scholarly journals Efficient plant regeneration via meristematic nodule culture in Paeonia ostii ‘Feng Dan’

2022 ◽  
Author(s):  
Li Xu ◽  
Fangyun Cheng ◽  
Yuan Zhong
Keyword(s):  
Industrial Development ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Attractive Alternative ◽  
Tree Peony ◽  
Induction Rate ◽  
Meristematic Nodules ◽  
Propagation Methods

AbstractTree peony (Paeonia sect. Moutan) is an economically important multipurpose woody plant in terms of its medical, ornamental and oil values, but its breeding and industrial development are severely limited due to inefficient traditional propagation methods and existing in vitro regeneration systems. Meristematic nodules (MNs) are an attractive alternative to solve this problem. This study first presented a protocol for in vitro regeneration of P. ostii ‘Feng Dan’ via MN culture with four consecutive steps, including embryogenic callus (EC) formation, MN induction and leaf cluster differentiation, shoot elongation, rooting and acclimatization. The highest EC induction rate (81.25%) was achieved when cotyledons were cultured on modified Murashige and Skoog (mMS) medium with 4.04 µM N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) + 5.37 µM α-naphthylacetic acid (NAA) for 30 days. The optimal MN induction rate (100%) and leaf cluster differentiation rate (45.83%) were obtained when ECs were cultured on modified woody plant medium (mWPM) supplemented with 2.02 µM CPPU + 2.27 µM thidiazuron (TDZ) for a subculture time of 10 days. The combination of 1.29 µM 6-benzyladenine (BA) + 0.58 µM gibberellin (GA3) yielded the best shoot elongation (13.40 shoots per nodule), rooting rate (43.33%) and consequently survival rate (45.83%). The study will be beneficial to the mass propagation, breeding and genetic improvement of tree peony.

scholarly journals Essential factors for in vitro regeneration of rose and a protocol for plant regeneration from leaves 

2018 ◽  
Vol 45 (No. 2) ◽  
pp. 83-91
Author(s):  
Anber Mahmoud Ahmed Hassanein ◽  
Inas Mohamed Ali Mahmoud
Keyword(s):  
Indole Acetic Acid ◽  
Culture Medium ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Favorable Effect ◽  
Indole Butyric Acid ◽  
Leaf Discs ◽  
Better Than

In vitro propagation of Rosa hybrida, L. cv. ‘Eiffel Tower’ was improved by the addition of thidiazuron (TDZ) and silver nitrate (AgNo<sub>3</sub>) to the culture medium. The combination of auxin and cytokinins was indispensable for inducing response from leaf discs. Maintaining cultures under dark was better than light for callus formation and quality. The source of explants was vital in the regeneration process wherein situ explants produced callus while, in vitro explants regenerated somatic embryos and shoots. Gibberellic acid (GA<sub>3</sub>) had a favorable effect where in vitro explants showed somatic embryogenesis with no shoots on media containing TDZ however, 37% of explants regenerated shoots directly on medium containing GA<sub>3</sub>. The presence of benzyl adenine (BA) was essential for shoot elongation, and indole butyric acid (IBA) was better than indole acetic acid (IAA) for rooting. The optimum conditions produced rooted plants from leaf discs within ten weeks. The reported results clarify factors controlling in vitro regeneration of R. hybrida, and provide a rapid protocol allowing further improvements of rose. 

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals Pre-forcing Treatments Influence Bud Break and Shoot Elongation in Forced Woody Species

1992 ◽  
Vol 10 (2) ◽  
pp. 101-103
Author(s):  
Guochen Yang ◽  
Paul E. Read
Keyword(s):  
In Vitro Culture ◽  
Plant Species ◽  
Woody Plants ◽  
Woody Plant ◽  
Woody Species ◽  
Extended Period ◽  
Shoot Elongation ◽  
Bud Break ◽  
Cold Requirement

Abstract Experiments were conducted to evaluate the feasibility of pre-forcing treatments for the release of bud dormancy of dormant stems of lilac, privet and Vanhoutte spirea. The new softwood growth of these dormant stems was used either as explants for in vitro culture or as cuttings for rooting studies of woody plant species in the off-season. A pre-forcing 15% bleach solution (0.78% NaOCl) soak hastened bud break, enhanced percentage of bud break, and promoted shoot elongation. Pre-forcing wetting agent treatments produced similar results to those of the bleach soak with variation among wetting agents and plant species. Smaller treatment differences were observed in the forcing characteristics when stems were collected later in the winter, probably because the cold requirement of the buds had been completely or partially met. This technique will provide explants for in vitro culture and softwood cuttings for propagation of woody plants over an extended period.

scholarly journals In-vitro Callus Induction and Regeneration of Brinjal (Solanum melongena L.) through Cotyledon

2020 ◽  
Author(s):  
Shreedhar Ganapati Bhat ◽  
G. Arulananthu ◽  
N. Ramesh
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Solanum Melongena ◽  
Shoot Elongation ◽  
Vegetable Crops ◽  
Plant Growth Hormones ◽  
Culture Techniques ◽  
Cotyledon Explants

Brinjal is one of the most popular, nutritional and vegetable crops in the world. It plays a vital role in the national economy as a cash crop. Tissue culture techniques used for in-vitro plant regeneration through cotyledon explants of eggplant (Solanum melongena L.) with different combinations of plant growth hormones BAP (4.44, 6.66, 8.88, 11.10 and 13.32µM) and IAA (0.57, 1.14 and 1.71µM) used for in-vitro regeneration of brinjal. The cotyledon explants used in this study, the highest callus induction found on BAP 8.88 µM and IAA 1.14 µM. The callus induction occurred after 15days from initiation, shoot induction occurred after 30 days from initiation and shoot elongation was carried out on the same medium, shoot elongation occurred after 45 days from initiation. MS hormone-free medium found best for root regeneration, the elongated shoots were selected and transferred to a test tube containing MS hormone-free rooting medium and the elongated shoots produce roots after 15 days. Then the rooted plantlets were transferred to poly-cup with a pre-sterilized mixture of coco peat for primary hardening under poly-tunnel for 10days. Subsequently, there generated plantlets acclimatized under the greenhouse. Then, hardened plants transferred to the open field for further development. This plant regeneration method can be useful for the production of the disease-free plant.

scholarly journals (175) In Vitro Regeneration of Cladrastis kentukea

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052C-1052
Author(s):  
Denita Hadziabdic ◽  
Robert N. Trigiano ◽  
Stephen Garton ◽  
Mark T. Windham ◽  
William E. Klingeman
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Cambial Activity ◽  
Ms Medium ◽  
Total Root Length ◽  
Woody Plant Medium ◽  
Half Strength

Axillary buds from a single Cladrastis kentukea tree were initially cultured on two media, woody plant medium (WPM) and Murashige and Skoog (MS) containing 0, 1, 2, or 4 μm 6–benzylaminopurine (BA). Cultures were transferred to fresh media every 4 weeks. Elongated shoots were harvested after 39 weeks and transferred to half-strength MS medium supplemented with the following concentrations of IBA: 0, 3, 30, 100, and 300 μm for 3 d, then returned to half-strength MS without growth regulators. Explants exposed to 300 μm of IBA produced significantly more roots (75%) compared to explants exposed to other treatments. Fifty-four and 45% of the microshoots rooted when exposed to 100 and 30 μm IBA, respectively. Only 4% of the microshoots rooted when exposed to 3 μm IBA and none of the control microshoots rooted. Although the 300 μm treatment yielded the most rooted plantlets, there was significantly higher terminal meristem abortion compared to other treatments. There were no statistical differences between the numbers of roots and total root length among all treatments. Additionally, all microshoots that rooted had lenticels, suggesting that presence of lenticel cambial activity can possibly improve rooting abilities of selected microshoots. Rooted microshoots were gradually acclimatized to nonsterile environment.

scholarly journals (174) In Vitro Regeneration of Cornus kousa

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1052B-1052
Author(s):  
Denita Hadziabdic ◽  
Robert N. Trigiano ◽  
Stephen Garton ◽  
Mark T. Windham
Keyword(s):  
Woody Plant ◽  
In Vitro Regeneration ◽  
Root Production ◽  
Naphthaleneacetic Acid ◽  
Apical Buds ◽  
Cornus Kousa ◽  
Woody Plant Medium ◽  
Rooting Media ◽  
Indole 3 Acetic Acid

Axillary and apical buds from five Cornus kousa cultivars (`Little Beauty', `Samaritan', `Heart Throb', `Rosabella', and `Christian Prince') were initially established on two basal media, woody plant medium (WPM) and woody plant medium/broad leaved tree medium (BW), amended with the following concentrations of 6–benzylaminopurine (BA): 0, 2, 4, and 8 μm. After explants were transferred at 4-week intervals for 28 weeks beginning in April, only microshoots of `Samaritan', `HeartThrob' and `Rosabella', were harvested from proliferating cultures and placed on rooting media. `Little Beauty' and `Christian Prince' did not perform well in multiplication phase of tissue culture and were excluded from further studies. Rooting media contained WPM or BW supplemented with either 1-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) at the following concentrations: 0, 0.5, 1.5, 4.5, and 13.5 μm. Six weeks following rooting experiment, preliminary data were collected and results showed that total of nine plants rooted on both WPM and BW media supplemented with IBA, 17 plants rooted on media supplemented with NAA, and 14 plants rooted on media supplemented with IAA. These results indicated that NAA and IAA appeared to be better for root production on C. kousa cultivar microshoots than IBA. Additionally, WPM supported more root production, when compared to BW, even though both media resulted in rooted microshoots. Proliferating masses were placed on fresh medium with 2μm BA and were used again for the rooting projects.

scholarly journals In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

2022 ◽  
Vol 31 (2) ◽  
pp. 143-151
Author(s):  
- Kamdem ◽  
Nehemie Tchinda Donfagsiteli ◽  
Njoueretou Mfondi Mache ◽  
Carine Temegne Nono ◽  
Rodrigue Goimasse ◽  
...  
Keyword(s):  
Culture Media ◽  
Zygotic Embryo ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Black Soil ◽  
Control Medium ◽  
Embryo Germination ◽  
Leaf Formation ◽  
Triton X

Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80 ± 4% disinfection rate with combination of triton x- 100 (0.2%) and sodium hypochlorite (30%). Embryo germination was highest on control medium. Rooting was higher (2±1 roots per embryo) after 4 weeks on control medium and on BAP supplemented medium at 0.8 μM while the longest root (1.5±0.5 cm) was observed on 2,4-D supplemented medium at 1.8 μM. Black soil was suitable for leaf formation (4 ± 2 leaves) and shoot elongation (2±1 cm) after 8 weeks in acclimatisation. These results show efficient disinfection, regeneration and acclimatisation of Picralima nitida. Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)

In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

2017 ◽  
Author(s):  
Shikha Sharma ◽  
Geetika Gambhir ◽  
D. K. Srivastava
Keyword(s):  
Pisum Sativum ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Cotyledonary Node ◽  
Shoot Elongation ◽  
Root Regeneration ◽  
Pisum Sativum L ◽  
Regenerated Plantlets

In vitro regeneration of pea explants (Pisum sativum L. var. ‘Lincon’) was done in 49 different combinations and concentrations of BAP, BAP and NAA, BAP and IBA, TDZ, TDZ and Adenine for shoot regeneration from hypocotyl, root, leaf and cotyledonary node. High frequency shoot regeneration was obtained in hypocotyl (81.43%), root(83.53%) and cotyledonary node(72.76%) on MS medium supplemented with 4.50 mg/l BAP and 1.86mg/l NAA, 2.00mg/l TDZ and4.50 mg/l BAP and 1.86mg/l NAA respectively. No shoot regeneration was obtained from leaf explants on any of the combination used. Shoot elongation was observed on the same medium used for shoot regeneration respectively.MS medium supplemented with 0.20 mg/l IBA was found best for root regeneration from in vitro raised shoots. The plantlets were able to regenerate within 6-7 weeks. The regenerated plantlets were acclimatized in pre-sterilized cocopeat.

scholarly journals In vitro propagation of Andrographis paniculata Nees.-A threatened medicinal plant of Bangladesh

2016 ◽  
Vol 3 (1) ◽  
pp. 67-73
Author(s):  
PK Roy
Keyword(s):  
Activated Charcoal ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Shoot Tip ◽  
Andrographis Paniculata ◽  
Nodal Segment ◽  
Threatened Medicinal Plant ◽  
Half Strength ◽  
Number Of Shoots

An efficient protocol was developed for in vitro regeneration of plantlets from shoot tip and nodal segment explants of Andrographis paniculata Nees. Nodal segment explants produced the highest number of shoots (18±1.24) when they were cultured on MS supplemented with 11.10 ?M/l BAP. Addition of 10% coconut water and 2.0 g/l activated charcoal to the above mentioned medium increased the number of shoots (30) per culture. Shoot tip explant also showed better performance in the same medium. Addition of 100 mg/l urea and 2.0 g/l activated charcoal to the medium showed proper shoot elongation. The isolated shoots rooted well (90%) on half-strength MS fortified with 9.80 ?M/l IBA, where average number of roots per shoot was 28-30. The plantlets were acclimatized successfully in poly bags containing a mixture of soil, sand and compost in 2:1:1 ratio. Finally acclimatized plantlets were transferred to experimental field.Jahangirnagar University J. Biol. Sci. 3(1): 67-73, 2014 (June)

scholarly journals Micropropagation of Shorea robusta: an economically important woody plant

2014 ◽  
Vol 60 (No. 2) ◽  
pp. 70-74 ◽  
Author(s):  
M. Singh ◽  
S. Sonkusale ◽  
Ch. Niratker ◽  
P. Shukla
Keyword(s):  
Tree Species ◽  
Woody Plants ◽  
Woody Plant ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shorea Robusta ◽  
Shoot Initiation

Shorea robusta is a valuable tree species which provides good quality timber along with other useful materials like seeds which can be used as a source of starch. Woody plants are difficult to regenerate under in vitro conditions and only some success has been achieved so far. Here we have presented the data for successful in vitro regeneration of S. robusta using nodal explants. Shoot proliferation and rooting were also successfully achieved in subsequent subcultures. The best medium for shoot initiation and proliferation was found to be WPM with 1.0 mg&middot;l<sup>&ndash;1</sup> BAP and 0.5 mg&middot;l<sup>&ndash;1</sup> NAA and 1.0 mg&middot;l<sup>&ndash;1</sup> BAP +0.5 mg&middot;l<sup>&ndash;1</sup> NAA, respectively. Likewise for rooting WPM medium with 0.5 mg&middot;l<sup>&ndash;1</sup> IBA was found to be the best medium. &nbsp; &nbsp;

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