Simple and efficient radiolabeling of hyaluronic acid and its in vivo evaluation via oral administration

PURPOSE. Find a novel delivery system for oral administration of drugs that have absorption window in the upper part of gastrointestinal (GI) track. METHODS. Dipyridamole was chosen as the model drug. A novel system, which combined the osmotic pump controlled release system and the floating system, was designed; matrix tablets (MT) were prepared for compares. The effects of pH, temperature and hydrodynamic conditions on drug release and the floating behavior of floating osmotic pump system (FOP) were investigated. In vivo evaluation was performed by a three-crossover study in six Beagle dogs relative to the conventional tablet (CT). Cumulative percent input in vivo was compared with that of in vitro release profiles. RESULTS. Floating behavior of FOP, drug releases from FOP and MT were sensitive to pH of dissolution media but not sensitive to temperature; the release of dipyridamole from MT was influenced by stirring rate while drug release from FOP was not. AUC of FOP was larger than MT and CT. The linear correlations between fraction absorbed in vivo and fraction dissolved in vitro was established for FOP-a true zero-order release formula, whereas only a nonlinear correlation was obtained for MT. CONCLUTIONS. FOP could be a novel way for the oral administration for drugs like dipyridamole.

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Oral Intake of Collagen Peptide Attenuates Ultraviolet B Irradiation-Induced Skin Dehydration In Vivo by Regulating Hyaluronic Acid Synthesis

International Journal of Molecular Sciences ◽

10.3390/ijms19113551 ◽

2018 ◽

Vol 19 (11) ◽

pp. 3551 ◽

Cited By ~ 3

Author(s):

Min Kang ◽

Silvia Yumnam ◽

Sun Kim

Keyword(s):

Hyaluronic Acid ◽

Oral Administration ◽

Protein Expression ◽

Ultraviolet B ◽

Skin Hydration ◽

Skin Barrier Function ◽

Hairless Mice ◽

Uvb Irradiation ◽

Collagen Peptide

Collagen peptide (CP) has beneficial effects on functions of the skin, such as skin barrier function and skin elasticity, in vivo. However, there are few studies investigating the mechanism underlying the potential effects of CP in skin epidermal moisturization after ultraviolet B (UVB) irradiation. In this study, we examined whether orally-administered CP affects the loss of skin hydration induced by UVB irradiation in hairless mice. SKH-1 hairless mice were orally administered CP at two doses (500 and 1000 mg/kg) for nine weeks, and the dorsal skin was exposed to UVB. The potential effects of CP were evaluated by measuring the transepidermal water loss (TEWL), skin hydration, wrinkle formation, and hyaluronic acid expression in the dorsal mice skin. We found that oral administration of CP increased skin hydration and decreased wrinkle formation compared to the UVB-irradiated group. Treatment of CP increased the mRNA and protein expression of hyaluronic acid synthases (HAS-1 and -2) concomitant with an increased hyaluronic acid production in skin tissue. The expression of hyaluronidase (HYAL-1 and 2) mRNA was downregulated in the CP-treated group. In addition, the protein expression of skin-hydrating factors, filaggrin and involucrin, was upregulated via oral administration of CP. In summary, these results show that oral administration of CP increases hyaluronic acid levels, which decreases during UVB photoaging. Therefore, we suggest that CP can be used as a nutricosmetic ingredient with potential effects on UVB-induced skin dehydration and moisture loss in addition to wrinkle formation.

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CD44 targeting biocompatible and biodegradable hyaluronic acid cross-linked zein nanogels for curcumin delivery to cancer cells: In vitro and in vivo evaluation

Journal of Controlled Release ◽

10.1016/j.jconrel.2018.04.050 ◽

2018 ◽

Vol 280 ◽

pp. 20-30 ◽

Cited By ~ 33

Author(s):

Hae-Yong Seok ◽

N. Sanoj Rejinold ◽

Kamali Manickavasagam Lekshmi ◽

Kondareddy Cherukula ◽

In-Kyu Park ◽

...

Keyword(s):

Hyaluronic Acid ◽

Cancer Cells ◽

In Vivo Evaluation ◽

Cd44 Targeting

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In Vitro and In Vivo Evaluation of a Multifunctional Hyaluronic acid Based Hydrogel System for Local Application on the Retina

Macromolecular Symposia ◽

10.1002/masy.201100049 ◽

2011 ◽

Vol 309-310 (1) ◽

pp. 229-235 ◽

Cited By ~ 4

Author(s):

A. T. Neffe ◽

K. A. Kobuch ◽

M. Maier ◽

N. Feucht ◽

C. P. Lohmann ◽

...

Keyword(s):

Hyaluronic Acid ◽

Local Application ◽

In Vivo Evaluation ◽

Hydrogel System

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Wound Healing: In Vitro and In Vivo Evaluation of a Bio-Functionalized Scaffold Based on Hyaluronic Acid and Platelet-Rich Plasma in Chronic Ulcers

Journal of Clinical Medicine ◽

10.3390/jcm8091486 ◽

2019 ◽

Vol 8 (9) ◽

pp. 1486 ◽

Cited By ~ 12

Author(s):

Barbara De Angelis ◽

Margarida Fernandes Lopes Morais D’Autilio ◽

Fabrizio Orlandi ◽

Giampiero Pepe ◽

Simone Garcovich ◽

...

Keyword(s):

Wound Healing ◽

Hyaluronic Acid ◽

Platelet Rich Plasma ◽

Combined Treatment ◽

Skin Grafting ◽

Control Group ◽

In Vivo Evaluation ◽

Chronic Ulcers

Chronic ulcers are characterized by loss of substance without a normal tendency towards spontaneous healing. The Wound Bed Preparation Guideline advises that after diagnosis, the expert should correct the biological state of the ulcer micro-environment based on TIME principles (Tissue, Infection, Moisture balance, Epidermal). There are many ways to treat such ulcers, for example through use of advanced dressings, negative pressure, surgical toilets, dermal substitutes, autologous skin grafting, and free or local flaps. In vitro and in vivo pre-clinical models hold widely acknowledged potential yet complex limitations. Tissue bioengineering could be an ideal approach to foster innovative strategies in wound healing. Our observational study reports on an in vitro and in vivo evaluation of a bio-functionalized scaffold composed of platelet-rich plasma (PRP) and hyaluronic acid (HA) used in 182 patients affected by chronic ulcers (diabetic and vascular), comparing the results with a control group of 182 patients treated with traditional dressings (HA alone). After 30 days the patients who had undergone the combined treatment (PRP + HA), showed 96.8% ± 1.5% re-epithelialization, as compared to 78.4% ± 4.4% in the control group (HA only). Within 80 days, they had 98.4% ± 1.3% re-epithelialization as compared to 87.8% ± 4.1% in the control group (HA only; p < 0.05). No local recurrence was observed during the follow-up period. PRP + HA treatment showed stronger regenerative potential in terms of epidermal proliferation and dermal renewal compared with HA alone.

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