Functional divergence and catalytic properties of dehydroascorbate reductase family proteins from Populus tomentosa

2013 ◽  
Vol 40 (8) ◽  
pp. 5105-5114 ◽  
Author(s):  
Zhen-Xin Tang ◽  
Hai-Ling Yang
Keyword(s):  
Catalytic Properties ◽  
Functional Divergence ◽  
Populus Tomentosa ◽  
Dehydroascorbate Reductase

scholarly journals Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10741
Author(s):  
Nan Chao ◽  
Qi Qi ◽  
Shuang Li ◽  
Brent Ruan ◽  
Xiangning Jiang ◽  
...  
Keyword(s):  
Shikimic Acid ◽  
Lignin Content ◽  
Expression Profiles ◽  
Populus Trichocarpa ◽  
Functional Divergence ◽  
Lignin Biosynthesis ◽  
Populus Tomentosa ◽  
Motif Analysis ◽  
Monolignol Biosynthesis ◽  
Populus Tomentosa Carr

Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.

scholarly journals Molecular Properties and Functional Divergence of the Dehydroascorbate Reductase Gene Family in Lower and Higher Plants

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145038 ◽  
Author(s):  
Yuan-Jie Zhang ◽  
Wei Wang ◽  
Hai-Ling Yang ◽  
Yue Li ◽  
Xiang-Yang Kang ◽  
...  
Keyword(s):  
Gene Family ◽  
Higher Plants ◽  
Functional Divergence ◽  
Molecular Properties ◽  
Dehydroascorbate Reductase ◽  
Reductase Gene

Microstructure and reactivity of small metal particles: The role of Ce additives

1992 ◽  
Vol 50 (1) ◽  
pp. 318-319
Author(s):  
L.D. Schmidt ◽  
K. R. Krause ◽  
J. M. Schwartz ◽  
X. Chu
Keyword(s):  
Atmospheric Pressure ◽  
Noble Metals ◽  
Mixed Oxides ◽  
Catalytic Properties ◽  
Chemical Shifts ◽  
Catalytic Converter ◽  
Structural Evolution ◽  
Single Particles ◽  
Small Metal Particles

The evolution of microstructures of 10- to 100-Å diameter particles of Rh and Pt on SiO2 and Al2O3 following treatment in reducing, oxidizing, and reacting conditions have been characterized by TEM. We are able to transfer particles repeatedly between microscope and a reactor furnace so that the structural evolution of single particles can be examined following treatments in gases at atmospheric pressure. We are especially interested in the role of Ce additives on noble metals such as Pt and Rh. These systems are crucial in the automotive catalytic converter, and rare earths can significantly modify catalytic properties in many reactions. In particular, we are concerned with the oxidation state of Ce and its role in formation of mixed oxides with metals or with the support. For this we employ EELS in TEM, a technique uniquely suited to detect chemical shifts with ∼30Å resolution.

Electron distribution change of small nickel particles encaged in X zeolite in presence of Ce3+ ions, related to their catalytic properties

1986 ◽  
Vol 83 ◽  
pp. 619-621 ◽  
Author(s):  
F. Vergand ◽  
B. Iraqi ◽  
C. Bonnelle ◽  
E. Ramaroson ◽  
M.F. Guilleux ◽  
...  
Keyword(s):  
Electron Distribution ◽  
Catalytic Properties ◽  
Distribution Change ◽  
Nickel Particles ◽  
X Zeolite

On the Preparation of Bovine α-Thrombin

1978 ◽  
Vol 39 (01) ◽  
pp. 193-200 ◽  
Author(s):  
Erwin F Workman ◽  
Roger L Lundblad
Keyword(s):  
Immobilized Enzyme ◽  
Catalytic Properties ◽  
Specific Activity ◽  
Guanidine Hydrochloride ◽  
Low Molecular Weight ◽  
Reversible Process ◽  
Gel Beads ◽  
Tissue Thromboplastin ◽  
C 50 ◽  
Hydrolysis Of

SummaryAn improved method for the preparation of bovine α-thrombin is described. The procedure involves the activation of partially purified prothrombin with tissue thromboplastin followed by chromatography on Sulfopropyl-Sephadex C-50. The purified enzyme is homogeneous on polyacrylamide discontinuous gel electrophoresis and has a specific activity toward fibrinogen of 2,200–2,700 N.I.H. U/mg. Its stability on storage in liquid media is dependent on both ionic strenght and temperature. Increasing ionic strength and decreasing temperature result in optimal stability. The denaturation of α-thrombin by guanidine hydrochloride was found to be a partially reversible process with the renatured species possessing properties similar to “aged” thrombin. In addition, the catalytic properties of a-thrombin covalently attached to agarose gel beads were also examined. The activity of the immobilized enzyme toward fibrinogen was affected to a much greater extent than was the hydrolysis of low molecular weight, synthetic substrates.

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