Transcriptomic and metabolic responses of Staphylococcus aureus in mixed culture with Lactobacillus plantarum, Streptococcus thermophilus and Enterococcus durans in milk

2016 ◽  
Vol 43 (9) ◽  
pp. 1237-1247 ◽  
Author(s):  
Kamila Zdenkova ◽  
Babek Alibayov ◽  
Ludmila Karamonova ◽  
Sabina Purkrtova ◽  
Renata Karpiskova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mixed Culture ◽  
Lactobacillus Plantarum ◽  
Streptococcus Thermophilus ◽  
Metabolic Responses ◽  
Enterococcus Durans

scholarly journals EFFECT OF EXOPOLISACCHARIDES OF LACTIC ACID BACTERIA ON THE SYNTHESIS OF PROINFLAMMATORY CYTOKINES BY MACROPHAGIC MICE IN PHAGOCYTOSIS OF STAPHYLOCOCCUS AUREUS

2018 ◽  
pp. 67-71
Author(s):  
G. T. Uryadova ◽  
E. A. Gorelnikova ◽  
N. A. Fokina ◽  
A. S. Dolmashkina ◽  
L. V. Karpunina
Keyword(s):  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactococcus Lactis ◽  
Inflammatory Cytokines ◽  
Proinflammatory Cytokines ◽  
Streptococcus Thermophilus ◽  
Ambiguous Effect ◽  
Pro Inflammatory Cytokines

Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 13, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals.

scholarly journals Utilização de bactérias ácido-lácticas isoladas do trato intestinal de tilápia-do-nilo como probiótico

2008 ◽  
Vol 43 (9) ◽  
pp. 1201-1207 ◽  
Author(s):  
Adolfo Jatobá ◽  
Felipe do Nascimento Vieira ◽  
Celso Buglione Neto ◽  
Bruno Corrêa Silva ◽  
José Luís Pedreira Mouriño ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Lactobacillus Brevis ◽  
Enterococcus Durans

O objetivo deste trabalho foi isolar bactérias ácido-lácticas do intestino de tilápias-do-nilo, e avaliar seu potencial probiótico. Foram isoladas cepas de bactérias ácido-lácticas, e foi avaliada a inibição aos patógenos in vitro. As cepas com os melhores resultados foram identificadas e utilizadas no experimento de colonização do trato intestinal de tilápias-do-nilo, via suplementação na dieta, em delineamento inteiramente ao acaso, com três tratamentos e quatro repetições. Foram avaliados: o total de bactérias, as bactérias ácido-lácticas, Vibrio ssp. e Pseudomonas ssp. A cepa com melhor resultado foi utilizada na infecção experimental, em delineamento inteiramente ao acaso, em esquema fatorial 2x3: dieta suplementada com a cepa e dieta-controle; e os peixes não submetidos à injeção, peixes submetidos à injeção de solução salina e à injeção de Enterococcus durans, com três repetições. Foram avaliados os parâmetros hematológicos. As duas cepas identificadas foram: Lactobacillus plantarum e Lactobacillus brevis, que colonizaram o trato intestinal de tilápias, contudo L. plantarum teve menor número total de bactérias e de Pseudomonas ssp. Foi observado maior número total de eritrócitos, trombócitos, leucócitos, linfócitos, neutrófilos e monócitos, em peixes alimentados com L. plantarum e submetidos à injeção de E. durans. O L. plantarum tem efeito probiótico e melhora o sistema imune das tilápias.

Antibacterial Activity of Dental Restorative Materials

1971 ◽  
Vol 50 (2) ◽  
pp. 382-387 ◽  
Author(s):  
David M. Updegraff ◽  
Robert W.H. Chang ◽  
Richard W. Joos
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Lactobacillus Plantarum ◽  
Bacteriostatic Activity ◽  
Dental Restorative Materials ◽  
Restorative Materials ◽  
Mixed Bacteria ◽  
Dental Restorative

Twenty-one commercial dental restorative materials, aged under various conditions, were checked on two different culture mediums for their antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, a cariogenic streptococcus, and mixed bacteria from the mouth. The duration of bacteriostatic activity was decreased rapidly by leaching in water. Of the three acrylics checked for ability to prevent in vitro carieslike lesions, the acrylic with highest antibacterial activity had the least caries.

Thermograms ofStreptococcus thermophilusandLactobacillus bulgaricusin single and mixed culture in milk medium

1979 ◽  
Vol 46 (3) ◽  
pp. 485-496 ◽  
Author(s):  
Paul R. Monk
Keyword(s):  
Mixed Culture ◽  
Streptococcus Thermophilus ◽  
Ph Control ◽  
Lactobacillus Bulgaricus ◽  
Stimulation Of

SummaryMicrobial thermograms have been used to investigate the growth ofStreptococcus thermophilusandLactobacillus bulgaricusin single and mixed culture in milk medium. Growth has been observed with different ratios of the strains in the inoculum and, at one ratio, the effect of the amount of inoculum. The effect of serial sub-culture both with and without pH control has been observed, and stimulation ofStr. thermophilusby cell-free fitrates ofL. bulgaricushas been examined. The development of the technique is discussed in terms of the application of microbial thermograms in selecting cultures for incorporation into mixtures and evaluating their performance.

scholarly journals SIFAT ANTAGONISTIK Lactobacillus sp B441 DAN II442 ASAL TEMPOYAK TERHADAP Staphylococcus aureus (Antagonism of Lactobacillus sp B441 and II442 from Tempoyak against Staphylococcus aureus)

2015 ◽  
Vol 34 (04) ◽  
pp. 430 ◽  
Author(s):  
Tri Wardani Widowati ◽  
Basuni Hamzah ◽  
Agus Wijaya ◽  
Rindit Pambayun
Keyword(s):  
Staphylococcus Aureus ◽  
Risk Factor ◽  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Pathogenic Bacteria ◽  
Natural Habitat ◽  
Bactericidal Effect ◽  
Initial Population ◽  
Test Kit ◽  
Mucous Membranes

Lactobacillus sp B441 and II442 were species of lactic acid bacteria isolated from tempoyak, a kind of food fermented made from durian flesh. Staphylococcus aureus is a pathogenic bacteria that has natural habitat on the surface of human body such as on the skin and in mucous membranes like the nose. Contamination of Staphylococcus aureuswas a risk factor in tempoyak fermentation, especially, during taking the durian flesh from seeds. The aim of this research was to observe antagonistic property of Lactobacillus sp B441 and II442 isolated from tempoyak during low temperature fermentation against Staphylococcus aureus. Phenotypic identification using API 50 CHL test kit indicatedthat isolates were Lactobacullus plantarum B441 and Lactobacillus plantarum II442. Based on co-culture analysis show that isolates of Lactobacillus plantarum B441 and II442 have bactericidal effect against Staphylococcus aureus ATCC 25923 as a indicator of pathogenic bacteria. At 25 th hours, population of Staphylococcus aureus ATCC 25923 in mix-culture with Lactobacillus plantarum B441 and Lactobacillus plantarum II442 decreased 3 – 4 log cycle from initial population. However, population of Staphylococcus aureus ATCC 25923 in mono-culture increased 2 log cycles at 25 th hours. Keywords: Lactobacillus sp, tempoyak, antagonism, Staphylococcus aureus ABSTRAKLactobacillus sp B441 dan II442 merupakan spesies bakteri asam laktat yang diisolasi dari tempoyak yaitu makanan fermentasi dari daging buah durian. Staphylococcus aureus adalah bakteri patogenik yang habitat alaminya pada permukaan kulit manusia dan membran mukosa seperti hidung. Proses fermentasi tempoyak khususnya saat pemisahandaging buah durian dari bijinya beresiko terkontaminasi oleh Staphylococcus aureus. Tujuan dari penelitian ini adalah mengkaji sifat antagonistik dari Lactobacillus sp B441 and II442 yang diisolasi dari tempoyak terhadap Staphylococcus aureus. Identifikasi secara fenotif menggunakan API 50 CHL test kit menunjukkan bahwa isolat bakteri asam laktat tersebut adalah Lactobacullus plantarum B441 dan Lactobacillus plantarum II442. Berdasar uji co-culture diketahuibahwa kedua spesies tersebut mempunyai efek bakterisidal terhadap bakteri Staphylococcus aureus ATCC 25923. Pada jam ke 25 Staphylococcus aureus yang ditumbuhkan secara mix-culture dengan Lactobacillus plantarum B441 dan Lactobacillus plantarum II442 asal tempoyak turun 3 – 4 log cycle dari populasi awal. Sedangkan populasiStaphylococcus aureus ATCC 25923 secara mono-culture meningkat sekitar 2 log cycle pada jam ke 25.Kata kunci: Lactobacillus sp, tempoyak, antaginistik, Staphylococcus aureus

Sign in / Sign up

Export Citation Format

Share Document