The ability of different irrigation methods to remove mixtures of calcium hydroxide and barium sulphate from isthmuses in 3D printed transparent root canal models

Diseases with the highest burden for society such as stroke, myocardial infarction, pulmonary embolism, and others are due to blood clots. Preclinical and clinical techniques to study blood clots are important tools for translational research of new diagnostic and therapeutic modalities that target blood clots. In this study, we employed a three-dimensional (3D) printed middle cerebral artery model to image clots under flow conditions using preclinical imaging techniques including fluorescent whole-body imaging, magnetic resonance imaging (MRI), and computed X-ray microtomography (microCT). Both liposome-based, fibrin-targeted, and non-targeted contrast agents were proven to provide a sufficient signal for clot imaging within the model under flow conditions. The application of the model for clot targeting studies and thrombolytic studies using preclinical imaging techniques is shown here. For the first time, a novel method of thrombus labeling utilizing barium sulphate (Micropaque®) is presented here as an example of successfully employed contrast agents for in vitro experiments evaluating the time-course of thrombolysis and thus the efficacy of a thrombolytic drug, recombinant tissue plasminogen activator (rtPA). Finally, the proof-of-concept of in vivo clot imaging in a middle cerebral artery occlusion (MCAO) rat model using barium sulphate-labelled clots is presented, confirming the great potential of such an approach to make experiments comparable between in vitro and in vivo models, finally leading to a reduction in animals needed.

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Effect of double antibiotic and calcium hydroxide pastes on dislodgement resistance of an epoxy resin-based and two calcium silicate-based root canal sealers

Clinical Oral Investigations ◽

10.1007/s00784-016-1877-1 ◽

2016 ◽

Vol 21 (4) ◽

pp. 1277-1282 ◽

Cited By ~ 11

Author(s):

Hakan Gokturk ◽

Emre Bayram ◽

Huda Melike Bayram ◽

Tugrul Aslan ◽

Yakup Ustun

Keyword(s):

Epoxy Resin ◽

Calcium Hydroxide ◽

Root Canal ◽

Calcium Silicate ◽

Root Canal Sealers

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Portland Cement Use in Dental Root Perforations: A Long Term Followup

Case Reports in Dentistry ◽

10.1155/2014/637693 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5

Author(s):

Álvaro Henrique Borges ◽

Matheus Coelho Bandeca ◽

Mateus Rodrigues Tonetto ◽

Luis Augusto Faitaroni ◽

Elibel Reginna de Siqueira Carvalho ◽

...

Keyword(s):

Portland Cement ◽

Calcium Hydroxide ◽

Root Canal ◽

Clinical Signs ◽

Signs And Symptoms ◽

Clinical Aspects ◽

Iatrogenic Perforation ◽

Root Canals ◽

First Case ◽

Clinical Signs And Symptoms

Root canal and furcal perforations are causes of endodontic therapy failure and different materials that stimulate tissue mineralization have been proposed for perforation treatment. In the first case, a patient presented tooth 46 with unsatisfactory endodontic treatment and a periapical radiographic lesion. A radiolucent area compatible with a perforating internal resorption cavity was found in the mesial root. The granulation tissue was removed, and root canals were prepared. The intracanal medication was composed of calcium hydroxide and the perforation cavity was filled with Portland cement. The 11-year followup showed radiographic repair of the tissue adjacent to the perforation and absence of clinical signs and symptoms or periapical lesion. In the second case, a patient presented with edema on the buccal surface of tooth 46. The examination showed a radiolucent area in the furcation region compatible with an iatrogenic perforation cavity. The mesial root canals were calcified, and only the distal root canal was prepared. The cavity was filled with a calcium hydroxide-based paste and the distal root canal was obturated. In sequence, the perforation cavity was filled with Portland cement. The 9-year followup showed the tooth in masticatory function with radiographic and clinical aspects compatible with normality.

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In vitro evaluation of the effectiveness of the chemomechanical preparation against Enterococcus faecalis after single- or multiple-visit root canal treatment

Brazilian Oral Research ◽

10.1590/s1806-83242007000400005 ◽

2007 ◽

Vol 21 (4) ◽

pp. 308-313 ◽

Cited By ~ 8

Author(s):

Eduardo Diogo Gurgel-Filho ◽

Nilton Vivacqua-Gomes ◽

Brenda Paula Figueiredo de Almeida Gomes ◽

Caio Cezar Randi Ferraz ◽

Alexandre Augusto Zaia ◽

...

Keyword(s):

Calcium Hydroxide ◽

Enterococcus Faecalis ◽

Root Canal ◽

Control Groups ◽

Single Visit ◽

Root Canals ◽

Multiple Visit ◽

Microbial Samples ◽

Chlorhexidine Gel

The purpose was to assess the elimination of Enterococcus faecalis in vitro in human mandibular premolars after chemomechanical preparation with or without the use of a calcium hydroxide dressing. After 60 days of contamination with E. faecalis, the root canals were prepared using the Crown-Down technique combined with 2% chlorhexidine gel irrigation. Then, the specimens were divided into two experimental groups, treated in a single visit or in multiple visits, and two control groups. The multiple-visit group received a dressing with calcium hydroxide for 14 days (CalenTM) and the single-visit group did not receive any medication. In the two control groups, the canals were filled with BHI after chemomechanical preparation with 2% chlorhexidine gel or distilled water. Microbial samples were taken from the root canals for colony forming unit count for each phase of the treatment using sterile paper points inside the root canal lumen. Data were ranked and analyzed by the Kruskal-Wallis statistical test. The residual microbial colonies were then assessed. The results showed that chemomechanical preparation using 2% chlorhexidine gel with no intra-canal dressing reduced by 100% the E. faecalis contamination of the root canal lumen. The calcium-hydroxide group that received the 14-day intra-canal dressing allowed a small number of bacteria to grow between visits, but without statistical differences between groups.

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Dentinal pH changes following electrophoretically activated calcium hydroxide ions in the root canal space of bovine teeth

Dental Traumatology ◽

10.1111/j.1600-9657.2005.00308.x ◽

2005 ◽

Vol 21 (3) ◽

pp. 146-149 ◽

Cited By ~ 4

Author(s):

Igor Tsesis ◽

Shaul Lin ◽

Ervin I. Weiss ◽

Zvi Fuss

Keyword(s):

Calcium Hydroxide ◽

Root Canal ◽

Ph Changes

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Passive ultrasonic irrigation in calcium hydroxide removal from root canals: a sem/eds analysis

Journal of Research in Dentistry ◽

10.19177/jrd.v3e32015668-677 ◽

2015 ◽

Vol 3 (3) ◽

pp. 668

Author(s):

Fernanda Hoffmann Busanello ◽

Angela Longo Do Nascimento ◽

Lilian Tietz ◽

Mirela Sangoi Barreto ◽

Ricardo Abreu Da Rosa ◽

...

Keyword(s):

Calcium Hydroxide ◽

Root Canal ◽

Control Group ◽

Passive Ultrasonic Irrigation ◽

Root Canals ◽

Eds Analysis ◽

Ultrasonic Irrigation ◽

Calcium Hydroxide Removal ◽

And Control ◽

Elementary Chemical

AIM: The aim of this study was to evaluate the efficacy of different lengths of time of passive ultrasonic irrigation (PUI) in removing calcium hydroxide (CH) paste from root canal, using scanning electron microscopy and energy dispersive spectrometry (SEM/EDS). MATERIAL AND METHODS: Fifty-four human premolars were used. After coronal access, a size #15 K-file was used to obtain apical patency. Root canal preparation was performed using WaveOne 40.08 instruments. CH paste was placed into the root canal. Specimens were stored in a humid environment, and after seven days, they were divided into five groups (n=12) according to the irrigation protocols: Manual– a size #40 K-file; PUI/1– 1 min; PUI/2– 2 min; PUI/3– 3 min; and Control– without CH paste. An elementary chemical microanalysis (SEM/EDS) was performed to quantify the presence of calcium on the dentinal walls. RESULTS: The percentage of calcium was higher in all experimental groups when compared to the control group (P < 0.05); but no differences among them were found (P > 0.05). CONCLUSION: The amount of calcium hydroxide paste on the dentinal walls was not dependent on length of time of ultrasonic activation. SEM/EDS analysis seems to be a reliable method to assess CH paste removal from the root canals.

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Sealability of Different Root Canal Nanosealers: Nano Calcium Hydroxide and Nano Bioactive Glass

The Open Dentistry Journal ◽

10.2174/1874210601913010308 ◽

2019 ◽

Vol 13 (1) ◽

pp. 308-315

Author(s):

Asmaa A. Desouky ◽

Maged M. Negm ◽

Magdy M. Ali

Keyword(s):

Zinc Oxide ◽

Bioactive Glass ◽

Calcium Hydroxide ◽

Root Canal ◽

Blue Dye ◽

Nano Powder ◽

Zinc Oxide Eugenol ◽

Nail Polish ◽

Stereo Microscope

Background: The success of the endodontic treatment is largely dependent on the sealing achieved by root canal obturation. The application of sealer fills imperfections and increases adaptation of the root filling to the canal walls. Aim: To evaluate the sealability of experimental nanosealers (nano calcium hydroxide and nano bioactive glass) and to compare it with the commercial zinc oxide eugenol sealer using a dye penetration method. Materials and Methods: Sixty single-rooted mandibular premolars were selected. The tooth crowns were removed so as to obtain standardized 15-mm-long root specimens. The root canal was instrumented with Protaper Ni-Ti rotary file and the final file size was up to # F4/.06 (in vitro study). They were then randomly allocated into 3 groups of 20 specimens each (n=20) according to the sealer used for obturation, and all samples were filled with single cone gutta-percha (#40/06) and one of the tested sealers. All teeth were coated with nail polish and then suspended in 2% methylene blue dye for 7 days. Stereo-microscope (x10) was used to evaluate the sealability of newly introduced nanosealers. The data were statistically analyzed by ANOVA test followed by post hoc analysis (P < 0.05). Results: Significant improvement shown by the presented study suggests that nano calcium hydroxide sealer showed significantly less dye leakage than nano bioactive glass sealer and zinc oxide eugenol sealer. Conclusion: The results of this study showed that the synthesized nano-powder sealers are suitable for use in root canal therapy to prevent leakage. The root canal can be sealed better by using smaller nano-powder particle sizes. In addition, the two groups exhibited significant differences in leakage in comparison with commonly used ZOE sealer.

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Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1061 ◽

2011 ◽

Vol 12 (5) ◽

pp. 368-371 ◽

Cited By ~ 3

Author(s):

Matheus Melo Pithon ◽

Deyla Duarte Vilela ◽

Manoel Matos Neto ◽

Alexandre Mascarenhas Villela

Keyword(s):

Calcium Hydroxide ◽

Clinical Significance ◽

Root Canal ◽

Canal System ◽

Root Canals ◽

Anterior Teeth ◽

Knowing That ◽

Group I ◽

Canal Systems ◽

Root Canal System

ABSTRACT Aim To evaluate the interference of the intracanal medication Calen® (SSWhite, São Paulo, Brazil) on the filling of simulated lateral canals. Materials and methods Twenty human anterior teeth were used. Before the endodontic filling procedures the access of cavity was made, and after this root canals were made in all the teeth to simulate the presence of lateral canals. After preparation, the teeth were randomly divided into two groups (n=10). In group I, the root canal system was filled directly after chemicalmechanical preparation; in group II, endodontic treatment was performed in multiple sessions, and after preparation the calcium hydroxide-based intracanal medication Calen® was inserted. After the period of 7 days, the root canals were vigorously irrigated and then they were filled. Next, the teeth were radiographed to verify the quality of the filling. Results The results demonstrated that the teeth treated in a single session, without calcium hydroxide medication, presented 47 canals out of 60 with radiographic evidence of filling, whereas the teeth in which intracanal medication was used, only 07 presented a radiographic image compatible with filling (p < 0.05). Conclusion The use of the calcium hydroxide-based medication Calen made it difficult to obtain a hermetic filling of the root canal system. Clinical significance The clinical significance of this work basing on the fact that once the dentist knowing that property obliteration of calcium hydroxide can be taken care when they are used in the presence of lateral canals. How to cite this article Vilela DD, Neto MM, Villela AM, Pithon MM. Evaluation of Interference of Calcium Hydroxide-based Intracanal Medication in Filling Root Canal Systems . J Contemp Dent Pract 2011;12(5):368-371.

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