Rapid determination of 89,90Sr in wide range of activity concentration by combination of yttrium, strontium separation and Cherenkov counting

2011 ◽  
Vol 292 (2) ◽  
pp. 555-569 ◽  
Author(s):  
Željko Grahek ◽  
Gorana Karanović ◽  
Marijana Nodilo
Keyword(s):  
Activity Concentration ◽  
Rapid Determination ◽  
Cherenkov Counting ◽  
Wide Range ◽  
Strontium Separation

scholarly journals Rapid Determination of Ethylene Oxide and 75 VOCs in Ambient Air with Canister Sampling and Associated Growth Issues

Separations ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 35
Author(s):  
Jason Hoisington ◽  
Jason S. Herrington
Keyword(s):  
Ethylene Oxide ◽  
Rapid Determination ◽  
Complex Mixture ◽  
Ambient Air ◽  
Gas Chromatography Mass Spectrometry ◽  
Average Accuracy ◽  
Relative Standard ◽  
Wide Range ◽  
Us Epa

A canister-based sampling method along with preconcentrator-Gas chromatography-Mass Spectrometry (GC-MS) analysis was applied to ethylene oxide (EtO or EO) and 75 other volatile organic compounds (VOCs) in ambient air. Ambient air can contain a large variety of VOCs, and thorough analysis requires non-discriminatory sampling and a chromatographic method capable of resolving a complex mixture. Canister collection of whole air samples allows for the collection of a wide range of volatile compounds, while the simultaneous analysis of ethylene oxide and other VOCs allows for faster throughput than separate methods. The method presented is based on US EPA Method TO-15A and allows for the detection of EtO from 18 to 2500 pptv. The method has an average accuracy of 104% and precision of 13% relative standard deviation (RSD), with an instrument run time of 32 min. In addition, a link between canister cleanliness and ethylene oxide growth is observed, and potential mechanisms and cleaning strategies are addressed.

Optimized liquid-chromatographic determination of metronidazole and its metabolites in plasma.

1984 ◽  
Vol 30 (5) ◽  
pp. 784-787 ◽  
Author(s):  
R A Gibson ◽  
L Lattanzio ◽  
H McGee
Keyword(s):  
Rapid Determination ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Detector Response ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatographic Method ◽  
Wide Range ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract Metronidazole and its known metabolites in plasma can be rapidly separated by a "high-pressure" liquid-chromatographic method that can also be adapted for rapid determination of tinidazole. Samples deproteinized with trichloroacetic acid (50 g/L final concentration) undergo isocratic separation on a reversed-phase C18 column eluted with an 8/92 (by vol) mixture of acetonitrile/KH2PO4 (5 mmol/L, pH 3.0). The method is sensitive, reliably detecting as little as 25 micrograms of metronidazole and (or) its metabolites per milliliter of plasma. The detector response varied linearly with concentration for all compounds tested over a wide range (25-500 micrograms/L). Within-day and between-day variation was generally less than 2.5% for all concentrations of all compounds tested. Various other antibiotics tested did not interfere.

Simple, rapid determination of serum guanase activity with the Hitachi 736 automated discrete analyzer.

1985 ◽  
Vol 31 (1) ◽  
pp. 103-105 ◽  
Author(s):  
Y Nishikawa ◽  
K Fukumoto ◽  
F Watanabe
Keyword(s):  
Superoxide Dismutase ◽  
Xanthine Oxidase ◽  
Activity Concentration ◽  
Rapid Determination ◽  
Kinetic Method ◽  
Reference Interval ◽  
New Method ◽  
Triton X ◽  
Healthy Persons

Abstract In this new method for determining serum guanase activity by use of the Hitachi 736-40 automated analyzer, serum is incubated with a mixture of xanthine oxidase, superoxide dismutase, and catalase; a reagent containing KCN, guanine, nitrotetrazolium blue, and Triton X-100 is added; and the increase in absorbance at 570 and 660 nm is measured for 2.4 min. Only 20 microL of sample is required, and results are linearly related to the activity concentration of guanase up to 30 U/L. Within-run and day-to-day precision (CV) was respectively 2.6 to 4.2% and 3.5 to 5.5% over 0-30 U of guanase activity per liter. The normal reference interval, as calculated from data on 40 healthy persons, is 0.1 to 2.2 U/L. Results correlate well (r = 0.997) with those by a kinetic method (Clin Chem 27: 560, 1981). The guanase activity of 150 samples can be measured within 1 h by this method.

Clinical Nephelometry: 1. New, Accurate, Heat-Precipitation Nephelometric Method for Rapid Determination of Plasma Fibrinogen

1972 ◽  
Vol 18 (1) ◽  
pp. 73-75 ◽  
Author(s):  
Eugene W Rice ◽  
D E R Muesse
Keyword(s):  
Linear Response ◽  
Clinical Utility ◽  
Rapid Determination ◽  
Plasma Fibrinogen ◽  
Biochemical Screening ◽  
Nephelometric Method ◽  
Wide Range ◽  
Accurate Procedure

Abstract We have developed an accurate procedure for measurement of plasma fibrinogen. Turbidity resulting from heating (56°C, 20 min) plasma diluted with a new "zwitterionic" buffered-saline reagent (pH 5.6) is nephelometrically measured (600 nm). We evaluated the method both by confirming the accuracy of its prestandardization and calibration on a new clinical nephelometer, and by comparative standardization by use of a conventional spectrophotometer. The nephelometer is more sensitive and gives a linear response over a wide range of concentrations. Representative information supporting the broad clinical utility of the determination of plasma fibrinogen is illustrated. These assays should be a significant complement to muititest biochemical screening of both healthy and hospitalized individuals.

scholarly journals   Near infrared spectroscopy for deoxynivalenol content estimation in intact wheat grain

2012 ◽  
Vol 58 (No. 4) ◽  
pp. 196-203 ◽  
Author(s):  
V. Dvořáček ◽  
A. Prohasková ◽  
J. Chrpová ◽  
L. Štočková
Keyword(s):  
Near Infrared ◽  
Rapid Determination ◽  
Nir Spectroscopy ◽  
Resistance Breeding ◽  
Partial Least Square ◽  
Least Square ◽  
Prediction Errors ◽  
Wheat Grain ◽  
Wide Range

Non-invasive determination of deoxynivalenol (DON) still presents a challenging problem. Therefore, the present study was aimed at a rapid determination of DON in whole wheat grain by means of FT-NIR spectroscopy, with a wide range of concentrations for potential applications in breeding programs and common systems of quality management using partial least square calibration (PLS) and discriminant analysis technique (DA). Using a set of artificially infected wheat samples with a known content of DON, four PLS models with different concentration range were created. The broadest model predicting DON in the concentration range of 0&ndash;90 mg/kg possessed the highest correlation coefficients of calibration and cross validation (0.94 and 0.88); but also possessed the highest prediction errors (SEP = 6.23 mg/kg). Thus the subsequent combination of DA as the wide range predictive model and the low-range PLS model was used. This technique gave more precise results in the samples with lower DON concentrations &ndash; below 30 mg/kg (SEP = 2.35 mg/kg), when compared to the most wide-range PLS model (SEP = 5.95 mg/kg).<br />Such technique enables to estimate DON content in collections of artificially infected wheat plants in Fusarium resistance breeding experiments. &nbsp;

scholarly journals Optimized and Validated DBS/MAE/LC–MS Method for Rapid Determination of Date-Rape Drugs and Cocaine in Human Blood Samples—A New Tool in Forensic Analysis

Separations ◽  
2021 ◽  
Vol 8 (12) ◽  
pp. 249
Author(s):  
Paweł Stelmaszczyk ◽  
Ewa Gacek ◽  
Renata Wietecha-Posłuszny
Keyword(s):  
Date Rape ◽  
Rapid Determination ◽  
Extraction Process ◽  
Forensic Analysis ◽  
Blood Samples ◽  
Assisted Extraction ◽  
Wide Range ◽  
Human Blood Samples ◽  
Date Rape Drugs

The aim of this work was to develop a new method for the determination of selected substances from the date-rape drugs group: ketamine, benzodiazepines and cocaine. The method is based on the dried blood spot method which seems to be a suitable tool in the analysis of tested substances. The extraction process based on microwave-assisted extraction was optimized to enable optimal conditions for the isolation of a wide range of analytes from blood samples collected on DBS cards. The extraction with ethyl acetate with a buffer of pH = 9 carried out at a temperature of 50 °C for 15 min ensured high extraction efficiency of the tested analytes. The optimized method was validated. Limits of detection (LOD = 4.38–21.1 ng/mL) and quantification (LOQ = 14.6–70.4 ng/mL), inter- and intra-day precision (CV = 1.37–13.4% and 3.39–14.8%, respectively), recovery (RE = 93.0–112.4%) and matrix effect (ME = 98.4–101.6%) were determined. The validation results indicate the possibility of using the proposed method in the analysis of real blood samples collected from victims of sexual assault.

Determination of Serum Physiological Concentration of Methylmalonic Acid by Gas Chromatography-Mass Spectrometry with Selected Ion Monitoring

1998 ◽  
Vol 35 (5) ◽  
pp. 633-636 ◽  
Author(s):  
Nader Rifai ◽  
Thilo Hagen ◽  
Loetta Bradley ◽  
Masayuki Sakamoto
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Methylmalonic Acid ◽  
Solid Phase ◽  
Rapid Determination ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Chromatography Mass Spectrometry ◽  
Wide Range

We developed a sensitive assay for the rapid determination of serum methylmalonic acid concentration using capillary gas chromatography-mass spectrometry (GC/MS) with selected ion monitoring and a simple solid-phase extraction. The assay was linear up to 10 000 nmol/L and had a detection limit < 50 nmol/L, average recovery of 98% and between-day coefficient of variation at concentrations of 570 and 2206 nmol/L of 7.7% and 5.4%, respectively ( n = 25). Comparison with another validated GC/MS method using sera with a wide range of methylmalonic acid concentrations (94-2020 nmol/L) revealed a slope and intercept of 0.97 and 17 nmol/L, respectively ( n = 38). Methylmalonic acid concentrations determined by this assay in a group of apparently healthy individuals ranged from 64–331 nmol/L ( n = 81). We conclude that the method is ideally suited for the determination of methylmalonic acid at physiological concentrations in both clinical and research laboratories.

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