Silver nanoparticles inactivate sclerotial formation in controlling white rot disease in onion and garlic caused by the soil borne fungus Stromatinia cepivora

Author(s):  
Osama M. Darwesh ◽  
Ibrahim E. Elshahawy
2018 ◽  
Vol 100 (3) ◽  
pp. 493-503 ◽  
Author(s):  
Ibrahim E. Elshahawy ◽  
Nehal M. Saied ◽  
Farid Abd-El-Kareem ◽  
Ahmed A. Morsy

2009 ◽  
Vol 9 (2) ◽  
pp. 149-157
Author(s):  
Joko Prasetyo ◽  
Titik Nur Aeny ◽  
Radix Suharjo

The corelations between White Rot (Rigidoporus lignosus L.) incidence and  soil characters of rubber ecosystem in Penumangan Baru, Lampung. A study was conducted to evaluate the corelation between soil characters and white rot incidence. The study was done in Penumangan Lampung from June 2007 to July 2008. Seven sites were observed on the incidence of white rot of indicator plants (cassava). Physical, chemical, and biological soil characters were correlated and regressed with white rot incidence. The results showed that there were correlation between white rot incidence and soil characters such as pH KCl, number of fungal genus (fungal diversity), Ca+, total cation, CEC, and saturation base. Ion Ca+, total cation, saturation base correlated positively with white rot incidence, on the contrary, fungal diversity, cation exchange capacity, and pH KCl correlated negatively with white rot incidence. Thus, efforts to increase fungal diversity, cation exchange capacity, and pH (in acidic soil) could be used as basic strategies to develope intergrated  control of white rot disease.


AgriPeat ◽  
2019 ◽  
Vol 20 (02) ◽  
pp. 92-98
Author(s):  
Admin Journal

ABSTRACTThis study aims to determine effective management patterns to control Sclerotium rot and to improvepeat soil fertility with the application of the Trichoderma sp + Aspergillus sp consortium.as biologicalagents and P solvents, combined with spacing arrangements in the cultivation of scallion in peatlands.The study used a factorial randomized block design consisting of two factors with four replications.Factor I Application of biological agents consists of: A0 = No biological agents; A1 = Consortium ofTrichoderma sp. + Aspergillus sp; Factor II Plant spacing, consisting of J1 = 20x25 cm; J2 = 25x25cm and J3 = 30x25 cm. The results showed that the interaction treatment of Trichoderma sp. +Aspergillus sp. and spacing of 25x25 cm effectively suppresses the incidence of white rot disease(Sclerotium cepivorum Berk) up to 34.02%, while at a spacing of 20x25 cm with the application ofTrichoderma sp. + Aspergillus sp. produced the highest fresh plant weight of 8.80 kg plot-1 or 24.44tons hectares-1. Increasing the number of leaves is only influenced by a single factor of biologicalagents (23.29%) and spacing of 25x25 cm (19.7%). Application of Trichoderma sp. + Aspergillus sp.can increase the nutrient content of N, P (total and available), K and peat soil organic matter.Consortium of biological agents Trichoderma sp. and Aspergillus sp. indigenous have the potential tobe developed as biological agents and biofertilizers, with optimum spacing can be applied to themanagement of scallion cultivation in peatlands.Key words: Sclerotium cepivorum Berk, scallion, Trichoderma sp. and Aspergillus sp., spacing


Forests ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 885 ◽  
Author(s):  
Casado-Sanz ◽  
Silva-Castro ◽  
Ponce-Herrero ◽  
Martín-Ramos ◽  
Martín-Gil ◽  
...  

There is growing interest in the development of non-toxic, natural wood preservation agents to replace conventional chemicals. In this paper, the antifungal activities of silver nanoparticles, chitosan oligomers, and propolis ethanolic extract were evaluated against white-rot fungus Trametes versicolor (L.) Lloyd, with a view to protecting Populus spp. wood. In order to create a more realistic in-service type environment, the biocidal products were assessed according to EN:113 European standard, instead of using routine in vitro antimicrobial susceptibility testing methods. Wood blocks were impregnated with the aforementioned antifungal agents by the vacuum-pressure method in an autoclave, and their biodeterioration was monitored over 16 weeks. The results showed that treatments based on silver nanoparticles, at concentrations ranging from 5 to 20 ppm, presented high antifungal activity, protecting the wood from fungal attack over time, with weight losses in the range of 8.49% to 8.94% after 16 weeks, versus 24.79% weight loss in the control (untreated) samples. This was confirmed by SEM and optical microscopy images, which showed a noticeably higher cell wall degradation in control samples than in samples treated with silver nanoparticles. On the other hand, the efficacy of the treatments based on chitosan oligomers and propolis gradually decreased over time, which would be a limiting factor for their application as wood preservatives. The nanometal-based approach is thus posed as the preferred choice for the industrial treatment of poplar wood aimed at wood-based engineering products (plywood, laminated veneer lumber, cross-laminated timber, etc.).


2016 ◽  
Vol 96 (2) ◽  
pp. 243-251 ◽  
Author(s):  
Dorota Remlein-Starosta ◽  
Joanna Krzymińska ◽  
Jolanta Kowalska ◽  
Jan Bocianowski

Virginia mallow is a plant from the central and eastern states of North America. A large amount of high quality biomass obtained from V. mallow led to interest in this plant for bioenergy purposes. Unfortunately, high incidence of white rot disease caused by Sclerotinia sclerotiorum destroyed plantations of this plant. In previous attempts, various control strategies were not successful and all treatments failed (including the use of Coniothurium minitans). The aim of the study was to find and evaluate yeast-like fungi for biocontrol of S. sclerotiorum, evaluate the optimal thermal condition for biocontrol efficacy, and describe possible modes of action of potential biocontrol organisms. In dual bioassay test with 19 strains of yeast-like fungi with S. sclerotiorum, antagonistic potential were obtained. At different temperatures variations in antagonistic activity of yeast-like fungi were observed. At all tested temperatures (i.e., 4, 12, and 23°C), positive results were obtained. Extracellular enzymes were produced by the majority of antagonistic yeast, such as: amylases (the most frequent), chitinases, proteases, pectinases and xylanases. In this research, reduction in white rot symptoms on V. mallow stems was significant (P < 0.05). The highest inhibition of disease was observed after treatment with strains (114/64) Candida albidus and (117/10) Pichia anomala.


Author(s):  
Marwa A. Samy ◽  
Moustafa A. Abbassy ◽  
Elsayed E. Hafez ◽  
Entsar I. Rabea ◽  
Dalia G. Aseel

Solanum tubersum is the fourth most challenging plant in Egypt, affected by several fungi, viral and bacterial diseases. Bacterial and fungal isolates (Brown rot disease (Ralstonia solaniserum), soft root disease (Pectobacterium carotovora) and dry rot disease (Fusarium oxisporum) were collected. The green extracts of silver nanoparticles were prepared by means of aqueous extracts of three wild plants, Physalis peruviana (leaves, red and green fruits) (N1, N2 and N3), Solanum nigrum (fruit) (N4) and Moringa oliefera (leaves) (N5). SEM, TEM, FT-IR and X-RD obtained the characterization of the biosynthesis of silver nanoparticles. The results indicated that nanoparticles were spherical, smooth and the sizes varied between 12 and 33 nm. The activity of the nanoparticle formulations was tested against the bacterial isolates using agar diffusion method and one fungus using mycelial growth method. The results also elucidated that N5 formulation showed a significantly potent antibacterial activity against R. solanacearum. However, N1 formulation was the highest active one against P. carotovra. In addition, the antifungal activity indicated that N1 had the highest effect (EC50 = 687.03 mg/L) followed by N3 (EC50 = 981.61 mg/L) against F. oxysporium. Nanoparticles synthesized by wild plants could be used as safe alternatives to harmful microbicides.


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