Antimicrobial, immunological and biochemical effects of florfenicol and garlic (Allium sativum) on rabbits infected with Escherichia coli serotype O55: H7

2021 ◽  
Author(s):  
Verginia M. Farag ◽  
Reham A. El-Shafei ◽  
Rasha M. Elkenany ◽  
Hanaa S. Ali ◽  
Abdelfattah H. Eladl
Keyword(s):  
Escherichia Coli ◽  
Allium Sativum ◽  
Biochemical Effects

Garlic Extract (Allium sativum L.) Effectively Inhibits Staphylococcus aureus and Escherichia coli by Invitro Test

2020 ◽  
Vol 2 (2) ◽  
pp. 61-68
Author(s):  
Agnina Listya Anggraini ◽  
Ratih Dewi Dwiyanti ◽  
Anny Thuraidah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Infections ◽  
Allium Sativum ◽  
Antibacterial Properties ◽  
Herbal Medicines ◽  
Garlic Extract ◽  
Dilution Method ◽  
Initial Stage

Infection is a disease caused by the presence of pathogenic microbes, including Staphylococcus aureus and Escherichia coli. Garlic (Allium sativum L.) has chemical contents such as allicin, alkaloids, flavonoids, saponins, tannins, and steroids, which can function as an antibacterial against Staphylococcus aureus and Escherichia coli. This study aims to determine the antibacterial properties of garlic extract powder against Staphylococcus aureus and Escherichia coli. This research is the initial stage of the development of herbal medicines to treat Staphylococcus aureus and Escherichia coli infections. The antibacterial activity test was carried out by the liquid dilution method. The concentrations used were 30 mg/mL, 40 mg/mL, 50 mg/mL, 60 mg/mL and 70 mg/mL. The results showed that the Minimum Inhibitory Concentration (MIC) against Staphylococcus aureus and Escherichia coli was 40 mg/mL and 50 mg / mL. Minimum Bactericidal Concentration (MBC) results for Staphylococcus aureus and Escherichia coli are 50 mg/mL and 70 mg/mL. Based on the Simple Linear Regression test, the R2 value of Staphylococcus aureus and Escherichia coli is 0.545 and 0.785, so it can be concluded that there is an effect of garlic extract powder on the growth of Staphylococcus aureus and Escherichia coli by 54.5% and 78.5%. Garlic (Allium sativum L.) extract powder has potential as herbal medicine against bacterial infections but requires further research to determine its effect in vivo.

scholarly journals AНTИМИКРOБНA СВOЈСTВA БИЈEЛOГ ЛУКA (Allium sativum)

2018 ◽  
Vol 15 (1) ◽  
Author(s):  
В. Калаба ◽  
Д. Касагић ◽  
Б. Голић
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Allium Sativum

Ароматичне биљке и етарска уља употребљавају се у хемијско-фармацеут-ској индустрији и апотекама за израду разних љековитих препарата. Бијелилук, због свог састава и особина, има широку примјену у фармацеутској инду-стрији. Састојци бијелог лука имају имунолошки, хемостатички, хемодинамич-ки, фибринолитички, хепатопротективни, антиоксидативни и антиканцеро-гени ефекат. Бијели лук је универзални народни зачин и једно од најважнијихприродних превентивних љековитих средстава. Антимикробна својства бије-лог лука потичу од алицина и сумпорних једињења.Циљ истраживања је утврђивање антимикробне активности екстрак-та бијелог лука на раст бактерија Escherichia coli, Pseudomonas aeruginosa иStaphylococcus aureus.Етарско уље бијелог лука испољило је антимикробна својства према испити-ваним бактеријама.

scholarly journals The Utilization of Garlic (Allium sativum Linn) and Red Ginger (Zingiber officinale var rubra) Extract on The Growth of Broiler were Infected by Escherichia coli

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
A W Isma ◽  
M Tafsin ◽  
Y L Henuk ◽  
N D Hanafi ◽  
Hamdan
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Ginger Extract ◽  
Tetracycline Antibiotics ◽  
E Coli ◽  
Randomized Design ◽  
Red Ginger ◽  
Alternative Antibiotic

This study aims to examine the effect of garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract on the growth of broiler chickens were infected by Escherichia coli. The design used in this study was a complete randomized design (CRD) with 6 treatments and 3 replications. Treatment consists of P0A = Control without infection; P0B = Control + E. coli infection; P1 = Infection E. coli + tetracycline antibiotics 0.05%; P2 = Infection E. coli + garlic extract (Allium sativum Linn) (1%); P3 = Infection E. coli + red ginger extract (Zingiber officinale var rubra) (1%); P4 = Infection E. coli + garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract (1%). The results showed that the effect of garlic and red ginger gave significant influence (P <0,05) to growth of broiler were infected by Escherichia coli. The combination of garlic (Allium sativum Linn) and red ginger (Zingiber officinale var rubra) extract has the highest influence of other activities. It was concluded that giving of garlic and red ginger extract give influence to growth of broiler were infected by Escherichia coli and can be used as alternative antibiotic.

scholarly journals ATIVIDADE ANTIBACTERIANA E MODULADORA DO ÓLEO ESSENCIAL DE Allium sativum L. (Alho)

2020 ◽  
Vol 20 (4) ◽  
Author(s):  
Álife Diêgo Lima SILVA ◽  
Maria de Fátima Guedes MONTEIRO ◽  
Dárcio Luiz de SOUSA JÚNIOR ◽  
Nair Silva MACÊDO ◽  
Cícero Roberto Nascimento SARAIVA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Allium Sativum

O presente estudo tem como objetivo avaliar a atividade antibacteriana e moduladora do óleo essencial de Allium sativum L. (OEAs) associado às luzes de LED. O óleo essencial foi obtido por hidrodestilação e a atividade antibacteriana e moduladora foi executada pelo método de contato gasoso com o uso de luz de LED amarela, azul e vermelha junto com os antibióticos amicacina, ciprofloxacina, gentamicina, norfloxacina, oxaciclina e penicilina. Os testes foram feitos em triplicata e submetidos à análise estatística ANOVA com teste de significância para p<0,05. Para a atividade antibacteriana não houve inibição do crescimento das cepas testadas e para as combinações OEAs + antibiótico + luzes de LED (modulação da resistência antibacteriana) diante de linhagens multirresistentes de Escherichia coli 27 e Staphylococcus aureus 358 o melhor desempenho se mostrou com os antibióticos ciprofloxacina e norfloxacina, respectivamente. Esses resultados tornam-se importantes na colaboração com futuras pesquisas para novas formas de tratamento de doenças provocadas por bactérias multirresistentes.

scholarly journals Antibacterial and antibiofilm effects of garlic (Allium sativum), ginger (Zingiber officinale) and mint (Mentha piperta) on Escherichia coli biofilms

2021 ◽  
Vol 4 (2) ◽  
pp. 166
Author(s):  
Ndaindila Haindongo ◽  
Amara Anyogu ◽  
Osmond Ekwebelem ◽  
Christian Anumudu ◽  
Helen Onyeaka
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Food Industry ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Bacterial Survival ◽  
Planktonic Cells ◽  
E Coli ◽  
Foodborne Outbreaks ◽  
Potential Use

Biofilms are a significant concern in the food industry because of their potential to enhance bacterial survival and cause foodborne outbreaks. Escherichia coli (E. coli) is among the leading pathogens responsible for foodborne outbreaks and this can be attributed to its ability to form biofilms in food containers and food preparatory surfaces. The purpose of this study was to investigate the antibacterial and antibiofilm properties of garlic, ginger and mint and their potential to inhibit E.coli and biofilm formation. Disc diffusion assays and 96-well plate crystal violet-based methods were used to achieve these objectives. The plant extracts were diluted from 1 mg/ml to 0.1 mg/ml and incubated 25°C and 37°C to investigate the antimicrobial and antibiofilm effects on E. coli. The findings of this study showed that low temperatures induced the formation of E. coli biofilms and all tested extracts contain a broad spectrum of antibacterial and antibiofilm properties. This study provided new insights on the combined antimicrobial and antibiofilm properties of garlic, ginger and mint against planktonic cells and biofilms of E. coli MG 1655 and highlight the potential use of these extracts in the food industry to prevent biofilm formation by E. coli. 

Đánh giá mức độ nhiễm vi sinh vật gây bệnh có trong thịt heo và các yếu tố ảnh hưởng đến tính kháng Escherichia coli của tỏi (Allium sativum L.)

2019 ◽  
Vol 55(Công nghệ Sinh học) ◽  
pp. 185
Author(s):  
Huỳnh Ngọc Thanh Tâm ◽  
Nguyễn Thị Minh Trâm ◽  
Đinh Thị Tuyết Phương ◽  
Nguyễn Thị Mộng Tuyền ◽  
Phan Thị Thu Sương ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Allium Sativum

scholarly journals Phytobiotic Properties of Garlic, Red Ginger, Turmeric and Kencur in Growing Ducks

2015 ◽  
Vol 17 (1) ◽  
pp. 49 ◽  
Author(s):  
Ismoyowati Ismoyowati ◽  
Diana Indrasanti ◽  
Mochamad Mufti ◽  
Abdoreza Soleimani Farjam
Keyword(s):  
Escherichia Coli ◽  
Allium Sativum ◽  
Zingiber Officinale ◽  
Feed Conversion ◽  
Feeding Trial ◽  
E Coli ◽  
Salmonella Pullorum ◽  
Red Ginger

Abstract. Phytobiotic properties of garlic (Allium sativum), turmeric (Curcuma domestica), red ginger (Zingiber officinale) and kencur (Kaempferia galangal) were studied using standard in vitro antibacterial test and in vivo feeding trial with ducklings. In the in vitro experiment, potency of aqueous extract of these phytobiotic agents were tested against Salmonella pullorum and Escherichia coli. Feeding trial was carried out for 6 week starting at day 28 using ducklings fed diets supplemented with 1% of each of four phytobiotic agents. The highest antibacterial activity against S. pullorum and E. coli was observed with garlic and no additive effect when mixture of phytobiotics was used. Weight gain, fed intake and feed conversion ratio of ducklings were not affected by inclusion of garlic, red ginger and kencur. However, 1% turmeric supplementation significantly reduced growth performance to ducklings. Key words: phytobiotic, antibiotic, duck, medicinal plants Abstrak.  Penelitian karakteristik fitobiotik dari bawang putih (Allium sativum), kunyit (Curcuma domestica), jahe merah (Zingiber officinale) dan kencur (Kaempferia galangal) telah dilakukan secara in vitro melalui uji aktivitas antibakteri dan secara in vivo dengan perlakuan suplemetasi fitobiotik didalam pakan anak itik. Pada percobaan  in vitro, potensi  aktivitas antibakteri dari ektrak  fitobiotik diuji menggunakan Salmonella pullorum and Escherichia coli.  Percobaan suplementasi fitobiotik diberikan masing-masing sebesar 1% didalam pakan anak itik.  Pemberian pakan perlakuan dilakukan selama 6 minggu,  dimulai pada saat anak itik berumur 28 hari.  Hasil penelitian menunjukkan aktivitas antibakteri terhadap S. pullorum dan E. coli paling tinggi adalah ektrak bawang putih dan tidak ada pengaruh yang lebih baik apabila dicampur dengan ektrak fitobiotik lainnya.  Pertambahan bobot badan, konsumsi pakan dan konversi pakan anak itik tidak dipengaruhi oleh penambahan bawang putih, kunyit, jahe merah dan kencur.  Akan tetapi, suplementasi kunyit nyata menurunkan performan pertumbuhan anak itik. Kata kunci: fitobiotik, antibiotik, itik, tanaman obat-obatan.

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