Backscattered Scanning Electron Microscopy Approach for Assessment of Microvessels under Conditions of Normal Microanatomy and Pathological Neovascularization

2020 ◽  
Vol 169 (4) ◽  
pp. 525-530 ◽  
Author(s):  
R. A. Mukhamadiyarov ◽  
A. G. Kutikhin
Metals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 2
Author(s):  
Víctor Beltrán ◽  
Benjamín Weber ◽  
Ricardo Lillo ◽  
María-Cristina Manzanares ◽  
Cristina Sanzana ◽  
...  

The purpose of this study is to assess the use of grade V titanium mini transitional implants (MTIs) immediately loaded by a temporary overdenture. For this, a histomorphometric analysis of the bone area fraction occupancy (BAFO) was performed by backscattered scanning electron microscopy (BS-SEM). Four female patients were submitted to surgery in which two MTIs were installed and immediately loaded with a temporary acrylic prosthesis. During the same surgery, two regular diameter implants were placed inside the bone and maintained without mechanical load. After 8 months, the MTIs were extracted using a trephine and processed for ultrastructural bone analysis by BS-SEM, and the regular-diameter implants were loaded with an overdenture device. A total of 243 BAFOs of MTIs were analyzed, of which 94 were mainly filled with cortical bone, while 149 were mainly filled with trabecular bone. Bone tissue analysis considering the total BAFOs with calcified tissues showed 72.13% lamellar bone, 26.04% woven bone, and 1.82% chondroid bone without significant differences between the samples. This study revealed that grade V titanium used in immediately loaded MTI was successfully osseointegrated by a mature and vascularized bone tissue as assessed from the BAFO.


1985 ◽  
Vol 22 (12) ◽  
pp. 1930-1944 ◽  
Author(s):  
Tylon O. Willingham ◽  
Bartholomew Nagy ◽  
Lois Anne Nagy ◽  
David H. Krinsley ◽  
David J. Mossman

The Elliot Lake – Blind River, Ontario, paleoplacer deposits in the basal Matineda Formation, lowermost member of the 2.25–2.45 Ga old Huronian Supergroup, contain organic matter chemically consistent with kerogen. This substance is also referred to as thucholite. Uranium ores and some gold occur here, and these minerals may be in close association with the kerogen. Two uraniferous and auriferous stratiform kerogens, obtained from the Denison Mines Limited's Denison mine and Rio Algom Limited's Stanleigh mine, have been analyzed by combined high-vacuum pyrolysis – gas chromatography – mass spectrometry and by neutron activation. The reflectances of these samples have also been determined. Related samples containing dispersed kerogen have been examined by backscattered scanning electron microscopy. The polymer-like matrix of the two stratiform kerogens consists of aromatic, alkyl aromatic hydrocarbon, and sulphur moieties and contains 20 and 32% uranium with gold abundances in the parts per billion range. The reflectances of the two stratiform kerogens are generally higher than those of the dispersed kerogens; the atomic H/C ratios of the former are −0.6 and −0.4. Backscattered scanning electron microscopy and petrographic observations reveal a complex diagenetic history. Stratigraphic position and supportive analytical data suggest that the stratiform kerogens were probably derived from ancient mats of cyanobacteria, subjected to various radiation-induced reactions, and, at least in part, were affected in a manner similar to the surrounding rocks. The latter experienced physical and chemical diagenetic reactions, which often caused repeated mineral fracturing and led to the local development of authigenic carbonates and feldspar. Some of the chemical nature and history of the stratiform kerogens resemble those of the Witwatersrand carbon seam kerogens.


2011 ◽  
Vol 194-196 ◽  
pp. 1012-1016
Author(s):  
Xin Yu Zhang ◽  
Emmanuel Gallucci ◽  
Karen Scrivener

Alkali aggregate reaction (AAR) is known leading to great damage in concrete, both in compressive strength and in durability. In this study, a backscattered scanning electron microscopy with energy dispersive spectroscopy is adopted to diagnose AAR reaction. The results show that the type of AAR reaction and reaction products can be easily identified and the method is precise and fast.


2021 ◽  
Vol 20 (3) ◽  
pp. 2742
Author(s):  
R. A. Mukhamadiyarov ◽  
A. G. Kutikhin

Aim. Using an original method based on backscattered scanning electron microscopy, to study the structural features of the mitral valve leaflets in infective endocarditis.Material and methods. We examined 9 mitral valves extracted during surgical interventions due to structural malfunction in patients with infective endocarditis (IE). The samples were fixed in buffered paraformaldehyde with osmium tetraoxide postfixation. After dehydration by increasing alcohol concentration and acetone, the samples were placed in epoxy resin. After the resin has polymerized, the samples were ground and then polished to the desired depth. To increase the electronic contrast, the samples were treated with a uranyl acetate alcohol solution during dehydration and with Reynolds' lead citrate after polishing the epoxy blocks. The samples were visualized by backscattered scanning electron microscopy at an accelerating 15-kV voltage.Results. Structural leaflet injuries caused by IE were most pronounced in the central part and the base. Necrotic areas were extensive electron-dense formations located in the central leaflet layers, or displaced towards the ventricular surface. The electron-dense material in the necrotic area was poorly structured and contained individual cells and bacteria. Bacteria were also present outside the necrotic area. Necrotic areas were surrounded by a layer of a modified extracellular matrix, usually covered with a fibrin layer. Among the extracellular matrix fibers, the macrophages, smooth myocytes and fibroblasts was noted. The fibrin layer, in addition to these cells, contained a large number of blood vessels and was often covered with endothelium.Conclusion. Infection of the mitral valve leaflets causes a simultaneous inflammatory response and regeneration activation. Without adequate regulatory factors, the processes of inflammation and connective tissue creation lead to structural and functional leaflet failure. Specific causes may be overgrowth of necrotic and inflammatory areas, edema and fiber orientation disorder, as well as leaflet rupture.


Sign in / Sign up

Export Citation Format

Share Document