Partial alleviation of zinc induced oxidative stress by polyamines in Plantago ovata Forsk

Responses to Salinity in Four Plantago Species from Tunisia

Plants ◽

10.3390/plants10071392 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1392

Author(s):

Hela Belhaj Ltaeif ◽

Anis Sakhraoui ◽

Sara González-Orenga ◽

Anbu Landa Faz ◽

Monica Boscaiu ◽

...

Keyword(s):

Oxidative Stress ◽

Salt Stress ◽

Stress Responses ◽

High Salinity ◽

Plantago Ovata ◽

Stress Markers ◽

As Species ◽

Nacl Concentration ◽

Antioxidant Metabolites ◽

Mechanisms Of Salt Tolerance

The genus Plantago is particularly interesting for studying the mechanisms of salt tolerance in plants, as it includes both halophytes and glycophytes, as well as species adapted to xeric environments. In this study, the salt stress responses of two halophytes, P. crassifolia and P. coronopus, were compared with those of two glycophytes, P. ovata and P. afra. Plants obtained by seed germination of the four species, collected in different regions of Tunisia, were subjected to increasing salinity treatments for one month under greenhouse conditions. Morphological traits and biochemical parameters, such as ion accumulation and the leaf contents of photosynthetic pigments, osmolytes, oxidative stress markers and antioxidant metabolites, were measured after the treatments. Salt-induced growth inhibition was more pronounced in P. afra, and only plants subjected to the lowest applied NaCl concentration (200 mM) survived until the end of the treatments. The biochemical responses were different in the two groups of plants; the halophytes accumulated higher Na+ and proline concentrations, whereas MDA levels in their leaves decreased, indicating a lower level of oxidative stress. Overall, the results showed that P. coronopus and P. crassifolia are the most tolerant to salt stress, and P. afra is the most susceptible of the four species. Plantago ovata is also quite resistant, apparently by using specific mechanisms of tolerance that are more efficient than in the halophytes, such as a less pronounced inhibition of photosynthesis, the accumulation of higher levels of Cl− ions in the leaves, or the activation of K+ uptake and transport to the aerial part under high salinity conditions.

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Alleviation of zinc induced oxidative stress by polyamines in Plantago ovata Forsk

10.21203/rs.3.rs-335842/v1 ◽

2021 ◽

Author(s):

Paulami Pramanick ◽

Anindita Chakraborty ◽

Sarmistha Sen Raychaudhuri

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Heavy Metal ◽

Chlorophyll Content ◽

Quantitative Methods ◽

Antioxidant Properties ◽

Membrane Integrity ◽

Antioxidant Systems ◽

Plantago Ovata ◽

Induced Stress

Abstract Zinc causes toxicity to the plants in an excess concentration and it is manifested by chlorosis, rolling of leaf margins, and disruption of membrane integrity. The heavy metal stress also triggers the stimulation of enzymatic and non-enzymatic antioxidant systems. Polyamines are naturally occurring, secondary metabolites, protecting plants from heavy metal-induced stress. Plants also up-regulate the mRNA expression of Metallothionein in response to heavy metal-induced oxidative stress. The alteration in Metallothionein type 2 (PoMT2) expression of a medicinally important herb Plantago ovata in presence of polyamines like Putrescine, Spermidine, and Spermine in addition to ZnSO4.H2O by the semi-quantitative and the quantitative methods have been demonstrated in the present study. We have observed reductions in the expression of the Metallothionein type 2 gene in the presence of the aforementioned polyamines which implies their protective and antioxidant properties to fight against the zinc induced stress. 1 mM Put has been more efficient in increasing the total chlorophyll content (compared to 2 mM Put) by about 36% each in 1000 µM ZnSO4 treated P. ovata seedlings. Spermidine also enhanced chlorophyll content. 2 mM Put and 0.5 mM Spm have shown even better efficiencies in increasing the total antioxidant and DPPH radical scavenging activities. The lipid peroxidation has been found to decrease in Put and Spm supplemented samples by up to about 47% in both cases. Significant reductions in lipid peroxidation and down-regulation of PoMT2 gene expression indicate the roles of polyamines in partially alleviating Zn-induced oxidative damage.

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Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

Food & Function ◽

10.1039/d0fo01638a ◽

2020 ◽

Vol 11 (10) ◽

pp. 8547-8559

Author(s):

Hongjing Zhao ◽

Yu Wang ◽

Mengyao Mu ◽

Menghao Guo ◽

Hongxian Yu ◽

...

Keyword(s):

Oxidative Stress ◽

Secondary Metabolites ◽

Human Health ◽

Grass Carp ◽

Aquatic Environment ◽

Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

Biochemical Journal ◽

10.1042/bcj20190591 ◽

2019 ◽

Vol 476 (24) ◽

pp. 3705-3719 ◽

Cited By ~ 2

Author(s):

Avani Vyas ◽

Umamaheswar Duvvuri ◽

Kirill Kiselyov

Keyword(s):

Oxidative Stress ◽

Head And Neck ◽

Transcriptional Activation ◽

Platinum Resistance ◽

Mrna Levels ◽

Strong Positive Correlation ◽

Platinum Compounds ◽

Copper Chelation ◽

Novel Approach ◽

Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

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Clinical aspects of reactive oxygen and nitrogen species

Biochemical Society Symposium ◽

10.1042/bss0710121 ◽

2004 ◽

Vol 71 ◽

pp. 121-133 ◽

Cited By ~ 25

Author(s):

Ascan Warnholtz ◽

Maria Wendt ◽

Michael August ◽

Thomas Münzel

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Risk Factor ◽

Endothelial Dysfunction ◽

Vascular Tissue ◽

Rapid Development ◽

Reactive Oxygen ◽

Clinical Aspects ◽

No Production ◽

Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

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