scholarly journals Quantitative label-free proteomics and biochemical analysis of Phaeodactylum tricornutum cultivation on dairy manure wastewater

2021 ◽  
Author(s):  
Andrew R. Burch ◽  
Cody W. Yothers ◽  
Michelle R. Salemi ◽  
Brett S. Phinney ◽  
Pramod Pandey ◽  
...  
Keyword(s):  
Protein Trafficking ◽  
Phaeodactylum Tricornutum ◽  
Biochemical Analysis ◽  
Docosapentaenoic Acid ◽  
Dairy Manure ◽  
Label Free ◽  
Wastewater Remediation ◽  
Feedstock Production ◽  
Synthetic Media ◽  
And Oxidative Stress

AbstractMicroalgae cultivation on wastewater offers the dual benefit of lowering costs for feedstock production with simultaneous wastewater remediation. This study utilized biochemical and quantitative label-free proteomic approaches to evaluate the growth and proteomic response for diatom Phaeodactylum tricornutum cultivated on flushed dairy manure wastewater (DMW). Comparing several DMW dilutions (up to 60% DMW diluted in seawater) with a synthetic seawater medium indicates that biomass and lipid yields correlate with the starting nitrogen content of the DMW dilution. Phaeodactylum tricornutum cultivated on DMW exhibits elevated levels of polyunsaturated fatty acids (PUFAs), particularly docosapentaenoic acid (DPA, 22:5 n-3). Proteomic analysis revealed alterations in the regulations of proteins associated with protein metabolism, cellular signaling, transcription and translation, protein trafficking, and oxidative stress management pathways when comparing P. tricornutum cultivation on diluted DMW versus synthetic media, thus providing insights into how P. tricornutum reorganizes its proteome in response to a complex wastewater source. Graphical abstract

scholarly journals Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae Phaeodactylum tricornutum Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 119 ◽  
Author(s):  
Alok Patel ◽  
Leonidas Matsakas ◽  
Kateřina Hrůzová ◽  
Ulrika Rova ◽  
Paul Christakopoulos
Keyword(s):  
Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Fish Oil ◽  
Phaeodactylum Tricornutum ◽  
Forest Biomass ◽  
Docosapentaenoic Acid ◽  
Raw Material ◽  
Human Function ◽  
Dha Production ◽  
Synthetic Media

Polyunsaturated fatty acids (PUFAs) are essential for human function, however they have to be provided through the diet. As their production from fish oil is environmentally unsustainable, there is demand for new sources of PUFAs. The aim of the present work was to establish the microalgal platform to produce nutraceutical-value PUFAs from forest biomass. To this end, the growth of Phaeodactylum tricornutum on birch and spruce hydrolysates was compared to autotrophic cultivation and glucose synthetic media. Total lipid generated by P. tricornutum grown mixotrophically on glucose, birch, and spruce hydrolysates was 1.21, 1.26, and 1.29 g/L, respectively. The highest eicosapentaenoic acid (EPA) production (256 mg/L) and productivity (19.69 mg/L/d) were observed on spruce hydrolysates. These values were considerably higher than those obtained from the cultivation without glucose (79.80 mg/L and 6.14 mg/L/d, respectively) and also from the photoautotrophic cultivation (26.86 mg/L and 2.44 mg/L/d, respectively). To the best of our knowledge, this is the first report describing the use of forest biomass as raw material for EPA and docosapentaenoic acid (DHA) production.

Differential susceptibility of a few members of the nasuta–albomicans complex of Drosophila to paraquat-induced lethality and oxidative stress

Genome ◽  
2011 ◽  
Vol 54 (10) ◽  
pp. 829-835 ◽  
Author(s):  
Mysore S. Ranjini ◽  
Ravikumar Hosamani ◽  
Muralidhara ◽  
Nallur B. Ramachandra
Keyword(s):  
Oxidative Stress ◽  
Biochemical Analysis ◽  
Reduced Glutathione ◽  
Differential Susceptibility ◽  
Activity Levels ◽  
Oxygen Species ◽  
Stress Markers ◽  
The Status ◽  
Markers Of Oxidative Stress ◽  
And Oxidative Stress

The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cytoraces, employing the well known paraquat (PQ) resistance bioassay. Exposure of these races to varying concentrations of PQ revealed relatively higher resistance among LL cytoraces than SL cytoraces, as evident by the lower incidence of mortality. Biochemical analysis for endogenous markers of oxidative stress revealed that LL-2 cytorace exhibited lower reactive oxygen species (ROS) and lipid peroxidation (LPO) levels, higher activity levels of superoxide dismutase (SOD), and coupled with higher levels of reduced glutathione (GSH) compared with the levels found in SL-2 cytorace. These findings suggest that the higher susceptibility of SL cytoraces to PQ challenge may be, at least in part, related to the higher endogenous levels of oxidative stress markers. Although the precise mechanisms responsible for the longer longevity among LL cytoraces of the nasuta–albomicans complex of Drosophila merits further investigation, our data suggest that the relatively longer lifespan may be related to the status of endogenous markers that renders them more resistant towards oxidative-stress-mediated lethality, as evident in the PQ assay.

Dairy Manure Wastewater Remediation Using Non-airtight Digestion Pretreatment Followed by Microalgae Cultivation

2020 ◽  
Vol 192 (3) ◽  
pp. 1093-1105
Author(s):  
Liang Wang ◽  
Lide Chen ◽  
Wu Sarah ◽  
Muhammad Aamir Bashir
Keyword(s):  
Dairy Manure ◽  
Wastewater Remediation ◽  
Microalgae Cultivation

scholarly journals Label-Free Proteomics Revealed Oxidative Stress and Inflammation as Factors That Enhance Chemoresistance in Luminal Breast Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Bruno R. B. Pires ◽  
Carolina Panis ◽  
Vinícius Dias Alves ◽  
Ana C. S. A. Herrera ◽  
Renata Binato ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Luminal Breast Cancer ◽  
Label Free ◽  
Luminal A ◽  
Protein Protein Interaction ◽  
Protein Protein Interaction Networks ◽  
Cancer Chemoresistance ◽  
The Complement System ◽  
And Oxidative Stress

Breast cancer is the leading cause of cancer-associated death among women worldwide. Its high mortality rate is related to resistance towards chemotherapies, which is one of the major challenges of breast cancer research. In this study, we used label-free mass spectrometry- (MS-) based proteomics to investigate the differences between circulating proteins in the plasma of patients with chemoresponsive and chemoresistant luminal A breast cancer. MS analysis revealed 205 differentially expressed proteins. Furthermore, we used in silico tools to build protein-protein interaction networks. Most of the upregulated proteins in the chemoresistant group were closely related and tightly linked. The predominant networks were related to oxidative stress, the inflammatory response, and the complement cascade. Through this analysis, we identified inflammation and oxidative stress as central processes of breast cancer chemoresistance. Furthermore, we confirmed our hypothesis by evaluating oxidative stress and performing cytokine profiling in our cohort. The connections among oxidative stress, inflammation, and the complement system described in our study seem to indicate a pivotal axis in breast cancer chemoresistance. Hence, these findings will have significant clinical implications for improving therapies to bypass breast cancer chemoresistance in the future.

Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea ( Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV)

2017 ◽  
Vol 163 ◽  
pp. 76-91 ◽  
Author(s):  
Anna Lidia N. Varela ◽  
Setsuko Komatsu ◽  
Xin Wang ◽  
Rodolpho G.G. Silva ◽  
Pedro Filho N. Souza ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Vigna Unguiculata ◽  
Biochemical Analysis ◽  
Label Free

scholarly journals Infrared spectroscopic imaging: Label-free biochemical analysis of stroma and tissue fibrosis

2017 ◽  
Vol 92 ◽  
pp. 14-17 ◽  
Author(s):  
Shaiju S. Nazeer ◽  
Hari Sreedhar ◽  
Vishal K. Varma ◽  
David Martinez-Marin ◽  
Christine Massie ◽  
...  
Keyword(s):  
Biochemical Analysis ◽  
Spectroscopic Imaging ◽  
Label Free ◽  
Tissue Fibrosis ◽  
Infrared Spectroscopic ◽  
Infrared Spectroscopic Imaging

scholarly journals Intricate effects of primary motor neuronopathy on contractile proteins and metabolic muscle enzymes as revealed by label-free mass spectrometry

2014 ◽  
Vol 34 (4) ◽  
Author(s):  
Ashling Holland ◽  
Thomas Schmitt-John ◽  
Paul Dowling ◽  
Paula Meleady ◽  
Michael Henry ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Energy Metabolism ◽  
Mouse Model ◽  
Motor Neuron ◽  
Biochemical Analysis ◽  
Protein Profile ◽  
Label Free ◽  
Contractile Apparatus ◽  
Muscle Enzymes ◽  
Neuromuscular Synapses

The systematic biochemical analysis of muscle from the wobbler mouse model of motor neuron disease suggests that the loss of neuromuscular synapses causes complex changes in the protein profile of contractile tissues, affecting especially the contractile apparatus and energy metabolism.

Label-free non-destructive in situ biochemical analysis of nematode Steinernema kraussei using FPA-FTIR and Raman spectroscopic imaging

2012 ◽  
Vol 60 ◽  
pp. 34-42 ◽  
Author(s):  
Katherine Lau ◽  
Alison Hobro ◽  
Tim Smith ◽  
Thomas Thurston ◽  
Bernard Lendl
Keyword(s):  
Biochemical Analysis ◽  
Spectroscopic Imaging ◽  
Label Free ◽  
Raman Spectroscopic ◽  
Non Destructive
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