Immunohistochemical expression of dogTERT in canine testicular tumours in relation to PCNA, ki67 and p53 expression.

2009 ◽  
Vol 33 (8) ◽  
pp. 905-919 ◽  
Author(s):  
N. Papaioannou ◽  
D. Psalla ◽  
M. Zavlaris ◽  
P. Loukopoulos ◽  
N. Tziris ◽  
...  
Keyword(s):  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Testicular Tumours

Aberrant p53 Expression in Feline Vaccine-associated Sarcomas and Correlation with Prognosis

2005 ◽  
Vol 42 (6) ◽  
pp. 805-811 ◽  
Author(s):  
A. E. Hershey ◽  
R. R. Dubielzig ◽  
M. L. Padilla ◽  
S. C. Helfand
Keyword(s):  
Suppressor Gene ◽  
Mutant P53 ◽  
Immunohistochemical Expression ◽  
Cytoplasmic Staining ◽  
P53 Expression ◽  
Significant Difference ◽  
Nonspecific Staining ◽  
Rabbit Polyclonal Antibody ◽  
Therapeutic Opportunity ◽  
Positive Immunoreactivity

Eighty spontaneously occurring feline vaccine-associated sarcomas (VAS) were evaluated to determine the immunohistochemical expression of the tumor suppressor gene p53. Sixty-five of 80 VAS (81%) exhibited positive immunoreactivity with Mab240, a murine monoclonal antibody that specifically recognizes mutated p53. Only 44 of 81 tumors (55%) were positive with rabbit polyclonal antibody CM-1. CM-1 often yielded nonspecific staining of nonneoplastic tissues. Nonspecific staining was greatly reduced or absent with Mab240. Cytoplasmic staining for p53 was a consistent pattern of VAS, occurring in 44% of tumors evaluated. Cats with tumors that exhibited cytoplasmic p53 had significantly shorter time to tumor recurrence compared to those cats with tumors that exhibited nuclear p53 staining ( P = 0.0284), but no significant difference in survival outcome was observed. Immunohistochemical detection of p53 offers a prognostic tool for VAS, and, because abnormal p53 expression appears to be a common feature of feline VAS, molecular targeting of mutant p53, may offer a promising new therapeutic opportunity for this cancer.

scholarly journals Immunohistochemical Expression of p53 and Bcl-2 in Algerian Cervical Carcinoma

2018 ◽  
Vol 11 (1) ◽  
pp. 67-75
Author(s):  
Deffar Khalissa ◽  
Khenchouche Abdelhalim ◽  
Xing Xie ◽  
Ying Li ◽  
Ouhida Soraya ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Prognostic Marker ◽  
Uterine Cervix ◽  
Prognostic Markers ◽  
P53 Protein ◽  
Immunohistochemical Expression ◽  
Important Indicator ◽  
P53 Expression

The Objective of the present study is to evaluate the expression levels of Bcl-2 and p53 proteins in squamous cell carcinoma and adenocarcinoma of the uterine cervix, and try to explain their role as prognostic markers for this cancer. The cohort comprised 90 cases of the cervix lesions. The samples were assessed by immunohistochemistry for the expression of Bcl-2 and p53 proteins. The results showed that the Bcl-2 expression was either absent, low or moderate respectively in 38.96%; 50.65% and 10.39% of SCC cases. However, it was absent or expressed in 76.92% and 23.08% of adenocarcinoma cases respectively. The p53 protein was absent or present respectively in 75.32% and 24.68% of SCC cases as demonstrated by immunohistochemistry. p53 was almost absent in adenocarcinoma samples where only 7.70% of cases were positive. There was no significant correlation between Bcl-2 and p53 expression (p=0.352). We conclude that p53 expression, detected by immunohistochemistry, does not appear to be a prognostic marker for cervical cancer. Nevertheless, Bcl-2 expression seems to provide more information for this disease. It may represent an important indicator for cervical cancer.

Endometrioid Endometrial Carcinoma With Spindle Cells–Aberrant p16 and p53 Expression

2018 ◽  
Vol 27 (2) ◽  
pp. 203-207
Author(s):  
Snehal Sonawane ◽  
Osama Elfituri ◽  
Yanmin Zhang ◽  
Edgardo Yordan ◽  
Nicholas Ree
Keyword(s):  
Endometrial Carcinoma ◽  
Spindle Cell ◽  
Tumor Type ◽  
Immunohistochemical Expression ◽  
Cell Component ◽  
Diagnostic Dilemma ◽  
P53 Overexpression ◽  
P53 Expression ◽  
Spindle Cells ◽  
Endometrioid Endometrial Carcinoma

Endometrioid carcinoma is known for its diverse morphology and may pose a diagnostic dilemma when it presents with a spindle cell component. We present a case of a 65-year-old woman with postmenopausal bleeding. Physical examination showed a mass protruding from the external cervical os. The patient underwent biopsy followed by hysterectomy. Pathologic examination showed an endometrioid endometrial carcinoma with spindle cell differentiation arising in an endometrial polyp, which raised a variety of differential diagnoses. Prior reports of this tumor type showed nonaberrant immunohistochemical expression of p16 and p53. However, this case showed p16 and p53 overexpression indicating that there is a spectrum of these tumors.

scholarly journals Immunohistochemical expression of P53 in basal cell carcinoma of skin.

2019 ◽  
Vol 26 (09) ◽  
pp. 1500-1505
Author(s):  
Saira Javeed ◽  
Ashok Kumar ◽  
Iram Asrar ◽  
Rabiya Fawad ◽  
Marrium Waqar ◽  
...  
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Sampling Technique ◽  
Cross Sectional Study ◽  
Immunohistochemical Expression ◽  
Cross Sectional ◽  
P53 Expression ◽  
Embedded Blocks ◽  
P53 Immunoreactivity

Objectives: To evaluate the immunohistochemical expression of p53 in basal cell carcinoma of skin. Study Design: It was cross sectional study. Setting: Pathology department of Pakistan Institute of Medical Sciences Hospital, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad, Pakistan. Period: Six months after approval from the Hospital Ethical Committee. Material and Methods: In a descriptive background, 50 cases were involved in the study. Cases were selected by non-probability consecutive sampling technique. Patients of all age group (Males and Females) that was diagnosed as basal cell carcinoma of skin by Hematoxylin & Eosin were included in study. Other epithelial tumors of skin, appendageal tumors and metastatic tumors were excluded. Cases were evaluated for expression of tumor suppressor protein-p53 by immunohistochemical technique applied on formalin-fixed paraffin-embedded blocks. Results: Out of 50 cases, majority of patients were found to be male. Ratio of male to female was 2.6:1. Age range of patient was found between 21-98 years. Mainstream of the patients were between 41-60 years. Nose was found to be frequently involved site 28 (56%) cases. p53 expression was seen in 42 (84%) cases while in 8 (16%) cases p53 expression was not seen. Conclusion: It was found that p53 expression rate is very high in basal cell carcinoma of skin. This high expression of p53 immunoreactivity was explained in terms of its pathogenetic role and mutation in basal cell carcinoma.

scholarly journals Interplay Between the Immunohistochemical Expression of P53 and the Proliferation Index in the Keratinocyte Tumors of the Skin

2018 ◽  
Vol 19 (4) ◽  
pp. 331-341
Author(s):  
Milena Rakocevic ◽  
Biljana Popovska Jovicic ◽  
Tomislav Jocic ◽  
Stevan Matic ◽  
Goran Azanjac ◽  
...  
Keyword(s):  
Cell Cycle Regulation ◽  
Significant Positive Correlation ◽  
Malignant Tumors ◽  
High Index ◽  
Proliferation Index ◽  
Tumor Localization ◽  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Positive Correlation ◽  
Cycle Regulation

Abstract P53 is important for cell cycle regulation, and its overexpression is seen in malignant tumors. We examined correlation between p53 expression and cell proliferation, and its role in the pathogenesis of keratinocyte skin tumors. We used biopsies from patients with squamous cell carcinoma, actinic keratosis and keratoacanthoma. We examined crosssections stained with HE and using anti-cytokeratin, antip53 and anti-Ki67 antibodies. Expression of p53 is found in 87, 85% of SCC, in 83. 3% of AK and 13. 4% KA. The high index of p53 expression was higher in SCC and AK compared to KA. We also observed a positive correlation between the expression of p53 and localization of the tumors. The largest proportion of subjects with AK and SCC has a high index of p53 expression on photoexposed region. We also observed that p53 expression correlates with age whereby in AK p53 expression increases with age. The high index of proliferation is most frequent in SCC and KA. Also at AK we found a strong correlation between a moderate proliferation index and tumor localization in photoexposed region. Between the proliferation index and p53 expression we observed a significant positive correlation only in SCC. Proliferation index and the expression of p53 are useful for the differentiation of precursor keratinocyte lesions and skin carcinoma. High p53 expression has been associated with the aging and significantly correlates with the exposure to UV radiation in SCC and AK. High expression of p53 in AK and SCC supports the importance of this oncoprotein in carcinogenesis of the skin.

scholarly journals IMMUNOHISTOCHEMICAL EXPRESSION OF P53 IN INVASIVE DUCTAL CARCINOMA OF BREAST IN PAKISTANI WOMEN

2021 ◽  
Vol 71 (Suppl-1) ◽  
pp. S142-46
Author(s):  
Umair Aslam Shahzad Khan ◽  
Muhammad Tahir Khadim ◽  
Syed Salman Ali ◽  
Muhammad Imran Khan Wajid ◽  
Akhter Ali Bajwa ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Invasive Ductal Carcinoma ◽  
Ductal Carcinoma ◽  
Histopathological Examination ◽  
Cross Sectional Study ◽  
Military Hospital ◽  
Immunohistochemical Expression ◽  
Cross Sectional ◽  
P53 Expression ◽  
Pakistani Women

Objective: To determine the frequency of immunohistochemical expression of p53 in invasive ductal carcinoma of breast in Pakistani women. Study Design: Descriptive, cross-sectional study. Place and Duration of Study: Department of Histopathology, Combined Military Hospital, Lahore, from Jul 2015 to Jan 2016. Methodology: This study involved 80 histopathological samples of invasive ductal carcinoma breast which weretested for p53 expression. Sample size with 80 cases was calculated using 95% confidence level and 10% marginof error while expected percentage of p53 expression will be 71.67%. Specimens were selected by non-probability, consecutive sampling. All specimens of newly diagnosed cases of invasive ducal carcinoma at CMH Lahore on histopathological examination of female patients aged between 15-80 years. IHC application of p53 was done as per technique mentioned in the manual given by the DAKO. Results: Patient age was calculated which was between 25 years and 80 years while mean age was 50.76 ± 11.80years. Majority of the patients were aged between 25-50 years (n=44, 55.0%). In 54 (67.5%) patients lymph nodes were positive for tumor metastasis. Majority 64 (80%) of the tumors were Grade-II followed by Grade-III in 15 (18.7%) patients. The size of the tumor ranged from 0.2 cm to 14 cm with a mean of 4.16 ± 2.59 cm. p53 expression was positive in 38 (47.5%) patients. The expression of p53 was higher in patients who have positive lymph nodes (59.3% vs. 23.1%; p=0.002). However, expression of p53 was not so significant for age (p=0.079), tumor size (p=0.414)......................

Immunohistochemical Expression of p63 and ΔNp63 in Mixed Tumors of Canine Mammary Glands and Its Relation with p53 Expression

2009 ◽  
Vol 46 (3) ◽  
pp. 407-415 ◽  
Author(s):  
A. C. Bertagnolli ◽  
G. D. Cassali ◽  
M. C. L. S. Genelhu ◽  
F. A. Costa ◽  
J. F. C. Oliveira ◽  
...  
Keyword(s):  
Malignant Tumors ◽  
Mammary Glands ◽  
Myoepithelial Cells ◽  
The Other ◽  
Benign Tumors ◽  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Positive Correlation ◽  
Mixed Tumors

The immunohistochemical expression of p63, ΔNp63, and p53 was studied in mixed tumors of canine mammary glands (13 benign mixed tumors and 19 carcinomas arising from benign mixed tumors) to determine the role of p63 and its isoform ΔNp63 in the development of mixed tumors, as well as to assess its relation with p53. P63 was expressed in myoepithelial cells of all benign mixed tumors and in 18 of 19 carcinomas in mixed tumors. The p63-negative carcinoma in mixed tumors was invasive, and a loss of p63 was detected in the other malignant tumors showing a discontinuous p63-stained myoepithelial layer. ΔNp63 was expressed in all benign mixed tumors but only in p63-positive carcinomas in mixed tumors. Despite its positive correlation with p63 expression in carcinomas in mixed tumors ( r = 0.8323, P < .00001), ΔNp63 expression showed a decrease in benign tumors. Positivity for p53 was detected in 2 of 13 and 1 of 19 benign mixed tumors and carcinomas in mixed tumors, respectively. There was no correlation between p63 or ΔNp63 and p53 expression. Our data support the notion that the decrease of p63 expression, in particular of its isoform ΔNp63, seems to be an important factor in the development of carcinomas in mixed tumors.

scholarly journals Immunohistochemical Expression of Ki67 and p53 in Wilms Tumor and Its Relationship with Tumor Histology and Stage at Presentation

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
O. H. Radhika Krishna ◽  
Geetha Kayla ◽  
Mohammed Abdul Aleem ◽  
Ramani Malleboyina ◽  
Ramesh Reddy Kota
Keyword(s):  
Wilms Tumor ◽  
Tumor Staging ◽  
Treatment Resistance ◽  
Cost Effective ◽  
Proliferation Index ◽  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Tumor Histology ◽  
Chemotherapy Protocol ◽  
Hematoxylin And Eosin

Aim. Evaluate tumor proliferation marker (Ki67) and p53 tumor suppressor marker in Wilms tumor and correlate with histology, anaplasia, and staging. Design. Prospective, hospital based study conducted at a tertiary pediatric referral centre in south India. Setting. Wilms tumor is the most common childhood renal malignancy worldwide. Anaplasia on histology is associated with treatment resistance but not with aggressiveness clinical presentation. Chemotherapy for Wilms tumor is based on histology and staging. Most patients respond to current chemotherapy protocol. However, a small fraction relapses or metastasizes. Affordable prognostic markers are needed for histopathological evaluation of this tumor. Subjects. Cases of histologically confirmed Wilms tumor over five years. Cases after chemotherapy were excluded as the immunostaining was inconsistent in necrotic areas. Methods. The clinical and radiological findings of 31 cases of Wilms tumor were documented at a tertiary pediatric referral hospital over five years. In addition to Hematoxylin and Eosin staining, Ki67 proliferation index and p53 expression were correlated with tumor histology and staging. Results. Age incidence was 3–8 years with female preponderance. Significant correlation was noted between Ki67 proliferation index and tumor staging. p53 expression was not useful in stratification of Wilms tumor. Conclusion. Ki67 was cost-effective immunohistochemical marker for prognostication of pediatric Wilms tumor.

scholarly journals The Prognostic Significance of c-Met and p53 Immunohistochemical Expression in Gastric and Colorectal Carcinomas

2021 ◽  
Vol 9 (A) ◽  
pp. 134-142
Author(s):  
Amany A. Abou-Bakr ◽  
Alshaymaa A. Abdelaziz ◽  
Ibrahim A. Malash ◽  
Osman Mansour ◽  
Ibrahim M. Abdelsalam ◽  
...  
Keyword(s):  
Median Overall Survival ◽  
Potential Role ◽  
Prognostic Significance ◽  
Tumor Stage ◽  
Colorectal Adenomas ◽  
Colorectal Carcinomas ◽  
Immunohistochemical Expression ◽  
P53 Expression ◽  
Gastric Carcinomas ◽  
Gastric Adenocarcinomas

BACKGROUND: Colorectal and gastric carcinomas are the most common and deadly gastrointestinal (GIT) malignancies. AIM: This study aimed to evaluate the expression of c-Met and p53 in gastric and colorectal carcinomas (CRCs) as well as colorectal adenomas using immunohistochemistry. MATERIALS AND METHODS: c-Met and p53 immunohistochemical expression was conducted on 66 cases of gastric adenocarcinomas and total of 60 colonic cases (36 CRCs and 24 colorectal adenomas). RESULTS: In this study, c-Met was positively expressed in 54.5% of gastric carcinomas and 50% of CRCs. In addition, p53 was positively expressed in 56.1% of gastric carcinomas and 72.2% of CRCs. Moreover, higher expression of both c-Met (p = 0.001) and p53 expression (p < 0.001) was reported in CRCs compared to colorectal adenomas. In the same context, c-Met and p53 expressions were positively correlated with intestinal type gastric adenocarcinoma (p < 0.001 and p = 0.03, respectively). Moreover, c-Met was correlated with non-mucinous adenocarcinomas (p = 0.008) and lower grades (p < 0.001) of gastric carcinomas. As regard survival analysis in gastric carcinomas, median overall survival (OS) was better in p53 positive patients (p = 0.05), patients with negative lymph node metastasis (p = 0.03), and patients with better response to neoadjuvant chemotherapy (p = 0.04). In contrast, c-Met did not exhibit significant correlation with OS (p > 0.05). Both c-Met and p53 did not reveal significant correlation with tumor stage and site in both CRCs and gastric carcinomas (p > 0.05). CONCLUSION: We concluded that c-Met and p53 are expressed in the most common GIT malignancies addressing them as potential biomarkers. In addition, c- Met and p53 may have a potential role in colorectal cancer development as they showed higher positivity in CRCs compared to adenomas.

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