Production of Soluble and Functional Anti-TNF-α Fab' Fragment in Cytoplasm of E. coli: Investigating the Effect of Process Conditions on Cellular Biomass and Protein Yield Using Response Surface Methodology

2021 ◽  
Author(s):  
Andisheh Talaei ◽  
Somayeh Mazaheri ◽  
Elham Bayat ◽  
Behnaz Bakhshandeh ◽  
Masoumeh Sabzalinejad ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Protein Yield ◽  
Process Conditions ◽  
Fab Fragment ◽  
E Coli ◽  
Tnf Α

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

scholarly journals Optimization of osmotic dehydration of chestnut (Castanea sativa Mill.) slices using Response Surface Methodology

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Teresa Delgado ◽  
Bruna Paim ◽  
José Alberto Pereira ◽  
Susana Casal ◽  
Elsa Ramalhosa
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Water Loss ◽  
Osmotic Dehydration ◽  
Sucrose Concentration ◽  
Volume Ratio ◽  
Castanea Sativa ◽  
Color Variation ◽  
Process Conditions ◽  
Operational Conditions

Osmotic dehydration of chestnut slices in sucrose was optimized for the first time by Response Surface Methodology (RSM). Experiments were planned according to a three-factor central composite design (α=1.68), studying the influence of sucrose concentration, temperature and time, on the following parameters: volume ratio, water activity, color variation, weight reduction, solids gain, water loss and normalized moisture content, as well as total moisture, ash and fat contents. The experimental data was adequately fitted into second-order polynomial models with coefficients of determination (R2) from 0.716 to 0.976, adjusted-R2 values from 0.460 to 0.954, and non-significant lacks of fit. The optimal osmotic dehydration process conditions for maximum water loss and minimum solids gain and color variation were determined by the “Response Optimizer” option: 83% sucrose concentration, 20 °C and 9.2 hours. Thus, the best operational conditions corresponded to high sugar concentration and low temperature, improving energy saving and decreasing the process costs.

Improvement of the process of removing phosphorus from high-phosphorus distillery effluent by ferric chloride using response surface methodology and three-step method

2019 ◽  
Vol 79 (11) ◽  
pp. 2046-2055
Author(s):  
Shengjian Li ◽  
Ting Lei ◽  
Fang Jiang ◽  
Changlin Miao ◽  
Xiangjun Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ferric Chloride ◽  
Quadratic Model ◽  
Phosphorus Concentration ◽  
Process Conditions ◽  
Distillery Effluent ◽  
Step Method ◽  
Standard Design ◽  
Major Factors

Abstract The purpose of this study was to optimize the coagulation–flocculation effect of a wastewater treatment system using the response surface methodology (RSM) and three-step method to minimize phosphorus concentration in the distillate wastewater. In order to minimize the concentration of total phosphorus (TP), experiments were carried out using -factorial designs with three levels and three factors. A Box–Behnken design, which is the standard design of RSM, was used to evaluate the effects and interactions of three major factors (Fe:P (w/w) ratio, coagulation pH and fast mixing speed (FMS)) on the treatment efficiency. A multivariable quadratic model developed for studying the response indicated that the values for optimum conditions for Fe:P (w/w) ratio, coagulation pH and FMS were 2.40, 6.48 and 100 rev min−1, respectively. Under optimal process conditions, the TP concentration in the distillery effluent was reduced from 10 mg L−1 to 0.215 mg L−1, representing a removal efficiency of 97.85%. Based upon the statistical evaluation of results, it is inferred that RSM can be used as an appropriate approach to optimize the coag-flocculation process. Meanwhile, the study has shown that, for the equivalent dose of ferric chloride, the average three-step effect is better than that of the one-time addition.

scholarly journals Development of a Novel Whey Date Beverage Fermented with Kefir Grains Using Response Surface Methodology

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Sana M’hir ◽  
Kais Rtibi ◽  
Asma Mejri ◽  
Manel Ziadi ◽  
Hajer Aloui ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Process Conditions ◽  
Whey Permeate ◽  
Overall Acceptability ◽  
Date Syrup ◽  
Kefir Grains

The aim of this study was to develop a novel kefir beverage using date syrup, whey permeate, and whey. The levels of the kefir grain inoculum (2–5% w/v), fruit syrup (10–50% w/v), and whey permeate (0–5% w/v) on pH, total phenolic content, antioxidant activity, lactic acid bacteria and yeast counts, and overall acceptability were investigated using central composite design. The use of response surface methodology allowed us to obtain a formulation with acceptable organoleptic properties and high antioxidant activities. The obtained beverages had total phenolic content, % DPPH scavenging activity, and overall acceptability ranging from 24 to 74 mg GAE/mL, from 74.80 to 91.37 mg GAE/mL, and from 3.50 to 6 mg GAE/mL (based on a 1 to 9 preference scale), respectively. Date syrup of 36.76% (w/v), whey permeates of 2.99%, and kefir grains inoculum size of 2.08% were the optimized process conditions achieved.

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