Microwave-assisted synthesis of highly luminescent N- and S-co-doped carbon dots as a ratiometric fluorescent probe for levofloxacin

2018 ◽  
Vol 185 (2) ◽  
Author(s):  
Huiyu Li ◽  
Yuan Xu ◽  
Jie Ding ◽  
Li Zhao ◽  
Tianyu Zhou ◽  
...  
Chemosensors ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 61
Author(s):  
Fajar Amelia Rachmawati Putri ◽  
Mudasir Mudasir ◽  
Kinichi Morita ◽  
Suherman Suherman

Fluorescent amikacin modified nitrogen, sulfur co-doped carbon dots (amikacin modified N,S-CDs) were synthesized by a facile and low-cost one-step microwave-assisted specifically for selective detection of Gram-negative bacteria Escherichia coli (E. coli). Amikacin is a semi-synthetic amino glycoside antibiotic and it was employed in this study to increase the fluorescence response of N,S-CDs by providing binding ligand towards E. coli. The effect of thiourea content as the source of nitrogen and sulfur dopants was investigated prior to the preparation of amikacin modified N,S-CDs. The formation of amikacin modified N,S-CDs were characterized by using Fourier transform infrared (FTIR), X-ray diffraction (XRD), Transmission electron microscope (TEM), UV-Vis spectrophotometer, and spectrofluorometer. Amikacin modified N,S-CDs was identified to be successfully synthesized from the wavenumber shift of the C=O stretching mode. Amikacin modified N,S-CDs were amorphous with an average size of 7 nm. Fluorescence spectra showed that the highest intensity was obtained at thiourea content of 50% and amikacin mass of 25 mg. By comparing fluorescence responses of all the investigated amikacin modified N,S-CDs, the limit of detection (LOD) was attained by 25 mg amikacin modified N,S-CDs at 1.526 cfu mL−1.


Nanoscale ◽  
2015 ◽  
Vol 7 (41) ◽  
pp. 17278-17282 ◽  
Author(s):  
Xiangcheng Sun ◽  
Christian Brückner ◽  
Yu Lei

Microwave-assisted synthesis of nitrogen and phosphorus co-doped carbon dots with high quantum yield and dual (blue and green) fluorescence emission.


2018 ◽  
Vol 6 (36) ◽  
pp. 9636-9641 ◽  
Author(s):  
Zhi Shen ◽  
Cheng Zhang ◽  
Xinling Yu ◽  
Jian Li ◽  
Zhenyang Wang ◽  
...  

Fluorescent carbon dots were synthesized by microwave irradiation of cyclen and citric acid to construct a ratiometric probe for tetracycline detection.


2018 ◽  
Vol 15 (1) ◽  
pp. 47-55
Author(s):  
Xuebing Li ◽  
Haifen Yang ◽  
Ning Wang ◽  
Tijian Sun ◽  
Wei Bian ◽  
...  

Background: Morin has many pharmacological functions including antioxidant, anticancer, anti-inflammatory, and antibacterial effects. It is commonly used in the treatment of antiviral infection, gastropathy, coronary heart disease and hepatitis B in clinic. However, researches have shown that morin is likely to show prooxidative effects on the cells when the amount of treatment is at high dose, leading to the decrease of intracellular ATP levels and the increase of necrosis process. Therefore, it is necessary to determine the concentration of morin in biologic samples. Method: Novel water-soluble and green nitrogen and sulfur co-doped carbon dots (NSCDs) were prepared by a microwave heating process with citric acid and L-cysteine. The fluorescence spectra were collected at an excitation wavelength of 350 nm when solutions of NSCDs were mixed with various concentrations of morin. Results: The as-prepared NSCDs were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The fluorescence intensity of NSCDs decreased significantly with the increase of morin concentration. The fluorescence intensity of NSCDs displayed a linear response to morin in the concentration 0.10-30 μM with a low detection limit of 56 nM. The proposed fluorescent probe was applied to analysis of morin in human body fluids with recoveries of 98.0-102%. Conclusion: NSCDs were prepared by a microwave heating process. The present analytical method is sensitive to morin. The quenching process between NSCDs and morin is attributed to the static quenching. In addition, the cellular toxicity on HeLa cells indicated that the as-prepared NSCDs fluorescent probe does not show obvious cytotoxicity in cell imaging. Our proposed method possibly opens up a rapid and nontoxic way for preparing heteroatom doped carbon dots with a broad application prospect.


2021 ◽  
Author(s):  
Shreya Bhatt ◽  
Gaurav Vyas ◽  
Parimal Paul

Preparation of water dispersible carbon dots from inexpensive natural carbon precursors and its application for purposes such as sensing, bio-imaging, patterning agent etc. is showing growing interest in recent years....


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