  
Bacterial Strains ◽  
Total Acid ◽  
Ethanol Content ◽  
Fermentation Quality ◽  
Biological Additive

In the experiment was the effect of biological additive on the fermentation quality of crushed maize ears of two hybrids by comparing with the untreated control. The bacterial inoculant „A“ contained selected bacterial strains of Lactobacillus rhamnosus (NCIMB 30121) and Enterococcus faecium (NCIMB 30122). As effective substances of bacterial water–soluble inoculant „B“ were selected bacterial strains of Lactobacillus rhamnosus (NCIMB 30121), Lactobacillus plantarum (DSM 12836), Lactobacillus brevis (DSM 12835), Lactobacillus buchneri (DSM 12856), Pediococcus acidialactici (P. pentosaceus) (DSM 12834). The addition of inoculant „A“ in our experiment conditions increased statistically significantly (P<0.01) the pH value (4.09±0.01), resp. 4.02±0.02 in second trial with Pedro hybrid. The bacterial inoculant „B“ increased significantly (P<0.01) the contents of lactic acid (50.95±0.1.87 g/kg DM), acetic acid (18.61±0.34 g/kg DM), sum of acids (69.55±1.62 g/kg DM) and decreased (P<0.01) in the first trial the ethanol content (5.41±0.45 g/kg DM). The highest DM content (P<0.01) was in all experimental inoculated silages with additive „A“ (54.26±0.86%, and 53.56±0.54%, resp.). The bacterial inoculant „A“ increased significantly (P<0.01) in comparison with control silage in the second trial the content of lactic acid (34.66Ī2.81 g/kg DM), sum of acids (44.68±3.54 g/kg DM), the total acids content (32.87±2.88 g/kg DM), and ethanol content (17.33±0.79 g/kg DM). The inoculation positive effect was demonstrated in reduction of ethanol amount and of total acid production. The pH value of inoculated silages was not significantly lower than that in the control silage.

scholarly journals Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Preliminary hydrolysis and fermentation studies

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
WHINY HARDIYATI ERLIANA ◽  
Tri Widjaja ◽  
ALI ALTWAY ◽  
LILY PUDJIASTUTI
Keyword(s):  
Lactic Acid ◽  
Lignin Content ◽  
Agricultural Waste ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Brevis ◽  
Reducing Sugar ◽  
Cellulose Content ◽  
Acid Pretreatment ◽  
Acid Fermentation

Abstract. Erliana WH, Widjaja T, Altway A, Pudjiastuti L. 2020. Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Hydrolysis and fermentation studies. Biodiversitas 21: 2281-2288. The increasing problems of global energy and the environment are the main reasons for developing products with new techniques through green methods. Sugar palm trunk waste (SPTW) has potential as agricultural waste because of its abundant availability, but it is not used optimally. This study was aimed to determine the effect of various microorganisms on increasing lactic acid production by controlling pH and temperature conditions in the fermentation process. SPTW contains 43.88% cellulose, 7.24% hemicellulose, and 33.24% lignin. The lignin content in SPTW can inhibit reducing sugar formation; the pretreatment process should remove this content. In the study, the pretreatment process was conducted using acid-organosolv. In the acid pretreatment, 0.2 M H2SO4 was added at 120oC for 40 minutes; organosolv pretreatment using 30% ethanol (v/v) at 107oC for 33 minutes was able to increase cellulose content by 56.33% and decrease lignin content by 27.09%. The pretreatment was followed by an enzymatic hydrolysis process with a combination of commercial cellulase enzymes from Aspergillus niger (AN) and Trichoderma reesei (TR), with variations of 0:1, 1:0, 1:1, 1:2 and 2:1. The best reducing sugar concentration was obtained with an AN: TR ratio of 1:2 to form reducing sugar from cellulose. Subsequently, lactic acid fermentation was carried out using lactic acid bacteria at 37oC and pH 6 incubated for 48 hours. The highest lactic acid concentration (33.292 g/L) was obtained using a mixed culture of Lactobacillus rhamnosus and Lactobacillus brevis to convert reducing sugar become lactic acid.

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