Effects of agmatine on secretion of interferon tau and catecholamines and expression of genes related to production of polyamines by ovine trophectoderm cells

Amino Acids ◽  
2016 ◽  
Vol 48 (10) ◽  
pp. 2389-2399 ◽  
Author(s):  
Yasser Y. Lenis ◽  
Xiaoqiu Wang ◽  
Wanjin Tang ◽  
Guoyao Wu ◽  
Fuller W. Bazer
Keyword(s):  
Interferon Tau ◽  
Expression Of Genes ◽  
Trophectoderm Cells

scholarly journals Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes

Reproduction ◽  
2015 ◽  
Vol 149 (3) ◽  
pp. 281-291 ◽  
Author(s):  
Irene Ruiz-González ◽  
Jing Xu ◽  
Xiaoqiu Wang ◽  
Robert C Burghardt ◽  
Kathrin A Dunlap ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Endogenous Retroviruses ◽  
Dependent Manner ◽  
Innate Immune Responses ◽  
Toll Like Receptors ◽  
Interferon Tau ◽  
Viral Pathogens ◽  
Expression Of Genes ◽  
Trophectoderm Cells ◽  
Pregnancy Recognition

Conceptus–endometrial communication during the peri-implantation period of pregnancy ensures establishment of pregnancy. We hypothesized that this dialog involves exosomes, ovine endogenous jaagsiekte retroviruses (enJSRV) and toll-like receptors (TLR) which regulate the secretion of interferon tau (IFNT), the pregnancy recognition signal in ruminants. First, exosomes isolated from uterine flushings from cyclic and pregnant ewes were analyzed for exosomal content and uterine expression of heat shock protein 70 (HSC70). Then, conceptus trophectoderm cells (oTr1) treated with different doses of exosomes were analyzed for the expression of genes involved in TLR-mediated cell signaling. The results revealed that exosomes contain mRNAs for enJSRV-ENV,HSC70, interleukins, and interferon (IFN)-regulatory factors. Exosomal content of enJSRV-ENVmRNA and protein decreased from days 10 and 12 to day 16 of gestation, and uterine expression of HSC70 increased in pregnant ewes compared with cyclic ewes. The oTr1 cells proliferated and secreted IFNT in a dose-dependent manner in response to exosomes from cyclic ewes. The expression ofCD14,CD68,IRAK1,TRAF6,IRF6,andIRF7mRNAs that are key to TLR-mediated expression of type 1 IFNs was significantly influenced by day of pregnancy. This study demonstrated that exosomes are liberated into the uterine lumen during the estrous cycle and early pregnancy; however, in pregnant ewes, exosomes stimulate trophectoderm cells to proliferate and secrete IFNT coordinately with regulation of TLR-mediated cell signaling. These results support our hypothesis that free and/or exosomal enJSRV act on the trophectoderm via TLR to induce the secretion of IFNT in a manner similar to that for innate immune responses of macrophages and plasmacytoid dendritic cells to viral pathogens.

scholarly journals Trophectoderm-Specific Knockdown of LIN28 Decreases Expression of Genes Necessary for Cell Proliferation and Reduces Elongation of Sheep Conceptus

2020 ◽  
Vol 21 (7) ◽  
pp. 2549 ◽  
Author(s):  
Asghar Ali ◽  
Mark Stenglein ◽  
Thomas Spencer ◽  
Gerrit Bouma ◽  
Russell Anthony ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Critical Period ◽  
In Vitro Experiments ◽  
Placental Development ◽  
Expression Of Genes ◽  
Trophectoderm Cells ◽  
Successful Establishment

LIN28 inhibits let-7 miRNA maturation which prevents cell differentiation and promotes proliferation. We hypothesized that the LIN28-let-7 axis regulates proliferation-associated genes in sheep trophectoderm in vivo. Day 9-hatched sheep blastocysts were incubated with lentiviral particles to deliver shRNA targeting LIN28 specifically to trophectoderm cells. At day 16, conceptus elongation was significantly reduced in LIN28A and LIN28B knockdowns. Let-7 miRNAs were significantly increased and IGF2BP1-3, HMGA1, ARID3B, and c-MYC were decreased in trophectoderm from knockdown conceptuses. Ovine trophoblast (OTR) cells derived from day 16 trophectoderm are a useful tool for in vitro experiments. Surprisingly, LIN28 was significantly reduced and let-7 miRNAs increased after only a few passages of OTR cells, suggesting these passaged cells represent a more differentiated phenotype. To create an OTR cell line more similar to day 16 trophectoderm we overexpressed LIN28A and LIN28B, which significantly decreased let-7 miRNAs and increased IGF2BP1-3, HMGA1, ARID3B, and c-MYC compared to control. This is the first study showing the role of the LIN28-let-7 axis in trophoblast proliferation and conceptus elongation in vivo. These results suggest that reduced LIN28 during early placental development can lead to reduced trophoblast proliferation and sheep conceptus elongation at a critical period for successful establishment of pregnancy.

scholarly journals Role of lipids on elongation of the preimplantation conceptus in ruminants

Reproduction ◽  
2016 ◽  
Vol 152 (4) ◽  
pp. R115-R126 ◽  
Author(s):  
Eduardo S Ribeiro ◽  
José E P Santos ◽  
William W Thatcher
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Cell Biology ◽  
De Novo ◽  
Dynamic Changes ◽  
Expression Of Genes ◽  
Trophectoderm Cells ◽  
Conceptus Development ◽  
Elongation Phase

Elongation of the preimplantation conceptus is a prerequisite for successful pregnancy in ruminants and depends on histotroph secretion by the endometrium. Lipids are an essential component of the histotroph, and recent studies indicate that lipids have important roles in the elongation phase of conceptus development. The onset of elongation is marked by dynamic changes in the transcriptome of trophectoderm cells, which are associated with lipid metabolism. During elongation, the trophectoderm increases transcript expression of genes related to uptake, metabolism andde novobiosynthesis of fatty acids and prostaglandins. Expression of the genePPARGincreases substantially, and activation of the transcription factor PPARG by binding of lipid ligands appears to be crucial for the coordination of cell biology during elongation. Lipids accumulated in the epithelial cells of the endometrium during diestrus are likely the most important source of fatty acids for utilization by the conceptus and become available in the uterine lumen through exporting of exosomes, microvesicles, carrier proteins and lipoproteins. Targeting of uterine lipid metabolism and PPARG activity during preimplantation conceptus development through nutraceutical diets may be a good strategy to improve pregnancy survival and reproductive efficiency in ruminants.

scholarly journals Feeder Cell Type Affects the Growth of In Vitro Cultured Bovine Trophoblast Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Islam M. Saadeldin ◽  
Ahmed Abdelfattah-Hassan ◽  
Ayman Abdel-Aziz Swelum
Keyword(s):  
Granulosa Cells ◽  
Current Approach ◽  
Embryonic Cells ◽  
Interferon Tau ◽  
Porcine Granulosa Cells ◽  
Trophectoderm Cells ◽  
Homeobox Protein ◽  
First Time

Trophectoderm cells are the foremost embryonic cells to differentiate with prospective stem-cell properties. In the current study, we aimed at improving the current approach for trophoblast culture by using granulosa cells as feeders. Porcine granulosa cells (PGCs) compared to the conventional mouse embryonic fibroblasts (MEFs) were used to grow trophectoderm cells from hatched bovine blastocysts. Isolated trophectoderm cells were monitored and displayed characteristic epithelial/cuboidal morphology. The isolated trophectoderm cells expressed mRNA of homeobox protein (CDX2), cytokeratin-8 (KRT8), and interferon tau (IFNT). The expression level was higher on PGCs compared to MEFs throughout the study. In addition, primary trophectoderm cell colonies grew faster on PGCs, with a doubling time of approximately 48 hrs, compared to MEFs. PGCs feeders produced a fair amount of 17β-estradiol and progesterone. We speculated that the supplementation of sex steroids and still-unknown factors during the trophoblasts coculture on PGCs have helped to have better trophectoderm cell’s growth than on MEFs. This is the first time to use PGCs as feeders to culture trophectoderm cells and it proved superior to MEFs. We propose PGCs as alternative feeders for long-term culture of bovine trophectoderm cells. This model will potentially benefit studies on the early trophoblast and embryonic development in bovines.

scholarly journals Effects of catecholamines on secretion of interferon tau and expression of genes for synthesis of polyamines and apoptosis by ovine trophectoderm†

2018 ◽  
Vol 99 (3) ◽  
pp. 611-628 ◽  
Author(s):  
Mohammed A Elmetwally ◽  
Yasser Lenis ◽  
Wanjin Tang ◽  
Guoyao Wu ◽  
Fuller W Bazer
Keyword(s):  
Interferon Tau ◽  
Expression Of Genes

scholarly journals Genes involved in conceptus–endometrial interactions in ruminants: insights from reductionism and thoughts on holistic approaches

Reproduction ◽  
2008 ◽  
Vol 135 (2) ◽  
pp. 165-179 ◽  
Author(s):  
Thomas E Spencer ◽  
Olivier Sandra ◽  
Eckhard Wolf
Keyword(s):  
Pregnancy Loss ◽  
Complex Dynamics ◽  
Transcriptional Profiling ◽  
Placental Lactogen ◽  
Physiological Models ◽  
Holistic Approaches ◽  
Expression Of Genes ◽  
Trophectoderm Cells ◽  
Improve Pregnancy Outcome ◽  
A Cell

This review summarizes new knowledge on expression of genes and provides insights into approaches for study of conceptus–endometrial interactions in ruminants with emphasis on the peri-implantation stage of pregnancy. Conceptus–endometrial interactions in ruminants are complex and involve carefully orchestrated temporal and spatial alterations in gene expression regulated by hormones from the ovary and conceptus. Progesterone is the hormone of pregnancy and acts on the uterus to stimulate blastocyst survival, growth, and development. Inadequate progesterone levels or a delayed rise in progesterone is associated with pregnancy loss. The mononuclear trophectoderm cells of the elongating blastocyst synthesize and secrete interferon-τ (IFNT), the pregnancy recognition signal. Trophoblast giant binucleate cells begin to differentiate and produce hormones including chorionic somatomammotropin 1 (CSH1 or placental lactogen). A number of genes, induced or stimulated by progesterone, IFNT, and/or CSH1 in a cell-specific manner, are implicated in trophectoderm adhesion to the endometrial luminal epithelium and regulation of conceptus growth and differentiation. Transcriptional profiling experiments are beginning to unravel the complex dynamics of conceptus–endometrial interactions in cattle and sheep. Future experiments should incorporate physiological models of pregnancy loss and be complemented by metabolomic studies of uterine lumen contents to more completely define factors required for blastocyst survival, growth, and implantation. Both reduction and holistic approaches will be important to understand the multifactorial phenomenon of recurrent pregnancy loss and provide a basis for new strategies to improve pregnancy outcome and reproductive efficiency in cattle and other domestic animals.

scholarly journals Chronicling the discovery of interferon tau

Reproduction ◽  
2017 ◽  
Vol 154 (5) ◽  
pp. F11-F20 ◽  
Author(s):  
Fuller W Bazer ◽  
William W Thatcher
Keyword(s):  
Placental Lactogen ◽  
Corpora Lutea ◽  
Interferon Tau ◽  
Interferon Stimulated Genes ◽  
Expression Of Genes ◽  
Placental Growth Hormone ◽  
A Cell ◽  
Low Cytotoxicity ◽  
Conditioned Culture Medium ◽  
Uterine Glands

It has been 38 years since a protein, now known as interferon tau (IFNT), was discovered in ovine conceptus-conditioned culture medium. After 1979, purification and testing of native IFNT revealed its unique antiluteolyic activity to prevent the regression of corpora lutea on ovaries of nonpregnant ewes. Antiviral, antiproliferative and immunomodulatory properties of native and recombinant IFNT were demonstrated later. In addition, progesterone and IFNT were found to act cooperatively to silence expression of classical interferon stimulated genes in a cell-specific manner in ovine uterine luminal and superficial glandular epithelia. But, IFNT signaling through a STAT1/STAT2-independent pathway stimulates expression of genes, such as those for transport of glucose and amino acids, which are required for growth and development of the conceptus. Further, undefined mechanisms of action of IFNT are key to a servomechanism that allows ovine placental lactogen and placental growth hormone to affect the development of uterine glands and their expression of genes throughout gestation. IFNT also acts systemically to induce the expression of interferon stimulated genes that influence secretion of progesterone by the corpus luteum. Finally, IFNT has great potential as a therapeutic agent due to its low cytotoxicity, anti-inflammatory properties and effects to mitigate diabetes, obesity-associated syndromes and various autoimmune diseases.

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