Direct and protective effects of single or combined addition of vincristine and ε-viniferin on human HepG2 cellular oxidative stress markers in vitro

The aim of this study was to evaluate the protective effects of hydroethanolic extracts of S. scabrum and C. verticillata against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of C. verticillata/S. scabrum at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of S. scabrum and C. verticillata can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.

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Ochratoxin A presence in Cabernet Sauvignon wine changes antioxidant activity in vitro and oxidative stress markers in vivo

Food Additives & Contaminants Part A ◽

10.1080/19440049.2020.1802067 ◽

2020 ◽

Vol 37 (10) ◽

pp. 1755-1764

Author(s):

Luana Schmidt ◽

Natália de Vargas Heck ◽

Ingrid Ferreira ◽

Gabriela Göethel ◽

Sabrina Somacal ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Ochratoxin A ◽

Cabernet Sauvignon ◽

Oxidative Stress Markers ◽

Stress Markers ◽

And Oxidative Stress

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THE PROTECTIVE EFFECTS OF ECHINOPS HETEROPHYLLUS AQUEOUS EXTRACT AGAINST METHOTREXATE-INDUCED HEPATOTOXICITY IN RABBITS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i1.30213 ◽

2019 ◽

Vol 12 (1) ◽

pp. 384 ◽

Cited By ~ 1

Author(s):

Heba Abdulmohsin ◽

Ahmed Abu Raghif ◽

Mohammed Jabbar Manna

Keyword(s):

Oxidative Stress ◽

Liver Damage ◽

Crude Extract ◽

Negative Control ◽

Control Group ◽

Protective Effects ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Study Results ◽

Flavonoid Fraction

Objective: The aim of this study was to investigate antioxidant and hepatoprotective properties of Iraqi Echinops heterophyllus aqueous crude extract and its flavonoid fraction against methotrexate (MTX)-induced hepatotoxicity in rabbits.Methods: MTX-induced hepatotoxicity by administration of 20 mg/kg MTX intraperitoneally for 3 successive days was used as animal model, and animals were arrayed in four groups with eight animals in each group: Group 1 was the healthy control, Group 2 - the negative control receiving MTX only, Group 3 received MTX+crude extract of E. heterophyllus, and Group 4 administered MTX+flavonoid fraction of E. heterophyllus. The study duration was 10 days; at day 11, animals were sacrificed, and the blood samples were obtained for the measurement of serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total bilirubin, total protein, and albumin as well as ELISA assay of the oxidative stress markers such as glutathione (GSH) and malondialdehyde (MDA). The liver was dissected and processed for histopathological investigation and scoring. Statistical analysis was performed to investigate the significance of each result.Results: The study results revealed severe liver damage due to MTX administration in the negative control (induced-non treated) group in comparison with healthy group, also there was significant hepatoprotective effect after administration of the crude extract of E. heterophyllus, and flavonoid fraction from E. heterophyllus shown after biochemical liver function tests and anti-oxidant properties demonstrated by the measurement of oxidative stress markers MDA and GSH. The crude extract of E. heterophyllus shown superior hepatoprotective and antioxidant effect. Histopathological scoring showed a remarkable decrease in the scores of the treatment groups in comparison with the high score in the MTX only treated group.Conclusions: MTX administered in a dose of 20 mg/kg for 3 successive days causes marked liver injury, while treatment with the crude extract and flavonoid fraction of E. heterophyllus significantly ameliorates MTX-induced liver damage, although the crude extract of E. heterophyllus seems to have the most hepatoprotective properties.

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Ferulic acid attenuates osteoporosis induced by glucocorticoid through regulating the GSK-3β/Lrp-5/ERK signalling pathways

Physiology International ◽

10.1556/2060.2021.00180 ◽

2021 ◽

Author(s):

Wei Zhou ◽

Bo Chen ◽

Jingbo Shang ◽

Renbo Li

Keyword(s):

Oxidative Stress ◽

Ferulic Acid ◽

Saline Control ◽

Control Group ◽

Oxidative Stress Markers ◽

Expression Ratio ◽

Stress Markers ◽

Osteogenic Markers ◽

Gsk 3Β

Abstract Objective To evaluate in-vivo and in-vitro effects of ferulic acid (FA) on glucocorticoid-induced osteoarthritis (GIO) to establish its possible underlying mechanisms. Methods The effects of FA on cell proliferation, cell viability (MTT assay), ALP activity, and mineralization assay, and oxidative stress markers (ROS, SOD, GSH LDH and MDA levels) were investigated by MC3T3-E1 cell line. Wistar rats received standard saline (control group) or dexamethasone (GC, 2 mg−1 kg) or DEX+FA (50 and 100 mg−1 kg) orally for 8 weeks. Bone density, micro-architecture, bio-mechanics, bone turnover markers and histo-morphology were determined. The expression of OPG, RANKL, osteogenic markers, and other signalling proteins was assessed employing quantitative RT-PCR and Western blotting. Results The findings indicated the elevation of ALP mRNA expressions, osteogenic markers (Runx-2, OSX, Col-I, and OSN), and the β-Catenin, Lrp-5 and GSK-3β protein expressions. FA showed the potential to increase MC3T3-E1 cell differentiation, proliferation, and mineralization. FA increased oxidative stress markers (SOD, MDA, and GSH) while decreasing ROS levels and lactate dehydrogenase release in GIO rats. The OPG/RANKL mRNA expression ratio was increased by FA, followed by improved GSK-3β and ERK phosphorylation with enhanced mRNA expressions of Lrp-5 and β-catenin. Conclusion These findings showed that FA improved osteoblasts proliferation with oxidative stress suppression by controlling the Lrp-5/GSK-3β/ERK pathway in GIO, demonstrating the potential pathways involved in the mechanism of actions of FA in GIO therapy.

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Irvingia gabonensis Seed Extract: An Effective Attenuator of Doxorubicin-Mediated Cardiotoxicity in Wistar Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/1602816 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Olufunke Olorundare ◽

Adejuwon Adeneye ◽

Akinyele Akinsola ◽

Phillip Kolo ◽

Olalekan Agede ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Wistar Rats ◽

Troponin I ◽

Disease Risk ◽

Seed Extract ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Irvingia Gabonensis

Cardiotoxicity as an off-target effect of doxorubicin therapy is a major limiting factor for its clinical use as a choice cytotoxic agent. Seeds of Irvingia gabonensis have been reported to possess both nutritional and medicinal values which include antidiabetic, weight losing, antihyperlipidemic, and antioxidative effects. Protective effects of Irvingia gabonensis ethanol seed extract (IGESE) was investigated in doxorubicin (DOX)-mediated cardiotoxicity induced with single intraperitoneal injection of 15 mg/kg of DOX following the oral pretreatments of Wistar rats with 100-400 mg/kg/day of IGESE for 10 days, using serum cardiac enzyme markers (cardiac troponin I (cTI) and lactate dehydrogenase (LDH)), cardiac tissue oxidative stress markers (catalase (CAT), malonyldialdehyde (MDA), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), and reduced glutathione (GSH)), and cardiac histopathology endpoints. In addition, both qualitative and quantitative analyses to determine IGESE’s secondary metabolites profile and its in vitro antioxidant activities were also conducted. Results revealed that serum cTnI and LDH were significantly elevated by the DOX treatment. Similarly, activities of tissue SOD, CAT, GST, and GSH levels were profoundly reduced, while GPx activity and MDA levels were profoundly increased by DOX treatment. These biochemical changes were associated with microthrombi formation in the DOX-treated cardiac tissues on histological examination. However, oral pretreatments with 100-400 mg/kg/day of IGESE dissolved in 5% DMSO in distilled water significantly attenuated increases in the serum cTnI and LDH, prevented significant alterations in the serum lipid profile and the tissue activities and levels of oxidative stress markers while improving cardiovascular disease risk indices and DOX-induced histopathological lesions. The in vitro antioxidant studies showed IGESE to have good antioxidant profile and contained 56 major secondary metabolites prominent among which are γ-sitosterol, Phytol, neophytadiene, stigmasterol, vitamin E, hexadecanoic acid and its ethyl ester, Phytyl palmitate, campesterol, lupeol, and squalene. Overall, both the in vitro and in vivo findings indicate that IGESE may be a promising prophylactic cardioprotective agent against DOX-induced cardiotoxicity, at least in part mediated via IGESE’s antioxidant and free radical scavenging and antithrombotic mechanisms.

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