Genetic characterization and gene flow in different geographical-distance neighbouring natural populations of wild soybean (Glycine soja Sieb. & Zucc.) and implications for protection from GM soybeans

Abstract Gene flow from transgenic crops to conventional cultivars or wild relatives is a major environmental and economic concern in many countries. South Korea is one of the major importer of transgenic crops for food and feed, although commercial cultivation of transgenic crops is not yet allowed in this country. This study evaluated gene flow from the herbicide glyphosate- and glufosinate-resistant transgenic soybean (Glycine max) to five non-transgenic soybean cultivars and three accessions of wild soybean (Glycine soja). Field trials were conducted over 2 years, and gene flow was monitored up to 10 m distance from the pollen source. The results indicated that the detectable rate of gene flow from transgenic to conventional soybeans varied between 0 and 0.049% in both 2014 and 2015 field trials, while no hybrids were detected among wild soybean progenies. The highest rate of gene flow was found in the progenies of the Bert cultivar, which exhibited the longest period of flowering synchronization between the pollen donor and the recipient. In addition, overall gene flow rates declined with increased distance from the transgenic soybean plot. Gene flow was observed up to 3 m and 8 m from the transgenic soybean plot in 2014 and 2015, respectively. Our results may be useful for developing measures to prevent gene flow from transgenic soybean.

Download Full-text

Likelihood assessment for gene flow of transgenes from imported genetically modified soybean (Glycine max (L.) Merr.) to wild soybean (Glycine soja Seib. et Zucc.) in Japan as a component of environmental risk assessment

Breeding Science ◽

10.1270/jsbbs.16134 ◽

2017 ◽

Vol 67 (4) ◽

pp. 348-356 ◽

Cited By ~ 4

Author(s):

Hidetoshi Goto ◽

Marc A. McPherson ◽

Bradley A. Comstock ◽

Duška Stojšin ◽

Ryo Ohsawa

Keyword(s):

Risk Assessment ◽

Glycine Max ◽

Gene Flow ◽

Environmental Risk ◽

Environmental Risk Assessment ◽

Glycine Soja ◽

Genetically Modified ◽

Wild Soybean ◽

Genetically Modified Soybean

Download Full-text

Genetic diversity and gene flow dynamics revealed in the rare mixed populations of wild soybean (Glycine soja) and semi-wild type (Glycine gracilis) in China

Genetic Resources and Crop Evolution ◽

10.1007/s10722-013-9998-8 ◽

2013 ◽

Vol 60 (8) ◽

pp. 2303-2318 ◽

Cited By ~ 3

Author(s):

Ke-Jing Wang ◽

Xiang-Hua Li

Keyword(s):

Genetic Diversity ◽

Gene Flow ◽

Glycine Soja ◽

Wild Soybean ◽

Flow Dynamics ◽

Wild Type ◽

Mixed Populations

Download Full-text

A Novel Pinkish-White Flower Color Variant Is Caused by a New Allele of Flower Color Gene W1 in Wild Soybean (Glycine soja)

Agronomy ◽

10.3390/agronomy11051001 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1001

Author(s):

Jagadeesh Sundaramoorthy ◽

Gyu Tae Park ◽

Hyun Jo ◽

Jeong-Dong Lee ◽

Hak Soo Seo ◽

...

Keyword(s):

Amino Acid ◽

Amino Acid Substitution ◽

Functional Activity ◽

Glycine Soja ◽

Wild Soybean ◽

Flower Color ◽

Loss Of Function ◽

Protein Variation ◽

Color Variant ◽

Color Gene

The enzyme flavonoid 3′,5′-hydroxylase (F3′5′H) plays an important role in producing anthocyanin pigments in soybean. Loss of function of the W1 locus encoding F3′5′H always produces white flowers. However, few color variations have been reported in wild soybean. In the present study, we isolated a new color variant of wild soybean accession (IT261811) with pinkish-white flowers. We found that the flower’s pinkish-white color is caused by w1-s3, a single recessive allele of W1. The SNP detected in the mutant caused amino acid substitution (A304S) in a highly conserved SRS4 domain of F3′5′H proteins. On the basis of the results of the protein variation effect analyzer (PROVEAN) tool, we suggest that this mutation may lead to hypofunctional F3′5′H activity rather than non-functional activity, which thereby results in its pinkish-white color.

Download Full-text

Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

Plant Genetic Resources ◽

10.1017/s1479262121000071 ◽

2021 ◽

Vol 19 (1) ◽

pp. 35-43

Author(s):

Awatsaya Chotekajorn ◽

Takuyu Hashiguchi ◽

Masatsugu Hashiguchi ◽

Hidenori Tanaka ◽

Ryo Akashi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Free Amino Acid ◽

Acid Content ◽

Free Amino ◽

Glycine Soja ◽

Wild Soybean ◽

Amino Acid Content ◽

Soybean Seeds ◽

Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

Download Full-text