Suspension array for multiplex immunoassay of five common endocrine disrupter chemicals

To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively) were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100%), diethofencarb (92.08%), and iprodione (86.59%) were detected. BosR isolates accounted for 11.67% of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55%) possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99%) mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50%), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR) and only one F200Y mutant was moderately resistant (MR) to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field.

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Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays

Malaria Journal ◽

10.1186/1475-2875-10-192 ◽

2011 ◽

Vol 10 (1) ◽

pp. 192 ◽

Cited By ~ 24

Author(s):

Edmilson Rui ◽

Carmen Fernandez-Becerra ◽

Satoru Takeo ◽

Sergi Sanz ◽

Marcus VG Lacerda ◽

...

Keyword(s):

Escherichia Coli ◽

Plasmodium Vivax ◽

Wheat Germ ◽

Suspension Array

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A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

BioMed Research International ◽

10.1155/2015/678084 ◽

2015 ◽

Vol 2015 ◽

pp. 1-13 ◽

Cited By ~ 29

Author(s):

Cécile Beck ◽

Philippe Desprès ◽

Sylvie Paulous ◽

Jessica Vanhomwegen ◽

Steeve Lowenski ◽

...

Keyword(s):

High Performance ◽

Neurological Diseases ◽

Expression System ◽

Cross Reactivity ◽

Encephalitis Virus ◽

Serological Tests ◽

Multiplex Immunoassay ◽

Tick Borne Encephalitis ◽

Tick Borne Encephalitis Virus ◽

Negative Controls

West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs). These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using theDrosophilaS2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.

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3P221 The endocrine disrupter Bisphenol-A acutely induces morphologica changes in rat hippocampal CA1 pyramidal neurons

Seibutsu Butsuri ◽

10.2142/biophys.44.s245_1 ◽

2004 ◽

Vol 44 (supplement) ◽

pp. S245

Author(s):

N Tanabe ◽

Y Komatsuzaki ◽

T Tsurugizawa ◽

K Mitsuhashi ◽

Y Ooishi ◽

...

Keyword(s):

Bisphenol A ◽

Pyramidal Neurons ◽

Endocrine Disrupter ◽

Ca1 Pyramidal Neurons ◽

Hippocampal Ca1

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Exposition hydrique au bisphénol A et à ses dérivés chlorés et liens avec le cancer du sein : étude de faisabilité BREDI I (Breast and Endocrine Disrupter Investigation Part 1)

Revue des sciences de l eau ◽

10.7202/1034010ar ◽

2015 ◽

Vol 28 (3) ◽

pp. 215-220

Author(s):

Steeve Rouillon ◽

Claire Grignon ◽

Nicolas Venisse ◽

Cédric Nadeau ◽

Marion Albouy-Llaty ◽

...

Keyword(s):

Bisphenol A ◽

Cancer Du Sein ◽

Endocrine Disrupter ◽

Tissu Adipeux ◽

Perturbateurs Endocriniens

Résumé Il existe une relation entre exposition aux perturbateurs endocriniens (PE) et carcinogenèse animale. Cependant, les données épidémiologiques sont insuffisantes. Le bisphénol A (BPA) est un PE ubiquitaire présent dans l’eau potable. Ses dérivés chlorés (Clx-BPA) sont suspectés d’avoir une action PE 100 fois supérieure. L’objectif de ce travail était d’évaluer la faisabilité d’une étude d’exposition hydrique au BPA et aux Clx-BPA dans une population de patientes opérées du sein. L’étude a été menée au CHU de Poitiers auprès de trois populations de femmes opérées du sein classées selon la gravité de leur lésion. Trois façons d’évaluer l’exposition hydrique ont été explorées : dosage dans des matrices biologiques, dans l’eau du robinet et administration d’un questionnaire sociodémographique validé. Le critère de jugement principal était la concentration en composés dans l’eau ou les matrices biologiques et les quantités d’eau apportées par voie orale et cutanée selon le questionnaire. Dans l’eau de boisson analysée, le BPA a été quantifié chez la totalité des patientes (116 ± 162 ng∙L‑1). Les concentrations en Clx-BPA étaient de 1,85 ± 0,70 ng∙L‑1. Les concentrations urinaires en BPA étaient de 2,6 ng∙mL‑1 en préopératoire et 3,8 ± 5,5 ng∙mL‑1 en postopératoire, les Clx-BPA n’ayant pas été quantifiés. Dans le tissu adipeux mammaire, le BPA a été retrouvé à 1,265 ± 0,058 ng∙g‑1, le BPA et le Clx-BPA n’ayant été détectés qu’à deux reprises. Cette étude a montré la faisabilité des dosages du BPA et des Clx-BPA dans les matrices biologiques et l’eau du robinet. La mise en place d’une cohorte multicentrique permettra d’étudier la relation entre exposition à ces PE et cancer du sein.

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A Single Snapshot Multiplex Immunoassay Platform Utilizing Dense Test Lines Based on Engineered Beads

Biosensors and Bioelectronics ◽

10.1016/j.bios.2021.113388 ◽

2021 ◽

pp. 113388

Author(s):

Wonhyung Lee ◽

Hojin Kim ◽

Pan Kee Bae ◽

Sanghyun Lee ◽

Sung Yang ◽

...

Keyword(s):

Multiplex Immunoassay ◽

Single Snapshot

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Abstract 4336: Interrogating the Human Myocardial Interstitium during Myocardial Arrest and Reperfusion: Dynamic Changes in Cytokines and Protease Activity

Circulation ◽

10.1161/circ.118.suppl_18.s_867-a ◽

2008 ◽

Vol 118 (suppl_18) ◽

Author(s):

Robert E Stroud ◽

Christine N Koval ◽

Isabelle Gengler ◽

Anne M Deschamps ◽

John S Ikonomidis ◽

...

Keyword(s):

Detection System ◽

Ischemia Reperfusion ◽

Dynamic Assessment ◽

High Sensitivity ◽

Fluorescent Detection ◽

Suspension Array ◽

Cytokine Analysis ◽

And Function ◽

Inflammatory Molecules ◽

Pulmonary Bypass

Background. Cytokines, such as the interleukins (IL1β, IL2, IL6) and tumor necrosis factor (TNF) can modulate myocardial structure and function with ischemia/reperfusion (I/R) but dynamic assessment of these biological molecules within the human myocardial interstitium with I/R has not been performed, and the inter-relationship to matrix metalloproteinases activity (MMPact) remains unexplored. Accordingly, a fluorogenic microdialysis method was used to simultaneously measure myocardial interstitial cytokine levels and MMPact in patients during and following I/R. Methods . MMPact was measured in patients (n=13) undergoing cardio-pulmonary bypass (CPB) at baseline, during myocardial arrest and CPB (on-CPB), and immediately following reperfusion and separation from CPB (post-CPB) by a validated in-line microdialysis fluorescent detection system. Myocardial interstitial fluid was subjected to cytokine analysis by high sensitivity multiplex suspension array. Results . Interstitial MMPact increased by over 30% post-CPB and was accompanied by a specific change in cytokine profiles (Figure ). The classical pro-inflammatory molecules such as TNF and IL6 were either not detectable or unchanged, whereas IL1β and IL2 which can be proinflammatory, were increased. Conclusions. These unique results demonstrated that a dynamic cytokine signature occurs within the human myocardial interstitium following I/R and is temporally related to heightened MMP activity. Direct interrogation of the human myocardial interstitium may provide a unique insight into critical signaling pathways which may evoke adverse structural and functional events following I/R.

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