Early cleaving embryos result in blastocysts with increased aspartate and glucose consumption, which exhibit different metabolic gene expression that persists in placental and fetal tissues

Skeletal muscle possesses a high degree of plasticity and can adapt to both the physical and metabolic challenges that it faces. An acute bout of exercise is sufficient to induce the expression of a variety of metabolic genes, such as GLUT4, pyruvate dehydrogenase kinase 4 (PDK-4), uncoupling protein-3 (UCP3), and peroxisome proliferator-activated receptor-γ coactivator 1 (PGC-1). Reducing muscle glycogen levels before exercise potentiates the effect of exercise on many genes. Similarly, altered substrate availability induces transcription of many of these genes. The purpose of this study was to determine whether glucose ingestion attenuates the exercise-induced increase in a variety of exercise-responsive genes. Six male subjects (28 ± 7 yr; 83 ± 3 kg; peak pulmonary oxygen uptake = 46 ± 6 ml·kg−1·min−1) performed 60 min of cycling at 74 ± 2% of peak pulmonary oxygen uptake on two separate occasions. On one occasion, subjects ingested a 6% carbohydrate drink. On the other occasion, subjects ingested an equal volume of a sweet placebo. Muscle samples were obtained from vastus lateralis at rest, immediately after exercise, and 3 h after exercise. PDK-4, UCP3, PGC-1, and GLUT4 mRNA levels were measured on these samples using real-time RT-PCR. Glucose ingestion attenuated ( P < 0.05) the exercise-induced increase in PDK-4 and UCP3 mRNA. A similar trend ( P = 0.09) was observed for GLUT4 mRNA. In contrast, PGC-1 mRNA increased following exercise to the same extent in both conditions. These data suggest that glucose availability can modulate the effect of exercise on metabolic gene expression.

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Cardiac Raptor Ablation Impairs Adaptive Hypertrophy, Alters Metabolic Gene Expression, and Causes Heart Failure in Mice

Circulation ◽

10.1161/circulationaha.110.977066 ◽

2011 ◽

Vol 123 (10) ◽

pp. 1073-1082 ◽

Cited By ~ 157

Author(s):

Pankaj Shende ◽

Isabelle Plaisance ◽

Christian Morandi ◽

Corinne Pellieux ◽

Corinne Berthonneche ◽

...

Keyword(s):

Gene Expression ◽

Heart Failure ◽

Metabolic Gene ◽

Metabolic Gene Expression

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Abstract 17010: Metabolic Gene Expression and Mitochondrial Function Are Altered in the Failing Single Ventricle Myocardium

Circulation ◽

10.1161/circ.138.suppl_1.17010 ◽

2018 ◽

Vol 138 (Suppl_1) ◽

Author(s):

Anastacia M Garcia ◽

Kathryn C Chatfield ◽

Genevieve C Sparagna ◽

Elisabeth K Phillips ◽

Anis Karimpour-Fard ◽

...

Keyword(s):

Gene Expression ◽

Mitochondrial Function ◽

Molecular Mechanisms ◽

Single Ventricle ◽

Normal Function ◽

P Value ◽

Current Standard ◽

Illumina Hiseq ◽

Metabolic Gene ◽

Metabolic Gene Expression

Introduction: Despite current standard of care, heart failure (HF) remains a leading cause of death and indication for transplant in the single ventricle congenital heart disease (SV) population. However, little is known regarding the molecular mechanisms underlying remodeling and eventual HF in SV patients. The purpose of this study was to characterize the transcriptional profile of SV myocardium in both failing (SVHF) and non-failing (SVNF) SV patients compared to biventricular NF controls (BVNF). Furthermore, we conducted high resolution respirometry to assess mitochondrial function in each of these populations. Methods: Library prep was performed using the TruSeq Ribo Zero rRNA depletion kit, and 2x150 total RNAseq (Illumina HiSEQ 4000) was performed on age-matched explanted RV myocardium from BVNF (n=4 biventricular donors), SVNF (n=8 SV primary transplants, normal function), and SVHF (n=9 SV systolic HF transplants). Samples were aligned to hg19 and were normalized and annotated using the edgeR pipeline. Significant changes in gene expression were calculated using an FDR adjusted p-value (q<0.1; p<0.025). Respiration of myocardial mitochondria was measured using a stepwise protocol to evaluate respiratory capacity in an Oroboros Oxygraph system; n=6 SVHF, n=6 SVNF, n=18 BVNF. Results: RNAseq identified 1,007 differentially expressed genes in SVNF and 2,109 in SVHF myocardium relative to BVNF controls. Transcriptome pathway analysis demonstrated multiple pathways that are similarly dysregulated in SVNF and SVHF, while pathways involved in mitochondrial metabolism and function were significantly dysregulated specifically in the SVHF population. Moreover, mitochondrial oxygen flux was significantly decreased, particularly through complexes I and II, in SVHF relative to BVNF controls. Conclusions: Our results provide new insights into SVHF by identifying unique gene expression changes, including those related to metabolism, and impaired mitochondrial function. Together these data suggest dysregulated metabolic gene expression and mitochondrial dysfunction are phenotypes associated with the failing single ventricle and may serve as potential therapeutic targets for the treatment or prevention of HF in the SV population.

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Green tea, black tea, and epigallocatechin modify body composition, improve glucose tolerance, and differentially alter metabolic gene expression in rats fed a high-fat diet

Nutrition Research ◽

10.1016/j.nutres.2009.10.003 ◽

2009 ◽

Vol 29 (11) ◽

pp. 784-793 ◽

Cited By ~ 119

Author(s):

Nora Chen ◽

Rebecca Bezzina ◽

Edward Hinch ◽

Paul A. Lewandowski ◽

David Cameron-Smith ◽

...

Keyword(s):

Gene Expression ◽

Body Composition ◽

Glucose Tolerance ◽

Green Tea ◽

High Fat Diet ◽

Black Tea ◽

High Fat ◽

Metabolic Gene ◽

Metabolic Gene Expression ◽

Improve Glucose Tolerance

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Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle

Journal of Applied Physiology ◽

10.1152/japplphysiol.01185.2004 ◽

2005 ◽

Vol 98 (5) ◽

pp. 1745-1752 ◽

Cited By ~ 175

Author(s):

Yifan Yang ◽

Andrew Creer ◽

Bozena Jemiolo ◽

Scott Trappe

Keyword(s):

Gene Expression ◽

Time Course ◽

Acute Exercise ◽

Vastus Lateralis ◽

Gene Induction ◽

Mrna Levels ◽

Metabolic Gene ◽

Expression Studies ◽

Metabolic Gene Expression ◽

Gene Expression Studies

The aim of this study was to examine the time course activation of select myogenic (MRF4, Myf5, MyoD, myogenin) and metabolic (CD36, CPT1, HKII, and PDK4) genes after an acute bout of resistance (RE) or run (Run) exercise. Six RE subjects [25 ± 4 yr (mean ± SD), 74 ± 14 kg, 1.71 ± 0.11 m] and six Run subjects (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m, 63 ± 8 ml·kg−1·min−1) were studied. Eight muscle biopsies were taken from the vastus lateralis (RE) and gastrocnemius (Run) before, immediately after, and 1, 2, 4, 8, 12 and 24 h after exercise. RE increased mRNA of MRF4 (3.7- to 4.5-fold 2–4 h post), MyoD (5.8-fold 8 h post), myogenin (2.6- and 3.5-fold 8–12 h post), HKII (3.6- to 10.5-fold 2–12 h post), and PDK4 (14- to 26-fold 2–8 h post). There were no differences in Myf5, CD36, and CPT1 mRNA levels 0–24 h post-RE. Run increased mRNA of MyoD (5.0- to 8.0-fold), HKII (12- to 16-fold), and PDK4 (32- to 52-fold) at 8–12 h postexercise. There were no differences in MRF4, Myf5, myogenin, CD36 and CPT1 mRNA levels 0–24 h post-Run. These data indicate a myogenic and metabolic gene induction with RE and Run exercise. The timing of the gene induction is variable and generally peaks 4–8 h postexercise with all gene expression not significantly different from the preexercise levels by 24 h postexercise. These data provide basic information for the timing of human muscle biopsy samples for gene-expression studies involving exercise.

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