scholarly journals Effect of microbial sanitizers for reducing biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on stainless steel by cultivation with UHT milk

2018 ◽  
Vol 28 (1) ◽  
pp. 289-296 ◽  
Author(s):  
Nutthawut Meesilp ◽  
Nutthisha Mesil
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Uht Milk

scholarly journals Chemical sanitizers to control biofilms formed by two Pseudomonas species on stainless steel surface

2012 ◽  
Vol 32 (1) ◽  
pp. 142-150 ◽  
Author(s):  
Danila Soares Caixeta ◽  
Thiago Henrique Scarpa ◽  
Danilo Florisvaldo Brugnera ◽  
Dieyckson Osvani Freire ◽  
Eduardo Alves ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stainless Steel ◽  
Biofilm Formation ◽  
Skim Milk ◽  
304 Stainless Steel ◽  
Stainless Steel Surface ◽  
Aisi 304 ◽  
Pseudomonas Species ◽  
Sodium Dichloroisocyanurate ◽  
Different Temperatures

The biofilm formation of Pseudomonas aeruginosa and Pseudomonas fluorescens on AISI 304 stainless steel in the presence of reconstituted skim milk under different temperatures was conducted, and the potential of three chemical sanitizers in removing the mono-species biofilms formed was compared. Pseudomonas aeruginosa cultivated in skim milk at 28 °C presented better growth rate (10.4 log CFU.mL-1) when compared with 3.7 and 4.2 log CFU.mL-1 for P. aeruginosa and P. fluorescens cultivated at 7 °C, respectively. Pseudomonas aeruginosa formed biofilm when cultivated at 28 °C. However, only the adhesion of P. aeruginosa and P. fluorescens was observed when incubated at 7 °C. The sodium dichloroisocyanurate was the most efficient sanitizer in the reduction of the adhered P. aeruginosa cells at 7 and 28 °C and those on the biofilm, respectively. The hydrogen peroxide was more effective in the reduction of adhered cells of P. fluorescens at 7 °C.

scholarly journals BIOFILM FORMATION OF STAPHYLOCOCCUS AUREUS, SALMONELLA ENTERITIDIS AND PSEUDOMONAS AERUGINOSA IN PRESENCE OF N-BENZOTIAZOL-2-YL-BENZENSULFONAMIDE DERIVATIVES

2010 ◽  
Vol 0 (3(11)) ◽  
pp. 32-40
Author(s):  
I. О. Малярчик ◽  
Т. О. Філіпова ◽  
Т. О. Філіпова ◽  
Б. М. Галкін ◽  
Б. М. Галкін
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Salmonella Enteritidis

scholarly journals Resistance of nosocomial strains to antibacterial drugs and its link to biofilm formation

2017 ◽  
Vol 8 (4) ◽  
pp. 540-546
Author(s):  
T. V. Sklyar ◽  
K. V. Lavrentievа ◽  
Y. A. Alyonkina ◽  
A. M. Kolomoets ◽  
А. І. Vinnikov
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Clinical Practice ◽  
Biofilm Formation ◽  
Direct Relationship ◽  
Wide Distribution ◽  
Clinical Isolates ◽  
Diffusion Test ◽  
Antibacterial Drugs ◽  
Agar Diffusion Test

The problem of nosocomial infections is considered in connection with more frequent formation and wide distribution in clinical practice of new strains of hospital bacteria that have a cross-resistence to antibacterial drugs. The nosocomial agents were isolated from wounds and identified as Staphylococcus aureus and Pseudomonas aeruginosa. 72.0% of S. aureus strains and 61.5% of P. aeruginosa clinical isolates had the capability of forming biofilms. The sensitivity to antibiotics of all isolated strains was investigated with tne agar diffusion test. This method showed that all strains of S. aureus with the capability to form biofilms had resistence to erythromycin, gentamycin, ciprofloxacin and levofloxacin. The had the greatest sensitivity to klindamycin (90.3%), vancomycin (80.6%) and gatifloxacin (80.6% cultures). The strains of S. aureus with the capability to form biofilms were more resistent to antibiotics than strains of S. aureus without such properties. Only cefotaxim suppressed the growth of 75.0% of strains of staphylococci. All isolated strains of S. aureus without the capability to form biofilms were sensitive to doxycyclin, gentamycin, ciprofloxacin, levofloxacin and klindamycin. All clinical isolates of P. aeruginosa with capability to form biofilms had resistence to ampicillin, gentamycin, imipenem, cefotaxime and ceftriaxone. They were most sensitive (75.0%) to piperacillin and cefoperazone/sulbactam. The strains of P. aeruginosa without the capability to form biofilms kept the resistence to gentamycin, imipenem and ceftriaxone. They showed the greatest sensitivity (75.0%) to ciprofloxacin (80.0% isolates) and also to amikacin, ampicillin, meropenem, norfloxacin and cefotaxime (60.0% cultures). We investigated the minimum inhibitory concentrations of gentamycin and ciprofloxacin, which appeared higher for P. aeruginosa than for S. aureus. The most effective disinfectant against all isolated nosocomial agents without the capacity for biofilm formation was “Desactin” in a concentration 0.1% or 0.2%. For strains of staphylococci with this capability, the efficiency of “Desactin” went down by 9.7%. The best biocide effect against the strains of P. aeruginosa with the capability of forming biofilms was shown by 0.1% solution of “Neochlorine tabs”, which suppressed the growth of 75.0% of tested cultures. As a result, we detected a direct relationship between resistance to antibiotics and disinfectants and the capacities for biofilm formation among the nosocomial agents S. aureus and P. aeruginosa. 

scholarly journals Antibacterial and Antibiofilm Potential of Sea Anemone (Stichodactyla haddoni)-Isolated Vibrio (V. parahaemolyticus and V. alginolyticus), and Pseudoalteromonas (P. gelatinilytica and P. piscicida) Against Staphylococcus aureus and Pseudomonas aeruginosa

2021 ◽  
Vol 25 (1) ◽  
Author(s):  
Neda Fazeli ◽  
Akram Sadat Naeemi ◽  
Seyed Amir Hossein Jalali ◽  
Hojjatollah Zamani
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Sea Anemone ◽  
Broth Culture ◽  
Antibiofilm Activity ◽  
Microtiter Plates ◽  
Protease Enzyme ◽  
Molecular Identification Methods ◽  
Stichodactyla Haddoni

Background: Staphylococcus aureus and Pseudomonas aeruginosa are important human bacterial pathogens, which are resistant to several antibiotics. One of the main causes of their resistance is the ability of biofilm formation. Objectives: The present study aimed to evaluate the antibacterial and antibiofilm activity of the extracts of Vibrio parahaemolyticus, V. alginolyticus, Pseudoalteromonas gelatinilytica, and Pseudoalteromonas piscicida isolated from sea anemone (Stichodactyla haddoni) against S. aureus and P. aeruginosa. Methods: Four isolated bacteria were identified using biochemical and molecular identification methods, and their extracts were obtained by mixing the cell-free supernatants from their old broth culture using ethyl acetate and methanol as the solvents. The agar well-diffusion and micro-dilution methods were also applied to determine the antibacterial activity, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) of the extracts. The ability of the extracts to inhibit biofilm formation and disrupt the preformed biofilm of the pathogens was attained through crystal violet staining in 96-well microtiter plates. To determine the nature of the extracts, they were exposed to protease enzyme, and the antibiofilm activity was compared with the untreated extracts. Results: The extracts of the four isolated bacteria inhibited bacterial growth and biofilm formation and disrupted the preformed biofilm of S. aureus (MIC = BIC = 600 µg/mL) and P. aeruginosa (MIC = BIC = 300 µg/mL). In addition, the active compounds of the extracts with antibiofilm activities were mainly proteases. Conclusions: According to the results, V. parahaemolyticus, V. alginolyticus, P. gelatinilytica, and P. piscicida had antibacterial and antibiofilm potential against S. aureus and P. aeruginosa, and their extract could also be further analyzed as an alternative to antibiotics.

scholarly journals Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms by Pseudomonas aeruginosa and Staphylococcus aureus

2009 ◽  
Vol 75 (11) ◽  
pp. 3586-3592 ◽  
Author(s):  
Phat L. Tran ◽  
Adrienne A. Hammond ◽  
Thomas Mosley ◽  
Janette Cortez ◽  
Tracy Gray ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Bacterial Attachment ◽  
Wound Dressings ◽  
Aqueous Environment ◽  
Burn Victims ◽  
Surgical Wounds

ABSTRACT Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphate-buffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.

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