Proteome Analysis of Human Pancreatic Ductal Adenocarcinoma Tissue Using Two-Dimensional Gel Electrophoresis and Tandem Mass Spectrometry for Identification of Disease-Related Proteins

2007 ◽  
Vol 53 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Rui Tian ◽  
Li-Ming Wei ◽  
Ren-Yi Qin ◽  
Yan Li ◽  
Zhi-Yong Du ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Ductal Adenocarcinoma ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Related Proteins

Characterization of wheat gliadin proteins by combined two-dimensional gel electrophoresis and tandem mass spectrometry

PROTEOMICS ◽  
2005 ◽  
Vol 5 (11) ◽  
pp. 2859-2865 ◽  
Author(s):  
Gianfranco Mamone ◽  
Francesco Addeo ◽  
Lina Chianese ◽  
Aldo Di Luccia ◽  
Alessandra De Martino ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Wheat Gliadin

Proteomic profiling of sea bass muscle by two-dimensional gel electrophoresis and tandem mass spectrometry

2013 ◽  
Vol 40 (1) ◽  
pp. 311-322 ◽  
Author(s):  
Genciana Terova ◽  
Salvatore Pisanu ◽  
Tonina Roggio ◽  
Elena Preziosa ◽  
Marco Saroglia ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Gel Electrophoresis ◽  
Sea Bass ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Proteomic Profiling ◽  
Two Dimensional Gel Electrophoresis

Proteome Analysis of CD4+ T Cells Reveals Differentially Expressed Proteins in Infertile Polycystic Ovary Syndrome Patients

2020 ◽  
Vol 20 ◽  
Author(s):  
Fatemeh Nasri ◽  
Maryam Zare ◽  
Mehrnoosh Doroudchi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
Mass Spectrometry ◽  
T Cells ◽  
T Lymphocytes ◽  
Gel Electrophoresis ◽  
Cd4 T Cells ◽  
Polycystic Ovary ◽  
Proteome Analysis ◽  
Two Dimensional ◽  
Ovary Syndrome ◽  
Two Dimensional Gel Electrophoresis

Background: Polycystic ovary syndrome (PCOS) is the most frequent endocrine disorder affecting 6–7% of premenopausal women. Recent studies revealed that the immune system especially CD4+ T helper cells are important in the context PCOS. Proteome analysis of CD4+ T lymphocytes can provide valuable information regarding the biology of these cells in the context of PCOS. Objective: To investigate immune dysregulation in CD4+ T lymphocytes at the protein level in the context of PCOS using two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). Methods: In the present study, we applied two-dimensional gel electrophoresis / mass spectrometry to identify proteins differentially expressed by peripheral blood CD4+ T cells in ten PCOS women compared with ten healthy women. Western blot technique was used to confirm the identified proteins. Results: Despite the overall proteome similarities, there were significant differences in the expression of seven spots between two groups (P <0.05). Three proteins, namely phosphatidylethanolamine-binding protein 1, proteasome activator complex subunit 1 and triosephosphate isomerase 1 were successfully identified by Mass technique and confirmed by western blot. All characterized proteins were over-expressed in CD4+ T cells from patients compared to CD4+ T cells from controls (P <0.05). In-silico analysis suggested that the over-expressed proteins interact with other proteins involved in cellular metabolism especially glycolysis and ferroptosis pathway. Conclusion: These findings suggest that metabolic adjustments in CD4+ T lymphocytes, which is in favor of increased glycolysis and Th2 differentiation are important in the context of PCOS.

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