scholarly journals Effects of Bisphenol-A on proliferation and expression of genes related to synthesis of polyamines, interferon tau and insulin-like growth factor 2 by ovine trophectoderm cells

2018 ◽  
Vol 78 ◽  
pp. 90-96 ◽  
Author(s):  
Mohammed A. Elmetwally ◽  
Amal A. Halawa ◽  
Yasser Y. Lenis ◽  
Wanjin Tang ◽  
Guoyao Wu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bisphenol A ◽  
Insulin Like Growth Factor ◽  
Interferon Tau ◽  
Expression Of Genes ◽  
Trophectoderm Cells

192 RESVERATROL, A NATURAL FOOD COMPOUND, SUPPRESSED THE CELL GROWTH OF BG-1 OVARIAN CANCER CELLS INDUCED BY 17β-ESTRADIOL OR BISPHENOL A THROUGH DOWNREGULATING ESTROGEN RECEPTOR α AND INSULIN-LIKE GROWTH FACTOR-1 RECEPTOR

2013 ◽  
Vol 25 (1) ◽  
pp. 245
Author(s):  
N.-H. Kang ◽  
K.-C. Choi
Keyword(s):  
Ovarian Cancer ◽  
Growth Factor ◽  
Cell Growth ◽  
Bisphenol A ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cells ◽  
Insulin Like Growth Factor ◽  
Cancer Cell Growth

Resveratrol (trans-3,4,5-trihydroxystilbene; RES) was adopted in this study as a novel phytoestrogen displaying antioxidant, antiinflammatory, and anticancer effects. In this study, we evaluated the inhibitory effect of RES on the cell growth induced by 17β-oestradiol (E2), a typical oestrogen, and bisphenol A (BPA), an endocrine-disrupting chemical (EDC) in BG-1 ovarian cancer cells expressing oestrogen receptors (ER) through down-regulating oestrogen receptor α (ERa) and insulin-like growth factor-1 receptor (IGF-1R). The EDC and oestrogen appear to promote the development of the oestrogen-dependent cancers. Thus, we need to develop therapeutic methods for EDC-dependent cancers. In in vitro experiments, we examined the cell viability and mRNA expression of ERa ± IGF-1R genes following the treatments with E2 or BPA in the presence or absence of RES or ICI 182 780, an ER antagonist, by MTT assay and RT-PCR, respectively. We also examined the protein level of ERa, phosphorylated insulin receptor substrate-1 (IRS-1), phosphorylated Akt1/2/3, p21, and cyclin D1 by Western blot analysis. Treatment with E2 or BPA remarkably increased the growth of BG-1 ovarian cancer cells, and their enhanced cell growth appeared to be mediated by ERa. In addition, the treatment of BG-1 ovarian cancer cells with E2 or BPA resulted in an increase in ERa and IGF-1R gene expressions. However, co-treatment of RES reversed E2- or BPA-induced ovarian cancer cell growth and mRNA expressions of ERa and IGF-1R. The protein levels of phosphorylated IRS-1 and Akt were upregulated by E2 or BPA, whereas these levels were downregulated by co-treatment of RES in the presence of E2 or BPA. Taken together, these results indicate that RES may effectively inhibit ovarian cancer cell growth via downregulating cross-talk between ERa and IGF-1R. This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Ministry of Education, Science and Technology (MEST) of Korea government (no. 2011-0015385).

Effects of agmatine on secretion of interferon tau and catecholamines and expression of genes related to production of polyamines by ovine trophectoderm cells

Amino Acids ◽  
2016 ◽  
Vol 48 (10) ◽  
pp. 2389-2399 ◽  
Author(s):  
Yasser Y. Lenis ◽  
Xiaoqiu Wang ◽  
Wanjin Tang ◽  
Guoyao Wu ◽  
Fuller W. Bazer
Keyword(s):  
Interferon Tau ◽  
Expression Of Genes ◽  
Trophectoderm Cells

scholarly journals Exosomes, endogenous retroviruses and toll-like receptors: pregnancy recognition in ewes

Reproduction ◽  
2015 ◽  
Vol 149 (3) ◽  
pp. 281-291 ◽  
Author(s):  
Irene Ruiz-González ◽  
Jing Xu ◽  
Xiaoqiu Wang ◽  
Robert C Burghardt ◽  
Kathrin A Dunlap ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Endogenous Retroviruses ◽  
Dependent Manner ◽  
Innate Immune Responses ◽  
Toll Like Receptors ◽  
Interferon Tau ◽  
Viral Pathogens ◽  
Expression Of Genes ◽  
Trophectoderm Cells ◽  
Pregnancy Recognition

Conceptus–endometrial communication during the peri-implantation period of pregnancy ensures establishment of pregnancy. We hypothesized that this dialog involves exosomes, ovine endogenous jaagsiekte retroviruses (enJSRV) and toll-like receptors (TLR) which regulate the secretion of interferon tau (IFNT), the pregnancy recognition signal in ruminants. First, exosomes isolated from uterine flushings from cyclic and pregnant ewes were analyzed for exosomal content and uterine expression of heat shock protein 70 (HSC70). Then, conceptus trophectoderm cells (oTr1) treated with different doses of exosomes were analyzed for the expression of genes involved in TLR-mediated cell signaling. The results revealed that exosomes contain mRNAs for enJSRV-ENV,HSC70, interleukins, and interferon (IFN)-regulatory factors. Exosomal content of enJSRV-ENVmRNA and protein decreased from days 10 and 12 to day 16 of gestation, and uterine expression of HSC70 increased in pregnant ewes compared with cyclic ewes. The oTr1 cells proliferated and secreted IFNT in a dose-dependent manner in response to exosomes from cyclic ewes. The expression ofCD14,CD68,IRAK1,TRAF6,IRF6,andIRF7mRNAs that are key to TLR-mediated expression of type 1 IFNs was significantly influenced by day of pregnancy. This study demonstrated that exosomes are liberated into the uterine lumen during the estrous cycle and early pregnancy; however, in pregnant ewes, exosomes stimulate trophectoderm cells to proliferate and secrete IFNT coordinately with regulation of TLR-mediated cell signaling. These results support our hypothesis that free and/or exosomal enJSRV act on the trophectoderm via TLR to induce the secretion of IFNT in a manner similar to that for innate immune responses of macrophages and plasmacytoid dendritic cells to viral pathogens.

Increasing metabolisable energy and protein supplementation to stimulate the subsequent milk production during late gestation by increasing proliferation and reducing apoptosis in goat mammary gland prepartum

2019 ◽  
Vol 59 (10) ◽  
pp. 1820
Author(s):  
F. Shabrandi ◽  
E. Dirandeh ◽  
Z. Ansari-Pirsaraei ◽  
A. Teimouri-Yansari
Keyword(s):  
Mammary Gland ◽  
Growth Factor ◽  
Milk Production ◽  
Feed Intake ◽  
Late Gestation ◽  
Insulin Like Growth Factor ◽  
Early Lactation ◽  
Expression Of Genes ◽  
Metabolisable Energy ◽  
Igfbp 3

In total, 32 pregnant goats were assigned randomly to four diets fed from Day 100 of pregnancy to Day 30 after parturition, to determine the effects of metabolisable energy (ME) and metabolisable protein (MP) supplementation levels on feed intake, subsequent colostrum and milk production and expression of genes regulating mammary-cell proliferation and apoptosis. Diets were as follows: (1) diet with ME and MP provided according to NRC recommendations (control), (2) diet with extra 10% ME, (3) diet with extra 10% MP, and (4) diet 1 with 10% extra of both ME and MP. Mammary biopsies were obtained from each udder half 24 h after parturition. Feed intake (g/day), and colostrum (kg/day) and milk (kg/month) production increased when the extra ME and MP were provided together prepartum and in early lactation (P < 0.05). Relative mRNA expressions significantly increased in the mammary gland of insulin-like growth factor 1 (IGF-1, 4.3-fold), IGF-1 receptor (IGF-1R, 3.6-fold) and B-cell lymphoma 2 (Bcl-2, 4.6-fold), whereas insulin-like growth factor binding protein 3 (IGFBP-3, 3.2-fold), Bcl-2-associated X protein (Bax, 16.7-fold) and the ratio of Bax:Bcl-2 expressions significantly decreased (69.8-fold) with increased ME and MP levels fed in late gestation. In conclusion, colostrum production and milk yield in the early lactation period are sensitive to nutrient supply during gestation, where increased dietary ME as well as MP supplementation levels during late gestation will favour mammary development, by increasing expression of genes stimulating cellular proliferation (IGF-1, IGF-1R, Bcl-2) and reduced those stimulating apoptosis (IGFBP-3, Bax).

scholarly journals 243TEMPORAL DIVERGENCE IN THE PATTERN OF MRNA EXPRESSION IN BOVINE EMBRYOS CULTURED FROM THE ZYGOTE TO BLASTOCYST STAGE IN VITRO OR IN VIVO

2004 ◽  
Vol 16 (2) ◽  
pp. 242
Author(s):  
P. Lonergan ◽  
D. Rizos ◽  
A. Gutierrez-Adan ◽  
P.M. Moreira ◽  
B. Pintado ◽  
...  
Keyword(s):  
Growth Factor ◽  
Relative Abundance ◽  
Transcript Abundance ◽  
Blastocyst Stage ◽  
Insulin Like Growth Factor ◽  
Bovine Embryos ◽  
Interferon Tau ◽  
Cell Stage

The objective of this study was to examine the time during the post-fertilization culture period that gene expression patterns of in vitro cultured bovine embryos diverge from those of their in vivo cultured counterparts. Presumptive bovine zygotes were produced by IVM/IVF of immature oocytes collected from the ovaries of slaughtered animals. At approximately 20h post-insemination (hpi), presumptive zygotes were randomly divided into two culture groups, either in vitro in synthetic oviduct fluid or in vivo, and transferred into the ewe oviduct. Embryos were recovered from both systems at approximately 30hpi (2-cell), two (4-cell), three (8-cell), four (16-cell), five (early morula), six (compact morula) or seven (blastocyst) days pi and snap-frozen for the analysis of transcript abundance using real-time PCR. The transcripts studied were interferon-tau, apoptosis regulator box-a (Bax), connexin 43, sarcosine oxidase, glucose transporter 5, mitochondrial Mn-superoxide dismutase, insulin-like growth factor II, and insulin-like growth factor-I receptor, most of which are known from our previous work to be differentially transcribed in blastocysts derived from culture in vitro or in vivo. Analysis was done on pools of 10 embryos. Data were analyzed using one-way repeated measures ANOVA. The relative abundance of the transcripts studied varied throughout the preimplantation period and was strongly influenced by the culture environment. For example, transcripts for interferon-tau were detected from the 8-cell stage onwards in in vitro-cultured embryos but not until the early morula stage in those cultured in vivo. Levels of this transcript increased significantly at the compact morula and blastocyst stages in both groups but were significantly higher (P<0.05) in in vitro-cultured embryos at both stages. mRNA for Bax was not detected before the 8-cell stage in in vitro cultured embryos and not until the 16-cell stage in in vivo cultured embryos. The abundance of this transcript increased significantly thereafter up to the blastocyst stage in both groups. The level of expression was significantly higher (P<0.05) at all stages of development in in vitro-cultured embryos than those cultured in vivo. The relative abundance of Cx43 transcripts decreased in both in vitro- and in vivo-cultured embryos at the 8- to 16-cell stage. Levels remained low thereafter in the in vitro-cultured embryos but significantly increased in those cultured in vivo. Transcript abundance was significantly higher in in vivo cultured embryos from Day 4 onwards with a ten-fold difference presence at the blastocyst stage. Differences also existed for the other transcripts studied. These data demonstrate that changes in transcript abundance in blastocyst stage embryos are in many cases a consequence of perturbed transcription earlier in development. Depending on the transcript, these differences may be evident in as short as 10h of culture.

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