Ag Nanoparticles Synthesized Using β-Caryophyllene Isolated from Murraya koenigii: Antimalarial (Plasmodium falciparum 3D7) and Anticancer Activity (A549 and HeLa Cell Lines)

This study aimed to investigate the anticancer activity of Haplophyllum tuberculatum(Forsk.) aerial parts ethanol extract and fractions and reveal the potential anticancer targets, binding modes, pharmacokinetics, and toxicity properties of its phytoconstituents. MTT assay was used to investigate the anticancer activity. TargetNet, ChemProt version 2.0, and CLC-Pred web servers were used for virtual screening, and Cresset Flare software was used for molecular docking with the 26 predicted targets. Moreover, pkCSM, swiss ADME, and eMolTox web servers were used to predict pharmacokinetics and safety. Ethanolic extracts of H. tuberculatum on HepG2 and HeLa cell lines showed promising activities with IC50 values 54.12 and 48.1 µg/mL, respectively. Further, ethyl acetate fraction showed the highest cytotoxicity on HepG2 and HeLa cell lines with IC50 values 41.7 and 52.31 µg/mL. Of 70 compounds screened virtually, polygamain, justicidin A, justicidin B, haplotubine, kusunokinin, and flindersine were predicted as safe anticancer drugs candidates. They showed the highest binding scores with targets involved in cell growth, proliferation, survival, migration, tumor suppression, induction of apoptosis, metastasis, and drug resistance. Our findings revealed the potency of H. tuberculatum as a source of anticancer candidates that further studies should support.

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Influence of substituents on DNA and protein binding of cyclometalated Ir(iii) complexes and anticancer activity

Dalton Transactions ◽

10.1039/c7dt01015j ◽

2017 ◽

Vol 46 (26) ◽

pp. 8572-8585 ◽

Cited By ~ 19

Author(s):

Sujay Mukhopadhyay ◽

Roop Shikha Singh ◽

Rajendra Prasad Paitandi ◽

Gunjan Sharma ◽

Biplob Koch ◽

...

Keyword(s):

Protein Binding ◽

Hela Cell ◽

Anticancer Activity ◽

Cell Lines ◽

Binding Studies ◽

Hela Cell Lines ◽

Influence Of Substituents

Three cyclometalated Ir(iii) complexes based on terpyridyl ligands have been efficiently used for DNA and protein binding studies as well as anticancer activity against HeLa cell lines.

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Tri- and tetranuclear RuII–GdIII2 and RuII–GdIII3 d–f heterometallic complexes as potential bimodal imaging probes for MRI and optical imaging

New Journal of Chemistry ◽

10.1039/c5nj03393d ◽

2016 ◽

Vol 40 (5) ◽

pp. 4606-4616 ◽

Cited By ~ 8

Author(s):

A. Nithyakumar ◽

V. Alexander

Keyword(s):

Hela Cell ◽

Contrast Agents ◽

Anticancer Activity ◽

Fluorescence Imaging ◽

Cell Lines ◽

Heterometallic Complexes ◽

Bimodal Imaging ◽

Hela Cell Lines ◽

Imaging Probes

Tri- and tetranuclear RuII–GdII2 and RuII–GdIII3 d–f heterometallic complexes, which function as contrast agents for MRI and as optical probes for fluorescence imaging, are reported. In vitro studies using the HeLa cell lines show that these complexes exhibit anticancer activity.

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Antibacterial and Anticancer Efficacy of Different Parts of Pistacia Integerrima Plant Extracts

10.21203/rs.3.rs-396639/v1 ◽

2021 ◽

Author(s):

Wajeeha Rani ◽

Farhana Maqbool ◽

Zulfiqar A. Bhatti ◽

Jamshed Iqbal ◽

Muhammad Faisal Siddiqui ◽

...

Keyword(s):

Hela Cell ◽

Ethyl Acetate ◽

Anticancer Activity ◽

Cell Lines ◽

Leaf Extract ◽

Diffusion Method ◽

Bark Extract ◽

Hela Cell Lines ◽

Crude Leaf Extract ◽

Different Parts

Abstract This study explored the antibacterial efficacy of Pistacia integerrima gall, leaf and bark in different solvents. Extracts of these parts prepared in ethanol, methanol and distilled water by using rotary evaporator. To check the antibacterial potential of this plant, minimal inhibitory concentrations of each extract analysed by agar well diffusion method against Staphylococcus aureus, E. coli, Proteus vulgaris, Bacillus subtilis and Pseudomonas aeruginosa. For anticancer activity, hexane, chloroform and ethyl acetate fractions of P. integerrima gall, leaf and bark used against human cervical cancer (HeLa) and baby hamster kidney (BHK–21) cell lines. Results showed that maximum zone of inhibition 25mm formed with ethanolic gall extract in 200µL concentration against B. subtilis. P. vulgaris shows resistance to methanol and aqueous extracts but inhibited with ethanolic leaf extract. Among different parts of P. integerrima, n-hexane leaf fraction shown to be most effective against HeLa cell lines with IC50 of 7.45 µg/mL. In case of BHK-21, highest cell inhibition of 46.8% observed with crude leaf extract than ethyl-acetate bark extract (44.9%) of P. integerrima. It is concluded that the effective inhibitor was hexane leaf fraction against HeLa cell lines in which Heneicosane was found (39.7%) which might be responsible for anticancer activity.

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Anticancer natural products from Aspergillus neoniger, an endophyte of Ficus carica

Bulletin of the National Research Centre ◽

10.1186/s42269-021-00536-8 ◽

2021 ◽

Vol 45 (1) ◽

Author(s):

Randa Abdou ◽

Alaa M. Alqahtani ◽

Gouda H. Attia

Keyword(s):

Hela Cell ◽

Anticancer Activity ◽

Cell Lines ◽

Therapeutic Potential ◽

Ficus Carica ◽

Anticancer Activities ◽

Preliminary Screening ◽

Antiproliferative Effects ◽

Hela Cell Lines ◽

Screening Assays

Abstract Background Several plants have not been investigated for their endophytes, such as the medicinal plant Ficus carica for which anticancer activity has been confirmed. The endophyte Aspergillus neoniger was selected for investigation of its metabolites since it exerted anticancer activities in preliminary screening assays. Results Bioactivity-guided chromatographic fractionation was performed on the endophytic extract and resulted in the identification of asperazine and asperazine A through spectroscopic analysis. Moderate cytotoxicity against HeLa cell lines (CC50 = 18.4 µg mL−1) and moderate antiproliferative effects against HUVEC and K-562 cell lines (GI50 = 31.5 and 24.8 µg mL−1, respectively) were observed for asperazine. Asperazine A on the other hand showed weak cytotoxic activity against HeLa cell lines (CC50 = 34.6 µg mL−1) as well as weak cytostatic activities against HUVEC and K-562 cell lines (GI50 = 40.7 and 50.2 µg mL−1, respectively) while no antimicrobial activity was detected for both compounds. Conclusions These results suggest contribution of A. neoniger to the reported anticancer activity of the host plant and provides a new source of anticancer metabolites with therapeutic potential.

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Synthesis of 5-Benzylidine-3-a-carboxy Ethyl Rhodanines and Study of their Anticancer Activity Against HeLa Cell Lines and Antibacterial Activity Against MRSA

Asian Journal of Chemistry ◽

10.14233/ajchem.2013.15467 ◽

2013 ◽

Vol 25 (18) ◽

pp. 10353-10356

Author(s):

Kaveri Sundaram ◽

Subban Ravi

Keyword(s):

Antibacterial Activity ◽

Hela Cell ◽

Anticancer Activity ◽

Cell Lines ◽

Hela Cell Lines

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Evaluation of Antioxidant, Antimicrobial and Anticancer activity of Thiazole Tagged Isatin Hydrazones

JOURNAL OF PHARMACEUTICAL CHEMISTRY ◽

10.14805/jphchem.2016.art52 ◽

2016 ◽

Vol 3 (2) ◽

pp. 4-9 ◽

Cited By ~ 3

Author(s):

Venkateshwarlu Eggadi ◽

Umasankar Kulandaivelu ◽

Sharvana Bhava Bandaru Sheshagiri ◽

Venkateshwar Rao Jupalli

Keyword(s):

Antioxidant Activity ◽

Hela Cell ◽

Cytotoxic Activity ◽

Anticancer Activity ◽

Cell Lines ◽

Wide Spectrum ◽

Zone Of Inhibition ◽

Hela Cell Lines ◽

Ic50 Values ◽

Isatin Derivatives

Isatin and its derivatives is versatile lead molecule for potential bioactive agents and shows wide spectrum of activities. In this study, we evaluated antioxidant, antimicrobial and cytotoxic activity of isatin-3-[N2-(2-benzalaminothiazol-4-yl)] hydrazone derivatives using well defined models. Antioxidant activity of the isatin derivatives (Va-Vj) was evaluated by using the 1, 1-diphenyl-2-picryl hydrazine radicals scavenging assay. The antimicrobial activity is evaluated by cup plate method and anticancer activity is evaluated by MTT assay against HBL-100 & HeLa cell lines. Compound Vh (R = 5-Cl, R1 = OH & R2 = OCH3) showed good antioxidant activity with the IC50 of 8.09 M. In addition Ve and Vi have showned most active antibacterial activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli with a Zone of Inhibition (mm) 20, 16, 18 and 14, 12, 15 on respective organism at 100 g/disc. The compound Vi have produced a good antifungal activity against Aspergillus niger and Clostridium vericulata with the zone of inhibition values of 9 and 8 mm respectively. These isatin derivatives also among the test compounds, compound Vd (R = 5-Cl, R1 = OH & R2 = OCH3) and compound Vh (R = 5-Cl, R1 = OH & R2 = OCH3) have shown nearly equal cytotoxic activity with IC50 values of 246.53 mM and 247.29 mM against HBL-100 cell lines and HeLa cell lines respectively. From the results, isatin derivatives showed powerful antioxidant activity, antimicrobial and anticancer activity may be due to the halogens substituted at 5th position of isatin. The standard drugs used were ampicillin, clotrimazole, cisplatin and ascorbic acid for antibacterial, antifungal, anticancer and antioxidant respectively.

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