Heterologous expression of Fusarium oxysporum tomatinase in Saccharomyces cerevisiae increases its resistance to saponins and improves ethanol production during the fermentation of Agave tequilana Weber var. azul and Agave salmiana must

Abstract The DOM34 gene of Saccharomyces cerevisiae is similar togenes found in diverse eukaryotes and archaebacteria. Analysis of dom34 strains shows that progression through the G1 phase of the cell cycle is delayed, mutant cells enter meiosis aberrantly, and their ability to form pseudohyphae is significantly diminished. RPS30A, which encodes ribosomal protein S30, was identified in a screen for high-copy suppressors of the dom34Δ growth defect. dom34Δ mutants display an altered polyribosome profile that is rescued by expression of RPS30A. Taken together, these data indicate that Dom34p functions in protein translation to promote G1 progression and differentiation. A Drosophila homolog of Dom34p, pelota, is required for the proper coordination of meiosis and spermatogenesis. Heterologous expression of pelota in dom34Δ mutants restores wild-type growth and differentiation, suggesting conservation of function between the eukaryotic members of the gene family.

Download Full-text

Ethanol Production from Concentrated Food Waste Hydrolyzates Using Free and Immobilized Yeast Saccharomyces cerevisiae H058

Advance Journal of Food Science and Technology ◽

10.19026/ajfst.12.2830 ◽

2016 ◽

Vol 12 (1) ◽

pp. 15-26

Author(s):

Shoubao Yan ◽

Cuie Shi ◽

Shunchang Wang ◽

Kegui Zhang ◽

Qianqian Tong

Keyword(s):

Saccharomyces Cerevisiae ◽

Ethanol Production ◽

Food Waste ◽

Immobilized Yeast

Download Full-text

Effects of Ultrasound on Fermentation of Glucose to Ethanol by Saccharomyces cerevisiae

Fermentation ◽

10.3390/fermentation5010016 ◽

2019 ◽

Vol 5 (1) ◽

pp. 16 ◽

Cited By ~ 5

Author(s):

Luis Huezo ◽

Ajay Shah ◽

Frederick Michel

Keyword(s):

Saccharomyces Cerevisiae ◽

Mass Transfer ◽

Glucose Uptake ◽

Ethanol Production ◽

Ethanol Yield ◽

Biofuel Production ◽

Inhibitory Effects ◽

Negative Effects ◽

Intraparticle Mass Transfer ◽

Improve Mass Transfer

Previous studies have shown that pretreatment of corn slurries using ultrasound improves starch release and ethanol yield during biofuel production. However, studies on its effects on the mass transfer of substrates and products during fermentation have shown that it can have both beneficial and inhibitory effects. In this study, the effects of ultrasound on mass transfer limitations during fermentation were examined. Calculation of the external and intraparticle observable moduli under a range of conditions indicate that no external or intraparticle mass transfer limitations should exist for the mass transfer of glucose, ethanol, or carbon dioxide. Fermentations of glucose to ethanol using Saccharomyces cerevisiae were conducted at different ultrasound intensities to examine its effects on glucose uptake, ethanol production, and yeast population and viability. Four treatments were compared: direct ultrasound at intensities of 23 and 32 W/L, indirect ultrasound (1.4 W/L), and no-ultrasound. Direct and indirect ultrasound had negative effects on yeast performance and viability, and reduced the rates of glucose uptake and ethanol production. These results indicate that ultrasound during fermentation, at the levels applied, is inhibitory and not expected to improve mass transfer limitations.

Download Full-text