Overexpression of Maize ZmHDZIV14 Increases Abscisic Acid Sensitivity and Mediates Drought and Salt Stress in Arabidopsis and Tobacco

2020 ◽  
Author(s):  
Peng Fang ◽  
Huiping Yan ◽  
Fenqi Chen ◽  
Yunling Peng
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Acid Sensitivity ◽  
Drought And Salt Stress

scholarly journals AtERF60 negatively regulates ABR1 to modulate salt, drought, and basal resistance in Arabidopsis

2021 ◽  
Author(s):  
Gajendra Singh Jeena ◽  
Ujjal Jyoti Phukan ◽  
Neeti Singh ◽  
Ashutosh Joshi ◽  
Alok Pandey ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Stress Responses ◽  
Biotic Stress ◽  
Bacterial Pathogen ◽  
Pcr Analysis ◽  
Cis Elements ◽  
Abiotic And Biotic Stress ◽  
Basal Resistance ◽  
Drought And Salt Stress

ABSCISIC ACID REPRESSOR-1 (ABR1), an APETALA2 (AP2) domain containing transcription factor (TF) contribute important function against variety of external cues. Here, we report an AP2/ERF TF, AtERF60 that serves as an important regulator of ABR1 gene. AtERF60 is induced in response to drought, salt, abscisic acid (ABA), salicylic acid (SA), and bacterial pathogen PstDC3000 infection. AtERF60 interacts with DEHYDRATION RESPONSE ELEMENTS (DRE1/2) and GCC box indicating its ability to regulate multiple responses. Overexpression of AtERF60 results in the drought and salt stress tolerant phenotype in both seedling and mature Arabidopsis plants in comparison with the wild type (WT-Col). However, mutation in AtERF60 showed hyperactive response against drought and salt stress in comparison with its overexpression and WT. Microarray and qRT-PCR analysis of overexpression and mutant lines indicated that AtERF60 regulates both abiotic and biotic stress inducible genes. One of the differentially expressing transcripts was ABR1 and we found that AtERF60 interacts with the DRE cis-elements present in the ABR1 promoter. The mutation in AtERF60 showed ABA hypersensitive response, increased ABA content, and reduced susceptibility to PstDC3000. Altogether, we conclude that AtERF60 represses ABR1 transcript by binding with the DRE cis-elements and modulates both abiotic and biotic stress responses in Arabidopsis.

scholarly journals SlbZIP38, a Tomato bZIP Family Gene Downregulated by Abscisic Acid, Is a Negative Regulator of Drought and Salt Stress Tolerance

Genes ◽  
2017 ◽  
Vol 8 (12) ◽  
pp. 402 ◽  
Author(s):  
Yanglu Pan ◽  
Xin Hu ◽  
Chunyan Li ◽  
Xing Xu ◽  
Chenggang Su ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Stress Tolerance ◽  
Negative Regulator ◽  
Salt Stress Tolerance ◽  
Drought And Salt Stress ◽  
Family Gene ◽  
Bzip Family

scholarly journals CaDHN4, a Salt and Cold Stress-Responsive Dehydrin Gene from Pepper Decreases Abscisic Acid Sensitivity in Arabidopsis

2019 ◽  
Vol 21 (1) ◽  
pp. 26
Author(s):  
Hua-feng Zhang ◽  
Su-ya Liu ◽  
Ji-hui Ma ◽  
Xin-ke Wang ◽  
Saeed ul Haq ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Cold Stress ◽  
Stress Responses ◽  
Electrolyte Leakage ◽  
Cellular Localization ◽  
Primary Root ◽  
Acid Sensitivity ◽  
Aba Sensitivity ◽  
Dehydrin Gene

Dehydrins play an important role in improving plant resistance to abiotic stresses. In this study, we isolated a dehydrin gene from pepper (Capsicum annuum L.) leaves, designated as CaDHN4. Sub-cellular localization of CaDHN4 was to be found in the nucleus and membrane. To authenticate the function of CaDHN4 in cold- and salt-stress responses and abscisic acid (ABA) sensitivity, we reduced the CaDHN4 expression using virus-induced gene silencing (VIGS), and overexpressed the CaDHN4 in Arabidopsis. We found that silencing of CaDHN4 reduced the growth of pepper seedlings and CaDHN4-silenced plants exhibited more serious wilting, higher electrolyte leakage, and more accumulation of ROS in the leaves compared to pTRV2:00 plants after cold stress, and lower chlorophyll contents and higher electrolyte leakage compared to pTRV2:00 plants under salt stress. However, CaDHN4-overexpressing Arabidopsis plants had higher seed germination rates and post-germination primary root growth, compared to WT plants under salt stress. In response to cold and salt stresses, the CaDHN4-overexpressed Arabidopsis exhibited lower MDA content, and lower relative electrolyte leakage compared to the WT plants. Under ABA treatments, the fresh weight and germination rates of transgenic plants were higher than WT plants. The transgenic Arabidopsis expressing a CaDHN4 promoter displayed a more intense GUS staining than the normal growth conditions under treatment with hormones including ABA, methyl jasmonate (MeJA), and salicylic acid (SA). Our results suggest that CaDHN4 can protect against cold and salt stresses and decrease ABA sensitivity in Arabidopsis.

scholarly journals Tartary Buckwheat Transcription Factor FtbZIP5, Regulated by FtSnRK2.6, Can Improve Salt/Drought Resistance in Transgenic Arabidopsis

2020 ◽  
Vol 21 (3) ◽  
pp. 1123 ◽  
Author(s):  
Qi Li ◽  
Haixia Zhao ◽  
Xiaoli Wang ◽  
Jingyue Kang ◽  
Bingbing Lv ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Salt Stress ◽  
Transgenic Plants ◽  
Transcriptional Activation ◽  
Transgenic Arabidopsis ◽  
Ectopic Expression ◽  
Tartary Buckwheat ◽  
Physiological Level ◽  
Expression Levels ◽  
Drought And Salt Stress

bZIP transcription factors have been reported to be involved in many different biological processes in plants. The ABA (abscisic acid)-dependent AREB/ABF-SnRK2 pathway has been shown to play a key role in the response to osmotic stress in model plants. In this study, a novel bZIP gene, FtbZIP5, was isolated from tartary buckwheat, and its role in the response to drought and salt stress was characterized by transgenic Arabidopsis. We found that FtbZIP5 has transcriptional activation activity, which is located in the nucleus and specifically binds to ABRE elements. It can be induced by exposure to PEG6000, salt and ABA in tartary buckwheat. The ectopic expression of FtbZIP5 reduced the sensitivity of transgenic plants to drought and high salt levels and reduced the oxidative damage in plants by regulating the antioxidant system at a physiological level. In addition, we found that, under drought and salt stress, the expression levels of several ABA-dependent stress response genes (RD29A, RD29B, RAB18, RD26, RD20 and COR15) in the transgenic plants increased significantly compared with their expression levels in the wild type plants. Ectopic expression of FtbZIP5 in Arabidopsis can partially complement the function of the ABA-insensitive mutant abi5-1 (abscisic acid-insensitive 5-1). Moreover, we screened FtSnRK2.6, which might phosphorylate FtbZIP5, in a yeast two-hybrid experiment. Taken together, these results suggest that FtbZIP5, as a positive regulator, mediates plant tolerance to salt and drought through ABA-dependent signaling pathways.

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