Evaluation of the in vitro cell-material interactions and in vivo osteo-integration of a spinal acrylic bone cement

AbstractCell fate is largely determined by interactions that occur at the interface between cells and their surrounding microenvironment. For this reason, especially in the field of cell- and tissue-engineering, there is a growing interest in developing characterization techniques that allow a deep evaluation of cell-material interaction at the nanoscale, particularly focusing on cell adhesion processes. While for 2D culturing systems a consolidated series of tools already satisfy this need, in 3D environments, more closely recapitulating complex in vivo structures, there is still a lack of procedure furthering the comprehension of cell-material interactions. Here, we report for the first time the use of a SEM/FIB system for the characterization of cellular adhesion in 3D scaffolds fabricated by means of different techniques. Our results clearly show the capability of the developed approach to finely resolve both scaffold-cells interface and nanometer scale features of cell bodies involved in the upregulation of cellular behavior. These results are relevant for studying cellular guidance strategies and for the consequent design of more efficient cell-instructive platforms for tissue-engineering applications as well as for in vitro 3D models.

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Mechanical Performance and In Vivo Tests of an Acrylic Bone Cement Filled with Bioactive Sepia Officinalis Cuttlebone

Journal of Biomaterials Science Polymer Edition ◽

10.1163/156856209x410265 ◽

2010 ◽

Vol 21 (1) ◽

pp. 113-125 ◽

Cited By ~ 14

Author(s):

S. García-Enriquez ◽

H. E. R. Guadarrama ◽

I. Reyes-González ◽

E. Mendizábal ◽

C. F. Jasso-Gastinel ◽

...

Keyword(s):

Bone Cement ◽

Mechanical Performance ◽

Sepia Officinalis ◽

Acrylic Bone Cement ◽

In Vivo Tests

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Development of Novel Bioactive PMMA-Based Bone Cement and its In Vitro and In Vivo Evaluation

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.801 ◽

2006 ◽

Vol 309-311 ◽

pp. 801-804 ◽

Cited By ~ 3

Author(s):

S.B. Cho ◽

Akari Takeuchi ◽

Ill Yong Kim ◽

Sang Bae Kim ◽

Chikara Ohtsuki ◽

...

Keyword(s):

Mechanical Property ◽

Compressive Strength ◽

Bone Cement ◽

The Novel ◽

In Vivo Evaluation ◽

Natural Bone ◽

Pmma Bone Cement ◽

Rabbit Tibia

In order to overcome the disadvantage of commercialized PMMA bone cement, we have developed novel PMMA-based bone cement(7P3S) reinforced by 30 wt.% of bioactive CaO-SiO2 gel powders to induce the bioactivity as well as to increase mechanical property for the PMMA bone cement. The novel 7P3S bone cement hardened after mixing for about 7 minutes. For in vitro evaluation, apatite forming ability of it was investigated using SBF. When the novel 7P3S bone cement was soaked into SBF, it formed apatite on its surfaces within 1 week Furthermore; there is no decrease in its compressive strength within 9 weeks soaking in SBF. It is though that hardly decrease in compressive strength of 7P3S bone cement in SBF is due to the relative small amount of gel powder or its spherical shape and monosize. In vivo evaluation of the novel 7P3S bone cement was carried out using rabbit. After implantion into rabbit tibia for several periods, the interface between novel bone cement and natural bone was evaluated by CT images. According to the results, the novel bone cement directly contact to the natural bone without fibrous tissue after implantation for 4 weeks. This results indicates that the newly developed 7P3S bone cement can bond to the living bone and also be effectively used as bioactive bone cement without decrease in mechanical property.

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The chitosan/tri-calcium phosphate bio-composite bone cement promotes better osteo-integration: an in vitro and in vivo study

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-019-1201-2 ◽

2019 ◽

Vol 14 (1) ◽

Cited By ~ 4

Author(s):

Chih-Hsiang Fang ◽

Yi-Wen Lin ◽

Jui-Sheng Sun ◽

Feng-Huei Lin

Keyword(s):

Calcium Phosphate ◽

Bone Cement ◽

In Vivo Study ◽

Composite Bone

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Osseointegration of Antimicrobial Acrylic Bone Cements Modified with Graphene Oxide and Chitosan

Applied Sciences ◽

10.3390/app10186528 ◽

2020 ◽

Vol 10 (18) ◽

pp. 6528 ◽

Cited By ~ 2

Author(s):

Mayra Eliana Valencia Zapata ◽

José Herminsul Mina Hernandez ◽

Carlos David Grande Tovar ◽

Carlos Humberto Valencia Llano ◽

Blanca Vázquez-Lasa ◽

...

Keyword(s):

Graphene Oxide ◽

Bone Cement ◽

Orthopedic Surgery ◽

Bending Strength ◽

Orthopedic Implants ◽

Parietal Bone ◽

Maximum Temperature ◽

Cement Interface

Acrylic bone cement (ABC) is one of the most used materials in orthopedic surgery, mainly for the fixation of orthopedic implants to the bone. However, ABCs usually present lack of biological activity and osseointegration capacity that leads to loosening of the prosthesis. This work reports the effect of introducing graphene oxide (GO) and chitosan (CS), separately or together, in the ABC formulation on setting performance, mechanical behavior, and biological properties. Introduction of both CS and GO to the ABC decreased the maximum temperature by 21% and increased the antibacterial activity against Escherichia coli by 87%, while introduction of only CS decreased bending strength by 32%. The results of cell viability and cell adhesion tests showed in vitro biocompatibility. The in vivo response was investigated using both subdermal and bone parietal implantations in Wistar rats. Modified ABCs showed absence of immune response, as confirmed by a normal inflammatory response in Wistar rat subdermal implantation. The results of the parietal bone implantation showed that the addition of CS and GO together allowed a near total healing bone–cement interface, as observed in the micrographic analysis. The overall results support the great potential of the modified ABCs for application in orthopedic surgery mainly in those cases where osseointegration is required.

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