Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues

2019 ◽  
Vol 166 (4) ◽  
pp. 512-516 ◽  
Author(s):  
S. V. Nikulin ◽  
T. N. Gerasimenko ◽  
S. A. Shilin ◽  
G. S. Zakharova ◽  
I. N. Gazizov ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
In Vitro Models ◽  
Barrier Tissues

Comparative label-free monitoring of immunotoxin efficacy in 2D and 3D mamma carcinoma in vitro models by impedance spectroscopy

2014 ◽  
Vol 53 ◽  
pp. 370-376 ◽  
Author(s):  
Sarah Poenick ◽  
Heinz-Georg Jahnke ◽  
Marie Eichler ◽  
Stefan Frost ◽  
Hauke Lilie ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
In Vitro Models ◽  
Label Free ◽  
Mamma Carcinoma ◽  
2D And 3D

Use of Impedance Spectroscopy to Assess the Effect of Laminins on the in vitro Differentiation of Intestinal Cells

2019 ◽  
Vol 35 (6) ◽  
pp. 102-107
Author(s):  
E.N. Knyazev ◽  
D.V. Maltseva ◽  
D.A. Sakharov ◽  
T.N. Gerasimenko
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Monolayer ◽  
Electrical Parameters ◽  
Russian Science ◽  
Electric Capacitance ◽  
Cell Growth And Differentiation ◽  
Degree Of Differentiation ◽  
Barrier Tissues ◽  
Laminin 332

The comparative analysis of electrical parameters of a Caco-2 cell monolayer cultured on substrates coated with laminins 111,332и511 has been carried out. The cell electrical parameters reflect its state and, in particular, its degree of differentiation. Impedance spectroscopy is the most convenient method for comprehensive assessment of electrical parameters. It was shown via this method that laminin 332 was the optimal substrate for CACO-2 cell growth and differentiation in vitro. The use of laminin 511 led to significantly lower electric capacitance, and laminin 111 caused a large increase in TEER. impedance spectroscopy, laminins, barrier tissues, TEER, electric capacitance, extracellular matrix, Caco-2 The study was funded by the Russian Science Foundation (Project 16-19-10597).

Measurement of EGTA-Mediated Barrier Impairment by Impedance Spectroscopy

2021 ◽  
Vol 37 (1) ◽  
pp. 95-100
Author(s):  
A.I. Osipyants ◽  
D.M. Hushpulyan ◽  
D.A. Sakharov
Keyword(s):  
Impedance Spectroscopy ◽  
Intestinal Barrier ◽  
In Vitro Models ◽  
The State ◽  
Strong Increase ◽  
High Temporal Resolution ◽  
Electrical Capacitance ◽  
Russian Science ◽  
Detailed Kinetics

For the widespread use of in vitro models of barrier tissues in practice, it is necessary to develop approaches to the automation of cultivation and monitoring of the state of cells. Impedance spectroscopy is a promising and informative method for assessing the state of cell barriers. In this work, the dynamics of electrical parameters in an in vitro model of the intestinal barrier with a high temporal resolution has been studied using impedance spectroscopy. For the first time, the detailed kinetics of changes in TEER, electrical capacitance and additional resistance of a monolayer of Caco-2 cells at various EGTA concentrations was recorded. It was demonstrated that spontaneous restoration of the integrity of the cell barrier is possible in the presence of 2 mM EGTA in the medium, which begins approximately 6 h after the compound addition. A strong increase in TEER was also found immediately after the addition of EGTA, which probably depended on the EGTA concentration and can be explained by a decrease in intracellular conductance due to the closure of transmembrane channels. impedance spectroscopy, intestine, TEER, electrical capacitance, EGTA The study was funded by the Russian Science Foundation (project 16-19-10597).

Analysis and applicability of different in vitro models of Glioblastoma multiforme

2014 ◽  
Vol 226 (06) ◽  
Author(s):  
D William ◽  
M Linnebacher ◽  
CF Classen
Keyword(s):  
Glioblastoma Multiforme ◽  
In Vitro Models

Effect of carbon source on Phenobarbital metabolism by Rhizopus stolonifer

2010 ◽  
Vol 3 (2) ◽  
pp. 1022-1027
Author(s):  
Kavitha K ◽  
Asha S ◽  
Hima Bindu T.V.L ◽  
Vidyavathi M
Keyword(s):  
Carbon Source ◽  
High Performance ◽  
Carbon Sources ◽  
In Vitro Models ◽  
Rhizopus Stolonifer ◽  
Safety And Efficacy ◽  
Alternative Systems ◽  
Toxicological Studies ◽  
Microbial Model

The safety and efficacy of a drug is based on its metabolism or metabolite formed. The metabolism of drugs can be studied by different in vitro models, among which microbial model became popular. In the present study, eight microbes were screened for their ability to metabolize phenobarbital in a manner comparable to humans with a model to develop alternative systems to study human drug metabolism. Among the different microbes screened, a filamentous fungi Rhizopus stolonifer metabolized phenobarbital to its metabolite which is used for further pharmacological and toxicological studies. The transformation of phenobarbital was identified by high- performance liquid chromatography (HPLC). Interestingly, Rhizopus stolonifer sample showed an extra metabolite peak at 3.11min. compared to its controls. The influence of different carbon sources in media used for growth of fungus, on metabolite production was studied, to find its effect in production of metabolite as the carbon source may influence the growth of the cell.

Analysis of the Dynamics of the Electric Capacitance of a Cell Monolayer at the Late Stages of Caco-2 cell Differentiation

2019 ◽  
Vol 35 (6) ◽  
pp. 87-90
Author(s):  
S.V. Nikulin ◽  
V.A. Petrov ◽  
D.A. Sakharov
Keyword(s):  
Impedance Spectroscopy ◽  
Cell Monolayer ◽  
Microfluidic Chips ◽  
Russian Science ◽  
Electric Capacitance ◽  
A Cell ◽  
Static Conditions ◽  
Late Stages

The real-time monitoring of electric capacitance (impedance spectroscopy) allowed obtaining evidence that structures which look like intestinal villi can be formed during the cultivation under static conditions as well as during the cultivation in microfluidic chips. It was shown in this work via transcriptome analysis that the Hh signaling pathway is involved in the formation of villus-like structures in vitro, which was previously shown for their formation in vivo. impedance spectroscopy, intestine, villi, electric capacitance, Hh The study was funded by the Russian Science Foundation (Project 16-19-10597).

3D Culture Modelling: An Emerging Approach for Translational Cancer Research in Sarcomas

2020 ◽  
Vol 27 (29) ◽  
pp. 4778-4788 ◽  
Author(s):  
Victoria Heredia-Soto ◽  
Andrés Redondo ◽  
José Juan Pozo Kreilinger ◽  
Virginia Martínez-Marín ◽  
Alberto Berjón ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Cancer Biology ◽  
Soft Tissues ◽  
Three Dimensional ◽  
3D Culture ◽  
Resistance Mechanisms ◽  
In Vitro Models ◽  
Tumour Spheroids ◽  
Current Therapies

Sarcomas are tumours of mesenchymal origin, which can arise in bone or soft tissues. They are rare but frequently quite aggressive and with a poor outcome. New approaches are needed to characterise these tumours and their resistance mechanisms to current therapies, responsible for tumour recurrence and treatment failure. This review is focused on the potential of three-dimensional (3D) in vitro models, including multicellular tumour spheroids (MCTS) and organoids, and the latest data about their utility for the study on important properties for tumour development. The use of spheroids as a particularly valuable alternative for compound high throughput screening (HTS) in different areas of cancer biology is also discussed, which enables the identification of new therapeutic opportunities in commonly resistant tumours.

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