Lr1-mediated leaf rust resistance pathways of transgenic wheat lines revealed by a gene expression study using the Affymetrix GeneChip® Wheat Genome Array

In 2002 and 2003, 1,300 isolates of Puccinia recondita f. sp. tritici were obtained from six commercial cultivars of wheat at three locations in the eastern forest-steppe region of Ukraine. All isolates were tested for virulence on an international set of eight differential cultivars. Seventeen known wheat leaf rust races and several new, unnamed races were identified. The most common races in each year were races 61, 149, and 192. In 2003, up to 20 isolates each of the seven most common leaf rust races plus 8 to 10 isolates of unnamed races were tested for virulence to 35 near-isogenic wheat lines with different single Lr genes for leaf rust resistance. Isolates were polymorphic for virulence on Lr1, Lr2a, Lr2b, Lr2c, Lr9, Lr19, Lr23, Lr26, and the combination Lr27 + Lr31. No isolates were found virulent on Lr24, Lr25, or Lr28, and few isolates were virulent on Lr9. Few isolates of known races but most isolates of the new, unnamed races were virulent on Lr19. The 35 Lr gene lines also were exposed to mixed-race inoculum in field plots to tests effectiveness of their resistance. Lines with Lr24, Lr25, and Lr28 suffered no leaf rust damage in the field, and lines with Lr9, Lr18, Lr35, Lr36, and the combination Lr27 + Lr31 showed less than 10% severity.

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Mapping of QTL conferring leaf rust resistance in Chinese wheat lines W014204 and Fuyu 3 at adult plant stage

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(14)60974-6 ◽

2016 ◽

Vol 15 (1) ◽

pp. 18-28 ◽

Cited By ~ 5

Author(s):

Ai-yong QI ◽

Pei-pei ZHANG ◽

Yue ZHOU ◽

Zhan-jun YAO ◽

Zai-feng LI ◽

...

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Adult Plant Stage ◽

Plant Stage ◽

Wheat Lines ◽

Chinese Wheat

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Efficiency of leaf rust resistance genes in Martonvásár

Plant Protection Science ◽

10.17221/10564-pps ◽

2017 ◽

Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽

pp. 593-595

Author(s):

M. Gál ◽

L. Szunics ◽

G. Vida ◽

Lu. Szunics ◽

O. Veisz ◽

...

Keyword(s):

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Agricultural Research ◽

Hungarian Academy ◽

Leaf Rust Resistance ◽

Wheat Lines ◽

Moderately Resistant ◽

Rust Resistance Genes ◽

Leaf Rust Resistance Genes

The efficiency of leaf rust resistance genes in adult plants was studied on near-isogenic lines of Thatcher carrying known leaf rust resistance genes in the artificially inoculated leaf rust nursery of the Agricultural Research Institute of the Hungarian Academy of Sciences in Martonvásár over a five-year period (1997–2001). Eight of the wheat lines tested (Lr9, Lr19, Lr23, Lr24, Lr25, Lr29, Lr35, Lr37) exhibited little or no infection. Lines carrying genes Lr13, Lr44 and LrB were resistant in two years and those carrying Lr34, Lr38 and LrW in three years, after which they suffered moderate or heavy infection. Three lines (Lr12, Lr17, Lr32) proved to be moderately resistant. The majority of the wheat lines tested became heavily infected.

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Four Consistent Loci Confer Adult Plant Resistance to Leaf Rust in the Durum Wheat Lines Heller#1 and Dunkler

Phytopathology ◽

10.1094/phyto-09-19-0348-r ◽

2020 ◽

Vol 110 (4) ◽

pp. 892-899 ◽

Cited By ~ 1

Author(s):

Zhikang Li ◽

Chan Yuan ◽

Sybil A. Herrera-Foessel ◽

Mandeep S. Randhawa ◽

Julio Huerta-Espino ◽

...

Keyword(s):

Durum Wheat ◽

Leaf Rust ◽

Plant Resistance ◽

Rust Resistance ◽

Adult Plant Resistance ◽

Leaf Rust Resistance ◽

Adult Plant ◽

Susceptible Parent ◽

Phenotypic Data ◽

Wheat Lines

The durum wheat lines Heller#1 and Dunkler from the International Maize and Wheat Improvement Center Global Wheat Program showed moderate and stable adult plant resistance to leaf rust under high disease pressure over field environments in northwestern Mexico. Leaf rust phenotyping was performed on two recombinant inbred line (RIL) populations derived from crosses of Heller#1 and Dunkler with the susceptible parent Atred#2, conducted under artificially induced Puccinia triticina epidemics in 2013, 2014, 2015, and 2016. The Atred#2 × Heller#1 and Atred#2 × Dunkler populations were genotyped by single nucleotide polymorphism (SNP) platforms and diversity arrays technology markers, respectively. Four leaf rust resistance quantitative trait loci were detected simultaneously in the two RIL populations: Lr46, QLr.cim-2BC, QLr.cim-5BL, and QLr.cim-6BL based on phenotypic data across all four crop seasons. They explained 11.7 to 46.8%, 7.2 to 26.1%, 8.4 to 24.1%, and 12.4 to 28.5%, respectively, of the phenotypic variation for leaf rust resistance in Atred#2 × Heller#1 and 16.3 to 56.6%, 6.7 to 15.7%, 4.1 to 10.1%, and 5.1 to 20.2% of the variation in the Atred#2 × Dunkler population. Only the resistance allele of QLr.cim-2BC was from the susceptible parent Atred#2, and resistance alleles at other loci came from the resistant parents Heller#1 and Dunkler. The SNP markers closely linked to Lr46 and QLr.cim-2BC were converted to kompetitive allele specific PCR markers for use in marker-assisted selection to improve leaf rust resistance through crosses with Heller#1 and Dunkler sources.

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Inheritance of leaf rust resistance in wheat lines carryingAegilops speltoides Tausch. translocation in Chinese Spring background

Journal of Applied Genetics ◽

10.1007/bf03195607 ◽

2008 ◽

Vol 49 (2) ◽

pp. 141-145 ◽

Cited By ~ 3

Author(s):

Urmil K. Bansal ◽

Ram G. Saini ◽

Renu Khanna

Keyword(s):

Leaf Rust ◽

Chinese Spring ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Wheat Lines

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AGRONOMIC AND QUALITY CHARACTERISTICS OF WHEAT LINES WITH LEAF RUST RESISTANCE DERIVED FROM TRITICUM SPELTOIDES

Canadian Journal of Genetics and Cytology ◽

10.1139/g81-052 ◽

1981 ◽

Vol 23 (3) ◽

pp. 475-480 ◽

Cited By ~ 20

Author(s):

D. R. Knott ◽

J. Dvořák

Keyword(s):

Leaf Rust ◽

Rust Resistance ◽

Leaf Rust Resistance ◽

Triticum Aestivum L ◽

Quality Characteristics ◽

Puccinia Recondita ◽

Recurrent Parent ◽

Wheat Lines ◽

Speltoides Chromosome ◽

Deleterious Genes

Eleven lines of wheat (Triticum aestivum L.) carrying resistance to leaf rust (Puccinia recondita Rob. ex. Desm.) derived from five accessions of Triticum speltoides Tausch were grown in yield tests in 1977 and 1979. The grain was tested for quality characteristics in both years. Although the lines had been backcrossed four or five times to either Manitou or Neepawa, only four of the eleven showed any real promise of equalling their recurrent parent in agronomic and quality characteristics. Lines derived from the same accession of T. speltoides were surprisingly variable. The generally deleterious effects of the transferred chromatin are due either to genes linked to the genes for leaf rust resistance plus incomplete compensation by the speltoides chromosome segment for the aestivum segment it replaced, or to the effects of additional translocations that were not eliminated during backcrossing. A second cycle of homoeologous recombination is proposed as a way to eliminate some of the deleterious genes.

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