Standardization of the 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium, Inner Salt (MTS) Assay for the SK-N-SH, KYSE-30, MCF-7, and HeLa Cell Lines

2006 ◽  
Author(s):  
Jennifer G. Catalano ◽  
James J. Valdes ◽  
Darrel Menking ◽  
Kyle Hubbard ◽  
Averell L. Gnatt
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Hela Cell Lines ◽  
Mts Assay ◽  
Mcf 7

scholarly journals Structures, Chemical Conversions, and Cytotoxicity of Tricholopardins C and D, Two Tricholoma Triterpenoids from the Wild Mushroom Tricholoma pardinum

2021 ◽  
Author(s):  
Chen Shi ◽  
Yue-Ling Peng ◽  
Juan He ◽  
Zheng-Hui Li ◽  
Ji-Kai Liu ◽  
...  
Keyword(s):  
Hela Cell ◽  
Single Crystal ◽  
Cell Lines ◽  
Cell Apoptosis ◽  
Spectroscopic Methods ◽  
Wild Mushroom ◽  
X Ray Diffraction ◽  
X Ray ◽  
Hela Cell Lines ◽  
Mcf 7

AbstractTwo undescribed Tricholoma triterpenoids, namely tricholopardins C (1) and D (2), were isolated from the wild mushroom Tricholoma pardinum. Their structures with absolute configurations were elucidated by spectroscopic methods, as well as the single crystal X-ray diffraction. Compounds 1 and 2 were further obtained by chemical conversions from the known analogues. Compound 1 showed significant cytotoxicity to MCF-7 and Hela cell lines with IC50 values of 4.7 μM and 9.7 μM, respectively. Its mechanism of inducing MCF-7 cell apoptosis was studied briefly. Graphical Abstract

Radiation dose rate affects the radiosensitization of MCF-7 and HeLa cell lines to X-rays induced by dextran-coated iron oxide nanoparticles

2017 ◽  
Vol 93 (8) ◽  
pp. 757-763 ◽  
Author(s):  
Karim Khoshgard ◽  
Parvaneh Kiani ◽  
Abbas Haghparast ◽  
Leila Hosseinzadeh ◽  
Mohammad Taghi Eivazi
Keyword(s):  
Iron Oxide ◽  
Radiation Dose ◽  
Hela Cell ◽  
Dose Rate ◽  
Cell Lines ◽  
Oxide Nanoparticles ◽  
X Rays ◽  
Radiation Dose Rate ◽  
Hela Cell Lines ◽  
Mcf 7

scholarly journals In Vitro, Evaluation of Anticancer and Anti-dermatophytes Activities of Some Egyptian Medicinal Plants 

2020 ◽  
Author(s):  
Esraa Ahmed Mohamed El-Bondkly ◽  
Mervat Morsy El-Gendy ◽  
Aya Ahmed Mohamed El-Bondkly ◽  
Fareed Shawky El-Shenawy ◽  
Alaa Ahmed Mohamed El-Bondkly
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Methanolic Extract ◽  
Acetone Extract ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Hela Cell Lines ◽  
Trichophyton Soudanense ◽  
Hct 116 ◽  
Mcf 7

Abstract In this investigation three ancient Egyptian medical plants; Plantago albicans L., Thymelaea hirsuta (L.) Endl. and Urginea maritima (L.) were chosen to explore their biochemical properties, anticancer and antimycotic activities against clinical dermatophytes. Growing of Trichophyton soudanense, Trichophyton erinacei, Microsporum audouinii, Microsporum gypseum, Microsporum gallinae, Microsporum ferrogenium, Microsporum cookie, Microsporum racemosum, Microsporum persicolor and Microsporum canis were totally inhibited by the 100 µg/mL of P. albicans methanolic extract. Whereas the growth of T. soudanense, T. erinacei, Trichophyton rubrum, Trichophyton tonsurance and Trichophyton mentagrophytes and Epidermophyton floccosum were 100% inhibited by the methanolic extract of T. hirsiuta at 100 µg/mL concentration, however its acetone extract was more active against Microsporum species. The highest fungi toxicity against all dermatophytic fungi was detected in both methanol and acetone extract of U. maritima at a concentration ranged between 50 to 100 µg/mL. Methanolic extract of P. albicans inhibited the viability of HCT-116, HepG-2, MCF-7 and HeLa cell lines by (41%, 40%, 80% and 81%); (30%, 20%, 66% and 70%); (19%, 0%, 49% and 57%); (5%, 0%, 35% and 45%) and (0%, 0%, 21% and 40%) at 25, 50, 100, 200 and 300 µg/mL, respectively. Moreover, the cells death after treatment with the same concentrations of T. hirsuta methanol extract were (10%, 20%, 23% and 60%); (16%, 40%, 58% and 80%); (40%, 50%, 80% and 100%); (51%, 65%, 100% and 100%) and (49%, 82%, 100% and 100%) in HCT-116, HepG-2, MCF-7 and HeLa cells, respectively. Viability of HCT-116, MCF-7 and HepG-2 cell lines was totally suppressed with 100 µg/mL whereas the HeLa cell growth was reduced to 10% and totally killed at 100 and 200 µg/mL, respectively of U. maritima methanolic extract. All data approve the significance of Egyptian ethnomedical plants as potent source of diverse bioactive pharmaceutical metabolites.

Unpredicted formation of copper(ii) complexes containing 2-thiophen-2-yl-1-thiophen-2-ylmethyl-1H-benzoimidazole and their most promising in vitro cytotoxicity in MCF-7 and HeLa cell lines over cisplatin

2017 ◽  
Vol 41 (17) ◽  
pp. 8885-8898 ◽  
Author(s):  
P. Naveen ◽  
Ruchi Jain ◽  
P. Kalaivani ◽  
R. Shankar ◽  
F. Dallemer ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Sodium Azide ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Mcf 7

An in situ reaction of CuCl2·2H2O, o-phenylenediamine, thiophene-2-carbaldehyde and sodium azide in methanol afforded complex 1a.

Anticancer effect of A-ring or/and C-ring modified oleanolic acid derivatives on KB, MCF-7 and HeLa cell lines

2012 ◽  
Vol 10 (11) ◽  
pp. 2201 ◽  
Author(s):  
Barbara Bednarczyk–Cwynar ◽  
Lucjusz Zaprutko ◽  
Piotr Ruszkowski ◽  
Bogusław Hładoń
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Oleanolic Acid ◽  
Anticancer Effect ◽  
Hela Cell Lines ◽  
Mcf 7

Effect of black seeds (Nigella sativa) volatile oil on the cervical cancer: In vitro study, on Hela cell lines

2019 ◽  
Vol 7 (4) ◽  
pp. 91-96
Author(s):  
Isra'a Al-sobhi ◽  
◽  
Rawan Al-Ghabban ◽  
Soad Shaker Ali ◽  
Jehan Al-Amri ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cell Lines ◽  
Nigella Sativa ◽  
In Vitro Study ◽  
Volatile Oil ◽  
Vitro Study ◽  
Hela Cell Lines ◽  
Black Seeds

Cytotoxic effects of C-glycosides in HOS and HeLa cell lines

2007 ◽  
Vol 17 (13) ◽  
pp. 3676-3681 ◽  
Author(s):  
Carlos A. Sanhueza ◽  
Carlos Mayato ◽  
Rubén P. Machı´n ◽  
José M. Padrón ◽  
Rosa L. Dorta ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Cytotoxic Effects ◽  
Hela Cell Lines
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