scholarly journals In-vitro Cytotoxicity and In-silico Insights of the Multi-target Anticancer Candidates from Haplophyllum tuberculatum

2021 ◽  
Vol 4 (3) ◽  
pp. 192-201
Author(s):  
Mosab Yahya Al-Nour ◽  
Ahmed H Arbab ◽  
Mohammad Khalid Parvez ◽  
Arwa Y Mohamed ◽  
Mohammed S Al-Dosari
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Binding Modes ◽  
Web Servers ◽  
Hela Cell Lines ◽  
Ic50 Values ◽  
Haplophyllum Tuberculatum

This study aimed to investigate the anticancer activity of Haplophyllum tuberculatum(Forsk.) aerial parts ethanol extract and fractions and reveal the potential anticancer targets, binding modes, pharmacokinetics, and toxicity properties of its phytoconstituents. MTT assay was used to investigate the anticancer activity. TargetNet, ChemProt version 2.0, and CLC-Pred web servers were used for virtual screening, and Cresset Flare software was used for molecular docking with the 26 predicted targets. Moreover, pkCSM, swiss ADME, and eMolTox web servers were used to predict pharmacokinetics and safety. Ethanolic extracts of H. tuberculatum on HepG2 and HeLa cell lines showed promising activities with IC50 values 54.12 and 48.1 µg/mL, respectively. Further, ethyl acetate fraction showed the highest cytotoxicity on HepG2 and HeLa cell lines with IC50 values 41.7 and 52.31 µg/mL. Of 70 compounds screened virtually, polygamain, justicidin A, justicidin B, haplotubine, kusunokinin, and flindersine were predicted as safe anticancer drugs candidates. They showed the highest binding scores with targets involved in cell growth, proliferation, survival, migration, tumor suppression, induction of apoptosis, metastasis, and drug resistance. Our findings revealed the potency of H. tuberculatum as a source of anticancer candidates that further studies should support.

Tri- and tetranuclear RuII–GdIII2 and RuII–GdIII3 d–f heterometallic complexes as potential bimodal imaging probes for MRI and optical imaging

2016 ◽  
Vol 40 (5) ◽  
pp. 4606-4616 ◽  
Author(s):  
A. Nithyakumar ◽  
V. Alexander
Keyword(s):  
Hela Cell ◽  
Contrast Agents ◽  
Anticancer Activity ◽  
Fluorescence Imaging ◽  
Cell Lines ◽  
Heterometallic Complexes ◽  
Bimodal Imaging ◽  
Hela Cell Lines ◽  
Imaging Probes

Tri- and tetranuclear RuII–GdII2 and RuII–GdIII3 d–f heterometallic complexes, which function as contrast agents for MRI and as optical probes for fluorescence imaging, are reported. In vitro studies using the HeLa cell lines show that these complexes exhibit anticancer activity.

scholarly journals In vitro Cytotoxicity Studies of Zn (Zinc) Nanoparticles Synthesized from Abutilon indicum L. against Human Cervical Cancer (HeLa) Cell Lines.

2016 ◽  
Vol 8 (2) ◽  
pp. 127-131 ◽  
Author(s):  
Badarinath Druvarao Kulkarni ◽  
Samim Sultana ◽  
Mayuri Bora ◽  
Ishita Dutta ◽  
Padmaa Milaap Paarakh ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Zinc Nanoparticles ◽  
Cytotoxicity Studies ◽  
Hela Cell Lines ◽  
Human Cervical Cancer ◽  
Abutilon Indicum

Evaluation of Antioxidant, Antimicrobial and Anticancer activity of Thiazole Tagged Isatin Hydrazones

2016 ◽  
Vol 3 (2) ◽  
pp. 4-9 ◽  
Author(s):  
Venkateshwarlu Eggadi ◽  
Umasankar Kulandaivelu ◽  
Sharvana Bhava Bandaru Sheshagiri ◽  
Venkateshwar Rao Jupalli
Keyword(s):  
Antioxidant Activity ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Wide Spectrum ◽  
Zone Of Inhibition ◽  
Hela Cell Lines ◽  
Ic50 Values ◽  
Isatin Derivatives

Isatin and its derivatives is versatile lead molecule for potential bioactive agents and shows wide spectrum of activities. In this study, we evaluated antioxidant, antimicrobial and cytotoxic activity of isatin-3-[N2-(2-benzalaminothiazol-4-yl)] hydrazone derivatives using well defined models. Antioxidant activity of the isatin derivatives (Va-Vj) was evaluated by using the 1, 1-diphenyl-2-picryl hydrazine radicals scavenging assay. The antimicrobial activity is evaluated by cup plate method and anticancer activity is evaluated by MTT assay against HBL-100 & HeLa cell lines. Compound Vh (R = 5-Cl, R1 = OH & R2 = OCH3) showed good antioxidant activity with the IC50 of 8.09 M. In addition Ve and Vi have showned most active antibacterial activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli with a Zone of Inhibition (mm) 20, 16, 18 and 14, 12, 15 on respective organism at 100 g/disc. The compound Vi have produced a good antifungal activity against Aspergillus niger and Clostridium vericulata with the zone of inhibition values of 9 and 8 mm respectively. These isatin derivatives also among the test compounds, compound Vd (R = 5-Cl, R1 = OH & R2 = OCH3) and compound Vh (R = 5-Cl, R1 = OH & R2 = OCH3) have shown nearly equal cytotoxic activity with IC50 values of 246.53 mM and 247.29 mM against HBL-100 cell lines and HeLa cell lines respectively. From the results, isatin derivatives showed powerful antioxidant activity, antimicrobial and anticancer activity may be due to the halogens substituted at 5th position of isatin. The standard drugs used were ampicillin, clotrimazole, cisplatin and ascorbic acid for antibacterial, antifungal, anticancer and antioxidant respectively.

Unpredicted formation of copper(ii) complexes containing 2-thiophen-2-yl-1-thiophen-2-ylmethyl-1H-benzoimidazole and their most promising in vitro cytotoxicity in MCF-7 and HeLa cell lines over cisplatin

2017 ◽  
Vol 41 (17) ◽  
pp. 8885-8898 ◽  
Author(s):  
P. Naveen ◽  
Ruchi Jain ◽  
P. Kalaivani ◽  
R. Shankar ◽  
F. Dallemer ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Lines ◽  
Sodium Azide ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Mcf 7

An in situ reaction of CuCl2·2H2O, o-phenylenediamine, thiophene-2-carbaldehyde and sodium azide in methanol afforded complex 1a.

scholarly journals Design, Synthesis, and Anticancer Screening for Repurposed Pyrazolo[3,4-d]pyrimidine Derivatives on Four Mammalian Cancer Cell Lines

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2961
Author(s):  
Eman M. Othman ◽  
Amany A. Bekhit ◽  
Mohamed A. Anany ◽  
Thomas Dandekar ◽  
Hanan M. Ragab ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
A549 Cells ◽  
Design Synthesis ◽  
In Silico Docking ◽  
Hela Cell Lines ◽  
Ic50 Values ◽  
High Cytotoxic Activity

The present study reports the synthesis of new purine bioisosteres comprising a pyrazolo[3,4-d]pyrimidine scaffold linked to mono-, di-, and trimethoxy benzylidene moieties through hydrazine linkages. First, in silico docking experiments of the synthesized compounds against Bax, Bcl-2, Caspase-3, Ki67, p21, and p53 were performed in a trial to rationalize the observed cytotoxic activity for the tested compounds. The anticancer activity of these compounds was evaluated in vitro against Caco-2, A549, HT1080, and Hela cell lines. Results revealed that two (5 and 7) of the three synthesized compounds (5, 6, and 7) showed high cytotoxic activity against all tested cell lines with IC50 values in the micro molar concentration. Our in vitro results show that there is no significant apoptotic effect for the treatment with the experimental compounds on the viability of cells against A549 cells. Ki67 expression was found to decrease significantly following the treatment of cells with the most promising candidate: drug 7. The overall results indicate that these pyrazolopyrimidine derivatives possess anticancer activity at varying doses. The suggested mechanism of action involves the inhibition of the proliferation of cancer cells.

Effect of black seeds (Nigella sativa) volatile oil on the cervical cancer: In vitro study, on Hela cell lines

2019 ◽  
Vol 7 (4) ◽  
pp. 91-96
Author(s):  
Isra'a Al-sobhi ◽  
◽  
Rawan Al-Ghabban ◽  
Soad Shaker Ali ◽  
Jehan Al-Amri ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cell Lines ◽  
Nigella Sativa ◽  
In Vitro Study ◽  
Volatile Oil ◽  
Vitro Study ◽  
Hela Cell Lines ◽  
Black Seeds

scholarly journals The cytotoxic activity of pine needles ethanolic extract of Pinus merkusii on HeLa cell lines

2021 ◽  
Vol 33 ◽  
pp. 03001
Author(s):  
Annise Proboningrat ◽  
Amaq Fadholly ◽  
Sri Agus Sudjarwo ◽  
Fedik Abdul Rantam ◽  
Agung Budianto Achmad
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Anticancer Agent ◽  
Ethanolic Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Lines ◽  
Cytotoxicity Assessment ◽  
Pinus Merkusii

Several efforts have been made to discover new anticancer agents based on natural ingredients. Meanwhile, previous studies have shown that different Pine genus species exhibit cytotoxic activity against various types of cancer cells. This plant is rich in phenolic compounds, especially procyanidins, flavonoids, and phenolic acids. Therefore, this study aims to investigate the in vitro cytotoxicity of Pinus merkusii needles extract on HeLa cancer cell lines. The cytotoxicity assessment was measured using MTT assay and expressed as IC50 value. The results showed that the ethanolic extract poses a dose and time-dependent cytotoxic activity with an IC50 value of 542.5 µg/ml at 48 hours of incubation. Based on this result, Pinus merkusii needles’ ethanolic extract has the potential of a novel candidate for an anticancer agent.

Six New neo-Clerodane Diterpenoids from Aerial Parts of Scutellaria barbata and Their Cytotoxic Activities

Planta Medica ◽  
2018 ◽  
Vol 84 (17) ◽  
pp. 1292-1299 ◽  
Author(s):  
Guo-Chun Yang ◽  
Jia-Hui Hu ◽  
Bing-Long Li ◽  
Huan Liu ◽  
Jia-Yue Wang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cancer ◽  
In Vitro Cytotoxicity ◽  
Cytotoxic Activities ◽  
Scutellaria Barbata ◽  
Human Cancer Cell Lines ◽  
Aerial Parts ◽  
Ic50 Values ◽  
Clerodane Diterpenoids

AbstractSix new neo-clerodane diterpenoids (1–6), scutebatas X – Z, A1-C1, along with twelve known ones (7–18) were obtained via the phytochemical investigation of the aerial parts of Scutellaria barbata. Their structures were established by detailed spectroscopic analysis. The absolute configurations of 1 and 2, as the representative members of this type, were identified based on a circular dichroic exciton chirality method. Moreover, in vitro cytotoxicity of compounds 1–6 were evaluated against three human cancer cell lines (SGC-7901, MCF-7, and A-549) using the MTT method. Compound 6 showed cytotoxic activities against all the three cell lines with IC50 values of 17.9, 29.9, and 35.7 µM, respectively.

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