Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets

Internal Standard Method, which has been widely used for quantitative analysis of general bio-samples, requires certain skill in sample preparation since it sometimes accompanies a noticeable error due not only to inaccuracy of weight measurement both of a sample and of an internal standard but also to nonuniformity of the internal standard in the target. In this work, a standard-free method of quantitative analysis, based on the fact that the total yield of continuous x-rays from the sample approximately corresponds to effective weight of the sample, has been developed and applied to some typical bio-samples (NIST Bovine liver, a rat liver and a human serum). As a result, values of concentration obtained by the present method present good agreement with those obtained by the internal standard method. Further, in a case where target preparation is performed by unskilled person, the present method gives better results than those obtained by the internal standard method. It is also found that this method is applicable to almost all soft-tissue samples and, moreover, to targets prepared by a different method.

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A solid-phase assay for quantitative analysis of sulfated glycosaminoglycans at the nanogram level. Application to tissue samples

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/s0731-7085(99)00214-9 ◽

1999 ◽

Vol 21 (4) ◽

pp. 859-865 ◽

Cited By ~ 12

Author(s):

D.H Vynios ◽

A Faraos ◽

G Spyracopoulou ◽

A.J Aletras ◽

C.P Tsiganos

Keyword(s):

Quantitative Analysis ◽

Solid Phase ◽

Tissue Samples ◽

Solid Phase Assay ◽

Nanogram Level

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A technique for detection and relative quantitative analysis of glucosephosphate isomerase isozymes from nanogram tissue samples

Biochemical Genetics ◽

10.1007/bf00484725 ◽

1978 ◽

Vol 16 (7-8) ◽

pp. 681-690 ◽

Cited By ~ 31

Author(s):

Alan C. Peterson ◽

Patricia M. Frair ◽

Gordon G. Wong

Keyword(s):

Quantitative Analysis ◽

Glucosephosphate Isomerase ◽

Tissue Samples

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Enteric neuron density correlates with clinical features of severe gut dysmotility

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00199.2019 ◽

2019 ◽

Vol 317 (6) ◽

pp. G793-G801 ◽

Cited By ~ 1

Author(s):

Elisa Boschetti ◽

Carolina Malagelada ◽

Anna Accarino ◽

Juan R. Malagelada ◽

Rosanna F. Cogliandro ◽

...

Keyword(s):

Quantitative Analysis ◽

Clinical Manifestations ◽

Neuron Specific Enolase ◽

Neuronal Cell ◽

Enteric Neurons ◽

Paraffin Sections ◽

Tissue Samples ◽

Neuron Density ◽

Neuronal Cell Bodies ◽

Gi Symptoms

Gastrointestinal (GI) symptoms can originate from severe dysmotility due to enteric neuropathies. Current methods used to demonstrate enteric neuropathies are based mainly on classic qualitative histopathological/immunohistochemical evaluation. This study was designed to identify an objective morphometric method for paraffin-embedded tissue samples to quantify the interganglionic distance between neighboring myenteric ganglia immunoreactive for neuron-specific enolase, as well as the number of myenteric and submucosal neuronal cell bodies/ganglion in jejunal specimens of patients with severe GI dysmotility. Jejunal full-thickness biopsies were collected from 32 patients (22 females; 16–77 yr) with well-characterized severe dysmotility and 8 controls (4 females; 47–73 yr). A symptom questionnaire was filled before surgery. Mann-Whitney U test, Kruskal-Wallis coupled with Dunn’s posttest and nonparametric linear regression tests were used for analyzing morphometric data and clinical correlations, respectively. Compared with controls, patients with severe dysmotility exhibited a significant increase in myenteric interganglionic distance ( P = 0.0005) along with a decrease in the number of myenteric ( P < 0.00001) and submucosal ( P < 0.0004) neurons. A 50% reduction in the number of submucosal and myenteric neurons correlated with an increased interganglionic distance and severity of dysmotility. Our study proposes a relatively simple tool that can be applied for quantitative evaluation of paraffin sections from patients with severe dysmotility. The finding of an increased interganglionic distance may aid diagnosis and limit the direct quantitative analysis of neurons per ganglion in patients with an interganglionic distance within the control range. NEW & NOTEWORTHY Enteric neuropathies are challenging conditions characterized by a severe impairment of gut physiology, including motility. An accurate, unambiguous assessment of enteric neurons provided by quantitative analysis of routine paraffin sections may help to define neuropathy-related gut dysmotility. We showed that patients with severe gut dysmotility exhibited an increased interganglionic distance associated with a decreased number of myenteric and submucosal neurons, which correlated with symptoms and clinical manifestations of deranged intestinal motility.

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