Electrochemical detection of in situ DNA damage induced by enzyme-catalyzed Fenton reaction. Part I: in phosphate buffer solution

2012 ◽  
Vol 178 (1-2) ◽  
pp. 37-43 ◽  
Author(s):  
Yang Chen ◽  
Huayu Xiong ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Dna Damage ◽  
Electrochemical Detection ◽  
Phosphate Buffer ◽  
Fenton Reaction ◽  
Phosphate Buffer Solution ◽  
Buffer Solution

Ibuprofen removal from aqueous solution by in situ electrochemically generated ferrate(VI): proof-of-principle

2015 ◽  
Vol 73 (2) ◽  
pp. 389-395 ◽  
Author(s):  
Ljiljana Nikolić-Bujanović ◽  
Milan Čekerevac ◽  
Milena Tomić ◽  
Mladen Zdravković
Keyword(s):  
Aqueous Solution ◽  
Organic Carbon ◽  
Aqueous Solutions ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Oxygen Demand ◽  
Buffer Solution ◽  
Effective Removal ◽  
Proof Of Principle

The possibility of removing pharmaceuticals from aqueous solutions was examined using ibuprofen (Ibu) oxidation as an example, using in situ electrochemically synthesized ferrate(VI), a strong oxidant and coagulant, with forming of non-harmful byproducts. A solution of ibuprofen of 206 mg/L in 0.1 M phosphate buffer solution was treated with different amounts of fresh, electrochemically synthesized ferrate(VI). The changes of ibuprofen concentration in samples were determined using a UV-Vis spectrophotometer. The extent of mineralization was estimated using the changes in chemical oxygen demand (COD) values and total organic carbon (TOC) values of test samples. The largest reduction of the concentration of Ibu (41.75%) was obtained by adding 69.2 mg/L ferrate(VI) as Fe (Ibu: Fe = 1: 0.34). An effective removal of ibuprofen from aqueous solutions was recorded up to 68% and it can be done by using ferrate(VI) in the ratio Ibu: Fe = 1:3 as Fe. The possibility of ibuprofen removal by ferrate(VI) was confirmed by COD and TOC results, which demonstrated reduction up to 65% and 63.6%, respectively.

In-Situ Observation of Nanowire Growth from Luminescent CdTe Nanocrystals in a Phosphate Buffer Solution

ChemPhysChem ◽  
2004 ◽  
Vol 5 (10) ◽  
pp. 1600-1602 ◽  
Author(s):  
Yuri Volkov ◽  
Siobhan Mitchell ◽  
Nikolai Gaponik ◽  
Yurii P. Rakovich ◽  
John F. Donegan ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Nanowire Growth ◽  
In Situ Observation ◽  
Buffer Solution ◽  
Cdte Nanocrystals

scholarly journals Crosslinking Efficacy and Cytotoxicity of Genipin and Its Activated Form Prepared by Warming It in a Phosphate Buffer: A Comparative Study

Materials ◽  
2021 ◽  
Vol 14 (21) ◽  
pp. 6600
Author(s):  
Takeya Kawamura ◽  
Shunji Yunoki ◽  
Yoshimi Ohyabu ◽  
Toshio Uraoka ◽  
Kazuaki Muramatsu
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Mechanical Tests ◽  
Buffer Solution ◽  
Sodium Phosphate Buffer ◽  
In Situ Forming ◽  
Custom Made ◽  
Acute Cytotoxicity ◽  
Sodium Phosphate Buffer Solution

The aim of the present study was to compare the acute and cumulative cytotoxicity of intact (n-GE) and warmed genipin (w-GE), while investigating the differences in crosslinking capabilities of these two genipins by rheological and mechanical tests. The n-GE solution was prepared by dissolving genipin powder in a sodium phosphate buffer solution. The w-GE solution was prepared by warming the n-GE solution at 37 °C for 24 h. The mechanical tests for chitosan (CH)/genipin gels showed the crosslinking rate of w-GE was much greater than that of n-GE up until 6 h after preparation, whereas the degree of crosslinking of CH/n-GE gels became higher at 12 h. The ISO 10993-5 standard method, which is established specifically for evaluating cumulative cytotoxicity, determined equivalent IC50 for w-GE (0.173 mM) and n-GE (0.166 mM). On the other hand, custom-made cytotoxicity tests using a WST-8 assay after 1 h of cultivation showed that the acute cytotoxicity of w-GE was significantly higher than that of n-GE at concentrations between 0.1–5 mM. The acute cytotoxicity of w-GE should be taken into consideration in its practical uses, despite the fact that the much faster crosslinking of w-GE is useful as an effective cross linker for in-situ forming gels.

scholarly journals Cover Picture: In-Situ Observation of Nanowire Growth from Luminescent CdTe Nanocrystals in a Phosphate Buffer Solution (ChemPhysChem 10/2004)

ChemPhysChem ◽  
2004 ◽  
Vol 5 (10) ◽  
pp. 1457-1457
Author(s):  
Yuri Volkov ◽  
Siobhan Mitchell ◽  
Nikolai Gaponik ◽  
Yurii P. Rakovich ◽  
John F. Donegan ◽  
...  
Keyword(s):  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Nanowire Growth ◽  
In Situ Observation ◽  
Buffer Solution ◽  
Cdte Nanocrystals

Bioceramic Hydroxyapatite Granules for Purification of Biotechnological Products

2011 ◽  
Vol 284-286 ◽  
pp. 1764-1769 ◽  
Author(s):  
Vitalijs Lakevics ◽  
Janis Locs ◽  
Dagnija Loca ◽  
Valentina Stepanova ◽  
Liga Berzina-Cimdina ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Phosphate Buffer ◽  
Bovine Serum ◽  
Phosphate Buffer Solution ◽  
Sorption Process ◽  
Buffer Solution ◽  
Ph Values ◽  
Bovine Serum Albumin Adsorption ◽  
Albumin Adsorption

Sorption experiments of bovine serum albumin (BSA) on hydroxyapatite (HAp) ceramic granules, prepared at three temperatures 900°C, 1000°C and 1150°C were performed at room temperature 18,6 °C and phosphate buffer, pH 5,83; 6.38 and 7,39. Thermal treatment contributed to the decrease of bovine serum albumin immobilization indicating that sorption process depended on HAp ceramics specific surface area and pH values of phosphate buffer solution. However, it was confirmed that granule size was also an important parameter for bovine serum albumin adsorption. As a result of these experiments, the most appropriate adsorption conditions and phosphate buffer pH values influence on to BSA sorption were analyzed.

Reduction of Escherichia coli O157:H7 Counts on Whole Fresh Apples by Treatment with Sanitizers

2000 ◽  
Vol 63 (6) ◽  
pp. 703-708 ◽  
Author(s):  
MARCY A. WISNIEWSKY ◽  
BONITA A. GLATZ ◽  
MARK L. GLEASON ◽  
CHERYLL A. REITMEIER
Keyword(s):  
Escherichia Coli ◽  
Chlorine Dioxide ◽  
Phosphate Buffer ◽  
Phosphate Buffer Solution ◽  
Escherichia Coli O157 ◽  
Peroxyacetic Acid ◽  
Water Control ◽  
Buffer Solution ◽  
E Coli ◽  
Log Reduction

The objectives of this study were to determine if washing of whole apples with solutions of three different sanitizers (peroxyacetic acid, chlorine dioxide, or a chlorine-phosphate buffer solution) could reduce a contaminating nonpathogenic Escherichia coli O157:H7 population by 5 logs and at what sanitizer concentration and wash time such a reduction could be achieved. Sanitizers were tested at 1, 2, 4, 8, and 16 times the manufacturer's recommended concentration at wash times of 5, 10, and 15 min. Whole, sound Braeburn apples were inoculated with approximately 1 × 108 or 7 × 106 CFU per apple, stored for 24 h, then washed with sterile water (control) or with sanitizers for the prescribed time. Recovered bacteria were enumerated on trypticase soy agar. Washing with water alone reduced the recoverable population by almost 2 logs from the starting population; this can be attributed to physical removal of organisms from the apple surface. No sanitizer, when used at the recommended concentration, reduced the recovered E. coli population by 5 logs under the test conditions. The most effective sanitizer, peroxyacetic acid, achieved a 5-log reduction when used at 2.1 to 14 times its recommended concentration, depending on the length of the wash time. The chlorine-phosphate buffer solution reduced the population by 5 logs when used at 3 to 15 times its recommended concentration, depending on wash time. At no concentration or wash time tested did chlorine dioxide achieve the 5-log reduction.

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