Nitrosative stress parameters and the level of oxidized DNA bases in patients with multiple sclerosis

10.21203/rs.3.rs-200783/v1 ◽

2021 ◽

Author(s):

Vitalijs Borisovs ◽

Jevgenijs Bodrenko ◽

Jolanta Kalnina ◽

Nikolajs Sjakste

Keyword(s):

Nitric Oxide ◽

Multiple Sclerosis ◽

Nitrosative Stress ◽

Dna Bases ◽

Thiobarbituric Acid Reactive Substance ◽

Healthy Controls ◽

Serum Samples ◽

Oxidative Stress Biomarkers ◽

Modified Dna ◽

Purines And Pyrimidines

Abstract Multiple sclerosis (MS) is a neurodegenerative disease with various factors affecting its etiology. Overproduction of nitric oxide and subsequent lesions of biopolymers are some of the possible causes of the disease. This study aimed to measure the most relevant nitrosative and oxidative stress biomarkers and the level of modified DNA bases in patients with MS. Each parameter was assayed in 25 patients with MS and 25 healthy controls. This study involved detecting blood plasma and serum nitric oxide metabolites by chemiluminescence detector Sievers NOA-280i, malondialdehyde (MDA) measurements with thiobarbituric acid reactive substance (TBARS) assay, detection of oxidized purines and pyrimidines with the enzyme-modified comet assay. Statistical analysis of the results was performed by one-way analysis of variance (ANOVA) and unpaired t test for the comparison of less than three data sets. DNA single-strand breaks, levels of modified purines and pyrimidines, as well as nitrite and nitrate levels in plasma and serum samples, were significantly higher in patients with MS than in healthy controls. On the contrary, MDA levels appeared to be lower in patients with MS.

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Serum Metabolic Profile in Multiple Sclerosis Patients

Multiple Sclerosis International ◽

10.1155/2011/167156 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 26

Author(s):

Barbara Tavazzi ◽

Anna Paola Batocchi ◽

Angela Maria Amorini ◽

Viviana Nociti ◽

Serafina D'Urso ◽

...

Keyword(s):

Multiple Sclerosis ◽

Ascorbic Acid ◽

Uric Acid ◽

Metabolic Profile ◽

Nitrosative Stress ◽

Pathological Condition ◽

Concomitant Increase ◽

Mitochondrial Functions ◽

Multiple Sclerosis Patients ◽

Neuronal Functions

Multiple sclerosis (MS) is a progressive demyelinating process considered as an autoimmune disease, although the causes of this pathology have not been yet fully established. Similarly to other neurodegenerations, MS is characterized by a series of biochemical changes affecting to different extent neuronal functions; great attention has been given to oxidative/nitrosative stress and to alterations in mitochondrial functions. According to previous data, MS patients show significant changes in the circulating concentrations of different metabolites, although it is still unclear whether uric acid undergoes to decrease, increase, or no change under this pathological condition. In this study, we report the serum metabolic profile in terms of purines, pyrimidines, creatinine, malondialdehyde, ascorbic acid, nitrite, and nitrate in a group of 170 MS patients. The results show increase in circulating uric acid and other oxypurines (hypoxanthine and xanthine), as well as in uridine and β-pseudouridine. The concomitant increase in circulating creatinine, malondialdehyde, nitrite, and nitrate, and decrease in ascorbic acid, demonstrates that MS induces alteration in energy metabolism and in oxidants/antioxidants balance that can be monitored in serum of MS patients.

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Nitrosative Stress Molecules in Multiple Sclerosis: A Meta-Analysis

Biomedicines ◽

10.3390/biomedicines9121899 ◽

2021 ◽

Vol 9 (12) ◽

pp. 1899

Author(s):

Moritz Förster ◽

Christopher Nelke ◽

Saskia Räuber ◽

Hans Lassmann ◽

Tobias Ruck ◽

...

Keyword(s):

Multiple Sclerosis ◽

Nitrosative Stress ◽

Neurological Diseases ◽

Meta Analysis ◽

Statistical Significance ◽

Unknown Etiology ◽

Immune Mediated ◽

Pubmed Database ◽

The Central Nervous System ◽

Nitrite Nitrate

Multiple sclerosis (MS) is an immune-mediated disease of the central nervous system of unknown etiology. As it is still a diagnosis of exclusion, there is an urgent need for biomarkers supporting its diagnosis. Increasing evidence suggests that nitrosative stress may play a pivotal role in the pathogenesis of MS. However, previous reports supporting the role of nitrosative stress molecules as disease biomarkers are inconsistent overall. We therefore systematically analyzed the existing literature to compare the serum and cerebrospinal fluid (CSF) levels of nitrite/nitrate in MS patients with those in patients with noninflammatory other neurological diseases (NIOND) and healthy controls (HC), respectively. We searched the PubMed database and included original articles investigating nitrite/nitrate levels in MS patients and NIOND patients or HC based on predefined selection criteria. Effect sizes were estimated by the standardized mean difference using a random effects model. Our results suggest that MS is associated with higher nitrite/nitrate levels within the CSF compared with patients with NIOND (SMD of 1.51; 95% CI: 0.72, 2.30; p = 0.0008). Likewise, nitrite/nitrate in the CSF of MS patients trends towards increased levels compared with those of HC but does not reach statistical significance (SMD of 3.35; 95% CI: −0.48, 7.19; p = 0.07). Measurement of nitrite/nitrate in the CSF might be a valuable tool facilitating the differentiation of MS and NIOND. Further studies with more homogeneous study criteria are needed to corroborate this hypothesis.

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MicroRNAs, Multiple Sclerosis and Depression

International Journal of Molecular Sciences ◽

10.3390/ijms22157802 ◽

2021 ◽

Vol 22 (15) ◽

pp. 7802

Author(s):

Hsiuying Wang

Keyword(s):

Multiple Sclerosis ◽

Depressive Disorders ◽

Nitrosative Stress ◽

Mirna Regulation ◽

Oxidative And Nitrosative Stress ◽

Relapsing Remitting ◽

The Central Nervous System ◽

Patients Experience ◽

Brain And Spinal Cord ◽

The Brain

Multiple sclerosis (MS) is a chronic disease of the central nervous system that affects the brain and spinal cord. There are several disease courses in MS including relapsing–remitting MS (RRMS), primary progressive MS (PPMS), and secondary progressive MS (SPMS). Up to 50% of MS patients experience depressive disorders. Major depression (MD) is a serious comorbidity of MS. Many dysfunctions including neuroinflammation, peripheral inflammation, gut dysbiosis, chronic oxidative and nitrosative stress, and neuroendocrine and mitochondrial abnormalities may contribute to the comorbidity between MS and MD. In addition to these actions, medical treatment and microRNA (miRNA) regulation may also be involved in the mechanisms of the comorbidity between MS and MD. In the study, I review many common miRNA biomarkers for both diseases. These common miRNA biomarkers may help further explore the association between MS and MD.

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Immune-Inflammatory and Oxidative and Nitrosative Stress Biomarkers of Depression Symptoms in Subjects with Multiple Sclerosis: Increased Peripheral Inflammation but Less Acute Neuroinflammation

Molecular Neurobiology ◽

10.1007/s12035-015-9443-4 ◽

2015 ◽

Vol 53 (8) ◽

pp. 5191-5202 ◽

Cited By ~ 32

Author(s):

Ana Paula Kallaur ◽

Josiane Lopes ◽

Sayonara Rangel Oliveira ◽

Andrea Name Colado Simão ◽

Edna Maria Vissoci Reiche ◽

...

Keyword(s):

Multiple Sclerosis ◽

Nitrosative Stress ◽

Depression Symptoms ◽

Peripheral Inflammation ◽

Oxidative And Nitrosative Stress ◽

Stress Biomarkers

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Immune-inflammatory and oxidative and nitrosative stress (IO & NS) pathways in depression and multiple sclerosis (MS): Shared IO & NS pathways but less hyper-acute neuro-inflammation explain the increased incidence of depression in MS

Neurology Psychiatry and Brain Research ◽

10.1016/j.npbr.2015.12.039 ◽

2016 ◽

Vol 22 (1) ◽

pp. 16-17 ◽

Cited By ~ 3

Author(s):

Michael Maes ◽

Ana Paula Kallaur ◽

Josiane Lopes ◽

Sayonara Rangel Oliveira ◽

Andrea Name Colado Simão ◽

...

Keyword(s):

Multiple Sclerosis ◽

Nitrosative Stress ◽

Oxidative And Nitrosative Stress ◽

Neuro Inflammation

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The neutralizing antibody GNbAC1 abrogates HERV-W envelope protein-mediated oligodendroglial maturation blockade

Multiple Sclerosis Journal ◽

10.1177/1352458514560926 ◽

2014 ◽

Vol 21 (9) ◽

pp. 1200-1203 ◽

Cited By ~ 26

Author(s):

David Kremer ◽

Moritz Förster ◽

Tanja Schichel ◽

Peter Göttle ◽

Hans-Peter Hartung ◽

...

Keyword(s):

Multiple Sclerosis ◽

Envelope Protein ◽

Nitrosative Stress ◽

Immune Cell ◽

Neutralizing Antibody ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Stress Reactions ◽

Induce Stress ◽

Inflammatory Reactions

Background: The envelope protein (ENV) of the human endogenous retrovirus type W is implicated in inflammatory reactions in multiple sclerosis (MS) but also interferes with oligodendroglial maturation. A neutralizing antibody GNbAC1 has been developed and successfully been tested in clinical trials. Objectives and methods: We stimulated primary oligodendroglial cells with ENV upon preincubation with GNbAC1 and assessed for nitrosative stress and myelin expression. Results: Neutralization of ENV by GNbAC1 reduces its ability to induce stress reactions resulting in a rescue of myelin expression. Conclusions: Beyond immune cell modulation, this monoclonal antibody may therefore help to overcome the oligodendroglial differentiation blockade in MS.

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Oxidative and Nitrosative Stress in Stable Renal Transplant Recipients with Respect to the Immunosuppression Protocol – Differences or Similarities? / Oksidativni I Nitrozativni Stres U Odnosu Na Imunosupresivni Protokol Kod Pacijenata Sa Stabilnom Funkcijom Presađenog Bubrega – Razlike I Sličnosti

Journal of Medical Biochemistry ◽

10.2478/jomb-2014-0047 ◽

2015 ◽

Vol 34 (3) ◽

pp. 295-303 ◽

Cited By ~ 2

Author(s):

Tatjana Cvetković ◽

Radmila Veličković-Radovanović ◽

Dijana Stojanović ◽

Nikola Stefanović ◽

Aleksandra Ignjatović ◽

...

Keyword(s):

Renal Transplant ◽

Catalase Activity ◽

Nitrosative Stress ◽

Healthy Population ◽

Renal Transplant Recipients ◽

Transplant Recipients ◽

Oxidative And Nitrosative Stress ◽

Sh Groups ◽

Significant Difference ◽

Stress Parameters

SummaryBackground: The aim of the study was to evaluate parameters of oxidative and nitrosative stress as well as antioxidative parameters in a group of renal transplant recipients with stable graft function and no clinical signs of cardiovascular disease. We also aimed to determine the correlations among these parameters and to evaluate potential differences in all the biomarkers with regard to the immunosuppression protocol.Methods: We enrolled 57 renal transplant recipients and 31 controls who were age and sex matched with the renal transplant recipients. All of the patients included in this study had post-renal transplant surgery at least 12 months earlier and were on standard immunosuppressive therapy. In this study, we determined thiobarbituric acid-reactive substances in plasma and red blood cells and advanced oxidation protein products, nitrosative stress parameters (asymmetric and symmetric dimethylarginine - ADMA and SDMA), and antioxidative parameters (total SH groups and catalase activity).Results: The results of our study demonstrated that the levels of oxidative and nitrosative stress were significantly increased compared to the healthy population (p<0.01 except for plasma catalase activity p<0.05). Correlation analysis showed significant positive correlations between: ADMA and SDMA (p<0.01); ADMA and nitrates (p<0.05); SDMA and nitrates (p<0.05); between OS parameters in the experimental group; AOPP and SH groups (p<0.05) and TBARS in plasma and SH groups (p<0.01), SDMA and AOPP (p< 0.05); SDMA and TBARS in plasma (p<0.05); SDMA and SH groups (p<0.01); nitrates and SH groups (p<0.05).Conclusion: There was no significant difference in oxidative and nitrosative stress parameters with respect to the immunosuppressive protocol.

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