Stress relaxation of swine growth plate in semi-confined compression: depth dependent tissue deformational behavior versus extracellular matrix composition and collagen fiber organization

2012 ◽  
Vol 12 (1) ◽  
pp. 67-78 ◽  
Author(s):  
Samira Amini ◽  
Farhad Mortazavi ◽  
Jun Sun ◽  
Martin Levesque ◽  
Caroline D. Hoemann ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stress Relaxation ◽  
Growth Plate ◽  
Collagen Fiber ◽  
Matrix Composition ◽  
Confined Compression ◽  
Compression Depth ◽  
Deformational Behavior ◽  
Fiber Organization

Matrix composition modulates vitamin D3’s effects on 3D collagen fiber organization by MCF10A cells

2021 ◽  
Author(s):  
Nafis Hasan ◽  
Yang Zhang ◽  
Irene Georgakoudi ◽  
Carlos Sonnenschein ◽  
Ana M. Soto
Keyword(s):  
Vitamin D3 ◽  
Collagen Fiber ◽  
Matrix Composition ◽  
3D Collagen ◽  
Mcf10a Cells ◽  
Fiber Organization

scholarly journals Cardiac Fibroblasts and the Extracellular Matrix in Regenerative and Nonregenerative Hearts

2019 ◽  
Vol 6 (3) ◽  
pp. 29 ◽  
Author(s):  
Luis Hortells ◽  
Anne Katrine Z. Johansen ◽  
Katherine E. Yutzey
Keyword(s):  
Extracellular Matrix ◽  
Collagen Fiber ◽  
Cellular Proliferation ◽  
Cardiac Fibroblasts ◽  
Postnatal Period ◽  
Cardiac Fibroblast ◽  
Scar Formation ◽  
Cardiac Cells ◽  
Fiber Organization

During the postnatal period in mammals, the heart undergoes significant remodeling and cardiac cells progressively lose their embryonic characteristics. At the same time, notable changes in the extracellular matrix (ECM) composition occur with a reduction in the components considered facilitators of cellular proliferation, including fibronectin and periostin, and an increase in collagen fiber organization. Not much is known about the postnatal cardiac fibroblast which is responsible for producing the majority of the ECM, but during the days after birth, mammalian hearts can regenerate after injury with only a transient scar formation. This phenomenon has also been described in adult urodeles and teleosts, but relatively little is known about their cardiac fibroblasts or ECM composition. Here, we review the pre-existing knowledge about cardiac fibroblasts and the ECM during the postnatal period in mammals as well as in regenerative environments.

scholarly journals Navigating the Collagen Jungle: The Biomedical Potential of Fiber Organization in Cancer

2021 ◽  
Vol 8 (2) ◽  
pp. 17
Author(s):  
Jonathan N. Ouellette ◽  
Cole R. Drifka ◽  
Kelli B. Pointer ◽  
Yuming Liu ◽  
Tyler J Lieberthal ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Collagen Fiber ◽  
Disease Stage ◽  
Fibrillar Collagen ◽  
Multiple Cancer ◽  
Clinical Evaluations ◽  
Standard Clinical Practice ◽  
Cancer Types ◽  
Biomedical Potential ◽  
Fiber Organization

Recent research has highlighted the importance of key tumor microenvironment features, notably the collagen-rich extracellular matrix (ECM) in characterizing tumor invasion and progression. This led to great interest from both basic researchers and clinicians, including pathologists, to include collagen fiber evaluation as part of the investigation of cancer development and progression. Fibrillar collagen is the most abundant in the normal extracellular matrix, and was revealed to be upregulated in many cancers. Recent studies suggested an emerging theme across multiple cancer types in which specific collagen fiber organization patterns differ between benign and malignant tissue and also appear to be associated with disease stage, prognosis, treatment response, and other clinical features. There is great potential for developing image-based collagen fiber biomarkers for clinical applications, but its adoption in standard clinical practice is dependent on further translational and clinical evaluations. Here, we offer a comprehensive review of the current literature of fibrillar collagen structure and organization as a candidate cancer biomarker, and new perspectives on the challenges and next steps for researchers and clinicians seeking to exploit this information in biomedical research and clinical workflows.

Influence of the rotating magnetic field on bacterial biofilm extracellular matrix composition

2020 ◽  
Author(s):  
Radosław DRozd ◽  
Magdalena Szymańska ◽  
Kuba Hoppe ◽  
Adam Junka ◽  
Karol Fijałkowski
Keyword(s):  
Magnetic Field ◽  
Extracellular Matrix ◽  
Bacterial Biofilm ◽  
Rotating Magnetic Field ◽  
Matrix Composition

India Ink Pinprick Experiments on Surface Organization of Cricoarytenoid Joints

1986 ◽  
Vol 29 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Joel C. Kahane ◽  
Alice R. Kahn
Keyword(s):  
Articular Cartilage ◽  
Collagen Fiber ◽  
Collagen Fibers ◽  
Articular Surfaces ◽  
India Ink ◽  
Age Related ◽  
Cricoarytenoid Joint ◽  
Mechanical Factors ◽  
Fiber Organization

Collagen fiber organization in the articular surfaces of the cricoarytenoid joint (CAJ) was studied using a pinpricking technique used in biomechanical research in orthopedics. Four male human formalin preserved specimens (3 months to 20 years) and 6 male freshly autopsied specimens (19 to 30 yrs) were studied. Specimens were dissected using the stereomicroscope. Distinctive patterns of articular cartilage slits reflect the orientation of collagen fibers in the cricoid and arytenoid articular surfaces. The orientation of the collagen fibers reinforces the articular surfaces along the principle path of CAJ motion. No age related differences were found. This suggests that the orientation of collagen fibers in the CAJ articular surfaces is prenatally determined rather than significantly influenced by postnatal mechanical factors.

scholarly journals Multiscale molecular profiling of pathological bone resolves sexually dimorphic control of extracellular matrix composition

2021 ◽  
Vol 14 (3) ◽  
pp. dmm048116 ◽  
Author(s):  
Aikta Sharma ◽  
Alice Goring ◽  
Peter B. Johnson ◽  
Roger J. H. Emery ◽  
Eric Hesse ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Collagen Fibre ◽  
Matrix Composition ◽  
Label Free ◽  
Fibre Number ◽  
Backscattered Electron ◽  
Matrix Mineralisation

ABSTRACTCollagen assembly during development is essential for successful matrix mineralisation, which determines bone quality and mechanocompetence. However, the biochemical and structural perturbations that drive pathological skeletal collagen configuration remain unclear. Deletion of vascular endothelial growth factor (VEGF; also known as VEGFA) in bone-forming osteoblasts (OBs) induces sex-specific alterations in extracellular matrix (ECM) conformation and mineralisation coupled to vascular changes, which are augmented in males. Whether this phenotypic dimorphism arises as a result of the divergent control of ECM composition and its subsequent arrangement is unknown and is the focus of this study. Herein, we used murine osteocalcin-specific Vegf knockout (OcnVEGFKO) and performed ex vivo multiscale analysis at the tibiofibular junction of both sexes. Label-free and non-destructive polarisation-resolved second-harmonic generation (p-SHG) microscopy revealed a reduction in collagen fibre number in males following the loss of VEGF, complemented by observable defects in matrix organisation by backscattered electron scanning electron microscopy. This was accompanied by localised divergence in collagen orientation, determined by p-SHG anisotropy measurements, as a result of OcnVEGFKO. Raman spectroscopy confirmed that the effect on collagen was linked to molecular dimorphic VEGF effects on collagen-specific proline and hydroxyproline, and collagen intra-strand stability, in addition to matrix carbonation and mineralisation. Vegf deletion in male and female murine OB cultures in vitro further highlighted divergence in genes regulating local ECM structure, including Adamts2, Spp1, Mmp9 and Lama1. Our results demonstrate the utility of macromolecular imaging and spectroscopic modalities for the detection of collagen arrangement and ECM composition in pathological bone. Linking the sex-specific genetic regulators to matrix signatures could be important for treatment of dimorphic bone disorders that clinically manifest in pathological nano- and macro-level disorganisation.This article has an associated First Person interview with the first author of the paper.

scholarly journals Effect of Metformin on Cardiac Metabolism and Longevity in Aged Female Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Xudong Zhu ◽  
Weiyan Shen ◽  
Zhu Liu ◽  
Shihao Sheng ◽  
Wei Xiong ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cardiac Metabolism ◽  
Metabolic Reprogramming ◽  
Matrix Composition ◽  
Metformin Treatment ◽  
Related Gene ◽  
Atp Production ◽  
Female Mice ◽  
Multiple Organs ◽  
Inflammatory Status

The antidiabetic drug metformin exerts pleiotropic effects on multiple organs, including the cardiovascular system. Evidence has shown that metformin improves healthspan and lifespan in male mice, yet its lifespan lengthening effect in females remains elusive. We herein demonstrated that metformin fails to extend the lifespan in female mice. Compared to 2-month-old young controls, 20-month-old female mice showed a spectrum of degenerative cardiac phenotypes alongside significant alterations in the extracellular matrix composition. Despite lowered reactive oxygen species production, long-term metformin treatment did not improve cardiac function in the aged female mice. In contrast, RNA sequencing analyses demonstrated that metformin treatment elevated the extracellular matrix-related gene while lowering oxidative phosphorylation-related gene expression in the heart. In addition, metformin treatment induced metabolic reprogramming that suppressed mitochondrial respiration but activated glycolysis (i.e., Warburg effect) in cultured primary cardiomyocytes and macrophages, thereby sustaining an inflammatory status and lowering ATP production. These findings suggest the unexpected detrimental effects of metformin on the regulation of cardiac homeostasis and longevity in female mice, reinforcing the significance of comprehensive testing prior to the translation of metformin-based novel therapies.

scholarly journals Plasmid-encoded H-NS controls extracellular matrix composition in a modern Acinetobacter baumannii urinary isolate

2021 ◽  
Author(s):  
Saida Benomar ◽  
Gisela Di Venanzio ◽  
Mario F. Feldman
Keyword(s):  
Extracellular Matrix ◽  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Gene Cluster ◽  
Biofilm Formation ◽  
Therapeutic Potential ◽  
Matrix Composition ◽  
Type Vi Secretion System ◽  
Gram Negative ◽  
Conjugative Plasmids

Acinetobacter baumannii is emerging as a multidrug-resistant (MDR) nosocomial pathogen of increasing threat to human health worldwide. The recent MDR urinary isolate UPAB1 carries the plasmid pAB5, a member of a family of large conjugative plasmids (LCP). LCP encode several antibiotic resistance genes and repress the type VI secretion system (T6SS) to enable their dissemination, employing two TetR transcriptional regulators. Furthermore, pAB5 controls the expression of additional chromosomally encoded genes, impacting UPAB1 virulence. Here we show that a pAB5-encoded H-NS transcriptional regulator represses the synthesis of the exopolysaccharide PNAG and the expression of a previously uncharacterized three-gene cluster that encodes a protein belonging to the CsgG/HfaB family. Members of this protein family are involved in amyloid or polysaccharide formation in other species. Deletion of the CsgG homolog abrogated PNAG production and CUP pili formation, resulting in a subsequent reduction in biofilm formation. Although this gene cluster is widely distributed in Gram-negative bacteria, it remains largely uninvestigated. Our results illustrate the complex cross-talks that take place between plasmids and the chromosomes of their bacterial host, which in this case can contribute to the pathogenesis of Acinetobacter . IMPORTANCE The opportunistic human pathogen Acinetobacter baumannii displays the highest reported rates of multidrug resistance among Gram-negative pathogens. Many A. baumannii strains carry large conjugative plasmids like pAB5. In recent years, we have witnessed an increase in knowledge about the regulatory cross-talks between plasmids and bacterial chromosomes. Here we show that pAB5 controls the composition of the bacterial extracellular matrix, resulting in a drastic reduction in biofilm formation. The association between biofilm formation, virulence, and antibiotic resistance is well-documented. Therefore, understanding the factors involved in the regulation of biofilm formation in Acinetobacter has remarkable therapeutic potential.

Influence of tumorangiogenesis on extracellular matrix composition in malignant gliomas

1997 ◽  
Vol 99 ◽  
pp. S28
Author(s):  
Christel Herold-Mende ◽  
Daniela Riede ◽  
Margareta M. Mueller ◽  
Annette Buttler ◽  
Hans-Herbert Steiner ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Malignant Gliomas ◽  
Matrix Composition
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