Androgenic haploid plant development via embryogenesis with simultaneous determination of bioactive metabolites in Cambod tea (Camellia assamica ssp. lasiocalyx)

2022 ◽  
Author(s):  
Vijay Kumar Mishra ◽  
Ruchira Bajpai ◽  
Rakhi Chaturvedi
Keyword(s):  
Simultaneous Determination ◽  
Plant Development ◽  
Bioactive Metabolites ◽  
Haploid Plant

scholarly journals Cyanobacterial Toxins and Peptides in Lake Vegoritis, Greece

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 394
Author(s):  
Sevasti-Kiriaki Zervou ◽  
Kimon Moschandreou ◽  
Aikaterina Paraskevopoulou ◽  
Christophoros Christophoridis ◽  
Elpida Grigoriadou ◽  
...  
Keyword(s):  
Public Health ◽  
Risk Assessment ◽  
Simultaneous Determination ◽  
Aquatic Ecosystems ◽  
Human Exposure ◽  
Bioactive Metabolites ◽  
Cyanobacterial Toxins ◽  
Major Threat ◽  
Almost All

Cyanotoxins (CTs) produced by cyanobacteria in surface freshwater are a major threat for public health and aquatic ecosystems. Cyanobacteria can also produce a wide variety of other understudied bioactive metabolites such as oligopeptides microginins (MGs), aeruginosins (AERs), aeruginosamides (AEGs) and anabaenopeptins (APs). This study reports on the co-occurrence of CTs and cyanopeptides (CPs) in Lake Vegoritis, Greece and presents their variant-specific profiles obtained during 3-years of monitoring (2018–2020). Fifteen CTs (cylindrospermopsin (CYN), anatoxin (ATX), nodularin (NOD), and 12 microcystins (MCs)) and ten CPs (3 APs, 4 MGs, 2 AERs and aeruginosamide (AEG A)) were targeted using an extended and validated LC-MS/MS protocol for the simultaneous determination of multi-class CTs and CPs. Results showed the presence of MCs (MC-LR, MC-RR, MC-YR, dmMC-LR, dmMC-RR, MC-HtyR, and MC-HilR) and CYN at concentrations of <1 μg/L, with MC-LR (79%) and CYN (71%) being the most frequently occurring. Anabaenopeptins B (AP B) and F (AP F) were detected in almost all samples and microginin T1 (MG T1) was the most abundant CP, reaching 47.0 μg/L. This is the first report of the co-occurrence of CTs and CPs in Lake Vegoritis, which is used for irrigation, fishing and recreational activities. The findings support the need for further investigations of the occurrence of CTs and the less studied cyanobacterial metabolites in lakes, to promote risk assessment with relevance to human exposure.

scholarly journals Androgenic Haploid Plant Development Via Embryogenesis With Simultaneous Determination of Bioactive Metabolites in Cambod Tea (Camellia Assamica ssp. Lasiocalyx)

2021 ◽  
Author(s):  
Vijay Kumar Mishra ◽  
Ruchira Bajpai ◽  
Rakhi Chaturvedi
Keyword(s):  
Plant Development ◽  
Epigallocatechin Gallate ◽  
Metabolite Profile ◽  
Hot Water ◽  
Bioactive Metabolites ◽  
Root Tip ◽  
Haploid Plant ◽  
Donor Plant ◽  
Breeding Lines

Abstract This pioneering work reports successful androgenic plant development via embryogenesis from microspore calluses in anther cultures and estimation of bioactive metabolites in in vitro regenerants and parent plant (control) of Cambod tea, Camellia assamica ssp. lasiocalyx (Planch MS) cultivar TV19. Anthers bearing microspores at early-to-late uni-nucleate stage were selected to initiate androgenesis. A pre-treatment of 50 C for five days in the dark was most effective to initiate profusely growing white callusing from microspores within 10 weeks of culture on MS medium (6% sucrose) supplemented with high cytokinins/ auxin ratio maintained by benzyl adenine (BAP) and 2,4-dichlorophenxyacetic acid (2, 4-D). Nodular structures on the callus surface differentiated into embryos. Further developement of the embryos occurred on embryogenesis medium but, with ten times reduced concentration of growth regulators and additives. Germination of embryos into complete plantlets was achieved when major salts in medium were reduced to half MS (½ MS) and augmented with BAP, GA3 and IBA along with glutamine and serine. Cytological examination of the root-tip cells revealed that regenerated plantlets were haploids (2n=x=15), which was further confirmed through flow cytometry. The hot-water extracts from in vitro haploid calluses, embryos and field-grown donor plant were utilized for quantification of (+)-catechin, (-)-epicatechin, (-)-epigallocatechin gallate, caffeine and theophylline. Our findings revealed that the metabolite profile of in vitro regenerated haploid cultures is comparable to that of the mother plant, thereby presenting them as potential source for genome duplication and development of genetically stable homozygous pure breeding lines.

A general computer program for the extended plate overlap algorithm

1978 ◽  
Vol 48 ◽  
pp. 287-293 ◽  
Author(s):  
Chr. de Vegt ◽  
E. Ebner ◽  
K. von der Heide
Keyword(s):  
Computer Program ◽  
Simultaneous Determination ◽  
General Computer Program ◽  
General Computer

In contrast to the adjustment of single plates a block adjustment is a simultaneous determination of all unknowns associated with many overlapping plates (star positions and plate constants etc. ) by one large adjustment. This plate overlap technique was introduced by Eichhorn and reviewed by Googe et. al. The author now has developed a set of computer programmes which allows the adjustment of any set of contemporaneous overlapping plates. There is in principle no limit for the number of plates, the number of stars, the number of individual plate constants for each plate, and for the overlapping factor.

The Simultaneous Determination of Plant and Leased Warehouse Capacities for a Firm Facing Uncertain Demand in Several Regions

1982 ◽  
Vol 14 (2) ◽  
pp. 99-108 ◽  
Author(s):  
James V. Jucker ◽  
Robert C. Carlson ◽  
Dean H. Kropp
Keyword(s):  
Simultaneous Determination ◽  
Uncertain Demand

Simultaneous determination of seven compounds in Euronymus alatus by HPLC-DAD

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
YS Jung ◽  
JB Weon ◽  
CJ Ma
Keyword(s):  
Simultaneous Determination

Simultaneous determination of monomeric and oligomeric alkannin and shikonin by HPLC-DAD-APCI-MS

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
AN Assimopoulou ◽  
M Ganzera ◽  
H Stuppner ◽  
VP Papageorgiou
Keyword(s):  
Simultaneous Determination
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