Biosurfactant production by the crude oil degrading Stenotrophomonas sp. B-2: chemical characterization, biological activities and environmental applications

Cultivation of mint timija (Mentha suaveolens subsp. timija (Briq.) Harley) constitutes a promising solution to the conservation and sustainable utilization of this Moroccan endemic and threatened species. Optimized agronomic practices require mineral and/or biological fertilizer applications. The aim of this study was to determine the effects of application of a complete (N, P, and K) mineral fertilizer and vesicular arbuscular mycorrhizae (VAM) inoculation on the composition, antioxidant, and insecticidal properties of mint timija essential oils (EOs). The GC-MS analyses identified 27 components representing more than 99.9% of the total oils. Menthone (40.7–49.3%), pulegone (31.3–36.5%), and isomenthone (2.5–4.4%) were found to be the main constituents. Cultivation of mint timija with mineral fertilizer and VAM inoculation induced an increase in menthone content and a parallel decrease of pulegone. Both treatments enhanced the antioxidant activity of the investigated EOs in all assays (IC50 ranged from 2.34 ± 0.03 mg/mL to 6.82 ± 0.25 mg/mL), while no significant difference in the toxicities of these oils against Tribolium confusum du Val. has been observed. Overall, we conclude that cultivation using complete mineral fertilizer and VAM inoculation could be useful in modulating the chemical composition and enhancing the antioxidant activity of the EO of this endemic Moroccan species.

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Preparation and physico-chemical characterization of β-cyclodextrin incorporated chitosan biosorbent beads with potential environmental applications

Materials Research Express ◽

10.1088/2053-1591/aac707 ◽

2018 ◽

Vol 5 (6) ◽

pp. 065503 ◽

Cited By ~ 3

Author(s):

Somayyah Abdul Munim ◽

Muhammad Tahir Saddique ◽

Zulfiqar Ali Raza ◽

Muhammad Irfan Majeed

Keyword(s):

Chemical Characterization ◽

Environmental Applications ◽

Physico Chemical

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A novel Synthesis, Chemical Characterization and Biological Activities of Metal-Leather Protein Hydrolysate Chelates

10.21203/rs.3.rs-172783/v1 ◽

2021 ◽

Author(s):

Rania Jacob ◽

Hazem Hassan ◽

Adel Afify ◽

Gamal Gabr

Keyword(s):

Melting Point ◽

Protein Hydrolysate ◽

Biological Activities ◽

Chemical Characterization ◽

Antibacterial Activities ◽

Biochemical Properties ◽

Mineral Salts ◽

Mineral Absorption ◽

E Coli ◽

Leather Processing

Abstract Leather industries covers a wide chain of production and indirectly contributes to the economic flow. The different stages used in leather processing led to produce huge solid waste volumes. Because of the great effectiveness of amino acids as naturally chelates for minerals, the present study was carried out to recycling leather waste into its protein hydrolysate by CaO hydrolysis. The Leather protein hydrolysates (LPHs) was used to prepare metal-leather protein hydrolysate chelates (Cu2+-, Zn2+-& Fe2+-LPHCs) and some of their physical properties (i.e. λ-max, FTIR spectra, color, melting point) and biochemical properties as its antibacterial activity, as well as using as micronutrient elements for plant were evaluated. Results showed that the Cu2+-LPHC gave the highest value of melting point and λ-max than other chelates. All chelates shifted the vibration bands toward a higher frequency than LPH/CaO. Metal-leather protein hydrolysate (M-LPHCs) had antibacterial activities against E. coli, B. cereus and Micrococcus spp. mostly with Zn-LPHC and Fe-LPHC. These complexes also increased the growth characteristics and mineral absorption of spinach plants in hydroponic nutrient solution than that of mineral salts (CuSO4, ZnSO4 and FeSO4). Finally, the study concluded that M-LPHCs can be used as antimicrobial agent, micronutrients for plant and support the minerals bioavailability in animals.

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Parallelism of Chemicostructural Properties between Filgrastim Originator and Three of Its Biosimilar Drugs

Journal of Chemistry ◽

10.1155/2019/2751461 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15 ◽

Cited By ~ 2

Author(s):

Alessandra Gianoncelli ◽

Michela Bertuzzi ◽

Michela Guarienti ◽

Sara Vezzoli ◽

Sara Anna Bonini ◽

...

Keyword(s):

Biological Activity ◽

Reference Product ◽

Biological Activities ◽

Chemical Characterization ◽

Structural Similarity ◽

Functional Assay ◽

Biological Drugs ◽

Reference Drug ◽

Rp Hplc

The approval and granting of marketing authorization for a putative biosimilar is based on strong comparability studies with its biological reference product. This is due to the complexity of the structure and nature of the manufacturing process of biological drugs. Hence, a rigorous analytical workflow for chemical characterization and clinical trials to evaluate the efficacy and safety is required to demonstrate their high similarities to the reference drug. This work is focused on the comparison of the originator of filgrastim with three of its biosimilars by evaluating their structural similarity and biological activity. Qualiquantitative analyses were performed by MALDI-TOF/TOF-MS and RP-HPLC-UV. An innovative functional assay using zebrafish as the animal model was developed to evaluate the biological activities of the drugs. The different analyses performed in this study highlighted the structural similarity of biosimilar drugs and their originator. This result was further confirmed by a similar in vivo biological activity.

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