scholarly journals Dose-response effects of Asparagopsis taxiformis and Oedogonium sp. on in vitro fermentation and methane production

2015 ◽  
Vol 28 (2) ◽  
pp. 1443-1452 ◽  
Author(s):  
Lorenna Machado ◽  
Marie Magnusson ◽  
Nicholas A. Paul ◽  
Robert Kinley ◽  
Rocky de Nys ◽  
...  
Keyword(s):  
Dose Response ◽  
Methane Production ◽  
In Vitro Fermentation ◽  
Asparagopsis Taxiformis

The red macroalgae Asparagopsis taxiformis is a potent natural antimethanogenic that reduces methane production during in vitro fermentation with rumen fluid

2016 ◽  
Vol 56 (3) ◽  
pp. 282 ◽  
Author(s):  
Robert D. Kinley ◽  
Rocky de Nys ◽  
Matthew J. Vucko ◽  
Lorenna Machado ◽  
Nigel W. Tomkins
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Negative Impact ◽  
Rumen Fluid ◽  
In Vitro Fermentation ◽  
Red Macroalgae ◽  
Asparagopsis Taxiformis ◽  
Enteric Methane ◽  
Dose Levels

Livestock feed modification is a viable method for reducing methane emissions from ruminant livestock. Ruminant enteric methane is responsible approximately to 10% of greenhouse gas emissions in Australia. Some species of macroalgae have antimethanogenic activity on in vitro fermentation. This study used in vitro fermentation with rumen inoculum to characterise increasing inclusion rates of the red macroalga Asparagopsis taxiformis on enteric methane production and digestive efficiency throughout 72-h fermentations. At dose levels ≤1% of substrate organic matter there was minimal effect on gas and methane production. However, inclusion ≥2% reduced gas and eliminated methane production in the fermentations indicating a minimum inhibitory dose level. There was no negative impact on substrate digestibility for macroalgae inclusion ≤5%, however, a significant reduction was observed with 10% inclusion. Total volatile fatty acids were not significantly affected with 2% inclusion and the acetate levels were reduced in favour of increased propionate and, to a lesser extent, butyrate which increased linearly with increasing dose levels. A barrier to commercialisation of Asparagopsis is the mass production of this specific macroalgal biomass at a scale to provide supplementation to livestock. Another area requiring characterisation is the most appropriate method for processing (dehydration) and feeding to livestock in systems with variable feed quality and content. The in vitro assessment method used here clearly demonstrated that Asparagopsis can inhibit methanogenesis at very low inclusion levels whereas the effect in vivo has yet to be confirmed.

332 Effect of Chemical and Biological Preservatives on in vitro Fermentation and Methane Production of Ensiled and Aerobically Exposed Wet Brewer’s Grains

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 182-182
Author(s):  
Marjorie A Killerby ◽  
Diego Zamudio ◽  
Kaycee Ames ◽  
Darren D Henry ◽  
Thomas Schwartz ◽  
...  
Keyword(s):  
Methane Production ◽  
Production System ◽  
Methane Concentration ◽  
Block Design ◽  
Gas Production ◽  
Sodium Lignosulfonate ◽  
In Vitro Fermentation ◽  
Randomized Complete Block Design ◽  
System Data

Abstract This study evaluated the effects of preservatives on the in vitro fermentation measures of wet brewer’s grain (WBG) silage at different stages of storage. Treatments (TRT) were sodium lignosulfonate at 1% (NaL1) and 2% (NaL2; w/w of fresh WBG), propionic acid (PRP; 0.5% w/w of fresh WBG), a combination inoculant (INO; Lactococcus lactis and Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated WBG (CON). WBG (Fresh) were packed into 8.8 L mini-silos and stored for 60 d at 21°C (Ensiled), then they were opened and aerobically exposed for 10d (AES). Samples from each stage of storage (STG; Fresh, Ensiled and AES) were analyzed for in vitro ruminal digestibility (24 h).Gas kinetics were recorded using the Ankom RF Gas Production System. Data were analyzed as a randomized complete block design (5 blocks) with a 5 (TRT) × 3 (STG) factorial arrangement. Apparent in vitro DM digestibility (DMD) decreased across STG, (51.5, 47.2 and 40.9 for Fresh, Ensiled and AES, respectively) and increased for NaL1, NaL2 and PRP (~47.8) vs. CON (43.0 ± 2.12%). PRP increased apparent in vitro OM digestibility (OMD) when Ensiled (54.5) and NaL2 increased it for AES (47.1) vs CON (46.3 and 39.9 ± 1.73%, respectively). The asymptotic maximal (M) and rate (k) of gas production decreased across STG (214.6, 181.5, 155.1 and 14.6, 12.6, and 9.8, for Fresh, Ensiled and AES, respectively). PRP increased (200.0) and NaL1 decreased (169.3) M vs. CON (183.9± 7.81ml/incubated DM g), while NaL1 and NaL2 (~11.4) decreased k vs. CON (13.4 ± 0.85%/h). Methane concentration and yield were higher in Fresh vs. other STG (0.94 vs. ~0.84 ± 0.07mM and 0.27 vs. ~0.23 ± 0.03mmol/g fermented OM). Spoilage of WBG decreases fermentability and methane production while PRP and NaL improve digestibility with the former also increasing M and k.

scholarly journals Modelling the impact of the macroalgae Asparagopsis taxiformis on rumen microbial fermentation

2020 ◽  
Author(s):  
Rafael Muñoz-Tamayo ◽  
Juana C. Chagas ◽  
Mohammad Ramin ◽  
Sophie J. Krizsan
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Mean Squared Error ◽  
Microbial Fermentation ◽  
Model Structure ◽  
Red Macroalgae ◽  
Asparagopsis Taxiformis ◽  
The Impact

AbstractBackgroundThe red macroalgae Asparagopsis taxiformis is a potent natural supplement for reducing methane production from cattle. A. taxiformis contains several anti-methanogenic compounds including bromoform that inhibits directly methanogenesis. The positive and adverse effects of A. taxiformis on the rumen microbiota are dose-dependent and operate in a dynamic fashion. It is therefore key to characterize the dynamic response of the rumen microbial fermentation for identifying optimal conditions on the use of A. taxiformis as a dietary supplement for methane mitigation. Accordingly, the objective of this work was to model the effect of A. taxiformis supplementation on the rumen microbial fermentation under in vitro conditions. We adapted a published mathematical model of rumen microbial fermentation to account for A. taxiformis supplementation. We modelled the impact of A. taxiformis on the fermentation and methane production by two mechanisms, namely (i) direct inhibition of the growth rate of methanogenesis by bromoform and (ii) hydrogen control on sugars utilization and on the flux distribution towards volatile fatty acids production. We calibrated our model using a multi-experiment estimation approach that integrated experimental data with six macroalgae supplementation levels from a published in vitro study assessing the dose-response impact of A. taxiformis on rumen fermentation.Resultsour model captured satisfactorily the effect of A. taxiformis on the dynamic profile of rumen microbial fermentation for the six supplementation levels of A. taxiformis with an average determination coefficient of 0.88 and an average coefficient of variation of the root mean squared error of 15.2% for acetate, butyrate, propionate, ammonia and methane.Conclusionsour results indicated the potential of our model as prediction tool for assessing the impact of additives such as seaweeds on the rumen microbial fermentation and methane production in vitro. Additional dynamic data on hydrogen and bromoform are required to validate our model structure and look for model structure improvements. We are working on model extensions to account for in vivo conditions. We expect this model development can be useful to help the design of sustainable nutritional strategies promoting healthy rumen function and low environmental footprint.

Effect of monensin on in vitro fermentation of maize starch by hindgut contents of cattle

1990 ◽  
Vol 115 (3) ◽  
pp. 389-392 ◽  
Author(s):  
M. Marounek ◽  
O. Petr ◽  
L. Machañová
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Maize Starch ◽  
Total Volatile ◽  
Molar Percentage ◽  
In Vitro Fermentation ◽  
Consistent Effect ◽  
Total Volatile Fatty Acid

SUMMARYContents of the caecum and the colon of four steers and four cows fed concentrate-plus-roughage diets were obtained at an abattoir, transferred to the laboratory and incubated anaerobically with maize starch in the presence (10mg/l) or absence of monensin. Samples taken at the end of incubation were assayed for fermentation acids and methane production.Monensin significantly increased the molar percentage of propionate and decreased that of butyrate. Acetate percentage was not significantly influence by the addition of monensin. There was no consistent effect of monensin on total volatile fatty acid concentrations. Monensin decreased methanogenesis in all incubations. The production of methane was small, only 1 mmol/13–50 mmol of volatile fatty acid.

scholarly journals Effects of replacing barley grain in feedlot diets with increasing levels of glycerol on in vitro fermentation and methane production

2011 ◽  
Vol 166-167 ◽  
pp. 265-268 ◽  
Author(s):  
J.S. Avila ◽  
A.V. Chaves ◽  
M. Hernandez-Calva ◽  
K.A. Beauchemin ◽  
S.M. McGinn ◽  
...  
Keyword(s):  
Methane Production ◽  
Barley Grain ◽  
In Vitro Fermentation

Dose–response effects of essential oils on in vitro fermentation activity of the rumen microbial population

2008 ◽  
Vol 145 (1-4) ◽  
pp. 335-350 ◽  
Author(s):  
D. Macheboeuf ◽  
D.P. Morgavi ◽  
Y. Papon ◽  
J.-L. Mousset ◽  
M. Arturo-Schaan
Keyword(s):  
Essential Oils ◽  
Dose Response ◽  
Microbial Population ◽  
In Vitro Fermentation

scholarly journals The Inclusion of Concentrate with Quebracho Is Advisable in Two Forage-Based Diets of Ewes According to the In Vitro Fermentation Parameters

Animals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 451 ◽  
Author(s):  
Pablo Jose Rufino-Moya ◽  
Mireia Blanco ◽  
Sandra Lobón ◽  
Juan Ramon Bertolín ◽  
Ramón Armengol ◽  
...  
Keyword(s):  
Methane Production ◽  
Valeric Acid ◽  
Ruminal Fermentation ◽  
In Vitro Fermentation ◽  
N Content ◽  
Ch4 Production ◽  
Branched Chain ◽  
Fermentation Parameters ◽  
Forage Diets

Ewes receive hay or graze on fresh pastures supplemented with concentrates to fulfil their lactation requirements. Quebracho (Schinopsis balansae) can be added to change the ruminal fermentation. Fermentation parameters of forages alone and 70:30 forage:concentrate diets with control and quebracho concentrate were compared after 24 h of in vitro incubation. Fresh forage diets produced less gas (p < 0.05) and had greater IVOMD (p < 0.001), ammonia (NH3-N) content, valeric acid, branched-chain volatile fatty acid proportions, and lower propionic acid proportion than the hay diets (p < 0.01). In the hay diets, methane production increased with control concentrate (p < 0.01) and tended to decrease with quebacho concentrate (p < 0.10). The inclusion of both concentrates increased the acetic:propionic ratio (p < 0.01), and only the inclusion of quebracho concentrate increased the IVOMD (p < 0.01). In the fresh forage diets, gas and methane production increased with the inclusion of the control concentrate (p < 0.05), but methane production decreased with quebracho concentrate (p < 0.01). The inclusion of quebracho concentrate reduced the NH3-N content and valeric acid proportion (p < 0.05). In conclusion, the inclusion of quebracho concentrate would be advisable to reduce the CH4 production and NH3-N content in fresh forage diets and to increase the IVOMD in hay diets in comparison with the forages alone.

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